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RIF1 (NM_018151) Human Tagged ORF Clone
CAT＃: RG223158
- TrueORF®
RIF1 (tGFP-tagged) - Human RAP1 interacting factor homolog (yeast) (RIF1), transcript variant 1
ORF Plasmid: DDK
"NM_018151" in other vectors (2)
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CNY 26,080.00
货期*
现货
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG223158 representing NM_018151
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGCCAGGGGTCAGAGCCCCCTCGCGCCGCTGTTGGAGACTTTGGAAGACCCTTCTGCCTCCCATG GAGGGCAGACTGACGCTTACCTGACTCTGACCAGTCGTATGACTGGAGAAGAAGGAAAAGAAGTAATTAC AGAAATTGAGAAAAAACTTCCTCGGCTGTACAAAGTTTTAAAGACTCACATTTCCAGTCAAAACTCGGAG CTGAGTAGTGCTGCTCTACAAGCCCTGGGGTTTTGCTTATATAATCCCAAAATTACCTCAGAATTATCAG AGGCAAATGCTCTAGAATTGCTTTCAAAATTGAATGATACCATTAAGAATTCAGACAAAAATGTACGTAC TAGAGCACTTTGGGTGATATCTAAGCAGACATTTCCCTCTGAAGTGGTTGGCAAAATGGTATCCAGTATA ATTGATTCATTAGAAATACTGTTTAACAAAGGAGAGACGCATTCTGCTGTTGTTGATTTTGAAGCATTAA ATGTTATCGTAAGGCTAATTGAACAAGCCCCAATTCAAATGGGAGAAGAGGCAGTGAGGTGGGCAAAACT GGTCATACCTTTAGTGGTTCATTCAGCACAAAAGGTACATTTGCGGGGAGCAACTGCTCTGGAGATGGGA ATGCCATTATTGCTTCAGAAACAGCAAGAAATAGCATCTATTACGGAGCAGCTTATGACTACTAAATTAA TCTCAGAACTTCAGAAGCTATTTATGAGTAAAAATGAGACTTACGTGTTAAAATTATGGCCTTTGTTTGT CAAACTACTTGGAAGGACCTTGCATCGAAGTGGGAGTTTCATCAATTCTCTCTTGCAACTAGAAGAACTT GGATTTCGTAGTGGAGCACCCATGATTAAAAAGATAGCTTTTATTGCTTGGAAGAGTTTAATAGATAATT TTGCTTTAAATCCAGATATACTATGTAGTGCAAAAAGACTCAAGTTGTTAATGCAGCCTTTGAGTTCCAT CCATGTGAGAACAGAAACTCTAGCATTAACAAAACTAGAAGTCTGGTGGTATTTACTGATGAGACTTGGA CCTCATCTTCCTGCTAATTTTGAACAGGTTTGTGTGCCTCTGATTCAAAGTACAATAAGCATTGATTCTA ATGCCTCACCTCAGGGCAATTCGTGTCATGTAGCTACATCTCCAGGTTTAAATCCTATGACTCCTGTACA CAAAGGTGCTTCCTCCCCGTACGGAGCCCCGGGAACTCCCCGAATGAACCTGAGTTCGAATTTAGGTGGA ATGGCCACAATCCCATCCATTCAACTTTTGGGACTTGAAATGTTGCTTCATTTCTTGTTGGGTCCAGAAG CCTTGAGTTTTGCTAAGCAAAATAAACTTGTGCTGAGCTTAGAGCCATTGGAACATCCGTTAATCAGCAG CCCTTCCTTTTTTTCCAAACATGCAAATACACTTATCACTGCTGTTCATGATAGCTTTGTTGCAGTTGGA AAAGATGCCCCCGATGTGGTTGTCAGTGCTATCTGGAAGGAGCTAATTAGCTTGGTGAAGTCAGTTACTG AATCAGGTAACAAAAAAGAGAAACCAGGTTCTGAAGTTTTGACTCTCTTATTAAAGTCTTTGGAAAGCAT AGTAAAGTCTGAAGTATTTCCTGTATCAAAAACGCTGGTCCTCATGGAAATTACAATTAAAGGACTTCCT CAGAAAGTATTAGGTTCACCAGCATATCAGGTTGCTAATATGGATATTCTTAATGGAACTCCAGCTTTGT