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PCDH15 (NM_001142769) Human Tagged ORF Clone
CAT＃: RG226910
- TrueORF®
PCDH15 (tGFP-tagged) - Human protocadherin-related 15 (PCDH15), transcript variant I
ORF Plasmid: DDK
"NM_001142769" in other vectors (2)
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CNY 19,670.00
货期*
8周
规格
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经常一起买 (5)
PCDH15 mouse monoclonal antibody,clone OTI7F10
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CDHR15; DFNB23; USH1F |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG226910 representing NM_001142769
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCGACAGTTTTATCTCTGGACATGTTTAGCTTCAGGGATCATCCTGGGCTCTCTCTTTGAAATCT GCTTGGGCCAGTATGATGATGACTGGCAATATGAGGATTGCAAACTAGCTAGGGGAGGACCACCAGCTAC CATAGTTGCTATTGATGAAGAAAGTCGGAATGGTACAATTCTGGTGGACAACATGCTGATCAAAGGGACT GCTGGAGGACCAGACCCCACCATAGAACTTTCTTTAAAGGATAATGTGGATTACTGGGTGTTGATGGATC CTGTTAAGCAAATGCTTTTCCTGAACAGCACCGGAAGAGTTCTGGATAGAGATCCACCGATGAACATACA CTCCATTGTGGTGCAGGTCCAGTGCATCAACAAAAAAGTGGGCACTATTATCTACCATGAAGTGCGAATA GTGGTGAGAGACAGGAATGACAACTCACCCACTTTCAAGCATGAAAGCTACTATGCCACAGTGAATGAGC TCACTCCAGTTGGTACCACAATATTCACAGGATTTTCAGGAGACAATGGAGCTACAGATATAGATGATGG ACCAAATGGACAGATAGAGTATGTTATTCAGTATAATCCAGATGATCCGACATCCAATGACACCTTTGAA ATTCCCCTAATGTTGACTGGAAATATAGTGTTAAGGAAGAGGCTCAACTATGAAGATAAGACTCGCTACT TTGTCATAATCCAAGCTAATGACCGTGCCCAAAATCTGAATGAGAGGCGAACCACCACCACCACTCTCAC AGTGGATGTTCTGGATGGAGATGACTTGGGTCCAATGTTTCTTCCTTGTGTCCTTGTGCCAAACACTCGT GATTGCCGTCCACTCACTTATCAAGCTGCCATACCTGAGTTGAGAACTCCGGAAGAACTGAACCCCATTA TTGTTACGCCACCAATCCAAGCCATTGATCAGGACCGGAATATTCAACCGCCATCAGATAGGCCAGGAAT CCTCTATTCCATCCTTGTTGGGACTCCTGAGGATTACCCACGATTTTTCCATATGCATCCTAGGACAGCA GAACTTAGTCTCCTGGAGCCAGTAAACAGAGACTTTCACCAGAAATTTGATTTGGTTATTAAGGCTGAAC AAGACAATGGTCATCCTCTTCCTGCCTTTGCCGGTCTACACATTGAAATACTGGATGAAAACAATCAAAG TCCATATTTTACAATGCCCAGTTATCAAGGCTATATCCTGGAATCTGCCCCAGTGGGAGCAACCATTTCG GACAGTCTCAATTTGACTTCACCTTTAAGAATAGTAGCTCTGGACAAGGACATAGAAGATGTTCCACCCA GTGGAGTTCCTACAAAAGACCCAGAGCTTCACCTTTTTCTGAATGACTACACCTCAGTCTTCACCGTCAC ACAGACTGGTATTACTCGCTACCTCACCTTACTTCAACCAGTGGACAGGGAAGAACAGCAAACTTACACC TTTTCGATAACAGCATTTGATGGTGTACAAGAAAGTGAGCCAGTCATCGTCAATATTCAAGTGATGGATG