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RNF14 (NM_183400) Human Tagged ORF Clone
CAT＃: RG223362
- TrueORF®
RNF14 (tGFP-tagged) - Human ring finger protein 14 (RNF14), transcript variant 4
ORF Plasmid: DDK
"NM_183400" in other vectors (6)
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CNY 4,370.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ARA54; HFB30; HRIHFB2038; TRIAD2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG223362 representing NM_183400.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCGTCAGAAGATCGAGAAGCTCAGGAGGATGAATTGCTGGCCCTGGCAAGTATTTACGATGGAGAT GAATTTAGAAAAGCAGAGTCTGTCCAAGGTGGAGAAACCAGGATCTATTTGGATTTGCCACAGAATTTC AAGATATTTGTGAGCGGCAATTCAAATGAGTGTCTCCAGAATAGTGGCTTTGAATACACCATTTGCTTT CTGCCTCCACTTGTGCTGAACTTTGAACTGCCACCAGATTATCCATCCTCTTCCCCACCTTCATTCACA CTTAGTGGCAAATGGCTGTCACCAACTCAGCTATCTGCTCTATGCAAGCACTTAGACAACCTATGGGAA GAACACCGTGGCAGCGTGGTCCTGTTTGCCTGGATGCAATTTCTTAAGGAAGAGACCCTAGCATACTTG AATATTGTCTCTCCTTTTGAGCTCAAGATTGGTTCTCAGAAAAAAGTGCAGAGAAGGACAGCTCAAGCT TCTCCCAACACAGAGCTAGATTTTGGAGGAGCTGCTGGATCTGATGTAGACCAAGAGGAAATTGTGGAT GAGAGAGCAGTGCAGGATGTGGAATCACTGTCAAATCTGATCCAGGAAATCTTGGACTTTGATCAAGCT CAGCAGATAAAATGCTTTAATAGTAAATTGTTCCTGTGCAGTATCTGTTTCTGTGAGAAGCTGGGTAGT GAATGCATGTACTTCTTGGAGTGCAGGCATGTGTACTGCAAAGCCTGTCTGAAGGACTACTTTGAAATC CAGATCAGAGATGGCCAGGTTCAATGCCTCAACTGCCCAGAACCAAAGTGCCCTTCGGTGGCCACTCCT GGTCAGGTCAAAGAGTTAGTGGAAGCAGAGTTATTTGCCCGTTATGACCGCCTTCTCCTCCAGTCCTCC TTGGACCTGATGGCAGATGTGGTGTACTGCCCCCGGCCGTGCTGCCAGCTGCCTGTGATGCAGGAACCT GGCTGCACCATGGGTATCTGCTCCAGCTGCAATTTTGCCTTCTGTACTTTGTGCAGGTTGACCTACCAT GGGGTCTCCCCATGTAAGGTGACTGCAGAGAAATTAATGGACTTACGAAATGAATACCTGCAAGCGGAT GAGGCTAATAAAAGACTTTTGGATCAAAGGTATGGTAAGAGAGTGATTCAGAAGGCACTGGAAGAGATG GAAAGTAAGGAGTGGCTAGAGAAGAACTCAAAGAGCTGCCCATGTTGTGGAACTCCCATAGAGAAATTA GACGGATGTAACAAGATGACATGTACTGGCTGTATGCAATATTTCTGTTGGATTTGCATGGGTTCTCTC TCTAGAGCAAACCCTTACAAACATTTCAATGACCCTGGTTCACCATGTTTTAACCGGCTGTTTTATGCT GTGGATGTTGACGACGATATTTGGGAAGATGAGGTAGAAGAC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG223362
Blue=ORF Red=Cloning site Green=Tag(s) MSSEDREAQEDELLALASIYDGDEFRKAESVQGGETRIYLDLPQNFKIFVSGNSNECLQNSGFEYTICF LPPLVLNFELPPDYPSSSPPSFTLSGKWLSPTQLSALCKHLDNLWEEHRGSVVLFAWMQFLKEETLAYL NIVSPFELKIGSQKKVQRRTAQASPNTELDFGGAAGSDVDQEEIVDERAVQDVESLSNLIQEILDFDQA QQIKCFNSKLFLCSICFCEKLGSECMYFLECRHVYCKACLKDYFEIQIRDGQVQCLNCPEPKCPSVATP GQVKELVEAELFARYDRLLLQSSLDLMADVVYCPRPCCQLPVMQEPGCTMGICSSCNFAFCTLCRLTYH GVSPCKVTAEKLMDLRNEYLQADEANKRLLDQRYGKRVIQKALEEMESKEWLEKNSKSCPCCGTPIEKL DGCNKMTCTGCMQYFCWICMGSLSRANPYKHFNDPGSPCFNRLFYAVDVDDDIWEDEVED SGPTRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLL SHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGF PEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGP MFAFRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_183400 |
| ORF Size | 1422 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_183400.3 |
| RefSeq Size | 2970 bp |
| RefSeq ORF | 1425 bp |
| Locus ID | 9604 |
| UniProt ID | Q9UBS8 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 53.8 kDa |
| Gene Summary | The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Six alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq, Jan 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223362 | RNF14 (Myc-DDK-tagged)-Human ring finger protein 14 (RNF14), transcript variant 4 |
CNY 3,656.00 |
|
| RC223362L1 | Lenti ORF clone of Human ring finger protein 14 (RNF14), transcript variant 4, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC223362L2 | Lenti ORF clone of Human ring finger protein 14 (RNF14), transcript variant 4, mGFP tagged |
CNY 5,890.00 |
|
| RC223362L3 | Lenti ORF clone of Human ring finger protein 14 (RNF14), transcript variant 4, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC223362L4 | Lenti ORF clone of Human ring finger protein 14 (RNF14), transcript variant 4, mGFP tagged |
CNY 5,890.00 |
|
| SC310349 | RNF14 (untagged)-Human ring finger protein 14 (RNF14), transcript variant 4 |
CNY 7,510.00 |
