PPA2 (NM_001034191) Human Tagged ORF Clone

CAT#: RG223425

  • TrueORF®

PPA2 (tGFP-tagged) - Human pyrophosphatase (inorganic) 2 (PPA2), nuclear gene encoding mitochondrial protein, transcript variant 5

ORF Plasmid: DDK tGFP



  "NM_001034191" in other vectors (4)

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CNY 4,370.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


PPA2 mouse monoclonal antibody, clone OTI7C1 (formerly 7C1)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms FLJ20459; HSPC124; inorganic pyrophosphatase 2; MGC49850; pyrophosphatase (inorganic) 2; SID6-306
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG223425 representing NM_001034191
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGCGCTGCTGCGGCTGCTGCGCACGGGTGCCCCAGCCGCTGCGTGCCTGCGGTTGGGGACCAGTG
CAGGGACCGGGTCGCGCCGTGCTATGGCCCTGTACCACACTGAGGAGCGCGGCCAGCCCTGCTCGCAGAA
TTACCGCCTCTTCTTTAAGAATGTAACTGGTCACTACATTTCCCCCTTTCATGATATTCCTCTGAAGGTG
AACTCTAAAGAGGAAAATGGCATTCCTATGAAGAAAGCACGAAATGATGAATATGAGATTGCCACCAAGG
AGCCAATGAATCCCATTAAACAATATGTAAAGGATGGAAAGCTACGCTATGTGGCGAATATCTTCCCTTA
CAAGGGTTATATATGGAATTATGGTACCCTCCCTCAGACTTGGGAAGATCCCCATGAAAAAGATAAGAGC
ACGAACTGCTTTGGAGATAATGATCCTATTGATGTTTGCGAAATAGGCTCAAAGATTCTTTCTTGTGGAG
AAGTTATTCATGTGAAGATCCTTGGAATTTTGGCTCTTATTGATGAAGGTGAAACAGATTGGAAATTAAT
TGCTATCAATGCGAATGATCCTGAAGCCTCAAAGTTTCATGATATTGATGATGTTAAGAAGTTCAAACCG
GGTTACCTGGAAGCTACTCTTAATTGGTTTAGATTATATAAGGTACCAGATGGAAAACCAGAAAACCAGT
TTGCTTTTAATGGAGAATTCAAAAACAAGGCTTTTGCTCTTGAAGTTATTAAATCCACTCATCAATGTTG
GAAAGCATTGCTTATGAAGAAGTGTAATGGAGGAGCTATAAATTGCACAAACGTGCAGATATCTGATAGC
CCTTTCCGTTGCACTCAAGAGGAAGCAAGATCATTAGTTGAATCGGTATCATCTTCACCAAATAAAGAAA
GTAATGAAGAAGAGCAAGTGTGGCACTTCCTTGGCAAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG223425 representing NM_001034191
Red=Cloning site Green=Tags(s)

MSALLRLLRTGAPAAACLRLGTSAGTGSRRAMALYHTEERGQPCSQNYRLFFKNVTGHYISPFHDIPLKV
NSKEENGIPMKKARNDEYEIATKEPMNPIKQYVKDGKLRYVANIFPYKGYIWNYGTLPQTWEDPHEKDKS
TNCFGDNDPIDVCEIGSKILSCGEVIHVKILGILALIDEGETDWKLIAINANDPEASKFHDIDDVKKFKP
GYLEATLNWFRLYKVPDGKPENQFAFNGEFKNKAFALEVIKSTHQCWKALLMKKCNGGAINCTNVQISDS
PFRCTQEEARSLVESVSSSPNKESNEEEQVWHFLGK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001034191
ORF Size 948 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001034191.1, NP_001029363.1
RefSeq Size 1628 bp
RefSeq ORF 950 bp
Locus ID 27068
Protein Pathways Oxidative phosphorylation
Gene Summary The protein encoded by this gene is localized to the mitochondrion, is highly similar to members of the inorganic pyrophosphatase (PPase) family, and contains the signature sequence essential for the catalytic activity of PPase. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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