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ACAT1 (ACACA) (NM_198837) Human Tagged ORF Clone
CAT＃: RG223932
- TrueORF®
ACACA (tGFP-tagged) - Human acetyl-CoA carboxylase alpha (ACACA), transcript variant 4
ORF Plasmid: DDK
"NM_198837" in other vectors (2)
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CNY 20,900.00
货期*
11周
规格
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经常一起买 (5)
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ACAC; ACACAD; ACC; ACC1; ACCA |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG223932 representing NM_198837
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGTTCTCCAGAGGAAAATAAGGAAATGAGATATTACATGCTTCAAAGGTCCAGCATGTCTGGCT TGCACCTAGTAAAGCAGGGCCGAGACAGAAAGAAAATAGATTCTCAACGAGATTTCACTGTGGCTTCTCC AGCAGAATTTGTTACTCGCTTTGGGGGAAATAAAGTGATTGAGAAGGTTCTTATTGCTAACAATGGCATT GCAGCAGTGAAATGCATGCGGTCTATCCGTAGGTGGTCTTATGAAATGTTTCGAAATGAACGTGCAATTA GATTCGTTGTCATGGTCACACCTGAAGACCTTAAAGCCAATGCAGAATACATTAAGATGGCAGATCACTA TGTGCCAGTGCCTGGAGGACCAAACAACAACAACTATGCAAATGTGGAATTAATTCTTGATATTGCTAAA AGGATCCCAGTACAAGCAGTGTGGGCTGGCTGGGGTCATGCTTCTGAGAATCCCAAACTACCGGAACTTC TCTTGAAAAATGGCATTGCCTTCATGGGTCCTCCAAGCCAGGCCATGTGGGCTTTAGGGGATAAGATTGC ATCTTCCATAGTGGCTCAAACTGCAGGTATCCCAACTCTTCCCTGGAGCGGCAGTGGTCTTCGTGTGGAC TGGCAGGAAAATGATTTTTCAAAACGTATCTTAAATGTTCCCCAGGAGCTATATGAAAAAGGTTATGTGA AAGATGTGGATGATGGGCTACAGGCAGCTGAGGAAGTTGGATATCCAGTAATGATCAAGGCCTCAGAGGG AGGAGGAGGGAAGGGAATTAGAAAAGTCAACAATGCAGATGACTTCCCTAATCTCTTCAGACAGGTTCAA GCTGAAGTTCCTGGATCTCCCATATTTGTGATGAGACTAGCCAAACAATCTCGTCATCTGGAGGTGCAGA TCTTAGCGGACCAATATGGCAATGCTATCTCTTTGTTTGGTCGTGATTGCTCTGTACAACGCAGGCATCA GAAGATTATTGAAGAAGCACCTGCTACTATTGCTACTCCAGCAGTATTTGAACACATGGAACAGTGTGCG GTGAAACTTGCCAAAATGGTGGGTTATGTGAGTGCTGGGACTGTGGAATACCTGTACAGCCAGGATGGCA GCTTCTACTTTCTGGAATTGAATCCTCGGCTGCAGGTAGAGCACCCTTGTACAGAGATGGTGGCTGATGT CAATCTCCCTGCAGCACAGCTCCAGATTGCCATGGGGATTCCTCTATATAGAATCAAGGATATCCGTATG ATGTATGGGGTATCTCCCTGGGGTGATTCTCCCATTGATTTTGAAGATTCTGCACACGTTCCTTGTCCAA GGGGCCATGTTATTGCTGCTCGGATCACTAGTGAAAATCCAGATGAGGGTTTTAAGCCCAGCTCAGGAAC AGTTCAGGAGCTAAATTTCCGCAGCAATAAGAATGTTTGGGGATATTTCAGTGTTGCTGCTGCAGGGGGA CTTCATGAATTTGCTGATTCTCAGTTTGGTCACTGCTTTTCTTGGGGAGAAAACAGAGAAGAGGCAATTT CAAACATGGTGGTGGCTTTGAAGGAGCTGTCTATTCGGGGTGACTTTCGAACTACAGTTGAATACCTGAT CAAATTGTTAGAGACTGAAAGCTTTCAGATGAACAGAATTGATACTGGCTGGCTGGACAGACTGATAGCA GAAAAAGTACAGGCTGAGCGACCTGACACCATGTTGGGGGTTGTGTGTGGTGCCCTCCACGTGGCAGATG TGAGCCTGCGGAATAGCGTCTCTAACTTCCTTCACTCCTTAGAAAGGGGTCAAGTCCTTCCTGCTCATAC ACTTCTGAATACAGTAGATGTTGAACTTATCTATGAGGGAGTCAAGTATGTACTTAAGGTGACTCGACAG TCCCCCAACTCCTATGTGGTGATCATGAATGGCTCATGTGTAGAAGTAGATGTACATCGGCTGAGTGACG GTGGACTGCTCTTGTCCTATGATGGCAGCAGTTATACTACGTATATGAAAGAGGAAGTGGATAGATATCG