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RPS6KC1 (NM_001136138) Human Tagged ORF Clone
CAT＃: RG226547
- TrueORF®
RPS6KC1 (tGFP-tagged) - Human ribosomal protein S6 kinase, 52kDa, polypeptide 1 (RPS6KC1), transcript variant 2
ORF Plasmid: DDK
"NM_001136138" in other vectors (4)
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CNY 11,590.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | humS6PKh1; RPK118; RSKL1; S6K-delta-1; S6PKh1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG226547 representing NM_001136138
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCTCTTACCGGGAGCGGAGTGCCGACCTGGCCCGTTTCTACACTGTCACCGAGCCCCAGCGACACC CGAGGGGCTACACAGTATATAAGGTCACCGCCCGGATAATTGTATGGAAGAGATACAGTGATTTTAAGAA ACTACACAAAGAACTATGGCAAATTCACAAAAACTTATTCCGACATTCAGAGTTGTTTCCTCCATTTGCT AAAGGAATAGTGTTTGGGCGATTTGATGAAACTGTTATCGAAGAGAGAAGACAATGTGCTGAAGACCTGC TACAGTTCTCTGCCAATATTCCTGCTCTTTACAATAGTAAACAGCTTGAAGACTTTTTCAAGGGTGGAAT AATTAATGATAGTTCTGAATTAATTGGTCCTGCTGAAGCTCACTCAGATTCCCTCATTGATACCTTTCCT GAGTGTAGTACGGAAGGCTTCTCCAGTGACAGTGATCTGGTATCTCTTACTGTTGATGTGGATTCTCTTG CTGAGTTAGATGATGGAATGGCTTCCAATCAAAATTCTCCCATTAGAACTTTTGGTCTCAATCTTTCTTC GGATTCTTCAGCACTAGGGGCTGTTGCTTCTGACAGTGAACAGAGCAAAACAGAAGAAGAACGGGAAAGT CGTAGCCTCTTTCCTGGCAGTTTAAAGCCGAAGCTTGGCAAGAGAGATTATTTGGAGAAAGCAGGAGAAT TAATAAAGCTGGCTTTAAAAAAGGAAGAAGAAGACGACTATGAAGCTGCTTCTGATTTTTATAGGAAGGG AGTTGATTTACTCCTAGAAGGTGTTCAAGGAGAGTCAAGCCCTACCCGTCGAGAAGCTGTGAAGAGAAGA ACAGCCGAGTACCTCATGCGGGCAGAAAGTATCTCTAGTCTTTATGGGAAACCTCAGCTTGATGATGTAT CTCAGCCTCCAGGATCACTAAGTTCAAGGCCCCTTTGGAACCTAAGGAGCCCTGCCGAGGAGCTGAAGGC CTTCAGAGTCCTTGGGGTGATTGACAAGGTTTTACTTGTAATGGACACAAGGACAGAACAGACTTTCATT TTAAAAGGTCTAAGGAAAAGCAGTGAATACAGCAGGAACAGAAAGACCATCATCCCCCGCTGTGTGCCCA ACATGGTGTGTCTGCATAAGTACATCATCTCTGAGGAGTCAGTATTTCTTGTGCTGCAGCATGCGGAAGG TGGCAAACTGTGGTCATATATCAGTAAATTTCTAAACAGAAGTCCTGAAGAAAGCTTTGACATCAAGGAA GTGAAAAAACCTACACTTGCAAAAGTTCACCTGCAGCAGCCAACTTCTAGTCCTCAGGACAGCAGTAGCT TTGAATCCAGAGGAAGTGATGGTGGAAGCATGCTTAAAGCTCTGCCTTTGAAGAGTAGTCTTACTCCAAG TTCTCAAGATGACAGCAACCAGGAAGATGATGGCCAAGATAGCTCTCCAAAGTGGCCAGATTCTGGTTCA AGTTCAGAAGAAGAATGTACTACTAGTTATTTAACATTATGCAATGAATATGGGCAAGAAAAGATTGAAC