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INPP5B (NM_005540) Human Tagged ORF Clone
CAT＃: RG223630
- TrueORF®
INPP5B (tGFP-tagged) - Human inositol polyphosphate-5-phosphatase, 75kDa (INPP5B), nuclear gene encoding mitochondrial protein
ORF Plasmid: DDK
"NM_005540" in other vectors (5)
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CNY 8,288.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 5PTase |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG223630 representing NM_005540
Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCAGTCTGTGGCAATCCAGGAGACGCTGGCTGAGGGGGAATACTGCGTCATCGCGGTGCAAGGTG TGCTGTGTGAGGGGGACAGCCGGCAGAGCCGCCTCCTGGGACTCGTGCGCTACCGCCTGGAGCACGGCGG CCAGGAACACGCTCTCTTCCTCTATACGCACCGGAGGATGGCCATTACCGGGGACGATGTCTCTCTGGAC CAGATAGTGCCAGTCTCGCGGGATTTTACGCTGGAAGAAGTGTCCCCAGATGGTGAACTCTACATCCTTG GCTCAGATGTGACCGTCCAGCTGGACACAGCAGAGCTTAGCCTCGTATTCCAACTGCCCTTTGGTTCACA AACCAGGATGTTCCTCCACGAAGTTGCCAGGGCCTGTCCAGGCTTCGATTCTGCGACCCGGGATCCTGAA TTCCTGTGGCTGTCTCGGTATAGGTGCGCAGAGCTGGAGCTGGAGATGCCAACGCCGCGCGGTTGTAACT CGGCCCTAGTTACCTGGCCAGGGTACGCGACAATTGGCGGAGGTGGTTCTAACTTTGATGGTTTGAGACC AAATGGGAAGGGAGTGCCTATGGACCAAAGCTCCAGGGGTCAAGATAAACCAGAAAGCTTGCAACCAAGA CAGAATAAATCCAAGTCCGAAATTACTGACATGGTTCGCTCCTCCACTATCACAGTGTCGGACAAGGCTC ATATTTTATCCATGCAGAAGTTTGGACTGCGAGATACAATTGTGAAATCACATCTACTACAGAAAGAAGA GGATTACACCTATATCCAGAACTTCAGGTTTTTTGCGGGAACATACAATGTAAATGGGCAGTCCCCCAAA GAATGCCTCCGGCTGTGGCTGAGCAATGGTATCCAGGCCCCAGATGTCTATTGTGTAGGGTTCCAGGAGC TTGATCTGAGTAAGGAAGCTTTTTTCTTTCACGATACCCCAAAGGAGGAAGAGTGGTTCAAAGCTGTGTC AGAGGGTCTTCATCCAGATGCCAAATATGCAAAGGTGAAGCTTATCCGACTGGTTGGGATTATGCTGCTG TTATATGTCAAACAGGAGCATGCAGCTTATATCTCAGAAGTGGAAGCCGAGACTGTGGGGACAGGAATCA TGGGGAGGATGGGCAACAAGGGAGGCGTGGCGATCAGGTTCCAGTTCCACAACACCAGCATCTGCGTTGT GAATTCTCACTTGGCAGCCCACATTGAAGAGTATGAGAGGAGGAACCAGGACTATAAGGACATTTGTTCT CGAATGCAGTTTTGTCAGCCTGACCCAAGCCTTCCCCCTCTCACCATCAGCAACCATGATGTGATCTTGT GGCTGGGGGACCTCAACTACAGGATAGAAGAGCTGGATGTGGAAAAAGTGAAAAAGCTCATCGAAGAGAA GGACTTTCAAATGCTGTATGCATATGATCAGCTGAAAATTCAGGTGGCCGCAAAGACTGTCTTTGAAGGC TTCACAGAGGGTGAGCTCACATTCCAGCCTACTTACAAGTATGATACGGGCTCTGACGACTGGGATACCA GTGAGAAGTGCCGTGCTCCTGCCTGGTGTGATCGGATTCTCTGGAAAGGGAAGAACATCACTCAGCTGAG TTACCAGAGCCACATGGCCCTGAAGACCAGTGACCACAAGCCTGTCAGCTCAGTGTTTGACATCGGGGTG AGGGTCGTAAATGACGAGCTTTACCGGAAGACACTGGAGGAAATTGTTCGCTCCCTGGATAAGATGGAAA ATGCCAACATTCCTTCTGTGTCCCTGTCCAAGCGAGAGTTCTGTTTTCAGAATGTGAAGTACATGCAATT GAAAGTAGAATCCTTTACAATTCATAATGGACAAGTACCCTGTCATTTTGAATTCATCAACAAGCCTGAT