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EDC4 (NM_014329) Human Tagged ORF Clone
CAT＃: RG222770
- TrueORF®
EDC4 (tGFP-tagged) - Human enhancer of mRNA decapping 4 (EDC4)
ORF Plasmid: DDK
"NM_014329" in other vectors (6)
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CNY 6,270.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Ge-1; GE1; HEDL5; HEDLS; RCD-8; RCD8 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG222770 representing NM_014329
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCCTGCGCGAGCATCGACATCGAGGACGCCACGCAGCACCTGCGGGACATCCTCAAGCTGGACC GGCCCGCGGGCGGCCCCAGTGCAGAGAGCCCACGGCCATCCAGTGCCTACAATGGGGACCTCAATGGACT TCTGGTCCCAGACCCGCTCTGCTCAGGTGATAGTACCTCAGCAAACAAGACTGGTCTTCGGACCATGCCA CCCATTAACCTGCAAGAGAAGCAGGTCATCTGTCTCTCAGGAGATGATAGCTCCACCTGCATTGGGATTT TGGCCAAGGAGGTGGAGATTGTGGCTAGCAGTGACTCTAGCATTTCAAGCAAGGCCCGGGGAAGCAACAA GGTGAAAATTCAGCCTGTCGCCAAGTATGACTGGGAACAGAAGTACTACTATGGCAACCTGATTGCTGTG TCTAACTCCTTCTTGGCCTATGCCATTCGGGCTGCCAACAATGGCTCTGCCATGGTGCGGGTGATCAGCG TCAGCACTTCGGAGCGGACCTTGCTCAAGGGCTTCACAGGCAGTGTGGCTGATCTGGCTTTCGCGCACCT CAACTCTCCACAGCTGGCCTGCCTGGATGAGGCAGGCAACCTGTTCGTGTGGCGCTTGGCTCTGGTTAAT GGCAAAATTCAAGAAGAGATCTTGGTCCATATTCGGCAGCCAGAGGGCACGCCACTGAACCACTTTCGCA GGATCATCTGGTGCCCCTTCATCCCTGAGGAGAGCGAAGACTGCTGTGAGGAGAGCAGCCCAACAGTGGC CCTGCTGCATGAAGACCGGGCTGAGGTGTGGGACCTGGACATGCTCCGCTCCAGCCACAGTACCTGGCCT GTGGATGTTAGCCAGATCAAGCAGGGCTTCATTGTGGTAAAAGGTCATAGCACGTGCCTCAGTGAAGGAG CCCTCTCTCCTGATGGGACTGTGCTGGCTACTGCGAGCCACGATGGCTATGTCAAGTTCTGGCAGATCTA CATTGAGGGGCAAGATGAGCCAAGGTGTCTGCACGAGTGGAAACCTCATGATGGGCGGCCCCTCTCCTGC CTCCTGTTCTGTGACAACCATAAGAAACAAGACCCTGATGTCCCTTTCTGGAGGTTCCTTATTACTGGTG CTGACCAGAACCGAGAGTTAAAGATGTGGTGTACAGTATCCTGGACCTGCCTGCAGACTATTCGCTTCTC CCCAGATATCTTCAGCTCAGTGAGTGTGCCCCCTAGCCTCAAGGTTTGCTTGGACCTCTCAGCAGAATAC CTGATTCTCAGCGATGTGCAACGGAAGGTCCTCTATGTGATGGAGCTGCTGCAAAACCAGGAGGAGGGCC ACGCCTGCTTCAGCTCCATCTCGGAGTTCCTGCTCACCCACCCTGTGCTGAGCTTTGGTATCCAGGTTGT GAGTCGCTGCCGGCTACGGCACACTGAGGTGCTGCCTGCCGAAGAGGAAAATGACAGCCTGGGTGCTGAT GGTACCCATGGAGCCGGTGCCATGGAGTCTGCGGCCGGTGTGCTCATCAAGCTCTTTTGTGTGCATACTA AGGCACTGCAAGATGTGCAGATCCGCTTCCAGCCACAGCTGAACCCTGATGTGGTGGCCCCACTGCCCAC CCACACTGCCCACGAGGACTTCACATTTGGAGAGTCTCGGCCCGAACTGGGCTCTGAGGGCCTGGGGTCA GCCGCTCACGGCTCCCAGCCTGACCTCCGACGAATCGTGGAGCTGCCTGCACCTGCCGACTTCCTCAGTC TGAGCAGTGAGACCAAGCCCAAGTTGATGACACCTGACGCCTTCATGACACCTAGCGCCTCCTTGCAGCA GATCACTGCCTCTCCCAGCAGCAGCAGCAGCGGTAGCAGCAGCAGCAGCAGCAGTAGCAGCAGCTCCCTT