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UBE2V1 (TMEM189-UBE2V1) (NM_199203) Human Tagged ORF Clone
CAT＃: RG223133
- TrueORF®
TMEM189 (tGFP-tagged) - Human TMEM189-UBE2V1 readthrough (TMEM189-UBE2V1)
ORF Plasmid: DDK
"NM_199203" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CROC-1B; CROC1B; KUA-UEV; TMEM189-UBE2V1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG223133 representing NM_199203
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGCGCCGAGGACTGGCCGGGCCAGCAGCTGGAGCTGGACGAGGACGAGGCGTCTTGTTGCCGCT GGGGCGCGCAGCACGCCGGGGCCCGCGAGCTGGCTGCGCTCTACTCGCCAGGCAAGCGCCTCCAGGAGTG GTGCTCTGTGATCCTGTGCTTCAGCCTCATCGCCCACAACCTGGTCCATCTCCTGCTGCTGGCCCGCTGG GAGGACACACCCCTCGTCATACTCGGTGTTGTTGCAGGGGCTCTCATTGCTGACTTCTTGTCTGGCCTGG TACACTGGGGTGCTGACACATGGGGCTCTGTGGAGCTGCCCATTGTGGGGAAGGCTTTCATCCGACCCTT CCGGGAGCACCACATTGACCCGACAGCTATCACACGGCACGACTTCATCGAGACCAACGGGGACAACTGC CTGGTGACACTGCTGCCGCTGCTAAACATGGCCTACAAGTTCCGCACCCACAGCCCTGAAGCCCTGGAGC AGCTATACCCCTGGGAGTGCTTCGTCTTCTGCCTGATCATCTTCGGCACCTTCACCAACCAGATCCACAA GTGGTCGCACACGTACTTTGGGCTGCCACGCTGGGTCACCCTCCTGCAGGACTGGCATGTCATCCTGCCA CGTAAACACCATCGCATCCACCACGTCTCACCCCACGAGACCTACTTCTGCATCACCACAGGAGTAAAAG TCCCTCGCAATTTCCGACTGTTGGAAGAACTCGAAGAAGGCCAGAAAGGAGTAGGAGATGGCACAGTTAG CTGGGGTCTAGAAGATGACGAAGACATGACACTTACAAGATGGACAGGGATGATAATTGGGCCTCCAAGA ACAATTTATGAAAACCGAATATACAGCCTTAAAATAGAATGTGGACCTAAATACCCAGAAGCACCCCCCT TTGTAAGATTTGTAACAAAAATTAATATGAATGGAGTAAATAGTTCTAATGGAGTGGTGGACCCAAGAGC CATATCAGTGCTAGCAAAATGGCAGAATTCATATAGCATCAAAGTTGTCCTGCAAGAGCTTCGGCGCCTA ATGATGTCTAAAGAAAATATGAAACTCCCTCAGCCGCCCGAAGGACAGTGTTACAGCAAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223133 representing NM_199203
Red=Cloning site Green=Tags(s) MAGAEDWPGQQLELDEDEASCCRWGAQHAGARELAALYSPGKRLQEWCSVILCFSLIAHNLVHLLLLARW EDTPLVILGVVAGALIADFLSGLVHWGADTWGSVELPIVGKAFIRPFREHHIDPTAITRHDFIETNGDNC LVTLLPLLNMAYKFRTHSPEALEQLYPWECFVFCLIIFGTFTNQIHKWSHTYFGLPRWVTLLQDWHVILP RKHHRIHHVSPHETYFCITTGVKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPPR TIYENRIYSLKIECGPKYPEAPPFVRFVTKINMNGVNSSNGVVDPRAISVLAKWQNSYSIKVVLQELRRL MMSKENMKLPQPPEGQCYSN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_199203 |
| ORF Size | 1110 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_199203.2, NP_954673.1 |
| RefSeq Size | 2852 bp |
| RefSeq ORF | 1113 bp |
| Locus ID | 387522 |
| UniProt ID | Q13404 |
| Protein Families | Transmembrane |
| Gene Summary | The TMEM189-UEV mRNA is an infrequent but naturally occurring read-through transcript of the neighboring TMEM189 and UBE2V1 genes. Ubiquitin-conjugating E2 enzyme variant proteins constitute a distinct subfamily within the E2 protein family. They have sequence similarity to other ubiquitin-conjugating enzymes but lack the conserved cysteine residue that is critical for the catalytic activity of E2s. The protein produced by this transcript has UEV1 B domains but the protein is localized to the cytoplasm rather than to the nucleus. The significance of this read-through mRNA and the function of its protein product has not yet been determined. [provided by RefSeq, Oct 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223133 | TMEM189 (Myc-DDK-tagged)-Human TMEM189-UBE2V1 readthrough (TMEM189-UBE2V1) |
CNY 3,656.00 |
|
| RC223133L3 | Lenti ORF clone of Human TMEM189-UBE2V1 readthrough (TMEM189-UBE2V1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC223133L4 | Lenti ORF clone of Human TMEM189-UBE2V1 readthrough (TMEM189-UBE2V1), mGFP tagged |
CNY 5,890.00 |
|
| SC313174 | TMEM189 (untagged)-Human TMEM189-UBE2V1 readthrough (TMEM189-UBE2V1) |
CNY 6,080.00 |
