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PAG3 (ASAP2) (NM_001135191) Human Tagged ORF Clone
CAT＃: RG226279
- TrueORF®
ASAP2 (tGFP-tagged) - Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (ASAP2), transcript variant 2
ORF Plasmid: DDK
"NM_001135191" in other vectors (4)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha; SHAG1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG226279 representing NM_001135191
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGACCAGATCTCCGTGTCGGAATTCGTGGCCGAGACCCATGAGGACTACAAGGCGCCCACGGCCT CCAGCTTCACCACCCGCACGGCGCAGTGCCGGAACACTGTGGCGGCCATCGAGGAGGCTTTGGACGTGGA CCGGATGGTTCTTTACAAAATGAAGAAATCCGTGAAAGCAATCAACAGCTCTGGGCTGGCTCACGTGGAA AATGAAGAGCAGTACACCCAGGCTCTGGAGAAGTTTGGCGGCAACTGTGTATGCAGAGATGACCCAGATT TAGGAAGTGCGTTCCTGAAGTTCTCAGTGTTTACAAAGGAGTTGACAGCACTTTTCAAAAACCTGATTCA GAATATGAACAACATAATCTCCTTCCCTTTGGACAGTTTGCTGAAGGGGGACCTGAAAGGAGTGAAAGGG GATCTGAAAAAGCCTTTTGATAAAGCTTGGAAGGACTATGAAACAAAAATAACCAAGATAGAAAAGGAGA AAAAGGAACACGCCAAGCTCCATGGGATGATTCGGACTGAAATAAGCGGAGCGGAAATTGCCGAAGAGAT GGAAAAGGAGAGGCGCTTCTTCCAGCTACAGATGTGCGAGTATCTGCTGAAGGTCAACGAAATCAAGATT AAAAAGGGAGTAGATTTACTTCAGAATCTGATCAAATACTTTCATGCCCAATGCAATTTTTTTCAGGATG GACTCAAAGCCGTGGAAAGCCTCAAACCTTCCATTGAAACGCTGTCTACGGATCTTCACACGATCAAACA GGCCCAGGATGAAGAAAGAAGGCAGTTGATACAGCTTCGAGATATTTTGAAATCCGCATTGCAGGTTGAA CAGAAAGAGGACTCCCAAATTCGTCAGAGCACAGCTTATAGCTTACATCAGCCTCAGGGAAACAAGGAAC ATGGGACCGAGCGGAACGGCAGCCTCTACAAGAAGAGTGACGGGATCCGAAAAGTGTGGCAGAAAAGGAA ATGTTCAGTTAAAAATGGTTTTCTGACCATATCCCATGGTACCGCTAACCGGCCTCCTGCAAAGCTCAAC CTGCTAACCTGCCAGGTGAAGACCAACCCTGAGGAGAAGAAGTGCTTTGACCTCATTTCACATGACAGAA CTTACCACTTTCAAGCTGAAGATGAACAGGAATGTCAAATATGGATGTCTGTGCTGCAAAATAGCAAAGA AGAAGCTTTAAACAATGCATTTAAGGGGGATGACAATACTGGAGAAAATAACATCGTCCAAGAACTGACA AAGGAGATCATCTCAGAAGTGCAGAGGATGACGGGCAATGACGTCTGCTGTGACTGTGGGGCGCCAGATC CTACATGGCTTTCCACCAACCTGGGCATCCTGACCTGCATCGAGTGTTCCGGAATCCACCGAGAGCTGGG GGTTCATTATTCCAGGATGCAGTCCCTGACCTTAGATGTACTGGGAACATCTGAGCTGCTGCTCGCCAAG AATATTGGGAATGCAGGCTTTAATGAGATCATGGAATGTTGCCTACCAGCTGAGGACTCAGTCAAACCCA ACCCAGGCAGCGACATGAATGCAAGAAAGGACTACATCACAGCCAAGTACATCGAGAGGAGATACGCAAG GAAGAAGCACGCGGATAACGCGGCGAAGCTTCACAGTCTTTGCGAGGCCGTCAAAACGAGAGATATTTTT GGATTGCTCCAAGCTTATGCTGATGGTGTGGATCTTACGGAAAAAATCCCACTGGCCAACGGACATGAGC CGGATGAAACGGCCCTCCACCTTGCAGTCAGATCCGTGGATCGAACCTCTCTTCACATTGTAGACTTTTT AGTTCAGAACAGTGGGAACCTGGATAAACAGACAGGGAAAGGCAGCACAGCCCTGCACTACTGCTGCCTG ACCGACAATGCCGAGTGCCTCAAGTTGCTCCTGCGGGGGAAGGCCTCCATCGAGATAGCAAACGAGTCAG GAGAGACTCCGCTGGACATTGCCAAGCGCCTCAAGCACGAGCACTGTGAGGAGCTGCTGACCCAAGCCTT ATCTGGAAGATTTAATTCTCACGTTCACGTTGAATATGAATGGCGACTACTCCACGAAGACCTGGATGAA AGTGATGACGACATGGATGAGAAATTGCAGCCCAGTCCCAACCGGCGGGAAGACCGGCCCATCAGCTTCT ACCAGCTGGGCTCCAACCAGCTTCAGTCTAACGCTGTATCTTTGGCCAGAGATGCTGCAAACCTTGCCAA