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ATP6V0A1 (NM_005177) Human Tagged ORF Clone
CAT＃: RG223769
- TrueORF®
ATP6V0A1 (tGFP-tagged) - Human ATPase, H+ transporting, lysosomal V0 subunit a1 (ATP6V0A1), transcript variant 3
ORF Plasmid: DDK
"NM_005177" in other vectors (4)
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CNY 7,688.00
CNY 9,600.00
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | a1; ATP6N1; ATP6N1A; Stv1; Vph1; VPP1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG223769 representing NM_005177
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGAGCTTTTCCGGAGTGAAGAAATGACACTGGCCCAGCTTTTTCTACAGTCAGAGGCTGCTTATT GTTGTGTCAGTGAATTAGGAGAACTTGGAAAGGTTCAGTTTCGTGACTTAAATCCAGATGTGAATGTTTT CCAACGGAAATTTGTGAATGAAGTTAGAAGATGTGAAGAAATGGATCGAAAGCTTCGATTTGTTGAGAAA GAGATAAGAAAAGCTAACATTCCGATTATGGACACCGGTGAAAACCCAGAGGTTCCCTTCCCCCGGGACA TGATTGACTTAGAGGCCAATTTTGAGAAGATTGAAAATGAACTGAAGGAAATCAACACAAACCAGGAAGC TCTGAAGAGAAACTTCCTGGAACTGACCGAATTAAAATTTATACTTCGCAAAACTCAGCAATTTTTTGAT GAGATGGCGGATCCAGACTTGTTGGAAGAGTCCTCATCCCTCTTGGAGCCAAGTGAGATGGGAAGAGGCA CTCCTTTAAGACTTGGCTTCGTGGCTGGTGTCATTAACCGGGAGCGCATCCCTACTTTTGAGCGCATGCT TTGGCGGGTATGCCGGGGAAATGTGTTCCTGCGACAGGCTGAAATCGAGAACCCCCTGGAGGATCCTGTG ACTGGCGACTACGTGCACAAGTCTGTGTTTATCATTTTCTTCCAAGGCGATCAGCTGAAAAACAGAGTCA AGAAAATCTGTGAAGGGTTCCGAGCCTCACTCTATCCCTGTCCTGAGACACCACAGGAGAGGAAGGAAAT GGCTTCTGGAGTGAATACCAGGATTGATGATCTCCAAATGGTTCTGAATCAAACGGAGGATCACCGCCAG AGGGTTCTGCAGGCAGCTGCTAAGAACATCCGTGTCTGGTTCATCAAAGTGCGGAAGATGAAGGCCATCT ATCACACCCTGAACCTGTGCAACATAGATGTGACTCAGAAATGCTTGATTGCAGAGGTCTGGTGCCCTGT CACCGACCTTGACTCCATCCAGTTTGCACTCAGAAGGGGCACGGAACACAGTGGTTCCACTGTACCTTCC ATTTTGAACAGGATGCAGACAAACCAGACTCCCCCAACCTATAACAAAACCAACAAGTTTACCTATGGCT TTCAGAACATAGTAGATGCTTATGGAATTGGAACTTACCGAGAGATAAATCCAGCTCCGTATACTATTAT CACGTTCCCTTTTCTATTTGCTGTGATGTTTGGAGACTTCGGTCATGGCATTTTAATGACCCTTTTTGCT GTGTGGATGGTACTGAGGGAGAGCCGGATCCTTTCCCAGAAGAATGAGAATGAGATGTTTAGCACTGTGT TCAGTGGTCGATACATTATTTTATTGATGGGTGTGTTCTCCATGTACACTGGCCTCATCTACAATGATTG CTTTTCCAAGTCTCTTAATATCTTTGGGTCATCCTGGAGTGTACGGCCGATGTTTACTTATAATTGGACT GAAGAGACGCTTCGGGGGAACCCTGTTCTACAGCTGAACCCAGCCCTCCCTGGAGTGTTTGGTGGACCAT ACCCTTTTGGCATTGATCCAATTTGGAACATTGCTACCAATAAACTGACGTTCTTGAACTCCTTTAAGAT GAAGATGTCTGTTATCCTTGGTATCATCCATATGCTGTTTGGAGTCAGCCTGAGTCTGTTCAACCATATC TATTTCAAGAAGCCCCTGAATATCTACTTTGGATTTATTCCTGAAATAATCTTCATGACCTCTTTGTTTG GCTATTTGGTTATCCTTATTTTTTACAAGTGGACGGCCTATGATGCTCATACCTCTGAGAATGCACCAAG CCTTCTGATCCATTTCATAAACATGTTCCTCTTTTCCTACCCAGAGTCTGGTTATTCAATGTTGTATTCT GGACAGAAAGGAATTCAGTGTTTCCTGGTAGTGGTTGCACTACTGTGTGTACCTTGGATGCTGCTGTTTA AACCATTGGTCCTTCGCCGTCAGTATTTGAGGAGAAAGCATTTGGGAACTCTCAACTTTGGTGGGATCAG