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alpha 1b Adrenergic Receptor (ADRA1B) (NM_000679) Human Tagged ORF Clone
CAT＃: RG223974
- TrueORF®
ADRA1B (tGFP-tagged) - Human adrenergic, alpha-1B-, receptor (ADRA1B)
ORF Plasmid: DDK
"NM_000679" in other vectors (6)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ADRA1; ALPHA1BAR |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG223974 representing NM_000679
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCCCGACCTGGACACCGGCCACAACACATCAGCACCTGCCCACTGGGGAGAGTTGAAAAATGCCA ACTTCACTGGCCCCAACCAGACCTCGAGCAACTCCACACTGCCCCAGCTGGACATCACCAGGGCCATCTC TGTGGGCCTGGTGCTGGGCGCCTTCATCCTCTTTGCCATCGTGGGCAACATCCTAGTCATCTTGTCTGTG GCCTGCAACCGGCACCTGCGGACGCCCACCAACTACTTCATTGTCAACCTGGCCATGGCCGACCTGCTGT TGAGCTTCACCGTCCTGCCCTTCTCAGCGGCCCTAGAGGTGCTCGGCTACTGGGTGCTGGGGCGGATCTT CTGTGACATCTGGGCAGCCGTGGATGTCCTGTGCTGCACAGCGTCCATTCTGAGCCTGTGCGCCATCTCC ATCGATCGCTACATCGGGGTGCGCTACTCTCTGCAGTATCCCACGCTGGTCACCCGGAGGAAGGCCATCT TGGCGCTGCTCAGTGTCTGGGTCTTGTCCACCGTCATCTCCATCGGGCCTCTCCTTGGGTGGAAGGAGCC GGCACCCAACGATGACAAGGAGTGCGGGGTCACCGAAGAACCCTTCTATGCCCTCTTCTCCTCTCTGGGC TCCTTCTACATCCCTCTGGCGGTCATTCTAGTCATGTACTGCCGTGTCTATATAGTGGCCAAGAGAACCA CCAAGAACCTAGAGGCAGGAGTCATGAAGGAGATGTCCAACTCCAAGGAGCTGACCCTGAGGATCCATTC CAAGAACTTTCACGAGGACACCCTTAGCAGTACCAAGGCCAAGGGCCACAACCCCAGGAGTTCCATAGCT GTCAAACTTTTTAAGTTCTCCAGGGAAAAGAAAGCAGCTAAGACGTTGGGCATTGTGGTCGGTATGTTCA TCTTGTGCTGGCTACCCTTCTTCATCGCTCTACCGCTTGGCTCCTTGTTCTCCACCCTGAAGCCCCCCGA CGCCGTGTTCAAGGTGGTGTTCTGGCTGGGCTACTTCAACAGCTGCCTCAACCCCATCATCTACCCATGC TCCAGCAAGGAGTTCAAGCGCGCTTTCGTGCGCATCCTCGGGTGCCAGTGCCGCGGCCGCGGCCGCCGCC GACGCCGCCGCCGCCGTCGCCTGGGCGGCTGCGCCTACACCTACCGGCCGTGGACGCGCGGCGGCTCGCT GGAGCGCTCGCAGTCGCGCAAGGACTCGCTGGACGACAGCGGCAGCTGCCTGAGCGGCAGCCAGCGGACC TTGCCCTCGGCCTCGCCGAGCCCGGGCTACCTGGGCCGCGGCGCGCCACCGCCAGTCGAGCTGTGCGCCT TCCCCGAGTGGAAGGCGCCCGGCGCCCTCCTGAGCCTGCCCGCGCCTGAGCCCCCCGGCCGCCGCGGCCG CCACGACTCGGGCCCGCTCTTCACCTTCAAGCTCCTGACCGAGCCCGAGAGCCCCGGGACCGACGGCGGC GCCAGCAACGGAGGCTGCGAGGCCGCGGCCGACGTGGCCAACGGGCAGCCGGGCTTCAAAAGCAACATGC CCCTGGCGCCCGGGCAGTTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223974 representing NM_000679
Red=Cloning site Green=Tags(s) MNPDLDTGHNTSAPAHWGELKNANFTGPNQTSSNSTLPQLDITRAISVGLVLGAFILFAIVGNILVILSV ACNRHLRTPTNYFIVNLAMADLLLSFTVLPFSAALEVLGYWVLGRIFCDIWAAVDVLCCTASILSLCAIS IDRYIGVRYSLQYPTLVTRRKAILALLSVWVLSTVISIGPLLGWKEPAPNDDKECGVTEEPFYALFSSLG SFYIPLAVILVMYCRVYIVAKRTTKNLEAGVMKEMSNSKELTLRIHSKNFHEDTLSSTKAKGHNPRSSIA VKLFKFSREKKAAKTLGIVVGMFILCWLPFFIALPLGSLFSTLKPPDAVFKVVFWLGYFNSCLNPIIYPC SSKEFKRAFVRILGCQCRGRGRRRRRRRRRLGGCAYTYRPWTRGGSLERSQSRKDSLDDSGSCLSGSQRT LPSASPSPGYLGRGAPPPVELCAFPEWKAPGALLSLPAPEPPGRRGRHDSGPLFTFKLLTEPESPGTDGG ASNGGCEAAADVANGQPGFKSNMPLAPGQF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000679 |
| ORF Size | 1560 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000679.3, NP_000670.1 |
| RefSeq Size | 2272 bp |
| RefSeq ORF | 1563 bp |
| Locus ID | 147 |
| UniProt ID | P35368 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | Calcium signaling pathway, Neuroactive ligand-receptor interaction, Vascular smooth muscle contraction |
| Gene Summary | Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1B-adrenergic receptor, which induces neoplastic transformation when transfected into NIH 3T3 fibroblasts and other cell lines. Thus, this normal cellular gene is identified as a protooncogene. This gene comprises 2 exons and a single large intron of at least 20 kb that interrupts the coding region. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223974 | ADRA1B (Myc-DDK-tagged)-Human adrenergic, alpha-1B-, receptor (ADRA1B) |
CNY 3,872.00 |
|
| RC223974L1 | Lenti ORF clone of Human adrenergic, alpha-1B-, receptor (ADRA1B), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC223974L2 | Lenti ORF clone of Human adrenergic, alpha-1B-, receptor (ADRA1B), mGFP tagged |
CNY 5,890.00 |
|
| RC223974L3 | Lenti ORF clone of Human adrenergic, alpha-1B-, receptor (ADRA1B), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC223974L4 | Lenti ORF clone of Human adrenergic, alpha-1B-, receptor (ADRA1B), mGFP tagged |
CNY 5,890.00 |
|
| SC110821 | ADRA1B (untagged)-Human adrenergic, alpha-1B-, receptor (ADRA1B) |
CNY 3,880.00 |
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