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Dysferlin (DYSF) (NM_001130978) Human Tagged ORF Clone
CAT＃: RG226451
- TrueORF®
DYSF (tGFP-tagged) - Human dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant 11
ORF Plasmid: DDK
"NM_001130978" in other vectors (2)
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CNY 16,856.00
货期*
现货
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | FER1L1; LGMD2B; LGMDR2; MMD1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG226451 representing NM_001130978
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGAGGGTCTTCATCCTCTATGCCGAGAACGTCCACACACCCGACACCGACATCAGCGATGCCTACT GCTCCGCGGTGTTTGCAGGGGTGAAGAAGAGAACCAAAGTCATCAAGAACAGCGTGAACCCTGTATGGAA TGAGGGATTTGAATGGGACCTCAAGGGCATCCCCCTGGACCAGGGCTCTGAGCTTCATGTGGTGGTCAAA GACCATGAGACGATGGGGAGGAACAGGTTCCTGGGGGAAGCCAAGGTCCCACTCCGAGAGGTCCTCGCCA CCCCTAGTCTGTCCGCCAGCTTCAATGCCCCCCTGCTGGACACCAAGAAGCAGCCCACAGGGGCCTCGCT GGTCCTGCAGGTGTCCTACACACCGCTGCCTGGAGCTGTGCCCCTGTTCCCGCCCCCTACTCCTCTGGAG CCCTCCCCGACTCTGCCTGACCTGGATGTAGTGGCAGACACAGGAGGAGAGGAAGACACAGAGGACCAGG GACTCACTGGAGATGAGGCGGAGCCATTCCTGGATCAAAGCGGAGGCCCGGGGGCTCCCACCACCCCAAG GAAACTACCTTCACGTCCTCCGCCCCACTACCCCGGGATCAAAAGAAAGCGAAGTGCGCCTACATCTAGA AAGCTGCTGTCAGACAAACCGCAGGATTTCCAGATCAGGGTCCAGGTGATCGAGGGGCGCCAGCTGCCGG GGGTGAACATCAAGCCTGTGGTCAAGGTTACCGCTGCAGGGCAGACCAAGCGGACGCGGATCCACAAGGG AAACAGCCCACTCTTCAATGAGACTCTTTTCTTCAACTTGTTTGACTCTCCTGGGGAGCTGTTTGATGAG CCCATCTTTATCACGGTGGTAGACTCTCGTTCTCTCAGGACAGATGCTCTCCTCGGGGAGTTCCGGATGG ACGTGGGCACCATTTACAGAGAGCCCCGGCACGCCTATCTCAGGAAGTGGCTGCTGCTCTCAGACCCTGA TGACTTCTCTGCTGGGGCCAGAGGCTACCTGAAAACAAGCCTTTGTGTGCTGGGGCCTGGGGACGAAGCG CCTCTGGAGAGAAAAGACCCCTCTGAAGACAAGGAGGACATTGAAAGCAACCTGCTCCGGCCCACAGGCG TAGCCCTGCGAGGAGCCCACTTCTGCCTGAAGGTCTTCCGGGCCGAGGACTTGCCGCAGATGGACGATGC CGTGATGGACAACGTGAAACAGATCTTTGGCTTCGAGAGTAACAAGAAGAACTTGGTGGACCCCTTTGTG GAGGTCAGCTTTGCGGGGAAAATGCTGTGCAGCAAGATCTTGGAGAAGACGGCCAACCCTCAGTGGAACC AGAACATCACACTGCCTGCCATGTTTCCCTCCATGTGCGAAAAAATGAGGATTCGTATCATAGACTGGGA CCGCCTGACTCACAATGACATCGTGGCTACCACCTACCTGAGTATGTCGAAAATCTCTGCCCCTGGAGGA GAAATAGAAGAGGAGCCTGCAGGTGCTGTCAAGCCTTCGAAAGCCTCAGACTTGGATGACTACCTGGGCT TCCTCCCCACTTTTGGGCCCTGCTACATCAACCTCTATGGCAGTCCCAGAGAGTTCACAGGCTTCCCAGA CCCCTACACAGAGCTCAACACAGGCAAGGGGGAAGGTGTGGCTTATCGTGGCCGGCTTCTGCTCTCCCTG GAGACCAAGCTGGTGGAGCACAGTGAACAGAAGGTGGAGGACCTTCCTGCGGATGACATCCTCCGGGTGG AGAAGTACCTTAGGAGGCGCAAGTACTCCCTGTTTGCGGCCTTCTACTCAGCCACCATGCTGCAGGATGT GGATGATGCCATCCAGTTTGAGGTCAGCATCGGGAACTACGGGAACAAGTTCGACATGACCTGCCTGCCG CTGGCCTCCACCACTCAGTACAGCCGTGCAGTCTTTGACGGGTGCCACTACTACTACCTACCCTGGGGTA