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SPRED2 (NM_001128210) Human Tagged ORF Clone
CAT＃: RG225652
- TrueORF®
SPRED2 (tGFP-tagged) - Human sprouty-related, EVH1 domain containing 2 (SPRED2), transcript variant 2
ORF Plasmid: DDK
"NM_001128210" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Spred-2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG225652 representing NM_001128210
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATCGCCTGGCAGTGACAGCTATATTGTGCGTGTCAAGGCTGTGGTTATGACCAGAGATGACTCCA GCGGGGGATGGTTCCCACAGGAAGGAGGCGGGATCAGTCGCGTCGGGGTCTGTAAGGTCATGCACCCCGA AGGCAATGGACGAAGCGGCTTTCTCATCCATGGTGAACGACAGAAAGACAAACTGGTGGTATTGGAATGC TATGTAAGAAAGGACTTGGTCTACACCAAAGCCAATCCAACGTTTCATCACTGGAAGGTCGATAATAGGA AGTTTGGACTTACTTTCCAAAGCCCTGCTGATGCCCGAGCCTTTGACAGGGGAGTAAGGAAAGCAATCGA AGACCTTATAGAAGGTTCAACAACGTCATCTTCCACCATCCATAATGAAGCTGAGCTTGGCGATGATGAC GTTTTTACAACAGCTACAGACAGTTCTTCTAATTCCTCTCAGAAGAGAGAGCAACCTACTCGGACAATCT CCTCTCCCACATCCTGTGAGCACCGGAGGATTTATACCCTGGGCCACCTCCACGACTCATACCCCACAGA CCACTATCACCTCGATCAGCCGATGCCAAGGCCCTACCGCCAGGTGAGCTTCCCGGACGACGACGAGGAG ATCGTGCGCATCAACCCCCGGGAGAAGATCTGGATGACGGGGTACGAGGATTACCGGCACGCACCCGTCA GGGGCAAGTACCCGGACCCCTCGGAGGACGCGGACTCCTCCTACGTGCGCTTCGCCAAGGGCGAGGTCCC CAAGCATGACTACAACTACCCCTACGTGGACTCCTCAGACTTTGGCCTAGGCGAGGACCCCAAAGGCCGC GGGGGCAGCGTGATCAAGACGCAGCCCTCCCGGGGCAAGTCGCGGCGGCGGAAGGAGGACGGAGAGCGCT CGCGGTGCGTGTACTGCAGGGACATGTTCAACCACGAGGAGAACCGCCGGGGCCACTGCCAGGACGCGCC CGACTCCGTGAGAACTTGCATCCGCCGGGTGAGCTGCATGTGGTGCGCGGACAGCATGCTCTATCACTGT ATGTCGGACCCCGAGGGAGACTATACAGACCCTTGCTCGTGCGATACTAGCGACGAGAAGTTTTGCCTCC GGTGGATGGCTCTTATTGCCTTGTCTTTCCTGGCCCCCTGTATGTGCTGTTACCTGCCCCTTCGGGCCTG CTACCACTGCGGAGTGATGTGCAGGTGCTGTGGCGGGAAGCACAAAGCGGCCGCG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG225652 representing NM_001128210
Red=Cloning site Green=Tags(s) MASPGSDSYIVRVKAVVMTRDDSSGGWFPQEGGGISRVGVCKVMHPEGNGRSGFLIHGERQKDKLVVLEC YVRKDLVYTKANPTFHHWKVDNRKFGLTFQSPADARAFDRGVRKAIEDLIEGSTTSSSTIHNEAELGDDD VFTTATDSSSNSSQKREQPTRTISSPTSCEHRRIYTLGHLHDSYPTDHYHLDQPMPRPYRQVSFPDDDEE IVRINPREKIWMTGYEDYRHAPVRGKYPDPSEDADSSYVRFAKGEVPKHDYNYPYVDSSDFGLGEDPKGR GGSVIKTQPSRGKSRRRKEDGERSRCVYCRDMFNHEENRRGHCQDAPDSVRTCIRRVSCMWCADSMLYHC MSDPEGDYTDPCSCDTSDEKFCLRWMALIALSFLAPCMCCYLPLRACYHCGVMCRCCGGKHKAAA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001128210 |
| ORF Size | 1245 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001128210.2 |
| RefSeq Size | 4104 bp |
| RefSeq ORF | 1248 bp |
| Locus ID | 200734 |
| UniProt ID | Q7Z698 |
| Protein Pathways | Jak-STAT signaling pathway |
| Gene Summary | SPRED2 is a member of the Sprouty (see SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see MAPK1; MIM 176948) (Nonami et al., 2004 [PubMed 15465815]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC225652 | SPRED2 (Myc-DDK-tagged)-Human sprouty-related, EVH1 domain containing 2 (SPRED2), transcript variant 2 |
CNY 3,656.00 |
|
| RC225652L3 | Lenti-ORF clone of SPRED2 (Myc-DDK-tagged)-Human sprouty-related, EVH1 domain containing 2 (SPRED2), transcript variant 2 |
CNY 5,890.00 |
|
| RC225652L4 | Lenti-ORF clone of SPRED2 (mGFP-tagged)-Human sprouty-related, EVH1 domain containing 2 (SPRED2), transcript variant 2 |
CNY 5,890.00 |
|
| SC323030 | SPRED2 (untagged)-Human sprouty-related, EVH1 domain containing 2 (SPRED2), transcript variant 2 |
CNY 6,650.00 |