ACAGCTGTGTCTGCCATGAGCAGCACCTCAGCTGTGGACCCCTCCTTGACCAGGCCACCTGAGGAGCTGA CCTTGAGCCCCAAGCTGCAGCTGGATGGCAGCCTGACAATGAGCAGCAGTGGCAGCCTTCAGGCAAGCCC GCGTGGCCTCCTGCCTGGCCTGCTCCCAGCCCCAGCTGACAAACTGACTCCCAAGGGGCCGGGCCAGGTG CCTACTGCCACCTCTGCACTGTCCCTGGAGCTGCAGGAAGTGGAGCCCCTGGGGCTACCCCAAGCCTCCC CTAGCCGCACTCGTTCCCCTGATGTCATCTCCTCAGCTTCCACTGCCCTGTCCCAGGACATCCCTGAGAT TGCATCTGAGGCCCTGTCCCGTGGTTTTGGCTCCTCTGCACCAGAGGGCCTTGAGCCAGACAGTATGGCT TCAGCCGCCTCGGCACTGCACCTGCTGTCCCCACGGCCCCGGCCAGGGCCCGAGCTCGGCCCCCAGCTCG GGCTTGATGGAGGCCCTGGGGATGGAGATCGGCATAATACCCCCTCCCTCCTGGAGGCAGCCTTGACCCA GGAGGCCTCGACTCCTGACAGTCAGGTTTGGCCCACAGCACCTGACATTACTCGTGAGACCTGCAGCACC CTGGCAGAAAGCCCCAGGAATGGCCTTCAGGAAAAGCACAAGAGCCTGGCCTTCCACCGACCACCATATC ACCTGCTGCAGCAACGTGACAGCCAGGATGCCAGTGCTGAGCAAAGTGACCATGATGATGAGGTGGCCAG CCTTGCCTCTGCTTCAGGAGGCTTTGGCACCAAAGTTCCTGCTCCACGGCTGCCTGCCAAGGACTGGAAG ACCAAGGGATCCCCTCGAACCTCACCCAAGCTCAAGAGGAAAAGCAAGAAGGATGATGGGGATGCAGCCA TGGGATCCCGGCTCACAGAGCACCAGGTGGCAGAGCCCCCTGAGGACTGGCCAGCACTAATTTGGCAACA GCAGAGAGAGCTGGCAGAGCTGCGGCACAGCCAGGAAGAGCTGCTGCAGCGTCTGTGTACCCAACTCGAA GGCCTGCAGAGCACAGTCACAGGCCACGTAGAACGTGCCCTTGAGACTCGGCACGAGCAGGAACAGCGGC GGCTGGAGCGAGCACTGGCTGAGGGGCAGCAGCGGGGAGGGCAGCTGCAGGAGCAGCTGACACAACAGTT GTCCCAAGCACTGTCGTCAGCTGTAGCTGGGCGGCTAGAGCGCAGCATACGGGATGAGATCAAGAAGACA GTCCCTCCATGTGTCTCAAGGAGTCTGGAGCCTATGGCAGGCCAACTGAGCAACTCAGTGGCTACCAAGC TCACAGCTGTGGAGGGCAGCATGAAAGAGAACATCTCCAAGCTGCTCAAGTCCAAGAACTTGACTGATGC CATCGCCCGAGCAGCTGCAGACACATTACAAGGGCCGATGCAGGCTGCCTACCGGGAAGCCTTCCAGAGT GTGGTGCTGCCGGCCTTTGAGAAGAGCTGCCAGGCCATGTTCCAGCAAATCAATGATAGCTTCCGGCTGG GGACACAGGAATACTTGCAGCAGCTAGAAAGCCACATGAAGAGCCGGAAGGCACGGGAACAGGAGGCCAG GGAGCCTGTGCTAGCCCAGCTGCGGGGCCTGGTCAGCACACTGCAGAGTGCCACTGAGCAGATGGCAGCC ACCGTGGCCGGCAGTGTTCGTGCTGAGGTGCAGCACCAGCTGCATGTGGCTGTGGGCAGCCTGCAGGAGT CCATTTTAGCACAGGTACAGCGCATCGTTAAGGGTGAGGTGAGTGTGGCGCTCAAGGAGCAGCAGGCCGC CGTCACCTCCAGCATCATGCAGGCCATGCGCTCAGCTGCTGGCACACCTGTCCCCTCTGCCCACCTTGAC TGCCAGGCCCAGCAAGCCCATATCCTGCAGCTGCTGCAGCAGGGCCACCTCAATCAGGCCTTCCAGCAGG CGCTGACAGCTGCTGACCTGAACCTGGTGCTGTATGTGTGTGAAACTGTGGACCCAGCCCAGGTTTTTGG GCAGCCACCCTGCCCGCTCTCCCAGCCTGTGCTCCTTTCCCTCATCCAGCAGCTGGCATCTGACCTTGGC ACTCGAACTGACCTCAAGCTCAGCTACCTGGAAGAGGCCGTGATGCACCTGGACCACAGTGACCCCATCA CTCGGGACCACATGGGCTCCGTTATGGCCCAGGTGCGCCAAAAGCTTTTTCAGTTCCTGCAGGCTGAGCC ACACAACTCACTTGGCAAAGCAGCTCGGCGTCTCAGCCTCATGCTGCATGGCCTCGTGACCCCCAGCCTC CCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222770 representing NM_014329
