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TPCN1 (NM_001143819) Human Tagged ORF Clone
CAT＃: RG227015
- TrueORF®
TPCN1 (tGFP-tagged) - Human two pore segment channel 1 (TPCN1), transcript variant 1
ORF Plasmid: DDK
"NM_001143819" in other vectors (4)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | TPC1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG227015 representing NM_001143819
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCTTGCTACATTGCCCAGGCTGGTCTTGAACTGCTAGGCTCAAGCAGTTCTCCCACCTTGACCT CCCAAAGCGCTGAGATTACAGAAGATGCCTCTAATGGAGGAGTTTCTGAGCAGCACCCCTGGCCCAGTGG CTTTGAAAGGGAGCTCAAACCAGAGACTATTTCAAGCCCTGGATATCATATCCTGAGGGCCACAGGAGAA GAGAACATGGCTGTGAGTTTGGATGACGACGTGCCGCTCATCCTGACCTTGGATGAGGGTGGCAGTGCCC CACTGGCTCCCTCCAACGGCCTGGGCCAAGAAGAGCTACCTAGCAAAAATGGCGGCAGCTATGCCATCCA CGACTCCCAGGCCCCCAGTCTCAGCTCTGGGGGTGAGAGTTCCCCCTCCAGCCCCGCACACAACTGGGAG ATGAATTACCAAGAGGCAGCAATCTACCTCCAGGAAGGCGAGAACAACGACAAGTTCTTCACCCACCCCA AGGATGCCAAGGCGCTGGCGGCCTACCTCTTTGCACACAATCACCTCTTCTACCTGATGGAGCTGGCCAC GGCCCTGCTGCTGCTGCTGCTCTCCCTGTGCGAGGCCCCCGCCGTCCCCGCACTCCGGCTTGGCATCTAT GTCCACGCCACCCTGGAGCTGTTTGCCCTGATGGTGGTAGTGTTTGAACTCTGCATGAAGTTACGCTGGC TGGGCCTCCACACCTTCATCCGGCACAAGCGGACCATGGTCAAGACCTCGGTGCTGGTGGTGCAGTTTGT CGAGGCCATCGTGGTGTTGGTACGGCAGATGTCCCATGTGCGGGTGACCCGAGCACTGCGCTGCATTTTC CTGGTGGACTGTCGGTATTGCGGTGGCGTCCGGCGCAACCTGCGGCAGATCTTCCAGTCCCTGCCGCCCT TCATGGACATCCTCCTGCTGCTGCTGTTCTTCATGATCATCTTTGCCATCCTCGGTTTCTACTTGTTCTC CCCTAACCCTTCAGACCCCTACTTCAGCACCCTGGAGAACAGCATCGTCAGTCTGTTTGTCCTTCTGACC ACAGCCAATTTCCCAGATGTGATGATGCCCTCCTACTCCCGGAACCCCTGGTCCTGCGTCTTCTTCATCG TGTACCTCTCCATCGAGCTGTATTTCATCATGAACCTGCTTCTGGCTGTGGTGTTCGACACCTTCAATGA CATTGAGAAACGCAAGTTCAAGTCTTTGCTACTGCACAAGCGAACCGCTATCCAGCATGCCTACCGCCTG CTCATCAGCCAGAGGAGGCCTGCCGGCATCTCCTACAGGCAGTTTGAAGGCCTCATGCGCTTCTACAAGC CCCGGATGAGTGCCAGGGAGCGCTATCTTACCTTCAAGGCCCTGAATCAGAACAACACACCCCTGCTCAG CCTAAAGGACTTTTACGATATCTACGAAGTTGCTGCTTTGAAGTGGAAGGCCAAGAAAAACAGAGAGCAC TGGTTTGATGAGCTTCCCAGGACGGCGCTCCTCATCTTCAAAGGTATTAATATCCTTGTGAAGTCCAAGG CCTTCCAGTATTTCATGTACTTGGTGGTGGCAGTCAACGGGGTCTGGATCCTCGTGGAGACATTTATGCT GAAAGGTGGGAACTTCTTCTCCAAGCACGTGCCCTGGAGTTACCTCGTCTTTCTAACTATCTATGGGGTG GAGCTGTTCCTGAAGGTTGCCGGCCTGGGCCCTGTGGAGTACTTGTCTTCCGGATGGAACTTGTTTGACT TCTCCGTGACAGTGTTCGCCTTCCTGGGACTGCTGGCGCTGGCCCTCAACATGGAGCCCTTCTATTTCAT CGTGGTCCTGCGCCCCCTCCAGCTGCTGAGGTTGTTTAAGTTGAAGGAGCGCTACCGCAACGTGCTGGAC ACCATGTTCGAGCTGCTGCCCCGGATGGCCAGCCTGGGCCTCACCCTGCTCATCTTTTACTACTCCTTCG CCATCGTGGGCATGGAGTTCTTCTGCGGGATCGTCTTCCCCAACTGCTGCAACACGAGTACAGTGGCAGA TGCCTACCGCTGGCGCAACCACACCGTGGGCAACAGGACCGTGGTGGAGGAAGGCTACTATTATCTCAAT AATTTTGACAACATCCTCAACAGCTTTGTGACCCTGTTTGAGCTCACAGTTGTCAACAACTGGTACATCA