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PTPH1 (PTPN3) (NM_001145370) Human Tagged ORF Clone
CAT＃: RG226819
- TrueORF®
PTPN3 (tGFP-tagged) - Human protein tyrosine phosphatase, non-receptor type 3 (PTPN3), transcript variant 4
ORF Plasmid: DDK
"NM_001145370" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PTP-H1; PTPH1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG226819 representing NM_001145370
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCTCCCGGTTACGTGCGTTGGGTGGAAGAATTAATAATATACGCACCTCGGAGTTACCCAAAGAGA AAACTCGATCAGAAGTCATTTGCAGCATCCACTTTTTAGATGGCGTGGTACAGACCTTTAAAGTTACTAA ACAAGACACTGGCCAGGTTCTTCTGGATATGGTGCACAACCACCTGGGTGTGACTGAAAAGGAATATTTT GGTTTACAGCATGATGACGACTCCGTGGACTCTCCTAGATGGCTGGAAGCAAGCAAAGCCATCAGGAAGC AGTTAAAAGGAGGTTTCCCCTGTACCCTGCATTTTCGAGTAAGATTTTTTATACCTGATCCCAACACACT GCAGCAAGAACAAACCAGGCACTTGTATTTCTTACAACTGAAGATGGATATTTGCGAAGGAAGGTTAACC TGCCCTCTTAACTCAGCAGTGGTTCTAGCGTCCTATGCCGTACAATCTCATTTTGGAGACTATAATTCTT CCATACATCATCCAGGCTATCTTTCCGATAGTCACTTTATACCCGATCAAAATGAGGACTTTTTAACAAA AGTCGAATCTCTGCATGAGCAGCACAGTGGGCTAAAACAATCAGAAGCAGAATCCTGCTATATCAACATA GCGCGGACCCTCGACTTCTATGGAGTAGAACTGCACAGTGGTAGGGATCTGCACAATTTAGACCTAATGA TTGGAATTGCTTCCGCGGGTGTTGCTGTGTACCGAAAATACATTTGCACAAGTTTCTATCCTTGGGTGAA CATTCTCAAAATTTCTTTCAAAAGGAAAAAGTTCTTCATACATCAGCGACAGAAACAGGCTGAATCCAGG GAACATATTGTGGCCTTCAACATGCTGAATTACCGATCTTGCAAAAACTTGTGGAAATCCTGTGTTGAGC ACCATACGTTCTTTCAGGCAAAGAAGCTACTACCTCAGGAAAAGAATGTTCTGTCTCAGTACTGGACTAT GGGCTCTCGGAACACCAAAAAGCGAAGTCCTCGGCTCCGGCACGAAATCCGAAAGCCACGCCACTCTTCT GCAGATAACCTTGCAAATGAAATGACCTACATCACGGAAACGGAAGATGTATTTTACACGTACAAGGGCT CTCTGGCCCCTCAAGACAGCGATTCTGAAGTTTCTCAGAACCGAAGCCCGCACCAAGAGAGTTTATCCGA GAACAATCCGGCACAAAGCTACCTGACCCAGAAGTCATCCAGTTCTGTGTCTCCATCTTCAAATGCTCCA GGCTCCTGCTCACCTGACGGCGTTGATCAGCAGCTCTTAGATGACTTCCACAGGGTGACCAAAGGGGGCT CCACCGAGGACGCCAGCCAGTACTACTGTGACAAGAATGATAATGGTGACAGCTACTTAGTCTTGATCCG TATCACACCAGATGAAGATGGAAAATTTGGATTTAATCTTAAGGGAGGAGTGGATCAAAAGATGCCTCTT GTGGTATCAAGGATAAACCCAGAGTCACCTGCGGACACCTGCATTCCTAAGCTGAACGAAGGGGATCAAA TCGTGTTAATCAATGGCCGGGACATCTCAGAACACACGCATGACCAAGTGGTGATGTTCATCAAAGCCAG CCGGGAGTCCCACTCACGGGAGCTGGCCCTGGTGATCAGGAGGAGAGCTGTCCGCTCATTTGCTGACTTC AAGTCTGAAGATGAACTGAACCAGCTTTTCCCCGAAGCCATTTTCCCCATGTGTCCGGAGGGTGGGGACA CTTTGGAGGGATCCATGGCACAGCTAAAGAAGGGCCTCGAAAGCGGGACGGTGCTGATCCAGTTTGAGCA ACTCTACAGAAAAAAGCCAGGTTTGGCCATCACGTTTGCAAAGCTGCCTCAAAATTTGGACAAAAACCGA TATAAAGATGTGCTGCCTTATGACACCACCCGGGTATTATTGCAGGGAAATGAAGATTATATTAATGCAA GTTACGTGAACATGGAAATTCCTGCTGCTAACCTTGTGAACAAGTACATCGCCACTCAGGGGCCCCTGCC GCATACCTGTGCACAGTTTTGGCAGGTTGTCTGGGATCAGAAGTTGTCACTCATTGTCATGTTGACGACT CTCACAGAACGAGGGCGGACCAAATGTCACCAGTACTGGCCAGATCCCCCCGACGTCATGAACCACGGCG GCTTTCACATCCAGTGTCAGTCAGAGGACTGCACCATCGCCTATGTGTCCCGAGAAATGCTGGTCACAAA