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PP4R1 (PPP4R1) (NM_001042388) Human Tagged ORF Clone
CAT＃: RG223947
- TrueORF®
PPP4R1 (tGFP-tagged) - Human protein phosphatase 4, regulatory subunit 1 (PPP4R1), transcript variant 1
ORF Plasmid: DDK
"NM_001042388" in other vectors (4)
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CNY 10,450.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MEG1; PP4(Rmeg); PP4R1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG223947 representing NM_001042388
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACCTCTCGCTGCTTCAGGAGGACCTGCAGGAGGACGCAGACGGATTTGGTGTGGATGACTACA GCTCAGAGTCTGATGTGATTATTATACCTTCAGCCCTGGACTTTGTCTCACAAGATGAAATGTTGACGCC CCTGGGGAGATTGGACAAGTATGCTGCAAGTGAGAACATATTTAACAGACAAATGGTGGCCCGGAGTTTG CTCGATACCTTGAGGGAAGTCTGCGATGATGAAAGAGATTGTATTGCTGTTTTGGAAAGAATTAGCAGAT TGGCCGATGATTCAGAACCAACTGTGAGAGCGGAGCTGATGGAACAGGTGCCTCACATCGCACTGTTTTG TCAAGAAAACCGGCCTTCAATACCATATGCTTTTTCAAAATTCTTACTACCTATTGTGGTTAGATACCTT GCAGATCAGAATAATCAGGTGAGGAAAACAAGTCAGGCAGCTTTGCTGGCTCTGTTGGAGCAGGAGCTCA TTGAACGATTTGATGTGGAGACCAAAGTGTGCCCTGTCCTCATAGAGCTGACAGCCCCAGATAGCAATGA TGATGTGAAAACAGAAGCTGTGGCTATAATGTGCAAAATGGCTCCCATGGTTGGGAAGGATATTACAGAG CGTCTTATCCTCCCTAGGTTTTGTGAGATGTGCTGCGATTGCAGAATGTTTCACGTTCGAAAGGTCTGTG CTGCCAATTTTGGAGATATTTGCAGTGTAGTTGGCCAGCAAGCTACTGAAGAAATGTTGCTGCCCAGATT TTTCCAGCTTTGTTCTGATAATGTATGGGGAGTCCGAAAGGCTTGTGCTGAATGCTTCATGGCGGTTTCA TGTGCAACATGTCAAGAAATCCGACGGACCAAATTATCAGCACTTTTTATTAATTTGATCAGTGATCCTT CACGTTGGGTTCGCCAAGCAGCTTTTCAGTCTCTGGGACCTTTCATATCTACTTTTGCTAATCCATCTAG CTCAGGCCAGTATTTTAAAGAAGAAAGCAAAAGTTCAGAAGAGATGTCAGTAGAAAACAAAAATAGGACC AGAGATCAAGAAGCCCCAGAGGATGTACAAGTCAGGCCAGAGGATACTCCTTCAGATCTCAGTGTTAGTA ATTCCAGTGTCATACTGGAAAACACGATGGAAGACCATGCTGCTGAGGCATCCGGGAAGCCTCTAGGTGA AATTAGTGTTCCACTGGACAGCTCTTTACTTTGTACTTTGTCCTCAGAATCTCACCAGGAAGCAGCTAGT AATGAGAATGATAAAAAACCTGGTAACTACAAATCTATGTTACGACCAGAGGTTGGCACCACTTCACAAG ATTCAGCTCTCTTAGATCAGGAATTGTATAACTCCTTCCATTTCTGGAGGACTCCTCTTCCTGAAATAGA TCTAGACATAGAGCTTGAACAGAACTCTGGGGGAAAACCCAGCCCAGAGGGACCAGAGGAAGAATCTGAG GGCCCTGTGCCCAGTTCTCCAAACATCACCATGGCCACCAGAAAGGAACTGGAAGAAATGATAGAAAATC TAGAGCCCCACATTGATGATCCAGATGTTAAAGCACAAGTGGAAGTGCTGTCCGCTGCACTACGTGCTTC CAGCCTGGATGCACATGAAGAGACCATCAGTATAGAAAAGAGAAGTGATTTGCAAGATGAACTGGATATA AATGAGCTACCAAATTGTAAAATAAATCAAGAAGATTCTGTGCCTTTAATCAGCGATGCTGTTGAGAATA TGGACTCCACTCTTCACTATATTCACAGCGATTCAGACTTGAGCAACAATAGCAGTTTTAGCCCTGATGA GGAAAGGAGAACTAAAGTACAAGATGTTGTACCTCAGGCGTTGTTAGATCAGTATTTATCTATGACTGAC CCTTCTCGTGCACAGACGGTTGACACTGAAATTGCTAAGCACTGTGCATATAGCCTCCCTGGTGTGGCCT TGACACTCGGAAGACAGAATTGGCACTGCCTGAGAGAGACGTATGAGACTCTGGCCTCAGACATGCAGTG GAAAGTTCGACGAACTCTAGCATTCTCCATCCACGAGCTTGCAGTTATTCTTGGAGATCAATTGACAGCT GCAGATCTGGTTCCAATTTTTAATGGATTTTTAAAAGACCTCGATGAAGTCAGGATAGGTGTTCTTAAAC ACTTGCATGATTTTCTGAAGCTTCTTCATATTGACAAAAGAAGAGAATATCTTTATCAACTTCAGGAGTT TTTGGTGACAGATAATAGTAGAAATTGGCGGTTTCGAGCTGAACTGGCTGAACAGCTGATTTTACTTCTA