CATCACAATTGGCAATAAAACCTGTGTGTTTGAGAAGGAAAATGACCCATCGGTGATGCGCTCACCTTCT GCTGGGAAGTTAATCCAGTACATTGTAGAAGATGGAGGTCATGTGTTTGCCGGCCAGTGCTATGCTGAGA TTGAGGTAATGAAGATGGTAATGACCTTAACAGCTGTGGAGTCTGGCTGTATCCATTACGTCAAGCGACC TGGAGCAGCTCTTGACCCTGGCTGTGTACTAGCCAAAATGCAACTGGACAACCCCAGCAAGGTTCAGCAG GCTGAACTTCACACAGGTAGTCTGCCACGGATCCAGAGCACGGCACTCAGAGGCGAGAAACTCCATCGAG TGTTCCATTATGTCCTGGATAATCTGGTCAATGTAATGAATGGATACTGCCTTCCAGATCCTTTCTTTAG CAGCAAGGTAAAAGACTGGGTAGAGCGATTGATGAAAACCCTCAGAGATCCCTCCCTGCCTCTCCTAGAA TTGCAAGATATTATGACCAGTGTGTCTGGCCGCATTCCCCCCAATGTGGAGAAGTCTATCAAGAAGGAAA TGGCTCAGTATGCTAGCAACATCACATCAGTCCTCTGTCAGTTTCCCAGCCAGCAGATTGCAAACATCCT AGATAGCCATGCAGCTACATTGAACCGGAAATCTGAACGGGAAGTCTTCTTTATGAATACTCAGAGCATT GTTCAGCTGGTACAGAGGTACCGAAGTGGCATCCGAGGCCACATGAAGGCTGTGGTGATGGATCTGCTCC GGCAGTACCTGCGAGTAGAGACACAATTCCAGAATGGTCACTATGACAAATGTGTATTCGCCCTCCGAGA AGAGAATAAAAGTGACATGAACACTGTACTGAACTACATCTTCTCTCACGCTCAAGTCACCAAGAAGAAT CTTCTGGTCACAATGCTTATTGATCAGTTGTGTGGCCGGGACCCTACTCTCACTGATGAGCTGCTGAATA TTCTCACAGAGCTAACTCAACTCAGTAAGACCACCAATGCCAAAGTAGCACTTCGAGCACGCCAGGTTCT TATTGCCTCCCATTTGCCATCATATGAGCTTCGCCATAACCAAGTAGAGTCTATCTTCCTATCAGCTATT GACATGTATGGACATCAATTTTGCATTGAGAACCTGCAGAAACTCATCCTATCAGAAACATCTATTTTTG ATGTCCTACCAAACTTCTTCTATCACAGCAACCAAGTAGTGAGGATGGCAGCTCTGGAGGTGTATGTTCG AAGGGCTTATATTGCCTATGAACTTAACAGCGTACAACACCGCCAGCTTAAGGACAACACCTGTGTGGTG GAATTCCAGTTCATGCTGCCCACATCTCATCCAAACAGAGGGAACATCCCTACGCTAAACAGAATGTCCT TCTCCTCCAACCTCAACCACTATGGCATGACCCATGTAGCTAGTGTCAGCGATGTACTGTTGGACAACTC ATTCACTCCACCTTGTCAGCGGATGGGCGGAATGGTCTCTTTTCGGACTTTTGAAGATTTTGTCAGGATC TTTGATGAAGTGATGGGCTGCTTCTCTGACTCCCCACCCCAGAGTCCCACATTCCCTGAGGCAGGTCACA CGTCTCTTTATGATGAGGATAAGGTTCCCAGGGATGAACCAATTCACATTCTCAATGTGGCTATCAAGAC TGACTGTGATATTGAGGATGACAGGCTGGCAGCTATGTTCAGAGAATTTACCCAGCAAAATAAAGCTACC CTGGTTGACCATGGGATCCGGCGCCTTACTTTCCTGGTTGCACAAAAGGATTTCAGAAAGCAGGTCAACT ATGAGGTGGATCGGAGATTTCATAGAGAATTCCCTAAATTTTTTACATTCCGAGCAAGGGATAAGTTTGA GGAGGATCGTATCTATCGTCATCTGGAGCCTGCTCTGGCTTTCCAGTTAGAGCTGAACCGGATGAGAAAT TTTGACCTCACTGCCATTCCATGTGCTAATCACAAGATGCACCTGTATCTCGGGGCAGCCAAGGTGGAAG TGGGCACAGAAGTGACAGACTACAGGTTCTTTGTTCGTGCAATCATCAGGCATTCTGATCTGGTCACCAA GGAAGCTTCTTTTGAATATCTGCAAAATGAAGGGGAGCGGCTACTCCTGGAAGCCATGGATGAGTTGGAA GTTGCTTTTAACAATACAAATGTCCGCACTGACTGTAACCACATCTTCCTCAACTTTGTGCCCACGGTTA TCATGGACCCATCAAAGATTGAGGAATCCGTGCGGAGCATGGTAATGCGGTATGGAAGTCGCCTGTGGAA ATTGCGCGTCCTCCAGGCAGAACTGAAAATCAACATTCGCCTGACGCCAACTGGAAAAGCAATTCCCATC CGCCTCTTCCTGACAAACGAGTCTGGCTATTACTTGGATATCAGCCTATACAAGGAAGTGACTGACTCCA GGACAGCACAGATCATGTTTCAGGCATATGGAGACAAACAGGGACCACTGCATGGAATGTTAATCAATAC TCCATATGTGACCAAAGACCTGCTGCAATCAAAGAGGTTCCAGGCACAATCCTTAGGGACAACATACATA