ACGTGAAACCTGTGGTGGTGCTGTCATCCTACTGGGAGGACATCAGCCATAGAATCGAGACTCAGAACCA GCTGCTTGGGATTGCTGACCGGCTGGAAGCTGGCCTGGAGCAGGTCCACCTGGCCCTGAAGGCGCAGTGC TCCACGGAGGACGTGGACTCGCTGGTGGCTCAGCTGACGGATGAGCTCATCGCAGGCTGCAGCCAGCCTC TGGGTGACATCCATGAGACACCCTCTGCCACCCACCTGGACCAGTACCTGTACCAGCTGCGCACCCATCA CCTGAGCCAAATCACTGAGGCTGCCCTGGCCCTGAAGCTCGGCCACAGTGAGCTCCCTGCAGCTCTGGAG CAGGCGGAGGACTGGCTCCTGCGTCTGCGTGCCCTGGCAGAGGAGCCCCAGAACAGCCTGCCGGACATCG TCATCTGGATGCTGCAGGGAGACAAGCGTGTGGCATACCAGCGGGTGCCCGCCCACCAAGTCCTCTTCTC CCGGCGGGGTGCCAACTACTGTGGCAAGAATTGTGGGAAGCTACAGACAATCTTTCTGAAATATCCGATG GAGAAGGTGCCTGGCGCCCGGATGCCAGTGCAGATACGGGTCAAGCTGTGGTTTGGGCTCTCAGTGGATG AGAAGGAGTTCAACCAGTTTGCTGAGGGGAAGCTGTCTGTCTTTGCTGAAACCTATGAGAACGAGACTAA GTTGGCCCTTGTTGGGAACTGGGGCACAACGGGCCTCACCTACCCCAAGTTTTCTGACGTCACGGGCAAG ATCAAGCTACCCAAGGACAGCTTCCGCCCCTCGGCCGGCTGGACCTGGGCTGGAGATTGGTTCGTGTGTC CGGAGAAGACTCTGCTCCATGACATGGACGCCGGTCACCTGAGCTTCGTGGAAGAGGTGTTTGAGAACCA GACCCGGCTTCCCGGAGGCCAGTGGATCTACATGAGTGACAACTACACCGATGTGAACGGGGAGAAGGTG CTTCCCAAGGATGACATTGAGTGCCCACTGGGCTGGAAGTGGGAAGATGAGGAATGGTCCACAGACCTCA ACCGGGCTGTCGATGAGCAAGGCTGGGAGTATAGCATCACCATCCCCCCGGAGCGGAAGCCGAAGCACTG GGTCCCTGCTGAGAAGATGTACTACACACACCGACGGCGGCGCTGGGTGCGCCTGCGCAGGAGGGATCTC AGCCAAATGGAAGCACTGAAAAGGCACAGGCAGGCGGAGGCGGAGGGCGAGGGCTGGGAGTACGCCTCTC TTTTTGGCTGGAAGTTCCACCTCGAGTACCGCAAGACAGATGCCTTCCGCCGCCGCCGCTGGCGCCGTCG CATGGAGCCACTGGAGAAGACGGGGCCTGCAGCTGTGTTTGCCCTTGAGGGGGCCCTGGGCGGCGTGATG GATGACAAGAGTGAAGATTCCATGTCCGTCTCCACCTTGAGCTTCGGTGTGAACAGACCCACGATTTCCT GCATATTCGACTATGGGAACCGCTACCATCTACGCTGCTACATGTACCAGGCCCGGGACCTGGCTGCGAT GGACAAGGACTCTTTTTCTGATCCCTATGCCATCGTCTCCTTCCTGCACCAGAGCCAGAAGACGGTGGTG GTGAAGAACACCCTTAACCCCACCTGGGACCAGACGCTCATCTTCTACGAGATCGAGATCTTTGGCGAGC CGGCCACAGTTGCTGAGCAACCGCCCAGCATTGTGGTGGAGCTGTACGACCATGACACTTATGGTGCAGA CGAGTTTATGGGTCGCTGCATCTGTCAACCGAGTCTGGAACGGATGCCACGGCTGGCCTGGTTCCCACTG ACGAGGGGCAGCCAGCCGTCGGGGGAGCTGCTGGCCTCTTTTGAGCTCATCCAGAGAGAGAAGCCGGCCA TCCACCATATTCCTGGTTTTGAGGTGCAGGAGACATCAAGGATCCTGGATGAGTCTGAGGACACAGACCT GCCCTACCCACCACCCCAGAGGGAGGCCAACATCTACATGGTTCCTCAGAACATCAAGCCAGCGCTCCAG CGTACCGCCATCGAGATCCTGGCATGGGGCCTGCGGAACATGAAGAGTTACCAGCTGGCCAACATCTCCT CCCCCAGCCTCGTGGTAGAGTGTGGGGGCCAGACGGTGCAGTCCTGTGTCATCAGGAACCTCCGGAAGAA CCCCAACTTTGACATCTGCACCCTCTTCATGGAAGTGATGCTGCCCAGGGAGGAGCTCTACTGCCCCCCC ATCACCGTCAAGGTCATCGATAACCGCCAGTTTGGCCGCCGGCCTGTGGTGGGCCAGTGTACCATCCGCT CCCTGGAGAGCTTCCTGTGTGACCCCTACTCGGCGGAGAGTCCATCCCCACAGGGTGGCCCAGACGATGT GAGCCTACTCAGTCCTGGGGAAGACGTGCTCATCGACATTGATGACAAGGAGCCCCTCATCCCCATCCAG