CACCCAGACCGGGGAAGAACACACAGTGACACATCTCCAGTACGTCGCATGGCCTGACCACGGTGTGCCC GATGACTCCTCCGACTTTCTGGAATTTGTAAACTATGTGAGGTCTCTGAGAGTGGACAGCGAGCCCGTCC TAGTTCACTGCAGTGCTGGAATAGGTCGAACCGGTGTGTTGGTCACTATGGAAACAGCCATGTGCCTAAC TGAGAGGAACCTGCCCATTTACCCACTGGATATTGTCCGAAAAATGCGAGACCAGCGCGCCATGATGGTG CAGACATCAAGCCAGTACAAGTTTGTGTGTGAAGCGATTCTTCGTGTGTATGAAGAAGGTTTAGTCCAAA TGCTGGATCCTAGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226819 representing NM_001145370
Red=Cloning site Green=Tags(s) MTSRLRALGGRINNIRTSELPKEKTRSEVICSIHFLDGVVQTFKVTKQDTGQVLLDMVHNHLGVTEKEYF GLQHDDDSVDSPRWLEASKAIRKQLKGGFPCTLHFRVRFFIPDPNTLQQEQTRHLYFLQLKMDICEGRLT CPLNSAVVLASYAVQSHFGDYNSSIHHPGYLSDSHFIPDQNEDFLTKVESLHEQHSGLKQSEAESCYINI ARTLDFYGVELHSGRDLHNLDLMIGIASAGVAVYRKYICTSFYPWVNILKISFKRKKFFIHQRQKQAESR EHIVAFNMLNYRSCKNLWKSCVEHHTFFQAKKLLPQEKNVLSQYWTMGSRNTKKRSPRLRHEIRKPRHSS ADNLANEMTYITETEDVFYTYKGSLAPQDSDSEVSQNRSPHQESLSENNPAQSYLTQKSSSSVSPSSNAP GSCSPDGVDQQLLDDFHRVTKGGSTEDASQYYCDKNDNGDSYLVLIRITPDEDGKFGFNLKGGVDQKMPL VVSRINPESPADTCIPKLNEGDQIVLINGRDISEHTHDQVVMFIKASRESHSRELALVIRRRAVRSFADF KSEDELNQLFPEAIFPMCPEGGDTLEGSMAQLKKGLESGTVLIQFEQLYRKKPGLAITFAKLPQNLDKNR YKDVLPYDTTRVLLQGNEDYINASYVNMEIPAANLVNKYIATQGPLPHTCAQFWQVVWDQKLSLIVMLTT LTERGRTKCHQYWPDPPDVMNHGGFHIQCQSEDCTIAYVSREMLVTNTQTGEEHTVTHLQYVAWPDHGVP DDSSDFLEFVNYVRSLRVDSEPVLVHCSAGIGRTGVLVTMETAMCLTERNLPIYPLDIVRKMRDQRAMMV QTSSQYKFVCEAILRVYEEGLVQMLDPS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001145370 |
| ORF Size | 2607 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001145370.1, NP_001138842.1 |
| RefSeq Size | 8648 bp |
| RefSeq ORF | 2214 bp |
| Locus ID | 5774 |
| UniProt ID | P26045 |
| Protein Families | Druggable Genome, Phosphatase |
| Gene Summary | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This protein contains a C-terminal PTP domain and an N-terminal domain homologous to the band 4.1 superfamily of cytoskeletal-associated proteins. P97, a cell cycle regulator involved in a variety of membrane related functions, has been shown to be a substrate of this PTP. This PTP was also found to interact with, and be regulated by adaptor protein 14-3-3 beta. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226819 | PTPN3 (Myc-DDK-tagged)-Human protein tyrosine phosphatase, non-receptor type 3 (PTPN3), transcript variant 4 |
CNY 5,800.00 |
|
| RC226819L3 | Lenti-ORF clone of PTPN3 (Myc-DDK-tagged)-Human protein tyrosine phosphatase, non-receptor type 3 (PTPN3), transcript variant 4 |
CNY 7,700.00 |
|
| RC226819L4 | Lenti-ORF clone of PTPN3 (mGFP-tagged)-Human protein tyrosine phosphatase, non-receptor type 3 (PTPN3), transcript variant 4 |
CNY 7,700.00 |
|
| SC325311 | PTPN3 (untagged)-Human protein tyrosine phosphatase, non-receptor type 3 (PTPN3), transcript variant 4 |
CNY 7,220.00 |