TATGATATCCCAGAGATGTTTCGGCAGTCCCTGATCAAACTCTGGGAGTCTATGTCCACTCAAGCATTTC TTCCATCTCCCCCTCTGCCTTCTGACATGCTGACTTACACTGAACTGGTACTGGATGATCAAGGTCAGCT GGTCCACATGAACAGGCTTCCAGGAGGAAATGAGATTGGCATGGTAGCTTGGAAAATGACCTTTAAAAGT CCTGAATATCCAGAAGGCCGAGATATCATTGTTATTGGCAATGACATCACATACCGAATTGGGTCCTTTG GGCCTCAAGAGGATTTGTTATTTCTCAGAGCTTCCGAACTTGCTAGGGCAGAAGGTATTCCACGCATCTA TGTATCAGCCAACAGTGGAGCAAGAATCGGACTGGCAGAAGAAATTCGCCATATGTTTCATGTGGCCTGG GTAGATCCTGAGGATCCTTACAAGGGATACAGGTATTTATATCTGACTCCTCAAGATTATAAGAGAGTCA GTGCTCTCAACTCTGTCCATTGTGAACACGTGGAAGATGAAGGAGAATCCAGGTACAAGATAACAGATAT TATTGGGAAAGAAGAGGGAATTGGACCCGAGAACCTTCGAGGTTCTGGAATGATTGCTGGAGAATCCTCA TTGGCCTATAATGAGATCATTACCATCAGCCTGGTGACGTGCCGGGCCATTGGGATTGGGGCTTACCTTG TCCGGCTGGGACAGAGAACCATCCAGGTTGAGAATTCTCACTTAATTCTAACAGGAGCTGGAGCCCTCAA CAAAGTCCTCGGGCGGGAAGTGTACACCTCCAATAACCAGCTGGGGGGCATCCAGATTATGCACAACAAT GGGGTGACCCACTGCACTGTGTGTGATGACTTTGAAGGGGTTTTCACTGTCCTGCACTGGCTGTCTTACA TGCCCAAGAGCGTGCACAGTTCAGTTCCTCTTCTGAACTCAAAGGATCCTATAGACAGAATCATCGAGTT TGTTCCCACAAAGACCCCATACGATCCTCGATGGATGCTAGCAGGCCGTCCTCACCCAACCCAAAAAGGT CAGTGGTTGAGTGGCTTTTTTGACTATGGATCTTTCTCAGAGATTATGCAGCCCTGGGCACAGACTGTGG TGGTTGGTAGAGCCAGGCTAGGAGGAATACCTGTGGGAGTTGTTGCTGTAGAAACCCGAACAGTAGAACT AAGTATCCCAGCTGATCCAGCAAACCTGGATTCTGAAGCCAAGATAATCCAGCAGGCTGGCCAGGTTTGG TTCCCAGATTCTGCGTTTAAGACGTATCAGGCCATCAAGGACTTCAACCGGGAAGGGCTGCCTCTGATGG TCTTTGCCAACTGGAGAGGCTTCTCTGGTGGAATGAAAGATATGTACGACCAAGTGCTGAAGTTTGGTGC TTACATTGTGGATGGCTTGAGGGAGTGCTGCCAGCCTGTGCTGGTTTACATTCCTCCCCAGGCTGAGCTG CGGGGTGGCTCCTGGGTGGTGATTGACTCCTCCATCAACCCCCGGCACATGGAGATGTATGCTGACCGAG AAAGCAGGGGATCTGTTCTGGAGCCAGAAGGGACAGTAGAAATCAAATTCCGCAGAAAGGATCTGGTGAA AACCATGCGTCGGGTGGACCCAGTCTACATCCACTTGGCTGAGCGATTGGGGACCCCAGAGCTAAGCACA GCTGAGCGGAAGGAGTTGGAGAACAAGTTGAAGGAGCGGGAGGAATTCCTAATTCCCATTTACCATCAGG TAGCCGTGCAGTTTGCTGACTTGCACGACACACCAGGCCGGATGCAGGAGAAGGGTGTTATTAGCGATAT CCTGGATTGGAAAACATCCCGTACCTTCTTCTACTGGCGGCTGAGGCGTCTTCTGCTGGAGGACCTGGTC AAGAAGAAAATCCACAATGCCAACCCTGAGCTGACTGATGGCCAGATTCAAGCCATGTTAAGGCGCTGGT TTGTGGAAGTGGAAGGAACAGTGAAGGCTTATGTTTGGGACAATAATAAGGATCTGGCGGAGTGGCTAGA GAAACAGCTGACAGAGGAGGATGGTGTTCACTCGGTAATAGAGGAAAACATCAAATGCATCAGCAGAGAC TACGTCCTCAAGCAAATCCGCAGCTTGGTCCAGGCCAATCCAGAGGTTGCCATGGATTCCATCATCCATA TGACGCAGCACATATCACCCACTCAGCGAGCAGAAGTCATACGGATCCTCTCCACAATGGATTCCCCTTC CACG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223932 representing NM_198837