CAAATGATAACACGCCAACCTTCCCTGAAATATCCTATGATGTGTATGTTTATACAGACATGAGACCTGG GGACAGTGTCATACAGCTCACTGCAGTCGACGCAGACGAAGGGTCAAATGGGGAGATCACATATGAAATC CTTGTTGGGGCTCAGGGAGACTTCATCATCAATAAAACAACAGGGCTTATCACCATCGCTCCAGGGGTGG AAATGATAGTCGGGCGGACTTACGCACTCACGGTCCAAGCAGCGGATAATGCTCCTCCTGCAGAGCGAAG GAACTCCATCTGCACTGTGTATATTGAAGTGCTTCCACCAAATAATCAAAGCCCTCCTCGCTTCCCACAG CTGATGTATAGCCTTGAAATTAGTGAAGCCATGAGGGTTGGTGCTGTTTTATTAAATCTACAGGCAACTG ATCGAGAGGGAGACTCAATAACATATGCCATTGAGAATGGAGATCCTCAGAGAGTTTTTAATCTTTCAGA AACCACGGGGATTCTAACCTTAGGGAAAGCACTGGACAGGGAAAGCACTGATCGCTACATTCTGATCATC ACAGCTTCAGATGGCAGGCCAGATGGGACCTCAACTGCCACAGTAAACATAGTGGTGACAGATGTCAATG ACAATGCTCCAGTGTTTGATCCTTATCTGCCAAGAAATTTATCTGTGGTGGAAGAAGAAGCCAATGCCTT TGTGGGTCAAGTAAAAGCAACAGACCCTGATGCTGGAATAAATGGTCAAGTGCACTACAGTTTGGGTAAC TTTAATAATCTTTTTCGTATCACATCCAATGGGAGCATTTACACAGCAGTGAAGCTTAACAGAGAAGTCA GGGACTACTATGAACTTGTTGTTGTGGCAACAGATGGAGCAGTACACCCTCGTCATTCAACTCTAACCTT GGCCATCAAGGTTTTGGACATTGATGATAACAGTCCTGTGTTCACCAATTCAACATACACTGTCCTTGTT GAAGAGAATTTGCCAGCTGGGACTACCATCCTTCAAATAGAGGCCAAAGATGTCGACCTTGGAGCAAATG TGTCTTACCGGATAAGAAGCCCAGAAGTGAAGCACTTTTTTGCACTACATCCATTTACAGGAGAACTATC GCTTTTAAGGAGTTTAGATTATGAGGCATTTCCAGACCAAGAAGCAAGTATCACTTTTCTGGTAGAGGCC TTTGATATTTATGGAACAATGCCACCTGGTATTGCTACTGTCACAGTGATTGTAAAGGATATGAATGATT ATCCTCCTGTCTTTAGTAAACGAATATACAAAGGGATGGTGGCTCCGGATGCAGTCAAGGGTACACCTAT CACAACAGTTTATGCTGAAGATGCAGACCCTCCTGGATTACCTGCAAGTCGTGTGAGGTATAGAGTAGAT GATGTACAGTTTCCTTACCCTGCCAGTATTTTTGAAGTGGAAGAAGATTCTGGAAGAGTAATAACACGAG TCAATCTTAATGAAGAACCTACAACAATTTTTAAGTTGGTGGTGGTTGCTTTTGATGATGGGGAGCCTGT GATGTCCAGCAGTGCCACAGTGAAGATTCTTGTCTTACATCCTGGTGAGATCCCACGCTTCACACAGGAG GAATATAGACCTCCTCCAGTAAGTGAACTTGCCACCAAAGGGACCATGGTTGGTGTAATTTCTGCTGCTG CCATTAATCAAAGTATTGTGTACTCCATTGTTTCAGGAAATGAAGAAGATACATTTGGAATTAATAACAT CACAGGTGTTATCTATGTGAATGGACCTCTGGATTATGAGACCAGGACAAGCTATGTACTTCGAGTCCAA GCTGATTCCCTGGAAGTGGTCCTTGCCAATCTCCGAGTTCCTTCAAAAAGCAATACAGCTAAAGTATACA TTGAGATTCAGGATGAAAATAATCATCCCCCAGTGTTTCAGAAAAAATTCTACATCGGAGGTGTATCTGA AGATGCAAGAATGTTTACTTCTGTACTCAGAGTGAAGGCTACTGATAAAGATACTGGCAATTATAGTGTC ATGGCCTACAGACTCATAATACCACCAATTAAAGAGGGAAAAGAAGGATTTGTAGTGGAAACATATACAG GGCTTATCAAAACTGCTATGCTCTTCCATAATATGAGGAGATCCTACTTCAAGTTTCAAGTTATTGCAAC TGACGACTATGGGAAGGGACTGAGCGGCAAAGCCGATGTACTCGTCTCCGTGGTCAATCAGCTGGATATG