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RABEP1 (NM_001083585) Human Tagged ORF Clone
CAT＃: RG224499
- TrueORF®
RABEP1 (tGFP-tagged) - Human rabaptin, RAB GTPase binding effector protein 1 (RABEP1), transcript variant 2
ORF Plasmid: DDK
"NM_001083585" in other vectors (4)
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CNY 9,500.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | RAB5EP; RABPT5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG224499 representing NM_001083585
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCAGCCGGGCCCGGCTTCCCAGCCTGACGTTTCTCTTCAGCAACGGGTAGCAGAATTGGAAAAAA TTAATGCAGAATTTTTACGTGCACAACAGCAGCTTGAACAAGAATTTAATCAAAAGAGAGCAAAATTTAA GGAGTTATATTTGGCTAAAGAGGAGGATCTGAAGAGGCAAAATGCAGTATTACAAGCTGCACAAGATGAT TTGGGACACCTTCGAACCCAGCTGTGGGAAGCTCAAGCAGAGATGGAGAATATTAAGGCGATTGCCACAG TCTCTGAGAACACCAAGCAAGAAGCTATAGATGAAGTGAAAAGACAGTGGAGAGAAGAAGTTGCTTCACT TCAGGCTGTTATGAAAGAAACAGTTCGTGACTATGAGCACCAGTTCCACCTTAGGCTGGAGCAGGAGCGA ACACAGTGGGCACAGTATAGAGAATCCGCAGAGAGGGAAATAGCTGATTTAAGAAGAAGGCTGTCTGAAG GTCAAGAGGAGGAAAATTTAGAAAATGAAATGAAAAAGGCCCAAGAGGATGCTGAGAAACTTCGGTCCGT TGTGATGCCAATGGAAAAGGAAATTGCAGCTTTGAAGGATAAACTGACAGAGGCTGAAGACAAAATTAAA GAGCTGGAGGCCTCAAAGGTTAAAGAACTGAATCATTATCTGGAAGCTGAGAAATCTTGTAGGACTGATC TAGAGATGTATGTAGCTGTTTTGAATACTCAGAAATCTGTTCTACAGGAAGATGCTGAGAAACTGCGGAA AGAATTGCATGAAGTTTGCCATCTCTTGGAGCAAGAGCGACAACAACACAACCAGTTAAAACATACGTGG CAGAAGGCCAATGACCAGTTTCTGGAATCTCAGCGTTTACTGATGAGAGACATGCAGCGAATGGAGATTG TGCTAACTTCAGAACAGCTCCGACAAGTTGAAGAACTGAAGAAGAAAGATCAGGAGGATGATGAACAACA AAGACTCAATAAGAGAAAGGATCACAAAAAAGCAGATGTTGAGGAAGAAATAAAAATACCAGTAGTGTGT GCTTTAACTCAAGAAGAATCTTCAGCCCAGTTATCAAATGAAGAGGAGCATTTAGACAGCACCCGTGGCT CAGTTCATTCCTTAGATGCAGGCTTGCTGTTGCCATCTGGAGATCCTTTCAGTAAATCGGACAATGACAT GTTTAAAGATGGACTCAGGAGAGCACAGTCTACAGACAGCTTGGGAACCTCGGGCTCATTGCAATCCAAA GCTTTAGGCTATAACTACAAAGCAAAATCTGCTGGAAACCTGGACGAGTCAGATTTTGGACCACTGGTAG GAGCAGATTCAGTGTCTGAGAACTTTGATACTGCATCCCTTGGGTCACTCCAGATGCCAAGTGGGTTTAT GTTAACCAAAGATCAGGAAAGAGCAATCAAGGCGATGACACCAGAACAAGAAGAGACAGCGTCCCTCCTC TCCAGCGTTACCCAGGGCATGGAGAGTGCCTATGTGTCCCCTAGTGGTTATCGTTTAGTTAGTGAAACAG AATGGAATCTCTTGCAGAAAGAGGTACATAATGCTGGAAATAAACTTGGTAGACGTTGTGATATGTGTTC CAATTACGAAAAACAGTTACAAGGAATTCAGATTCAGGAGGCTGAAACGAGAGACCAGGTGAAAAAACTA CAGCTGATGCTAAGGCAAGCTAATGACCAGTTAGAGAAGACAATGAAAGATAAGCAGGAGCTGGAAGACT TCATAAAGCAAAGCAGCGAAGATTCGAGTCACCAGATCTCTGCACTCGTCCTAAGAGCCCAGGCCTCCGA GATCTTACTTGAAGAGTTACAGCAGGGGCTTTCCCAGGCAAAGAGGGATGTTCAGGAACAGATGGCGGTG CTGATGCAGTCACGGGAACAGGTTTCAGAAGAGCTGGTGAGGTTACAGAAAGATAATGACAGTCTCCAGG GAAAGCACAGCCTGCATGTGTCATTACAGCAAGCAGAAGACTTCATCCTCCCAGACACTACAGAGGCACT GCGGGAGTTGGTATTAAAATACCGTGAGGACATCATTAATGTGCGGACAGCAGCAGACCACGTAGAAGAA