United States
Germany
Japan
United Kingdom
China
DMGDH (NM_013391) Human Tagged ORF Clone
CAT＃: RG223089
- TrueORF®
DMGDH (tGFP-tagged) - Human dimethylglycine dehydrogenase (DMGDH), nuclear gene encoding mitochondrial protein
ORF Plasmid: DDK
"NM_013391" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DMGDHD; ME2GLYDH |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG223089 representing NM_013391
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCCGTCCCGGCGCGCAGCTGCTGCGGGGCCTCCTGCTGCGGAGCTGCCCGCTGCAGGGCTCCCCCG GGCGCCCGCGCTCTGTCTGCGGCCGGGAAGGAGAGGAAAAACCACCCTTATCTGCAGAAACACAATGGAA AGACAGAGCAGAAACAGTGATAATTGGAGGTGGCTGTGTTGGTGTGAGTCTGGCTTATCACCTGGCCAAA GCAGGGATGAAAGATGTGGTCCTGCTGGAGAAATCAGAGCTCACGGCTGGATCTACCTGGCACGCAGCAG GTTTAACAACTTACTTTCATCCTGGAATAAACTTGAAGAAAATACATTATGATAGCATCAAACTTTATGA GAAACTGGAAGAAGAAACTGGTCAGGTGGTGGGATTCCATCAGCCAGGTAGTATCAGACTTGCTACCACC CCTGTAAGGGTAGATGAATTTAAATATCAAATGACTCGGACTGGCTGGCATGCAACAGAACAGTATCTCA TTGAACCTGAAAAAATTCAAGAGATGTTCCCTTTACTCAACATGAATAAGGTTTTAGCTGGATTGTATAA TCCTGGAGATGGTCACATTGATCCTTATTCTCTAACTATGGCACTGGCTGCTGGGGCTAGGAAATGTGGT GCCCTTTTAAAATATCCTGCACCAGTAACTTCTCTGAAAGCCAGGTCAGATGGAACATGGGACGTTGAAA CACCACAGGGGTCTATGAGAGCAAATAGAATTGTGAATGCTGCAGGATTTTGGGCTCGTGAAGTAGGTAA AATGATTGGACTAGAACATCCTCTCATTCCGGTTCAACATCAATATGTTGTTACATCGACTATATCTGAA GTGAAAGCTTTGAAACGAGAACTGCCTGTGCTCCGTGACCTGGAAGGATCATATTATCTCCGACAGGAAA GGGATGGGCTTTTGTTTGGTCCATATGAAAGTCAAGAGAAAATGAAAGTTCAGGACTCCTGGGTCACCAA TGGAGTTCCTCCAGGTTTTGGAAAGGAACTCTTTGAGTCTGATCTAGATCGAATCATGGAACACATCAAA GCTGCCATGGAAATGGTTCCTGTCTTGAAAAAGGCTGACATCATCAATGTTGTCAATGGTCCTATCACGT ATTCTCCTGACATTCTGCCTATGGTGGGGCCCCATCAGGGGGTCAGAAACTACTGGGTGGCTATAGGCTT TGGATATGGCATAATCCACGCTGGTGGGGTAGGGAAATATCTCAGTGACTGGATCCTGCATGGAGAACCT CCTTTTGATCTGATAGAATTGGATCCTAATCGCTATGGCAAATGGACAACAACCCAGTACACTGAGGCCA AAGCAAGAGAATCATATGGATTCAACAATATTGTTGGTTATCCTAAAGAAGAACGGTTTGCTGGGAGGCC GACTCAACGAGTCAGTGGGCTCTATCAAAGGCTGGAGTCTAAGTGTTCCATGGGGTTCCATGCTGGCTGG GAGCAGCCGCACTGGTTCTACAAACCAGGCCAGGACACTCAGTACAGGCCAAGTTTTCGCCGCACAAACT GGTTTGAGCCTGTGGGCTCGGAGTATAAACAGGTTATGCAAAGAGTAGCGGTAACTGACCTATCACCATT TGGCAAGTTTAACATCAAAGGCCAAGATTCCATTAGACTACTGGACCATCTCTTTGCAAATGTCATTCCA AAGGTGGGTTTTACAAATATAAGTCACATGTTAACACCCAAGGGTCGAGTGTATGCTGAGCTGACTGTTT CTCACCAATCTCCTGGGGAGTTTCTTTTAATTACTGGCTCTGGATCAGAACTTCATGATCTTAGATGGAT TGAAGAAGAAGCAGTCAAAGGTGGATATGATGTTGAAATTAAAAACATAACTGATGAGCTTGGAGTTCTT GGAGTTGCTGGGCCACAGGCAAGAAAGGTCCTTCAGAAACTGACCTCTGAAGATCTTAGTGATGATGTTT TCAAGTTTCTTCAAACCAAGTCCTTAAAGGTTTCCAACATTCCTGTCACTGCTATTAGGATATCTTATAC TGGTGAGCTGGGTTGGGAGCTGTATCACAGAAGAGAAGATTCTGTGGCGCTGTATGACGCTATCATGAAT GCAGGCCAGGAGGAGGGAATCGACAATTTTGGAACCTATGCCATGAATGCCTTACGCCTGGAGAAAGCCT TCAGAGCCTGGGGGTTAGAGATGAACTGTGATACAAATCCTTTGGAAGCTGGACTGGAATATTTTGTGAA GTTAAATAAGCCAGCAGACTTCATAGGAAAGCAAGCACTGAAACAGATTAAAGCCAAGGGGCTGAAACGA AGACTGGTCTGCCTCACCTTGGCAACGGATGATGTTGATCCAGAGGGAAATGAAAGCATCTGGTACAATG GCAAGGTGGTTGGCAACACGACATCTGGAAGCTATAGCTACAGCATCCAGAAGAGTCTGGCTTTCGCATA TGTCCCTGTACAACTAAGTGAAGTGGGACAGCAAGTGGAAGTTGAACTATTAGGCAAAAATTACCCAGCA GTCATCATACAAGAACCTTTGGTATTGACCGAACCAACCAGAAACCGGCTTCAGAAAAAAGGTGGAAAGG ACAAAACT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223089 representing NM_013391