CAAGTCATTGTTTCCAATGTGCCTCCTACTCTAGTGGAAAAAAAGATAGAAGATCTTACAGAGATCTTGG ATCGCTATGTTCAGGAACAAATTCCTGGTGCCAAGGTCGTAGTGGAGTCCATTGGAGCTCGCCGGCATGG AGATGCCTTTTCCCTAGAAGATTACACCAAATGTGACTTGACTGTCTATGCAATTGACCCCCAAACCAAC AGAGCCATCGATAGAAATGAGCTTTTTAAATTTTTGGATGGCAAACTACTTGATATCAATAAAGACTTTC AGCCGTATTATGGGGAAGGAGGACGCATTCTGGAGATCCGGACTCCAGAGGCAGTGACCAGCATTAAAAA GAGAGGAGAAAGTCTAGGATACACAGAAGGGGCCTTGTTGGCTCTGGCCTTCATCATCATCCTCTGCTGC ATTCCTGCCATCTTGGTGGTTTTGGTCAGCTACAGACAGTTTAAAGTACGTCAAGCTGAGTGTACAAAGA CTGCACGAATTCAGGCCGCATTACCCGCGGCTAAACCAGCAGTGCCGGCTCCTGCACCAGTGGCAGCGCC CCCGCCGCCGCCGCCGCCTCCGCCAGGTGCGCATCTCTATGAAGAACTTGGAGACAGCTCAATGCATAAG TATGAAATGCCTCAATATGGGAGTCGCCGTCGATTGTTACCACCAGCTGGACAGGAGGAATATGGTGAGG TGGTTGGTGAAGCTGAGGAAGAATATGAGGAGGAAGAGGAAGAGCCAAAGAAAATTAAAAAACCAAAGGT TGAAATTAGAGAGCCTAGTGAGGAGGAAGAAGTAGTTGTAACTATCGAAAAACCACCAGCAGCTGAGCCT ACATACACAACATGGAAGAGAGCCAGAATATTCCCCATGATTTTTAAGAAAGTTAGAGGATTAGCTGATA AAAGAGGAATCGTTGACCTTGAGGGTGAAGAGTGGCAGAGACGCCTTGAGGAAGAAGATAAAGATTATTT GAAACTCACTCTGGACCAAGAGGAAGCAACAGAAAGCACTGTAGAATCAGAGGAGGAATCCTCCAGCGAC TATACTGAATACAGTGAAGAAGAGTCTGAGTTCAGTGAGTCTGAGACTACAGAAGAGGAATCTGAGTCAG AGACACCCTCTGAGGAGGAGGAGAGTTCCACCCCTGAATCAGAAGAATCGGAATCCACAGAGTCAGAAGG AGAAAAAGCAAGGAAAAACATTGTGCTTGCAAGAAGAAGGCCCATGGTTGAGGAGGTCAAGGAAGTCAAG GGTAGGAAAGAGGAGCCACAAGAAGAACAAAAAGAACCTAAGATGGAAGAAGAAGAACACTCAGAAGAAG AAGAAAGTGGACCAGCCCCTGTGGAAGAAAGTACAGACCCTGAAGCTCAAGATATCCCTGAAGAGGGCAG TGCAGAATCAGCTTCGGTGGAAGGAGGTGTGGAAAGTGAGGAGGAATCAGAATCAGGTAGTAGTAGCAGT AGTAGCGAAAGTCAGTCTGGAGGTCCATGGGGCTATCAGGTACCAGCGTATGACAGAAGCAAGAATGCAA ACCAAAAGAAGTCGCCAGGAGCAAACTCTGAAGGTTACAACACAGCACTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226910 representing NM_001142769
Red=Cloning site Green=Tags(s) MFRQFYLWTCLASGIILGSLFEICLGQYDDDWQYEDCKLARGGPPATIVAIDEESRNGTILVDNMLIKGT AGGPDPTIELSLKDNVDYWVLMDPVKQMLFLNSTGRVLDRDPPMNIHSIVVQVQCINKKVGTIIYHEVRI VVRDRNDNSPTFKHESYYATVNELTPVGTTIFTGFSGDNGATDIDDGPNGQIEYVIQYNPDDPTSNDTFE IPLMLTGNIVLRKRLNYEDKTRYFVIIQANDRAQNLNERRTTTTTLTVDVLDGDDLGPMFLPCVLVPNTR DCRPLTYQAAIPELRTPEELNPIIVTPPIQAIDQDRNIQPPSDRPGILYSILVGTPEDYPRFFHMHPRTA ELSLLEPVNRDFHQKFDLVIKAEQDNGHPLPAFAGLHIEILDENNQSPYFTMPSYQGYILESAPVGATIS DSLNLTSPLRIVALDKDIEDVPPSGVPTKDPELHLFLNDYTSVFTVTQTGITRYLTLLQPVDREEQQTYT