Red=Cloning site Green=Tags(s) MEGSPEENKEMRYYMLQRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGI AAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAK RIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVD WQENDFSKRILNVPQELYEKGYVKDVDDGLQAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQ AEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATPAVFEHMEQCA VKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRM MYGVSPWGDSPIDFEDSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGG LHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQMNRIDTGWLDRLIA EKVQAERPDTMLGVVCGALHVADVSLRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGVKYVLKVTRQ SPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVMRSPS AGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAVESGCIHYVKRPGAALDPGCVLAKMQLDNPSKVQQ AELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSKVKDWVERLMKTLRDPSLPLLE LQDIMTSVSGRIPPNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSI VQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKN LLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAI DMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVV EFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMTHVASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRI FDEVMGCFSDSPPQSPTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQQNKAT LVDHGIRRLTFLVAQKDFRKQVNYEVDRRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRN FDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELE VAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPI RLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYI YDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMTFKS PEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIYVSANSGARIGLAEEIRHMFHVAW VDPEDPYKGYRYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGIGPENLRGSGMIAGESS LAYNEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNN GVTHCTVCDDFEGVFTVLHWLSYMPKSVHSSVPLLNSKDPIDRIIEFVPTKTPYDPRWMLAGRPHPTQKG QWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVW FPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDGLRECCQPVLVYIPPQAEL RGGSWVVIDSSINPRHMEMYADRESRGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIHLAERLGTPELST AERKELENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVISDILDWKTSRTFFYWRLRRLLLEDLV KKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLAEWLEKQLTEEDGVHSVIEENIKCISRD YVLKQIRSLVQANPEVAMDSIIHMTQHISPTQRAEVIRILSTMDSPST TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_198837 |
| ORF Size | 6864 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_198837.1, NP_942134.1 |
| RefSeq Size | 9655 bp |
| RefSeq ORF | 6867 bp |
| Locus ID | 31 |
| UniProt ID | Q13085 |
| Protein Families | Druggable Genome |
| Protein Pathways | Fatty acid biosynthesis, Insulin signaling pathway, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism |
| Gene Summary | Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
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