GGTGGGCAACGGACCGACAGAGGAGGATGCTGAGATTATTCAGCATGACCAGCTCTCCACCCACTCAGAG GACGCAGACGAGTTTGACTTTGGGGACACCATGGTCCACCAGGCCATCCACACCATCGAGTACTGCCTGG GCTGCATCTCCAACACTGCCTCCTACTTGCGGCTCTGGGCCCTCAGCCTCGCTCATGCGCAGCTGTCTGA GGTGCTTTGGACCATGGTGATCCACATCGGCCTGAGCGTGAAGAGCTTGGCGGGAGGTTTGGTGCTGTTC TTCTTCTTCACTGCCTTTGCCACCCTGACCGTGGCCATCCTCCTGATCATGGAGGGCCTCTCGGCCTTTC TCCACGCACTGCGCTTACACTGGGTTGAGTTCCAGAATAAATTCTACAGCGGGACCGGTTTCAAGTTCTT ACCCTTCTCCTTCGAGCATATTCGGGAAGGGAAGTTTGAAGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223769 representing NM_005177
Red=Cloning site Green=Tags(s) MGELFRSEEMTLAQLFLQSEAAYCCVSELGELGKVQFRDLNPDVNVFQRKFVNEVRRCEEMDRKLRFVEK EIRKANIPIMDTGENPEVPFPRDMIDLEANFEKIENELKEINTNQEALKRNFLELTELKFILRKTQQFFD EMADPDLLEESSSLLEPSEMGRGTPLRLGFVAGVINRERIPTFERMLWRVCRGNVFLRQAEIENPLEDPV TGDYVHKSVFIIFFQGDQLKNRVKKICEGFRASLYPCPETPQERKEMASGVNTRIDDLQMVLNQTEDHRQ RVLQAAAKNIRVWFIKVRKMKAIYHTLNLCNIDVTQKCLIAEVWCPVTDLDSIQFALRRGTEHSGSTVPS ILNRMQTNQTPPTYNKTNKFTYGFQNIVDAYGIGTYREINPAPYTIITFPFLFAVMFGDFGHGILMTLFA VWMVLRESRILSQKNENEMFSTVFSGRYIILLMGVFSMYTGLIYNDCFSKSLNIFGSSWSVRPMFTYNWT EETLRGNPVLQLNPALPGVFGGPYPFGIDPIWNIATNKLTFLNSFKMKMSVILGIIHMLFGVSLSLFNHI YFKKPLNIYFGFIPEIIFMTSLFGYLVILIFYKWTAYDAHTSENAPSLLIHFINMFLFSYPESGYSMLYS GQKGIQCFLVVVALLCVPWMLLFKPLVLRRQYLRRKHLGTLNFGGIRVGNGPTEEDAEIIQHDQLSTHSE DADEFDFGDTMVHQAIHTIEYCLGCISNTASYLRLWALSLAHAQLSEVLWTMVIHIGLSVKSLAGGLVLF FFFTAFATLTVAILLIMEGLSAFLHALRLHWVEFQNKFYSGTGFKFLPFSFEHIREGKFEE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005177 |
| ORF Size | 2493 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005177.5 |
| RefSeq Size | 4139 bp |
| RefSeq ORF | 2496 bp |
| Locus ID | 535 |
| UniProt ID | Q93050 |
| Domains | V_ATPase_sub_a |
| Protein Families | Transmembrane |
| Protein Pathways | Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection |
| Gene Summary | This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three A subunit proteins and the encoded protein is associated with clathrin-coated vesicles. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223769 | ATP6V0A1 (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal V0 subunit a1 (ATP6V0A1), transcript variant 3 |
CNY 6,088.00 |
|
| RC223769L3 | Lenti ORF clone of Human ATPase, H+ transporting, lysosomal V0 subunit a1 (ATP6V0A1), transcript variant 3, Myc-DDK-tagged |
CNY 10,640.00 |
|
| RC223769L4 | Lenti ORF clone of Human ATPase, H+ transporting, lysosomal V0 subunit a1 (ATP6V0A1), transcript variant 3, mGFP tagged |
CNY 10,640.00 |
|
| SC116892 | ATP6V0A1 (untagged)-Human ATPase, H+ transporting, lysosomal V0 subunit a1 (ATP6V0A1), transcript variant 3 |
CNY 6,096.00 |
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