FSITAFDGVQESEPVIVNIQVMDANDNTPTFPEISYDVYVYTDMRPGDSVIQLTAVDADEGSNGEITYEI LVGAQGDFIINKTTGLITIAPGVEMIVGRTYALTVQAADNAPPAERRNSICTVYIEVLPPNNQSPPRFPQ LMYSLEISEAMRVGAVLLNLQATDREGDSITYAIENGDPQRVFNLSETTGILTLGKALDRESTDRYILII TASDGRPDGTSTATVNIVVTDVNDNAPVFDPYLPRNLSVVEEEANAFVGQVKATDPDAGINGQVHYSLGN FNNLFRITSNGSIYTAVKLNREVRDYYELVVVATDGAVHPRHSTLTLAIKVLDIDDNSPVFTNSTYTVLV EENLPAGTTILQIEAKDVDLGANVSYRIRSPEVKHFFALHPFTGELSLLRSLDYEAFPDQEASITFLVEA FDIYGTMPPGIATVTVIVKDMNDYPPVFSKRIYKGMVAPDAVKGTPITTVYAEDADPPGLPASRVRYRVD DVQFPYPASIFEVEEDSGRVITRVNLNEEPTTIFKLVVVAFDDGEPVMSSSATVKILVLHPGEIPRFTQE EYRPPPVSELATKGTMVGVISAAAINQSIVYSIVSGNEEDTFGINNITGVIYVNGPLDYETRTSYVLRVQ ADSLEVVLANLRVPSKSNTAKVYIEIQDENNHPPVFQKKFYIGGVSEDARMFTSVLRVKATDKDTGNYSV MAYRLIIPPIKEGKEGFVVETYTGLIKTAMLFHNMRRSYFKFQVIATDDYGKGLSGKADVLVSVVNQLDM QVIVSNVPPTLVEKKIEDLTEILDRYVQEQIPGAKVVVESIGARRHGDAFSLEDYTKCDLTVYAIDPQTN RAIDRNELFKFLDGKLLDINKDFQPYYGEGGRILEIRTPEAVTSIKKRGESLGYTEGALLALAFIIILCC IPAILVVLVSYRQFKVRQAECTKTARIQAALPAAKPAVPAPAPVAAPPPPPPPPPGAHLYEELGDSSMHK YEMPQYGSRRRLLPPAGQEEYGEVVGEAEEEYEEEEEEPKKIKKPKVEIREPSEEEEVVVTIEKPPAAEP TYTTWKRARIFPMIFKKVRGLADKRGIVDLEGEEWQRRLEEEDKDYLKLTLDQEEATESTVESEEESSSD YTEYSEEESEFSESETTEEESESETPSEEEESSTPESEESESTESEGEKARKNIVLARRRPMVEEVKEVK GRKEEPQEEQKEPKMEEEEHSEEEESGPAPVEESTDPEAQDIPEEGSAESASVEGGVESEEESESGSSSS SSESQSGGPWGYQVPAYDRSKNANQKKSPGANSEGYNTAL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001142769 |
| ORF Size | 5370 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001142769.3 |
| RefSeq Size | 5768 bp |
| RefSeq ORF | 5373 bp |
| Locus ID | 65217 |
| Protein Families | Druggable Genome, Transmembrane |
| Gene Summary | This gene is a member of the cadherin superfamily. Family members encode integral membrane proteins that mediate calcium-dependent cell-cell adhesion. It plays an essential role in maintenance of normal retinal and cochlear function. Mutations in this gene result in hearing loss and Usher Syndrome Type IF (USH1F). Extensive alternative splicing resulting in multiple isoforms has been observed in the mouse ortholog. Similar alternatively spliced transcripts are inferred to occur in human, and additional variants are likely to occur. [provided by RefSeq, Dec 2008] |
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