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ATP2B3 (NM_021949) Human Tagged ORF Clone
CAT＃: RG223162
- TrueORF®
ATP2B3 (tGFP-tagged) - Human ATPase, Ca++ transporting, plasma membrane 3 (ATP2B3), transcript variant 1
ORF Plasmid: DDK
"NM_021949" in other vectors (4)
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CNY 12,830.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CFAP39; CLA2; OPCA; PMCA3; PMCA3a; SCAX1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG223162 representing NM_021949
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGACATGGCCAATAGTTCCATCGAGTTCCACCCCAAGCCCCAGCAGCAGCGGGATGTCCCCCAGG CTGGAGGCTTTGGGTGCACGCTGGCGGAGCTGCGCACCCTCATGGAGCTGCGAGGGGCCGAGGCGCTGCA GAAGATCGAGGAGGCCTACGGGGATGTCAGCGGGCTCTGCCGGAGGCTGAAGACCTCACCCACAGAGGGC CTGGCGGACAACACCAATGACCTGGAGAAGCGCAGGCAGATCTACGGGCAGAACTTCATCCCCCCAAAGC AACCCAAGACCTTCCTGCAGCTGGTGTGGGAGGCCCTGCAGGACGTGACCCTCATCATCCTGGAGGTGGC TGCCATCGTCTCTCTGGGCCTCTCGTTCTATGCGCCGCCAGGAGAGGAGAGTGAAGCCTGTGGGAATGTG TCGGGAGGCGCAGAAGATGAGGGCGAGGCCGAAGCTGGCTGGATCGAGGGGGCTGCCATCCTGCTGTCCG TCATCTGTGTGGTGCTGGTCACGGCCTTCAATGACTGGAGCAAGGAGAAGCAGTTCCGAGGCCTGCAGAG CCGAATTGAGCAGGAGCAGAAGTTCACGGTCATCCGGAACGGGCAGCTCCTCCAGGTCCCCGTGGCTGCG CTGGTGGTGGGGGACATTGCCCAGGTCAAGTACGGCGACCTGCTGCCAGCCGACGGCGTGCTCATCCAGG CCAATGACCTCAAGATCGACGAGAGCTCCCTGACGGGCGAGTCTGACCACGTGCGCAAGTCAGCTGACAA AGATCCCATGCTGCTCTCAGGCACTCATGTCATGGAAGGTTCTGGAAGAATGGTGGTGACCGCCGTTGGC GTGAATTCCCAGACAGGCATCATCTTCACGCTGCTTGGAGCTGGCGGAGAGGAGGAAGAGAAGAAAGATA AGAAAGGCAAGCAGCAGGATGGGGCCATGGAGAGTAGCCAGACCAAAGCTAAGAAGCAGGATGGTGCAGT GGCCATGGAGATGCAGCCCCTGAAGAGCGCGGAGGGTGGGGAGATGGAGGAGCGGGAGAAGAAGAAAGCC AACGCACCCAAAAAGGAGAAGTCTGTCCTTCAGGGGAAGCTCACAAAGCTAGCCGTGCAGATCGGGAAAG CAGGGCTGGTGATGTCTGCCATCACCGTCATCATCCTGGTCCTCTACTTTGTGATTGAGACGTTTGTCGT GGAAGGCCGGACATGGCTGGCAGAGTGCACGCCGGTCTATGTACAATACTTCGTGAAGTTCTTCATCATT GGTGTCACTGTGCTGGTCGTGGCTGTCCCAGAGGGCCTGCCTCTTGCTGTCACCATCTCCTTAGCTTACT CTGTCAAGAAAATGATGAAAGACAACAACCTGGTGCGCCACCTGGATGCCTGCGAGACCATGGGCAACGC CACAGCCATCTGCTCCGACAAGACGGGCACGCTCACCACCAACCGTATGACCGTGGTCCAGTCCTACCTA GGAGACACCCACTACAAAGAGATTCCGGCCCCCAGCGCCCTGACCCCTAAGATCCTCGACCTCCTGGTCC ATGCCATCTCCATCAACAGTGCCTATACCACCAAAATACTACCTCCTGAGAAGGAAGGCGCCCTCCCACG