GAGTTATATAGTCCCAGAGATGTTTATGACTATTTACGTCCCATTGCTCTGAATCTGTGTGCAGACAAAG TTTCTTCTGTTCGTTGGATTTCCTACAAGTTGGTCAGCGAGATGGTGAAGAAGCTGCACGCGGCAACACC ACCAACGTTCGGAGTGGACCTCATCAATGAGCTTGTGGAGAACTTTGGCAGATGTCCCAAGTGGTCTGGT CGGCAAGCCTTTGTCTTTGTCTGCCAGACTGTCATTGAGGATGACTGCCTTCCCATGGACCAGTTTGCTG TGCATCTCATGCCGCATCTGCTAACCTTAGCAAATGACAGGGTTCCTAACGTGCGAGTGCTGCTTGCAAA GACATTAAGACAAACTCTACTAGAAAAAGACTATTTCTTGGCCTCTGCCAGCTGCCACCAGGAGGCTGTG GAGCAGACCATCATGGCTCTTCAGATGGACCGTGACAGCGATGTCAAGTATTTTGCAAGCATCCACCCTG CCAGTACCAAAATCTCCGAAGATGCCATGAGCACAGCGTCCTCAACCTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223947 representing NM_001042388
Red=Cloning site Green=Tags(s) MADLSLLQEDLQEDADGFGVDDYSSESDVIIIPSALDFVSQDEMLTPLGRLDKYAASENIFNRQMVARSL LDTLREVCDDERDCIAVLERISRLADDSEPTVRAELMEQVPHIALFCQENRPSIPYAFSKFLLPIVVRYL ADQNNQVRKTSQAALLALLEQELIERFDVETKVCPVLIELTAPDSNDDVKTEAVAIMCKMAPMVGKDITE RLILPRFCEMCCDCRMFHVRKVCAANFGDICSVVGQQATEEMLLPRFFQLCSDNVWGVRKACAECFMAVS CATCQEIRRTKLSALFINLISDPSRWVRQAAFQSLGPFISTFANPSSSGQYFKEESKSSEEMSVENKNRT RDQEAPEDVQVRPEDTPSDLSVSNSSVILENTMEDHAAEASGKPLGEISVPLDSSLLCTLSSESHQEAAS NENDKKPGNYKSMLRPEVGTTSQDSALLDQELYNSFHFWRTPLPEIDLDIELEQNSGGKPSPEGPEEESE GPVPSSPNITMATRKELEEMIENLEPHIDDPDVKAQVEVLSAALRASSLDAHEETISIEKRSDLQDELDI NELPNCKINQEDSVPLISDAVENMDSTLHYIHSDSDLSNNSSFSPDEERRTKVQDVVPQALLDQYLSMTD PSRAQTVDTEIAKHCAYSLPGVALTLGRQNWHCLRETYETLASDMQWKVRRTLAFSIHELAVILGDQLTA ADLVPIFNGFLKDLDEVRIGVLKHLHDFLKLLHIDKRREYLYQLQEFLVTDNSRNWRFRAELAEQLILLL ELYSPRDVYDYLRPIALNLCADKVSSVRWISYKLVSEMVKKLHAATPPTFGVDLINELVENFGRCPKWSG RQAFVFVCQTVIEDDCLPMDQFAVHLMPHLLTLANDRVPNVRVLLAKTLRQTLLEKDYFLASASCHQEAV EQTIMALQMDRDSDVKYFASIHPASTKISEDAMSTASSTY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001042388 |
| ORF Size | 2850 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001042388.3 |
| RefSeq Size | 3980 bp |
| RefSeq ORF | 2853 bp |
| Locus ID | 9989 |
| UniProt ID | Q8TF05 |
| Protein Families | Druggable Genome, Phosphatase |
| Gene Summary | This gene encodes one of several alternate regulatory subunits of serine/threonine protein phosphatase 4 (PP4). The protein features multiple HEAT repeats. This protein forms a complex with PP4RC. This complex may have a distinct role from other PP4 complexes, including regulation of HDAC3 (Zhang et al., PMID: 15805470). There is also a transcribed pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223947 | PPP4R1 (Myc-DDK-tagged)-Human protein phosphatase 4, regulatory subunit 1 (PPP4R1), transcript variant 1 |
CNY 6,960.00 |
|
| RC223947L3 | Lenti-ORF clone of PPP4R1 (Myc-DDK-tagged)-Human protein phosphatase 4, regulatory subunit 1 (PPP4R1), transcript variant 1 |
CNY 11,400.00 |
|
| RC223947L4 | Lenti-ORF clone of PPP4R1 (mGFP-tagged)-Human protein phosphatase 4, regulatory subunit 1 (PPP4R1), transcript variant 1 |
CNY 11,400.00 |
|
| SC311319 | PPP4R1 (untagged)-Human protein phosphatase 4, regulatory subunit 1 (PPP4R1), transcript variant 1 |
CNY 18,340.00 |