Red=Cloning site Green=Tags(s) MASCASIDIEDATQHLRDILKLDRPAGGPSAESPRPSSAYNGDLNGLLVPDPLCSGDSTSANKTGLRTMP PINLQEKQVICLSGDDSSTCIGILAKEVEIVASSDSSISSKARGSNKVKIQPVAKYDWEQKYYYGNLIAV SNSFLAYAIRAANNGSAMVRVISVSTSERTLLKGFTGSVADLAFAHLNSPQLACLDEAGNLFVWRLALVN GKIQEEILVHIRQPEGTPLNHFRRIIWCPFIPEESEDCCEESSPTVALLHEDRAEVWDLDMLRSSHSTWP VDVSQIKQGFIVVKGHSTCLSEGALSPDGTVLATASHDGYVKFWQIYIEGQDEPRCLHEWKPHDGRPLSC LLFCDNHKKQDPDVPFWRFLITGADQNRELKMWCTVSWTCLQTIRFSPDIFSSVSVPPSLKVCLDLSAEY LILSDVQRKVLYVMELLQNQEEGHACFSSISEFLLTHPVLSFGIQVVSRCRLRHTEVLPAEEENDSLGAD GTHGAGAMESAAGVLIKLFCVHTKALQDVQIRFQPQLNPDVVAPLPTHTAHEDFTFGESRPELGSEGLGS AAHGSQPDLRRIVELPAPADFLSLSSETKPKLMTPDAFMTPSASLQQITASPSSSSSGSSSSSSSSSSSL TAVSAMSSTSAVDPSLTRPPEELTLSPKLQLDGSLTMSSSGSLQASPRGLLPGLLPAPADKLTPKGPGQV PTATSALSLELQEVEPLGLPQASPSRTRSPDVISSASTALSQDIPEIASEALSRGFGSSAPEGLEPDSMA SAASALHLLSPRPRPGPELGPQLGLDGGPGDGDRHNTPSLLEAALTQEASTPDSQVWPTAPDITRETCST LAESPRNGLQEKHKSLAFHRPPYHLLQQRDSQDASAEQSDHDDEVASLASASGGFGTKVPAPRLPAKDWK TKGSPRTSPKLKRKSKKDDGDAAMGSRLTEHQVAEPPEDWPALIWQQQRELAELRHSQEELLQRLCTQLE GLQSTVTGHVERALETRHEQEQRRLERALAEGQQRGGQLQEQLTQQLSQALSSAVAGRLERSIRDEIKKT VPPCVSRSLEPMAGQLSNSVATKLTAVEGSMKENISKLLKSKNLTDAIARAAADTLQGPMQAAYREAFQS VVLPAFEKSCQAMFQQINDSFRLGTQEYLQQLESHMKSRKAREQEAREPVLAQLRGLVSTLQSATEQMAA TVAGSVRAEVQHQLHVAVGSLQESILAQVQRIVKGEVSVALKEQQAAVTSSIMQAMRSAAGTPVPSAHLD CQAQQAHILQLLQQGHLNQAFQQALTAADLNLVLYVCETVDPAQVFGQPPCPLSQPVLLSLIQQLASDLG TRTDLKLSYLEEAVMHLDHSDPITRDHMGSVMAQVRQKLFQFLQAEPHNSLGKAARRLSLMLHGLVTPSL P TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014329 |
| ORF Size | 4203 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014329.5 |
| RefSeq Size | 4811 bp |
| RefSeq ORF | 4206 bp |
| Locus ID | 23644 |
| UniProt ID | Q6P2E9 |
| Domains | WD40 |
| Protein Pathways | RNA degradation |
| Gene Summary | In the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222770 | EDC4 (Myc-DDK-tagged)-Human enhancer of mRNA decapping 4 (EDC4) |
CNY 14,376.00 |
|
| RC222770L1 | Lenti-ORF clone of EDC4 (Myc-DDK-tagged)-Human enhancer of mRNA decapping 4 (EDC4) |
CNY 16,776.00 |
|
| RC222770L2 | Lenti-ORF clone of EDC4 (mGFP-tagged)-Human enhancer of mRNA decapping 4 (EDC4) |
CNY 7,600.00 |
|
| RC222770L3 | Lenti-ORF clone of EDC4 (Myc-DDK-tagged)-Human enhancer of mRNA decapping 4 (EDC4) |
CNY 7,600.00 |
|
| RC222770L4 | Lenti-ORF clone of EDC4 (mGFP-tagged)-Human enhancer of mRNA decapping 4 (EDC4) |
CNY 7,600.00 |
|
| SC308921 | EDC4 (untagged)-Human enhancer of mRNA decapping 4 (EDC4) |
CNY 10,450.00 |