TCTTAATTCAATTAATTTTCAACAATTTCTTGGAATGTGGTGTATCAGATGAAAGGTTCTTTCTCAGTTT GGAATCACTTGTAGGCTGTGTTCTTTCTGGTCCAACTTCACCACTAGCTTTCAGTGACTCAGTTTTAAAT GTTATTAATCAAAATGCAAAGCAGTTGGAAAATAAGGAGCATCTCTGGAAAATGTGGAGTGTTATAGTCA CCCCATTAACTGAATTGATTAATCAGACCAATGAAGTAAATCAAGGTGATGCCTTAGAACATAATTTTAG TGCCATCTATGGTGCATTGACTTTACCAGTAAACCACATTTTTTCAGAACAGAGATTTCCAGTGGCCACC ATGAAGACTTTGCTTAGAACTTGGTCAGAATTATATAGAGCATTTGCTCGTTGTGCTGCTTTGGTGGCAA CAGCAGAAGAGAACTTGTGCTGTGAGGAACTTTCTTCCAAGATAATGTCCAGTTTGGAAGATGAAGGCTT TTCTAATTTGTTGTTCGTGGATAGAATTATTTATATTATTACTGTAATGGTTGATTGCATTGACTTCTCA CCATATAATATTAAATATCAGCCCAAAGTTAAATCACCACAGAGACCTTCAGATTGGTCCAAAAAGAAGA ATGAGCCCCTAGGGAAATTGACTTCTTTATTTAAACTTATTGTGAAAGTGATCTATTCTTTCCACACACT GAGCTTCAAGGAAGCACATTCTGATACCCTCTTCACTATTGGCAACTCAATCACCGGCATTATTTCCAGT GTACTTGGGCATATTTCTTTGCCTTCTATGATCCGAAAAATATTTGCAACTTTAACAAGACCTCTGGCAT TATTTTATGAAAACTCAAAGCTTGATGAAGTTCCTAAAGTATATAGTTGTCTGAACAACAAGTTAGAAAA GCTACTGGGAGAAATTATTGCTTGTCTGCAATTCAGCTACACCGGAACTTATGATAGTGAACTTCTTGAA CAACTCTCCCCACTATTATGCATAATATTTCTGCACAAGAATAAACAGATTCGAAAACAGAGTGCTCAGT TCTGGAATGCCACTTTTGCCAAAGTGATGATGTTGGTTTATCCTGAAGAGTTAAAACCAGTACTAACACA AGCCAAACAAAAATTTCTGCTCCTGTTGCCTGGTTTGGAAACTGTTGAAATGATGGAGGAATCCAGTGGA CCATATTCTGATGGAACAGAAAATTCACAACTAAATGTGAAGATAAGTGGCATGGAGAGAAAATCAAATG GAAAAAGAGATTCATTTTTGGCACAAACAAAGAATAAAAAAGAAAATATGAAACCAGCAGCCAAACTGAA ACTTGAATCTTCGTCTTTAAAAGTAAAGGGTGAAATTCTTTTGGAAGAGGAAAAGTCTACTGACTTTGTG TTTATACCTCCAGAAGGAAAAGATGCAAAGGAAAGAATATTAACTGATCATCAAAAAGAAGTTCTCAAAA CAAAGCGGTGTGATATTCCTGCCATGTATAATAATCTGGATGTTTCCCAAGATACCTTATTTACTCAGTA TAGTCAGGAAGAGCCTATGGAAATTCCTACTTTAACCAGAAAACCAAAGGAGGATTCTAAGATGATGATT ACGGAGGAGCAAATGGACAGTGACATTGTCATTCCTCAAGATGTCACGGAAGACTGTGGTATGGCTGAAC ATCTTGAAAAGTCCTCCCTTTCGAATAATGAGTGTGGTTCTCTTGACAAAACCAGTCCAGAAATGTCAAA CAGTAATAATGATGAAAGAAAAAAAGCTTTAATTTCATCAAGGAAAACATCAACTGAATGTGCATCTAGT ACAGAAAATTCTTTCGTTGTCAGCAGTAGTTCAGTTTCTAATACCACTGTTGCTGGAACTCCCCCATACC CTACAAGTCGGAGGCAAACCTTTATTACTTTGGAGAAGTTTGATGGTTCAGAAAATAGACCTTTTAGTCC ATCCCCCTTGAATAATATTTCATCAACTGTTACAGTGAAAAATAACCAGGAAACCATGATTAAAACAGAT TTTCTACCAAAAGCAAAGCAAAGAGAAGGGACTTTTTCAAAATCTGATTCTGAAAAAATAGTGAATGGAA CTAAGAGATCAAGCCGGAGAGCTGGTAAAGCTGAACAAACAGGGAATAAAAGGTCTAAGCCCTTAATGAG