AP2S1 (NM_021575) Human Tagged ORF Clone
CAT#: RG223424
- TrueORF®
AP2S1 (tGFP-tagged) - Human adaptor-related protein complex 2, sigma 1 subunit (AP2S1), transcript variant AP17delta
ORF Plasmid: DDK
"NM_021575" in other vectors (4)
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CNY 2,920.00
CNY 4,370.00
Cited in 1 publication. |
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AP17; CLAPS2; FBH3; FBHOk; HHC3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG223424 representing NM_021575
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCCGCTTTATCCTCATCCAGAACCGGGCAGGCAAGACGCGCCTGGCCAAGTGGTACATGCAGTTTG ATGATGATGAGAAACAGAAGCTGATCGAGGAGGTGCATGCCGTGGTCACCGTCCGAGACGCCAAACACAC CAACTTTGTGGAGTTCCGGAACTTTAAGATCATTTACCGCCGCTATGCTGGCCTCTACTTCTGCATCTGT GTGGATGTCAATGACAACAACCTGGCTTACCTGGAGGCCATTCACAACTTCGTGGAGGTCTTAAACGAAT ATTTCCACAATGTCTGTGAACTGGACCTGGTGTTCAACTTCTACAAGGTTTACACGGTCGTGGACGAGAT GTTCCTGGCTGGCGAAATCCGAGAGACCAGCCAGACGAAGGTGCTGAAACAGCTGCTGATGCTACAGTCC CTGGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223424 representing NM_021575
Red=Cloning site Green=Tags(s) MIRFILIQNRAGKTRLAKWYMQFDDDEKQKLIEEVHAVVTVRDAKHTNFVEFRNFKIIYRRYAGLYFCIC VDVNDNNLAYLEAIHNFVEVLNEYFHNVCELDLVFNFYKVYTVVDEMFLAGEIRETSQTKVLKQLLMLQS LE TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_021575 |
ORF Size | 426 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021575.2, NP_067586.1 |
RefSeq Size | 776 bp |
RefSeq ORF | 315 bp |
Locus ID | 1175 |
UniProt ID | P53680 |
Domains | Clat_adaptor_s |
Protein Pathways | Endocytosis, Huntington's disease |
Gene Summary | One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Targeting F-Box Protein Fbxo3 Attenuates Lung Injury Induced by Ischemia-Reperfusion in Rats
,Hung, KY;Liao, WI;Pao, HP;Wu, SY;Huang, KL;Chu, SJ;,
Front Pharmacol
,PubMed ID 31178737
[AP2S1]
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Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC223424 | AP2S1 (Myc-DDK-tagged)-Human adaptor-related protein complex 2, sigma 1 subunit (AP2S1), transcript variant AP17delta |
CNY 3,990.00 |
|
RC223424L3 | Lenti-ORF clone of AP2S1 (Myc-DDK-tagged)-Human adaptor-related protein complex 2, sigma 1 subunit (AP2S1), transcript variant AP17delta |
CNY 5,890.00 |
|
RC223424L4 | Lenti-ORF clone of AP2S1 (mGFP-tagged)-Human adaptor-related protein complex 2, sigma 1 subunit (AP2S1), transcript variant AP17delta |
CNY 5,890.00 |
|
SC109070 | AP2S1 (untagged)-Human adaptor-related protein complex 2, sigma 1 subunit (AP2S1), transcript variant AP17delta |
CNY 3,990.00 |