AAGCTGAAGGCTGAGATACTTTTCCTAAAAGAGCAGATCCAAGCAGAACAGTGTTTAAAAGAAAATCTTG AAGAAACTCTGCAACTAGAAATAGAAAACTGCAAGGAGGAAATAGCTTCTATTTCTAGCCTAAAAGCTGA ATTAGAAAGAATAAAAGTGGAAAAAGGACAGGCTACCGTTGAACAACTAATGTTTGAAGAGAAGAATAAA GCTCAGAGATTACAGACAGAATTAGATGTCAGTGAGCAAGTCCAGAGGGATTTTGTAAAGCTTTCACAGA CCCTTCAGGTGCAGTTAGAGCGGATCCGGCAAGCTGACTCCTTGGAGAGAATCCGGGCAATTCTGAATGA TACTAAACTGACAGACATTAACCAGCTTCCTGAGACA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG224499 representing NM_001083585
Red=Cloning site Green=Tags(s) MAQPGPASQPDVSLQQRVAELEKINAEFLRAQQQLEQEFNQKRAKFKELYLAKEEDLKRQNAVLQAAQDD LGHLRTQLWEAQAEMENIKAIATVSENTKQEAIDEVKRQWREEVASLQAVMKETVRDYEHQFHLRLEQER TQWAQYRESAEREIADLRRRLSEGQEEENLENEMKKAQEDAEKLRSVVMPMEKEIAALKDKLTEAEDKIK ELEASKVKELNHYLEAEKSCRTDLEMYVAVLNTQKSVLQEDAEKLRKELHEVCHLLEQERQQHNQLKHTW QKANDQFLESQRLLMRDMQRMEIVLTSEQLRQVEELKKKDQEDDEQQRLNKRKDHKKADVEEEIKIPVVC ALTQEESSAQLSNEEEHLDSTRGSVHSLDAGLLLPSGDPFSKSDNDMFKDGLRRAQSTDSLGTSGSLQSK ALGYNYKAKSAGNLDESDFGPLVGADSVSENFDTASLGSLQMPSGFMLTKDQERAIKAMTPEQEETASLL SSVTQGMESAYVSPSGYRLVSETEWNLLQKEVHNAGNKLGRRCDMCSNYEKQLQGIQIQEAETRDQVKKL QLMLRQANDQLEKTMKDKQELEDFIKQSSEDSSHQISALVLRAQASEILLEELQQGLSQAKRDVQEQMAV LMQSREQVSEELVRLQKDNDSLQGKHSLHVSLQQAEDFILPDTTEALRELVLKYREDIINVRTAADHVEE KLKAEILFLKEQIQAEQCLKENLEETLQLEIENCKEEIASISSLKAELERIKVEKGQATVEQLMFEEKNK AQRLQTELDVSEQVQRDFVKLSQTLQVQLERIRQADSLERIRAILNDTKLTDINQLPET TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001083585 |
| ORF Size | 2487 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001083585.3 |
| RefSeq Size | 5322 bp |
| RefSeq ORF | 2490 bp |
| Locus ID | 9135 |
| UniProt ID | Q15276 |
| Protein Pathways | Endocytosis |
| Gene Summary | Rab effector protein acting as linker between gamma-adaptin, RAB4A and RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Involved in KCNH1 channels trafficking to and from the cell membrane (PubMed:22841712). Stimulates RABGEF1 mediated nucleotide exchange on RAB5A. Mediates the traffic of PKD1:PKD2 complex from the endoplasmic reticulum through the Golgi to the cilium (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224499 | RABEP1 (Myc-DDK-tagged)-Human rabaptin, RAB GTPase binding effector protein 1 (RABEP1), transcript variant 2 |
CNY 8,650.00 |
|
| RC224499L3 | Lenti-ORF clone of RABEP1 (Myc-DDK-tagged)-Human rabaptin, RAB GTPase binding effector protein 1 (RABEP1), transcript variant 2 |
CNY 10,550.00 |
|
| RC224499L4 | Lenti-ORF clone of RABEP1 (mGFP-tagged)-Human rabaptin, RAB GTPase binding effector protein 1 (RABEP1), transcript variant 2 |
CNY 10,550.00 |
|
| SC316071 | RABEP1 (untagged)-Human rabaptin, RAB GTPase binding effector protein 1 (RABEP1), transcript variant 2 |
CNY 15,870.00 |