ATCTGAGCCGGAGAAAAATACTGAGGAATCTGTTGAAGGCATTGTAGTCTTAGAAAATAACCCACCTGGT TTGCTTAATCAAACAGAATGTGTGTCAGATAATCAGGTTCATCTTTCTGAATCTACAATGGAGCATGACA ATACAAAGCTTAAAGCAGCAACAGTGGAAAATGCTGTATTATTGGAAACTAATACTGTAGAGGAGAAAAA TGTAGAAATTAATTTGGAATCCAAAGAGAATACACCCCCAGTAGTAATATCAGCAGATCAAATGGTAAAT GAGGATAGTCAGGTTCAGATAACTCCAAATCAGAAAACCCTTAGACGGTCTTCAAGGCGACGTTCAGAAG TAGTAGAGTCTACCACTGAAAGCCAAGATAAGGAAAATAGTCATCAAAAAAAGGAACGACGTAAGGAAGA AGAAAAACCTCTTCAGAAGAGTCCATTGCATATAAAAGATGATGTGTTACCTAAACAAAAACTGATTGCT GAACAAACTCTACAGGAGAATTTAATTGAGAAAGGAAGTAATTTACATGAGAAGACTCTTGGGGAAACTA GTGCTAATGCAGAAACTGAACAAAATAAAAAAAAGGCAGACCCTGAGAACATTAAGTCTGAGGGGGATGG TACCCAGGACATTGTAGATAAGTCCTCTGAGAAACTAGTCAGAGGCCGAACACGGTATCAAACTAGAAGA GCATCTCAGGGTTTGCTTTCCAGCATTGAAAACTCAGAATCTGATAGTTCGGAGGCAAAAGAAGAAGGTT CTAGGAAGAAGAGATCTGGAAAATGGAAAAACAAAAGCAATGAAAGTGTTGACATTCAAGATCAAGAAGA GAAAGTGGTGAAACAGGAATGTATAAAAGCTGAAAATCAGTCACATGATTATAAAGCAACTTCTGAAGAA GATGTAAGCATAAAATCTCCGATTTGCGAAAAACAAGATGAAAGTAATACTGTAATATGTCAGGATTCTA CAGTAACTTCAGATTTGTTGCAAGTTCCTGATGATTTACCAAATGTGTGTGAGGAAAAAAATGAAACTAG CAAATATGCAGAATATTCCTTTACAAGTCTACCTGTGCCAGAATCAAATCTAAGGACTAGAAATGCCATT AAGAGATTACATAAGCGAGACTCTTTTGATAATTGTAGTTTGGGAGAATCCTCAAAAATAGGGATATCAG ATATTTCTTCGCTTTCAGAAAAAACTTTTCAAACACTTGAATGCCAACACAAGAGAAGTAGGAGGGTGAG GAGATCTAAAGGTTGTGATTGCTGTGGGGAAAAATCACAACCTCAGGAAAAGTCACTCATTGGGTTAAAG AATACAGAAAATAATGACGTAGAGATTAGTGAAACAAAAAAGGCAGATGTGCAAGCACCTGTAAGCCCAT CAGAAACTTCTCAAGCTAATCCATATTCTGAAGGACAATTTTTAGATGAACATCATAGTGTGAATTTTCA TTTGGGTCTCAAAGAGGATAATGATACTATTAATGATTCATTAATTGTTTCTGAAACCAAATCAAAAGAA AACACTATGCAAGAATCTCTTCCTTCTGGAATAGTAAACTTTAGAGAGGAAATTTGTGATATGGATTCTA GTGAAGCAATGTCTCTTGAAAGCCAGGAGTCACCTAATGAAAATTTTAAAACTGTTGGCCCGTGTTTAGG AGACTCGAAAAATGTTTCACAGGAATCTTTGGAGACAAAAGAAGAAAAACCAGAAGAAACCCCAAAAATG GAACTGAGTCTAGAGAATGTTACTGTTGAAGGAAATGCATGTAAAGTAACAGAATCCAATCTAGAGAAAG CAAAAACTATGGAATTGAATGTAGGAAATGAAGCTAGCTTTCATGGACAAGAGAGAACCAAAACTGGTAT TTCTGAAGAAGCAGCAATAGAAGAAAATAAAAGAAATGATGACTCTGAAGCAGACACAGCTAAACTGAAT GCCAAAGAAGTAGCAACTGAGGAATTTAATTCAGATATTAGTCTTTCTGATAATACTACACCTGTAAAAT TGAATGCTCAAACTGAGATTTCTGAACAAACAGCAGCTGGGGAACTAGATGGAGGAAATGATGTATCTGA TCTACACTCATCTGAAGAAACGAATACCAAAATGAAAAATAATGAAGAAATGATGATCGGCGAGGCAATG GCTGAAACTGGCCATGATGGTGAAACAGAGAATGAGGGCATAACTACCAAAACCTCAAAGCCTGATGAAG