CCAGGTGGGCAATAAGACGGAGTGCGCCCTGCTGGGCTTCGTCTTGGACCTGAAGCGGGACTTCCAGCCC GTGCGCGAGCAGATCCCGGAAGACAAGCTTTACAAAGTGTACACCTTCAACTCGGTCCGCAAGTCCATGA GCACAGTCATCCGCATGCCCGACGGTGGCTTCCGCCTCTTCAGCAAGGGGGCCTCAGAGATCCTCTTGAA AAAGTGCACCAACATCTTGAACAGCAATGGCGAACTCCGGGGCTTTCGGCCTCGGGACCGGGACGACATG GTGAGGAAGATCATCGAGCCGATGGCTTGCGATGGCCTCCGCACCATCTGCATCGCCTACCGGGACTTCT CTGCAGGCCAGGAGCCCGACTGGGACAACGAGAATGAGGTCGTGGGTGACCTCACCTGCATAGCTGTCGT GGGCATTGAGGACCCTGTGCGGCCCGAGGTCCCTGAAGCTATCCGAAAATGCCAGCGTGCTGGCATCACA GTCCGCATGGTGACTGGGGACAACATCAACACGGCCCGGGCCATCGCAGCCAAATGCGGCATCATCCAGC CCGGGGAGGACTTCCTGTGCCTAGAAGGGAAGGAGTTCAACCGGCGGATCCGCAATGAGAAAGGCGAGAT AGAACAGGAGCGGCTGGACAAGGTGTGGCCCAAGCTGAGGGTGCTGGCCCGGTCGTCTCCCACCGACAAG CACACACTGGTCAAAGGGATTATCGACAGCACCACTGGTGAGCAGCGGCAGGTGGTGGCTGTGACAGGGG ATGGCACCAACGATGGGCCGGCCCTCAAGAAGGCGGACGTGGGCTTCGCCATGGGCATCGCAGGGACCGA CGTGGCCAAGGAGGCCTCCGACATCATCCTGACCGATGACAACTTCACCAGCATCGTCAAGGCAGTCATG TGGGGCCGTAACGTCTATGACAGCATCTCCAAGTTCCTGCAGTTTCAACTGACGGTCAATGTGGTGGCTG TGATCGTGGCCTTCACAGGTGCCTGCATTACTCAGGACTCTCCTCTCAAAGCCGTGCAGATGTTGTGGGT GAACTTGATCATGGACACATTTGCCTCTCTGGCCCTGGCGACGGAGCCACCCACAGAGTCGCTGCTGCTG CGGAAGCCGTACGGCCGCGACAAGCCCCTCATCTCCCGCACCATGATGAAGAACATTCTGGGCCACGCCG TGTACCAGCTCGCCATCATCTTCACCCTGCTGTTTGTCGGGGAGCTCTTCTTCGACATCGACAGCGGGAG GAATGCGCCCCTGCACTCGCCACCCTCAGAGCACTACACCATCATCTTCAACACGTTCGTCATGATGCAG CTCTTTAACGAGATCAACGCCCGCAAGATCCACGGCGAGAGGAACGTGTTCGACGGCATCTTCAGCAACC CCATCTTCTGCACCATCGTTTTGGGCACTTTCGGGATTCAGATTGTCATCGTCCAGTTTGGCGGGAAGCC CTTCAGCTGCTCCCCACTATCCACAGAACAGTGGCTCTGGTGCCTGTTTGTTGGTGTTGGGGAGCTGGTC TGGGGACAGGTCATTGCCACCATCCCCACCAGCCAGCTCAAGTGCCTGAAGGAAGCCGGGCACGGGCCCG GGAAGGACGAGATGACCGACGAGGAGCTGGCCGAAGGCGAGGAAGAGATCGACCATGCCGAGCGGGAGCT CCGCAGGGGCCAGATCCTCTGGTTCCGGGGCCTGAACCGGATTCAGACGCAGATGGAGGTAGTGAGTACC TTCAAGAGAAGCGGTTCAGTTCAGGGTGCTGTGCGCCGGCGGTCTTCGGTCCTCAGCCAGCTTCATGACG TAACCAATCTTTCTACCCCTACTCACGCAATTCTCTCTGCTGCCAATCCTACCAGTGCTGCTGGGAATCC GGGTGGTGAAAGCGTTCCG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223162 representing NM_021949