TCATGGAAGGCGTCACCTCTCAGACCTCCCACTGGAGCCGCCTCTACTTCATGACCTTTTACATTGTGAC CATGGTGGTGATGACGATCATTGTCGCCTTTATCCTCGAGGCCTTCGTCTTCCGAATGAACTACAGCCGC AAGAACCAGGACTCGGAAGTTGATGGTGGCATCACCCTTGAGAAGGAAATCTCCAAAGAAGAGCTGGTTG CCGTCCTGGAGCTCTACCGGGAGGCACGGGGGGCCTCCTCGGATGTCACCAGGCTGCTGGAGACCCTCTC CCAGATGGAGAGATACCAGCAACATTCCATGGTGTTTCTGGGACGGCGATCAAGGACCAAGAGCGACCTG AGCCTGAAGATGTACCAGGAGGAGATCCAGGAGTGGTATGAGGAGCATGCCAGGGAGCAAGAGCAGCAGC GACAACTCAGCAGCAGTGCAGCCCCCGCCGCCCAGCAGCCCCCAGGCAGCCGCCAGCGCTCCCAGACCGT TACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG227015 representing NM_001143819
Red=Cloning site Green=Tags(s) MESCYIAQAGLELLGSSSSPTLTSQSAEITEDASNGGVSEQHPWPSGFERELKPETISSPGYHILRATGE ENMAVSLDDDVPLILTLDEGGSAPLAPSNGLGQEELPSKNGGSYAIHDSQAPSLSSGGESSPSSPAHNWE MNYQEAAIYLQEGENNDKFFTHPKDAKALAAYLFAHNHLFYLMELATALLLLLLSLCEAPAVPALRLGIY VHATLELFALMVVVFELCMKLRWLGLHTFIRHKRTMVKTSVLVVQFVEAIVVLVRQMSHVRVTRALRCIF LVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFVLLT TANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYRL LISQRRPAGISYRQFEGLMRFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAKKNREH WFDELPRTALLIFKGINILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYLVFLTIYGV ELFLKVAGLGPVEYLSSGWNLFDFSVTVFAFLGLLALALNMEPFYFIVVLRPLQLLRLFKLKERYRNVLD TMFELLPRMASLGLTLLIFYYSFAIVGMEFFCGIVFPNCCNTSTVADAYRWRNHTVGNRTVVEEGYYYLN NFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSR KNQDSEVDGGITLEKEISKEELVAVLELYREARGASSDVTRLLETLSQMERYQQHSMVFLGRRSRTKSDL SLKMYQEEIQEWYEEHAREQEQQRQLSSSAAPAAQQPPGSRQRSQTVT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001143819 |
| ORF Size | 2664 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001143819.3 |
| RefSeq Size | 5472 bp |
| RefSeq ORF | 2667 bp |
| Locus ID | 53373 |
| UniProt ID | Q9ULQ1 |
| Protein Families | Druggable Genome, Ion Channels: Other, Transmembrane |
| Gene Summary | Voltage-gated Ca(2+) and Na+ channels have 4 homologous domains, each containing 6 transmembrane segments, S1 to S6. TPCN1 is similar to these channels, but it has only 2 domains containing S1 to S6 (Ishibashi et al., 2000 [PubMed 10753632]).[supplied by OMIM, Mar 2008] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227015 | TPCN1 (Myc-DDK-tagged)-Human two pore segment channel 1 (TPCN1), transcript variant 1 |
CNY 6,504.00 |
|
| RC227015L3 | Lenti ORF clone of Human two pore segment channel 1 (TPCN1), transcript variant 1, Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC227015L4 | Lenti ORF clone of Human two pore segment channel 1 (TPCN1), transcript variant 1, mGFP tagged |
CNY 8,904.00 |
|
| SC326270 | TPCN1 (untagged)-Human two pore segment channel 1 (TPCN1), transcript variant 1 |
CNY 17,100.00 |