CTGAAACAAACATGTTGACTGCAGAAATGGACAACTTTGTTTGTGACACAGTTGAAATGAGCACTGAAGA AGGAATCATTGACGCTAATAAAACTGAAACAAATACTGAGTATAGTAAATCTGAAGAAAAATTAGATAAC AATCAAATGGTAATGGAAAGTGATATTTTACAGGAAGATCACCATACTTCACAGAAAGTGGAGGAACCAT CACAGTGTCTGGCATCTGGAACAGCTATCTCTGAGCTAATAATAGAAGACAATAATGCATCTCCTCAAAA ACTAAGGGAACTTGATCCTTCACTTGTGTCAGCAAATGACAGTCCTAGTGGCATGCAGACACGCTGTGTC TGGTCTCCTTTGGCTTCTCCGTCTACGAGCATTTTAAAGAGAGGACTAAAAAGATCCCAAGAAGATGAAA TCTCATCACCTGTTAATAAGGTTCGCCGTGTCTCCTTTGCAGATCCAATATACCAAGCAGGATTGGCAGA TGACATTGATAGACGGTGCTCTATTGTTAGGTCCCATTCTTCCAATAGTTCTCCCATAGGAAAAAGTGTT AAAACTTCTCCTACTACACAATCTAAGCATAATACCACTTCAGCCAAAGGATTTCTGTCCCCAGGATCAC GTAGCCCTAAATTTAAGAGCTCAAAGAAGTGTTTAATTTCAGAAATGGCCAAAGAATCCATACCATGCCC AACAGAAAGTGTTTACCCACCATTGGTGAACTGTGTGGCACCAGTTGACATCATTTTACCTCAGATTACA TCAAACATGTGGGCAAGAGGCCTGGGACAACTCATTAGAGCTAAGAATATAAAAACTATTGGTGATTTGA GTACTCTTACAGCATCTGAAATAAAAACTCTTCCTATCCGTTCTCCAAAAGTGTCCAATGTAAAAAAGGC TCTCAGAATATATCATGAGCAGCAGGTGAAGACTCGTGGACTAGAAGAGATTCCAGTTTTTGATATTTCT GAAAAAACAGTAAATGGAATAGAAAATAAATCTTTGTCACCTGATGAAGAAAGACTTGTCTCAGATATAA TTGATCCTGTTGCTTTAGAAATTCCATTATCCAAAAACCTTCTGGCACAGATTAGTGCTCTTGCTCTTCA GCTGGATTCAGAAGATCTTCATAATTATTCAGGAAGCCAACTATTTGAAATGCACGAGAAACTAAGTTGT ATGGCAAACTCTGTAATAAAAAATCTACAGTCACGTTGGAGATCACCATCCCATGAAAATTCTATT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223158 representing NM_018151
Red=Cloning site Green=Tags(s) MTARGQSPLAPLLETLEDPSASHGGQTDAYLTLTSRMTGEEGKEVITEIEKKLPRLYKVLKTHISSQNSE LSSAALQALGFCLYNPKITSELSEANALELLSKLNDTIKNSDKNVRTRALWVISKQTFPSEVVGKMVSSI IDSLEILFNKGETHSAVVDFEALNVIVRLIEQAPIQMGEEAVRWAKLVIPLVVHSAQKVHLRGATALEMG MPLLLQKQQEIASITEQLMTTKLISELQKLFMSKNETYVLKLWPLFVKLLGRTLHRSGSFINSLLQLEEL GFRSGAPMIKKIAFIAWKSLIDNFALNPDILCSAKRLKLLMQPLSSIHVRTETLALTKLEVWWYLLMRLG PHLPANFEQVCVPLIQSTISIDSNASPQGNSCHVATSPGLNPMTPVHKGASSPYGAPGTPRMNLSSNLGG MATIPSIQLLGLEMLLHFLLGPEALSFAKQNKLVLSLEPLEHPLISSPSFFSKHANTLITAVHDSFVAVG KDAPDVVVSAIWKELISLVKSVTESGNKKEKPGSEVLTLLLKSLESIVKSEVFPVSKTLVLMEITIKGLP QKVLGSPAYQVANMDILNGTPALFLIQLIFNNFLECGVSDERFFLSLESLVGCVLSGPTSPLAFSDSVLN VINQNAKQLENKEHLWKMWSVIVTPLTELINQTNEVNQGDALEHNFSAIYGALTLPVNHIFSEQRFPVAT MKTLLRTWSELYRAFARCAALVATAEENLCCEELSSKIMSSLEDEGFSNLLFVDRIIYIITVMVDCIDFS PYNIKYQPKVKSPQRPSDWSKKKNEPLGKLTSLFKLIVKVIYSFHTLSFKEAHSDTLFTIGNSITGIISS