Red=Cloning site Green=Tags(s) MGDMANSSIEFHPKPQQQRDVPQAGGFGCTLAELRTLMELRGAEALQKIEEAYGDVSGLCRRLKTSPTEG LADNTNDLEKRRQIYGQNFIPPKQPKTFLQLVWEALQDVTLIILEVAAIVSLGLSFYAPPGEESEACGNV SGGAEDEGEAEAGWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVIRNGQLLQVPVAA LVVGDIAQVKYGDLLPADGVLIQANDLKIDESSLTGESDHVRKSADKDPMLLSGTHVMEGSGRMVVTAVG VNSQTGIIFTLLGAGGEEEEKKDKKGKQQDGAMESSQTKAKKQDGAVAMEMQPLKSAEGGEMEEREKKKA NAPKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILVLYFVIETFVVEGRTWLAECTPVYVQYFVKFFII GVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQSYL GDTHYKEIPAPSALTPKILDLLVHAISINSAYTTKILPPEKEGALPRQVGNKTECALLGFVLDLKRDFQP VREQIPEDKLYKVYTFNSVRKSMSTVIRMPDGGFRLFSKGASEILLKKCTNILNSNGELRGFRPRDRDDM VRKIIEPMACDGLRTICIAYRDFSAGQEPDWDNENEVVGDLTCIAVVGIEDPVRPEVPEAIRKCQRAGIT VRMVTGDNINTARAIAAKCGIIQPGEDFLCLEGKEFNRRIRNEKGEIEQERLDKVWPKLRVLARSSPTDK HTLVKGIIDSTTGEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVM WGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTESLLL RKPYGRDKPLISRTMMKNILGHAVYQLAIIFTLLFVGELFFDIDSGRNAPLHSPPSEHYTIIFNTFVMMQ LFNEINARKIHGERNVFDGIFSNPIFCTIVLGTFGIQIVIVQFGGKPFSCSPLSTEQWLWCLFVGVGELV WGQVIATIPTSQLKCLKEAGHGPGKDEMTDEELAEGEEEIDHAERELRRGQILWFRGLNRIQTQMEVVST FKRSGSVQGAVRRRSSVLSQLHDVTNLSTPTHAILSAANPTSAAGNPGGESVP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021949 |
| ORF Size | 3519 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021949.3, NP_068768.2 |
| RefSeq Size | 6574 bp |
| RefSeq ORF | 3522 bp |
| Locus ID | 492 |
| UniProt ID | Q16720 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Calcium signaling pathway |
| Gene Summary | The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 3. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223162 | ATP2B3 (Myc-DDK-tagged)-Human ATPase, Ca++ transporting, plasma membrane 3 (ATP2B3), transcript variant 1 |
CNY 11,690.00 |
|
| RC223162L3 | Lenti-ORF clone of ATP2B3 (Myc-DDK-tagged)-Human ATPase, Ca++ transporting, plasma membrane 3 (ATP2B3), transcript variant 1 |
CNY 13,590.00 |
|
| RC223162L4 | Lenti-ORF clone of ATP2B3 (mGFP-tagged)-Human ATPase, Ca++ transporting, plasma membrane 3 (ATP2B3), transcript variant 1 |
CNY 13,590.00 |
|
| SC304976 | ATP2B3 (untagged)-Human ATPase, Ca++ transporting, plasma membrane 3 (ATP2B3), transcript variant 1 |
CNY 22,520.00 |