CAGGGTCTTTGAATGAGGAGCCCTTCATGAAGACTGAAGGGAATGGTGTTGATACAAAAGCTATTAAAAG CTTCCCAGCACACCTTGCTGCTGACAGTGACAGCCCCAGCACACAGCTGAGAGCTCACGAGCTGAAGTTC TTCCCCAACGATGACCCAGAAGCAGTTAGTTCTCCAAGAACATCAGATTCCCTCAGTAGATCAAAAAATA GCCCCATGGAATTCTTTAGGATAGACAGTAAGGATAGCGCAAGTGAACTCCTGGGACTTGACTTTGGAGA AAAATTGTATAGTCTAAAATCAGAACCTTTGAAACCATTCTTTACTCTTCCAGATGGAGACAGTGCTTCT AGGAGTTTTAATACTAGTGAAAGCAAGGTAGAGTTTAAAGCTCAGGACACCATTAGCAGGGGCTCAGATG ACTCAGTGCCAGTTATTTCATTTAAAGATGCTGCTTTTGATGATGTCAGTGGTACTGATGAAGGAAGACC TGATCTTCTTGTAAATTTACCTGGTGAATTGGAGTCAACAAGAGAAGCTGCAGCAATGGGACCTACTAAG TTTACACAAACTAATATAGGGATAATAGAAAATAAACTCTTGGAAGCCCCTGATGTTTTATGCCTCAGGC TTAGTACTGAACAATGCCAAGCACATGAGGAGAAAGGCATAGAGGAACTGAGTGATCCCTCTGGGCCCAA ATCCTATAGTATAACAGAGAAACACTATGCACAGGAGGATCCCAGGATGTTATTTGTAGCAGCTGTTGAT CATAGTAGTTCAGGAGATATGTCTTTGTTACCCAGCTCAGATCCTAAGTTTCAAGGACTTGGAGTGGTTG AGTCAGCAGTAACTGCAAACAACACAGAAGAAAGCTTATTCCGTATTTGTAGTCCACTCTCAGGTGCTAA TGAATATATTGCAAGCACAGACACTTTAAAAACAGAAGAAGTATTGCTGTTTACAGATCAGACTGATGAT TTGGCTAAAGAGGAACCAACTTCTTTATTCCAGAGAGACTCTGAGACTAAGGGTGAAAGTGGTTTAGTGC TAGAAGGAGACAAGGAAATACATCAGATTTTTGAGGACCTTGATAAAAAATTAGCACTAGCCTCCAGGTT TTACATCCCAGAGGGCTGCATTCAAAGATGGGCAGCTGAAATGGTGGTAGCCCTTGATGCTTTACATAGA GAGGGAATTGTGTGCCGCGATTTGAACCCAAACAACATCTTATTGAATGATAGAGGACACATTCAGCTAA CGTATTTTAGCAGGTGGAGTGAGGTTGAAGATTCCTGTGACAGCGATGCCATAGAGAGAATGTACTGTGC CCCAGAGGTTGGAGCAATCACTGAAGAAACTGAAGCCTGTGATTGGTGGAGTTTGGGTGCTGTCCTCTTT GAACTTCTCACTGGCAAGACTCTGGTTGAATGCCATCCAGCAGGAATAAATACTCACACTACTTTGAACA TGCCAGAATGTGTCTCTGAAGAGGCTCGCTCACTCATTCAACAGCTCTTGCAGTTCAATCCTCTGGAACG ACTTGGTGCTGGAGTTGCTGGTGTTGAAGATATCAAATCTCATCCATTTTTTACCCCTGTGGATTGGGCA GAACTGATGAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226547 representing NM_001136138
Red=Cloning site Green=Tags(s) MTSYRERSADLARFYTVTEPQRHPRGYTVYKVTARIIVWKRYSDFKKLHKELWQIHKNLFRHSELFPPFA KGIVFGRFDETVIEERRQCAEDLLQFSANIPALYNSKQLEDFFKGGIINDSSELIGPAEAHSDSLIDTFP ECSTEGFSSDSDLVSLTVDVDSLAELDDGMASNQNSPIRTFGLNLSSDSSALGAVASDSEQSKTEEERES RSLFPGSLKPKLGKRDYLEKAGELIKLALKKEEEDDYEAASDFYRKGVDLLLEGVQGESSPTRREAVKRR TAEYLMRAESISSLYGKPQLDDVSQPPGSLSSRPLWNLRSPAEELKAFRVLGVIDKVLLVMDTRTEQTFI