Red=Cloning site Green=Tags(s) MLRPGAQLLRGLLLRSCPLQGSPGRPRSVCGREGEEKPPLSAETQWKDRAETVIIGGGCVGVSLAYHLAK AGMKDVVLLEKSELTAGSTWHAAGLTTYFHPGINLKKIHYDSIKLYEKLEEETGQVVGFHQPGSIRLATT PVRVDEFKYQMTRTGWHATEQYLIEPEKIQEMFPLLNMNKVLAGLYNPGDGHIDPYSLTMALAAGARKCG ALLKYPAPVTSLKARSDGTWDVETPQGSMRANRIVNAAGFWAREVGKMIGLEHPLIPVQHQYVVTSTISE VKALKRELPVLRDLEGSYYLRQERDGLLFGPYESQEKMKVQDSWVTNGVPPGFGKELFESDLDRIMEHIK AAMEMVPVLKKADIINVVNGPITYSPDILPMVGPHQGVRNYWVAIGFGYGIIHAGGVGKYLSDWILHGEP PFDLIELDPNRYGKWTTTQYTEAKARESYGFNNIVGYPKEERFAGRPTQRVSGLYQRLESKCSMGFHAGW EQPHWFYKPGQDTQYRPSFRRTNWFEPVGSEYKQVMQRVAVTDLSPFGKFNIKGQDSIRLLDHLFANVIP KVGFTNISHMLTPKGRVYAELTVSHQSPGEFLLITGSGSELHDLRWIEEEAVKGGYDVEIKNITDELGVL GVAGPQARKVLQKLTSEDLSDDVFKFLQTKSLKVSNIPVTAIRISYTGELGWELYHRREDSVALYDAIMN AGQEEGIDNFGTYAMNALRLEKAFRAWGLEMNCDTNPLEAGLEYFVKLNKPADFIGKQALKQIKAKGLKR RLVCLTLATDDVDPEGNESIWYNGKVVGNTTSGSYSYSIQKSLAFAYVPVQLSEVGQQVEVELLGKNYPA VIIQEPLVLTEPTRNRLQKKGGKDKT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013391 |
| ORF Size | 2598 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013391.3 |
| RefSeq Size | 3104 bp |
| RefSeq ORF | 2601 bp |
| Locus ID | 29958 |
| UniProt ID | Q9UI17 |
| Domains | DAO, GCV_T |
| Protein Pathways | Glycine, serine and threonine metabolism, Metabolic pathways |
| Gene Summary | This gene encodes an enzyme involved in the catabolism of choline, catalyzing the oxidative demethylation of dimethylglycine to form sarcosine. The enzyme is found as a monomer in the mitochondrial matrix, and uses flavin adenine dinucleotide and folate as cofactors. Mutation in this gene causes dimethylglycine dehydrogenase deficiency, characterized by a fishlike body odor, chronic muscle fatigue, and elevated levels of the muscle form of creatine kinase in serum. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223089 | DMGDH (Myc-DDK-tagged)-Human dimethylglycine dehydrogenase (DMGDH), nuclear gene encoding mitochondrial protein |
CNY 6,344.00 |
|
| RC223089L3 | Lenti-ORF clone of DMGDH (Myc-DDK-tagged)-Human dimethylglycine dehydrogenase (DMGDH), nuclear gene encoding mitochondrial protein |
CNY 9,030.00 |
|
| RC223089L4 | Lenti-ORF clone of DMGDH (mGFP-tagged)-Human dimethylglycine dehydrogenase (DMGDH), nuclear gene encoding mitochondrial protein |
CNY 9,030.00 |
|
| SC124128 | DMGDH (untagged)-Human dimethylglycine dehydrogenase (DMGDH), nuclear gene encoding mitochondrial protein |
CNY 16,720.00 |