GGAGAAGCAGAGGGCTTTCATGCCCAGCATCTTGCAGAATGAGACTTACGGAGCCCTCCTGAGTGGCAGC CCACCTCCCGCCCAGCCTGCAGCCCCCAGCACCACCAGCGCCCCCCCGCTTCCTCCACGGAATGTTGGCA AAGATCCCCTGACCCCCACGCCGCCCCCACCCGTTGCCAAGACGCCCAGCGTAATGGAAGCCTTGAGCCA GCCGAGCAAGCCTGCCCCGCCTGGGATCTCACAGATCAGGCCCCCACCTCTGCCCCCACAGCCGCCCAGC CGCCTCCCGCAGAAGAAGCCTGCGCCGGGGGCTGACAAGTCCACCCCACTGACCAACAAAGGCCAACCGA GAGGACCTGTGGATCTCTCTGCAACGGAAGCTCTGGGTCCTCTGTCCAATGCTATGGTCCTGCAGCCCCC TGCACCCATGCCTAGGAAGTCGCAGGCAACCAAGTTGAAGCCTAAGCGGGTGAAAGCGCTCTATAACTGT GTGGCTGACAACCCCGATGAGCTCACCTTCTCCGAGGGGGATGTGATCATCGTGGACGGGGAGGAGGACC AGGAGTGGTGGATTGGCCACATTGATGGAGATCCTGGTCGCAAAGGCGCATTCCCGGTGTCATTTGTGCA CTTTATCGCTGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226279 representing NM_001135191
Red=Cloning site Green=Tags(s) MPDQISVSEFVAETHEDYKAPTASSFTTRTAQCRNTVAAIEEALDVDRMVLYKMKKSVKAINSSGLAHVE NEEQYTQALEKFGGNCVCRDDPDLGSAFLKFSVFTKELTALFKNLIQNMNNIISFPLDSLLKGDLKGVKG DLKKPFDKAWKDYETKITKIEKEKKEHAKLHGMIRTEISGAEIAEEMEKERRFFQLQMCEYLLKVNEIKI KKGVDLLQNLIKYFHAQCNFFQDGLKAVESLKPSIETLSTDLHTIKQAQDEERRQLIQLRDILKSALQVE QKEDSQIRQSTAYSLHQPQGNKEHGTERNGSLYKKSDGIRKVWQKRKCSVKNGFLTISHGTANRPPAKLN LLTCQVKTNPEEKKCFDLISHDRTYHFQAEDEQECQIWMSVLQNSKEEALNNAFKGDDNTGENNIVQELT KEIISEVQRMTGNDVCCDCGAPDPTWLSTNLGILTCIECSGIHRELGVHYSRMQSLTLDVLGTSELLLAK NIGNAGFNEIMECCLPAEDSVKPNPGSDMNARKDYITAKYIERRYARKKHADNAAKLHSLCEAVKTRDIF GLLQAYADGVDLTEKIPLANGHEPDETALHLAVRSVDRTSLHIVDFLVQNSGNLDKQTGKGSTALHYCCL TDNAECLKLLLRGKASIEIANESGETPLDIAKRLKHEHCEELLTQALSGRFNSHVHVEYEWRLLHEDLDE SDDDMDEKLQPSPNRREDRPISFYQLGSNQLQSNAVSLARDAANLAKEKQRAFMPSILQNETYGALLSGS PPPAQPAAPSTTSAPPLPPRNVGKDPLTPTPPPPVAKTPSVMEALSQPSKPAPPGISQIRPPPLPPQPPS RLPQKKPAPGADKSTPLTNKGQPRGPVDLSATEALGPLSNAMVLQPPAPMPRKSQATKLKPKRVKALYNC VADNPDELTFSEGDVIIVDGEEDQEWWIGHIDGDPGRKGAFPVSFVHFIAD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001135191 |
| ORF Size | 2883 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001135191.1, NP_001128663.1 |
| RefSeq Size | 5589 bp |
| RefSeq ORF | 2886 bp |
| Locus ID | 8853 |
| UniProt ID | O43150 |
| Protein Pathways | Endocytosis, Fc gamma R-mediated phagocytosis |
| Gene Summary | This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226279 | ASAP2 (Myc-DDK-tagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (ASAP2), transcript variant 2 |
CNY 7,130.00 |
|
| RC226279L3 | Lenti-ORF clone of ASAP2 (Myc-DDK-tagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (ASAP2), transcript variant 2 |
CNY 9,030.00 |
|
| RC226279L4 | Lenti-ORF clone of ASAP2 (mGFP-tagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (ASAP2), transcript variant 2 |
CNY 9,030.00 |
|
| SC325379 | ASAP2 (untagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (ASAP2), transcript variant 2 |
CNY 7,220.00 |