CTTGCAGACGGTCTGTCGAGCTTGGCCCCCACTAACACGGCTTCTCCTCCATCCAGTCCTCATGAGGAAG AGTTCATCGATTGGTGGAGCAAATTCTTTGCCTCCATAGGGGAGAGGGAAAAGTGCGGCTCCTACCTGGA GAAGGATTTTGACACCCTGAAGGTCTATGACACACAGCTGGAGAATGTGGAGGCCTTTGAGGGCCTGTCT GACTTTTGTAACACCTTCAAGCTGTACCGGGGCAAGACGCAGGAGGAGACAGAAGATCCATCTGTGATTG GTGAATTTAAGGGCCTCTTCAAAATTTATCCCCTCCCAGAAGACCCAGCCATCCCCATGCCCCCAAGACA GTTCCACCAGCTGGCCGCCCAGGGACCCCAGGAGTGCTTGGTCCGTATCTACATTGTCCGAGCATTTGGC CTGCAGCCCAAGGACCCCAATGGAAAGTGTGATCCTTACATCAAGATCTCCATAGGGAAGAAATCAGTGA GTGACCAGGATAACTACATCCCCTGCACGCTGGAGCCCGTATTTGGAAAGATGTTCGAGCTGACCTGCAC TCTGCCTCTGGAGAAGGACCTAAAGATCACTCTCTATGACTATGACCTCCTCTCCAAGGACGAAAAGATC GGTGAGACGGTCGTCGACCTGGAGAACAGGCTGCTGTCCAAGTTTGGGGCTCGCTGTGGACTCCCACAGA CCTACTGTGTCTCTGGACCGAACCAGTGGCGGGACCAGCTCCGCCCCTCCCAGCTCCTCCACCTCTTCTG CCAGCAGCATAGAGTCAAGGCACCTGTGTACCGGACAGACCGTGTAATGTTTCAGGATAAAGAATATTCC ATTGAAGAGATAGAGGCTGGCAGGATCCCAAACCCACACCTGGGCCCAGTGGAGGAGCGTCTGGCTCTGC ATGTGCTTCAGCAGCAGGGCCTGGTCCCGGAGCACGTGGAGTCACGGCCCCTCTACAGCCCCCTGCAGCC AGACATCGAGCAGGGGAAGCTGCAGATGTGGGTCGACCTATTTCCGAAGGCCCTGGGGCGGCCTGGACCT CCCTTCAACATCACCCCACGGAGAGCCAGAAGGTTTTTCCTGCGTTGTATTATCTGGAATACCAGAGATG TGATCCTGGATGACCTGAGCCTCACGGGGGAGAAGATGAGCGACATTTATGTGAAAGGTTGGATGATTGG CTTTGAAGAACACAAGCAAAAGACAGACGTGCATTATCGTTCCCTGGGAGGTGAAGGCAACTTCAACTGG AGGTTCATTTTCCCCTTCGACTACCTGCCAGCTGAGCAAGTCTGTACCATTGCCAAGAAGGATGCCTTCT GGAGGCTGGACAAGACTGAGAGCAAAATCCCAGCACGAGTGGTGTTCCAGATCTGGGACAATGACAAGTT CTCCTTTGATGATTTTCTGGGCTCCCTGCAGCTCGATCTCAACCGCATGCCCAAGCCAGCCAAGACAGCC AAGAAGTGCTCCTTGGACCAGCTGGATGATGCTTTCCACCCAGAATGGTTTGTGTCCCTTTTTGAGCAGA AAACAGTGAAGGGCTGGTGGCCCTGTGTAGCAGAAGAGGGTGAGAAGAAAATACTGGCGGGCAAGCTGGA AATGACCTTGGAGATTGTAGCAGAGAGTGAGCATGAGGAGCGGCCTGCTGGCCAGGGCCGGGATGAGCCC AACATGAACCCTAAGCTTGAGGACCCAAGGCGCCCCGACACCTCCTTCCTGTGGTTTACCTCCCCATACA AGACCATGAAGTTCATCCTGTGGCGGCGTTTCCGGTGGGCCATCATCCTCTTCATCATCCTCTTCATCCT GCTGCTGTTCCTGGCCATCTTCATCTACGCCTTCCCGAACTATGCTGCCATGAAGCTGGTGAAGCCCTTC AGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226451 representing NM_001130978
Red=Cloning site Green=Tags(s) MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVK DHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLE PSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSR KLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDE PIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEA PLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFV EVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGG EIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSL ETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLP LASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQC STEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALE QAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPM EKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGK IKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKV LPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDL SQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVM DDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVV VKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPL TRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQ RTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPP ITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQ LADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLS DFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFG LQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKI GETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYS IEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGP PFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNW RFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTA KKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEP NMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPF S TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001130978 |
| ORF Size | 6303 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001130978.2 |
| RefSeq Size | 6953 bp |
| RefSeq ORF | 6306 bp |
| Locus ID | 8291 |
| UniProt ID | O75923 |
| Protein Families | Transmembrane |
| Gene Summary | The protein encoded by this gene belongs to the ferlin family and is a skeletal muscle protein found associated with the sarcolemma. It is involved in muscle contraction and contains C2 domains that play a role in calcium-mediated membrane fusion events, suggesting that it may be involved in membrane regeneration and repair. In addition, the protein encoded by this gene binds caveolin-3, a skeletal muscle membrane protein which is important in the formation of caveolae. Specific mutations in this gene have been shown to cause autosomal recessive limb girdle muscular dystrophy type 2B (LGMD2B) as well as Miyoshi myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226451 | DYSF (Myc-DDK-tagged)-Human dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant 11 |
CNY 18,990.00 |
|
| SC326431 | DYSF (untagged)-Human dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (DYSF), transcript variant 11 |
CNY 40,380.00 |
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