LKGLRKSSEYSRNRKTIIPRCVPNMVCLHKYIISEESVFLVLQHAEGGKLWSYISKFLNRSPEESFDIKE VKKPTLAKVHLQQPTSSPQDSSSFESRGSDGGSMLKALPLKSSLTPSSQDDSNQEDDGQDSSPKWPDSGS SSEEECTTSYLTLCNEYGQEKIEPGSLNEEPFMKTEGNGVDTKAIKSFPAHLAADSDSPSTQLRAHELKF FPNDDPEAVSSPRTSDSLSRSKNSPMEFFRIDSKDSASELLGLDFGEKLYSLKSEPLKPFFTLPDGDSAS RSFNTSESKVEFKAQDTISRGSDDSVPVISFKDAAFDDVSGTDEGRPDLLVNLPGELESTREAAAMGPTK FTQTNIGIIENKLLEAPDVLCLRLSTEQCQAHEEKGIEELSDPSGPKSYSITEKHYAQEDPRMLFVAAVD HSSSGDMSLLPSSDPKFQGLGVVESAVTANNTEESLFRICSPLSGANEYIASTDTLKTEEVLLFTDQTDD LAKEEPTSLFQRDSETKGESGLVLEGDKEIHQIFEDLDKKLALASRFYIPEGCIQRWAAEMVVALDALHR EGIVCRDLNPNNILLNDRGHIQLTYFSRWSEVEDSCDSDAIERMYCAPEVGAITEETEACDWWSLGAVLF ELLTGKTLVECHPAGINTHTTLNMPECVSEEARSLIQQLLQFNPLERLGAGVAGVEDIKSHPFFTPVDWA ELMR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001136138 |
| ORF Size | 3162 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001136138.3 |
| RefSeq Size | 4170 bp |
| RefSeq ORF | 3165 bp |
| Locus ID | 26750 |
| UniProt ID | Q96S38 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | Sphingosine kinase catalyzes the formation of sphingosine 1 phosphate, a lipid cellular messenger. The protein encoded by this gene can bind to sphingosine kinase and to phosphatidylinositol 3-phosphate, suggesting a role in sphingosine 1 phophate signaling. The encoded protein can also bind to peroxiredoxin-3 and may help transport it to mitochondria. [provided by RefSeq, Mar 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226547 | RPS6KC1 (Myc-DDK-tagged)-Human ribosomal protein S6 kinase, 52kDa, polypeptide 1 (RPS6KC1), transcript variant 2 |
CNY 10,550.00 |
|
| RC226547L3 | Lenti-ORF clone of RPS6KC1 (Myc-DDK-tagged)-Human ribosomal protein S6 kinase, 52kDa, polypeptide 1 (RPS6KC1), transcript variant 2 |
CNY 12,450.00 |
|
| RC226547L4 | Lenti-ORF clone of RPS6KC1 (mGFP-tagged)-Human ribosomal protein S6 kinase, 52kDa, polypeptide 1 (RPS6KC1), transcript variant 2 |
CNY 12,450.00 |
|
| SC326323 | RPS6KC1 (untagged)-Human ribosomal protein S6 kinase, 52kDa, polypeptide 1 (RPS6KC1), transcript variant 2 |
CNY 20,330.00 |
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