GAAGAGTCTTACTGTAAGCAGTGGCTGAATGCCAACCCCAGCAGAGGCTTCCTCCTGCCAGATTCTGATG TTGAGATTGACTTGGAGCTCTTCGTAAATAAGATGACAGCTACAAAGCTCAACTCGGGTGAAGACAAAAT TGAGGACATTCTGGTTCTGCACTTGGACAGGGGAAAGGATTACTTTTTGTCTGTGTCTGGGAACTACCTG CCCAGCTGTTTTGGGTCTCCCATTCATACACTGTGTTACATGAGAGAGCCAATCTTGGACCTACCACTTG AAACCATTAGTGAGCTGACTCTGATGCCAGTATGGACTGGAGATGATGGGAGCCAGTTGGATAGCCCCAT GGAAATCCCCAAAGAGCTCTGGATGATGGTTGATTACCTGTACCGAAATGCTGTCCAGCAGGAAGATCTG TTTCAGCAACCAGGCCTGAGGTCAGAATTTGAACATATCAGGGACTGCTTGGATACTGGAATGATTGATA ACCTCTCTGCCAGCAATCATTCTGTAGCCGAAGCCCTGCTGCTTTTCCTGGAGAGCCTTCCAGAGCCTGT CATCTGTTACAGCACCTACCATAACTGCTTGGAGTGTTCTGGCAACTACACAGCAAGCAAACAGGTCATT TCTACTCTCCCCATATTCCACAAAAATGTCTTCCACTACTTGATGGCGTTTTTGCGAGAACTGCTGAAAA ATTCAGCAAAAAATCATTTGGATGAGAATATTCTAGCTAGCATATTTGGCAGCTTATTGCTTCGAAACCC AGCTGGTCACCAAAAGCTTGATATGACAGAGAAGAAGAAGGCTCAAGAATTTATTCACCAGTTCCTCTGC AACCCACTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005540 |
| ORF Size | 2739 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005540.3 |
| RefSeq Size | 4469 bp |
| RefSeq ORF | 2742 bp |
| Locus ID | 3633 |
| UniProt ID | P32019 |
| Protein Families | Druggable Genome |
| Protein Pathways | Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system |
| Gene Summary | This gene encodes a member of a family of inositol polyphosphate-5-phosphatases. These enzymes function in the regulation of calcium signaling by inactivating inositol phosphates. The encoded protein is localized to the cytosol and mitochondria, and associates with membranes through an isoprenyl modification near the C-terminus. Alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Jul 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223630 | INPP5B (Myc-DDK-tagged)-Human inositol polyphosphate-5-phosphatase, 75kDa (INPP5B), nuclear gene encoding mitochondrial protein |
CNY 6,688.00 |
|
| RC223630L3 | Lenti-ORF clone of INPP5B (Myc-DDK-tagged)-Human inositol polyphosphate-5-phosphatase, 75kDa (INPP5B), nuclear gene encoding mitochondrial protein |
CNY 11,500.00 |
|
| RC223630L4 | Lenti-ORF clone of INPP5B (mGFP-tagged)-Human inositol polyphosphate-5-phosphatase, 75kDa (INPP5B), nuclear gene encoding mitochondrial protein |
CNY 11,500.00 |
|
| SC125851 | INPP5B (untagged)-Human inositol polyphosphate-5-phosphatase, 75kDa (INPP5B), nuclear gene encoding mitochondrial protein |
CNY 5,488.00 |
|
| SC318386 | INPP5B (untagged)-Human inositol polyphosphate-5-phosphatase, 75kDa (INPP5B), nuclear gene encoding mitochondrial protein |
CNY 17,580.00 |