VLGHISLPSMIRKIFATLTRPLALFYENSKLDEVPKVYSCLNNKLEKLLGEIIACLQFSYTGTYDSELLE QLSPLLCIIFLHKNKQIRKQSAQFWNATFAKVMMLVYPEELKPVLTQAKQKFLLLLPGLETVEMMEESSG PYSDGTENSQLNVKISGMERKSNGKRDSFLAQTKNKKENMKPAAKLKLESSSLKVKGEILLEEEKSTDFV FIPPEGKDAKERILTDHQKEVLKTKRCDIPAMYNNLDVSQDTLFTQYSQEEPMEIPTLTRKPKEDSKMMI TEEQMDSDIVIPQDVTEDCGMAEHLEKSSLSNNECGSLDKTSPEMSNSNNDERKKALISSRKTSTECASS TENSFVVSSSSVSNTTVAGTPPYPTSRRQTFITLEKFDGSENRPFSPSPLNNISSTVTVKNNQETMIKTD FLPKAKQREGTFSKSDSEKIVNGTKRSSRRAGKAEQTGNKRSKPLMRSEPEKNTEESVEGIVVLENNPPG LLNQTECVSDNQVHLSESTMEHDNTKLKAATVENAVLLETNTVEEKNVEINLESKENTPPVVISADQMVN EDSQVQITPNQKTLRRSSRRRSEVVESTTESQDKENSHQKKERRKEEEKPLQKSPLHIKDDVLPKQKLIA EQTLQENLIEKGSNLHEKTLGETSANAETEQNKKKADPENIKSEGDGTQDIVDKSSEKLVRGRTRYQTRR ASQGLLSSIENSESDSSEAKEEGSRKKRSGKWKNKSNESVDIQDQEEKVVKQECIKAENQSHDYKATSEE DVSIKSPICEKQDESNTVICQDSTVTSDLLQVPDDLPNVCEEKNETSKYAEYSFTSLPVPESNLRTRNAI KRLHKRDSFDNCSLGESSKIGISDISSLSEKTFQTLECQHKRSRRVRRSKGCDCCGEKSQPQEKSLIGLK NTENNDVEISETKKADVQAPVSPSETSQANPYSEGQFLDEHHSVNFHLGLKEDNDTINDSLIVSETKSKE NTMQESLPSGIVNFREEICDMDSSEAMSLESQESPNENFKTVGPCLGDSKNVSQESLETKEEKPEETPKM ELSLENVTVEGNACKVTESNLEKAKTMELNVGNEASFHGQERTKTGISEEAAIEENKRNDDSEADTAKLN AKEVATEEFNSDISLSDNTTPVKLNAQTEISEQTAAGELDGGNDVSDLHSSEETNTKMKNNEEMMIGEAM AETGHDGETENEGITTKTSKPDEAETNMLTAEMDNFVCDTVEMSTEEGIIDANKTETNTEYSKSEEKLDN NQMVMESDILQEDHHTSQKVEEPSQCLASGTAISELIIEDNNASPQKLRELDPSLVSANDSPSGMQTRCV WSPLASPSTSILKRGLKRSQEDEISSPVNKVRRVSFADPIYQAGLADDIDRRCSIVRSHSSNSSPIGKSV KTSPTTQSKHNTTSAKGFLSPGSRSPKFKSSKKCLISEMAKESIPCPTESVYPPLVNCVAPVDIILPQIT SNMWARGLGQLIRAKNIKTIGDLSTLTASEIKTLPIRSPKVSNVKKALRIYHEQQVKTRGLEEIPVFDIS EKTVNGIENKSLSPDEERLVSDIIDPVALEIPLSKNLLAQISALALQLDSEDLHNYSGSQLFEMHEKLSC MANSVIKNLQSRWRSPSHENSI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018151 |
| ORF Size | 7416 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018151.5 |
| RefSeq Size | 8383 bp |
| RefSeq ORF | 7419 bp |
| Locus ID | 55183 |
| UniProt ID | Q5UIP0 |
| Gene Summary | This gene encodes a protein that shares homology with the yeast teleomere binding protein, Rap1 interacting factor 1. This protein localizes to aberrant telomeres may be involved in DNA repair. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010] |
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