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PAICS (NM_001079525) Human Tagged ORF Clone
CAT＃: RG223925
- TrueORF®
PAICS (tGFP-tagged) - Human phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase (PAICS), transcript variant 1
ORF Plasmid: DDK
"NM_001079525" in other vectors (6)
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CNY 7,088.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ADE2; ADE2H1; AIRC; PAIS |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG223925 representing NM_001079525
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACAGCTGAGGTACTGAACATTGGTAAAAAATTATATGAGGGTAAAACAAAAGAAGTCTACGAAT TGTTAGACAGTCCAGGAAAAGTCCTCCTGCAGTCCAAGGACCAGATTACAGCAGGAAATGCAGCTAGAAA AAACCACCTGGAAGGAAAAGCTGCAATCTCAAATAAAATCACCAGTTGTATTTTTCAGTTATTACAGGAA GCAGTTACCTCATATAAGTCAAATCGTATTAAAACTGCCTTCACCAGAAAATGTGGGGAGACAGCTTTCA TTGCACCGCAGTGTGAAATGATTCCAATTGAATGGGTTTGCAGAAGAATAGCAACTGGTTCTTTTCTCAA AAGAAATCCTGGTGTCAAGGAAGGATATAAGTTTTACCCACCTAAAGTGGAGTTGTTTTTCAAGGATGAT GCCAATAATGACCCACAGTGGTCTGAGGAACAGCTGATTGCTGCAAAATTTTGCTTTGCTGGACTTCTTA TAGGCCAGACTGAAGTGGATATCATGAGTCATGCTACACAGGCTATATTTGAAATACTGGAGAAATCCTG GTTGCCCCAGAATTGTACACTGGTTGATATGAAGATTGAATTTGGTGTTGATGTAACCACCAAAGAAATT GTTCTTGCTGATGTTATTGACAATGATTCCTGGAGACTCTGGCCATCAGGAGATCGAAGCCAACAGAAAG ACAAACAGTCTTATCGGGACCTCAAAGAAGTAACTCCTGAAGGGCTCCAAATGGTAAAGAAAAACTTTGA GTGGGTTGCAGAGAGAGTAGAGTTGCTTTTGAAATCAGAAAGTCAGTGCAGGGTTGTAGTGTTGATGGGC TCTACTTCTGATCTTGGTCACTGTGAAAAAATCAAGAAGGCCTGTGGAAATTTTGGCATTCCATGTGAAC TTCGAGTAACATCTGCGCATAAAGGACCAGATGAAACTCTGAGGATTAAAGCTGAGTATGAAGGGGATGG CATTCCTACTGTATTTGTGGCAGTGGCAGGCAGAAGTAATGGTTTGGGACCAGTGATGTCTGGGAACACT GCATATCCAGTTATCAGCTGTCCTCCCCTCACACCAGACTGGGGAGTTCAGGATGTGTGGTCTTCTCTTC GACTACCCAGTGGTCTTGGCTGTTCAACCGTACTTTCTCCAGAAGGATCAGCTCAATTTGCTGCTCAGAT ATTTGGGTTAAGCAACCATTTGGTATGGAGCAAACTGCGAGCAAGCATTTTGAACACATGGATTTCCTTG AAGCAGGCTGACAAGAAAATCAGAGAATGTAATTTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223925 representing NM_001079525
Red=Cloning site Green=Tags(s) MATAEVLNIGKKLYEGKTKEVYELLDSPGKVLLQSKDQITAGNAARKNHLEGKAAISNKITSCIFQLLQE AVTSYKSNRIKTAFTRKCGETAFIAPQCEMIPIEWVCRRIATGSFLKRNPGVKEGYKFYPPKVELFFKDD ANNDPQWSEEQLIAAKFCFAGLLIGQTEVDIMSHATQAIFEILEKSWLPQNCTLVDMKIEFGVDVTTKEI VLADVIDNDSWRLWPSGDRSQQKDKQSYRDLKEVTPEGLQMVKKNFEWVAERVELLLKSESQCRVVVLMG STSDLGHCEKIKKACGNFGIPCELRVTSAHKGPDETLRIKAEYEGDGIPTVFVAVAGRSNGLGPVMSGNT AYPVISCPPLTPDWGVQDVWSSLRLPSGLGCSTVLSPEGSAQFAAQIFGLSNHLVWSKLRASILNTWISL KQADKKIRECNL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001079525 |
| ORF Size | 1296 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001079525.2 |
| RefSeq Size | 3350 bp |
| RefSeq ORF | 1299 bp |
| Locus ID | 10606 |
| UniProt ID | P22234 |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Purine metabolism |
| Gene Summary | This gene encodes a bifunctional enzyme containing phosphoribosylaminoimidazole carboxylase activity in its N-terminal region and phosphoribosylaminoimidazole succinocarboxamide synthetase in its C-terminal region. It catalyzes steps 6 and 7 of purine biosynthesis. The gene is closely linked and divergently transcribed with a locus that encodes an enzyme in the same pathway, and transcription of the two genes is coordinately regulated. The human genome contains several pseudogenes of this gene. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223925 | PAICS (Myc-DDK-tagged)-Human phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase (PAICS), transcript variant 1 |
CNY 5,488.00 |
|
| RC223925L1 | Lenti ORF clone of Human phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase (PAICS), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC223925L2 | Lenti ORF clone of Human phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase (PAICS), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC223925L3 | Lenti ORF clone of Human phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase (PAICS), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC223925L4 | Lenti ORF clone of Human phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase (PAICS), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| SC315553 | PAICS (untagged)-Human phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase (PAICS), transcript variant 1 |
CNY 6,940.00 |
