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SF3B2 (NM_006842) Human Tagged ORF Clone
CAT＃: RG222762
- TrueORF®
SF3B2 (tGFP-tagged) - Human splicing factor 3b, subunit 2, 145kDa (SF3B2)
ORF Plasmid: DDK
"NM_006842" in other vectors (5)
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CNY 7,890.00
Product images
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Cus1; SAP145; SF3b1; SF3B145; SF3b150 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG222762 representing NM_006842
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACGGAGCATCCCGAGCCTCCCAAAGCAGAATTGCAGCTGCCGCCGCCGCCACCTCCAGGCCACT ATGGCGCCTGGGCTGCCCAGGAGCTTCAGGCCAAGTTGGCAGAGATCGGAGCTCCGATCCAGGGTAATCG CGAGGAGCTGGTGGAGCGGCTGCAGAGCTACACCCGCCAGACTGGCATCGTGCTGAATCGGCCGGTTTTG AGAGGGGAAGATGGGGACAAAGCCGCTCCACCTCCCATGTCGGCACAGCTCCCTGGAATTCCCATGCCAC CACCACCTTTGGGACTCCCCCCTCTGCAGCCTCCTCCGCCACCCCCACCACCTCCACCAGGCCTTGGCCT TGGCTTTCCTATGGCCCACCCACCAAATTTGGGGCCCCCGCCTCCTCTCCGTGTGGGTGAGCCAGTGGCA CTGTCAGAGGAGGAGCGGCTGAAGTTGGCTCAGCAGCAGGCGGCATTGCTGATGCAGCAGGAGGAGCGTG CCAAGCAGCAGGGAGATCATTCGCTGAAGGAACATGAGCTCTTGGAGCAGCAGAAGCGGGCAGCTGTGTT ACTGGAGCAGGAACGACAGCAGGAGATTGCCAAGATGGGCACCCCAGTCCCTCGGCCCCCACAAGACATG GGCCAGATTGGTGTGCGCACTCCTCTGGGTCCTCGAGTAGCTGCTCCAGTGGGCCCAGTGGGCCCCACTC CTACAGTTTTGCCCATGGGAGCCCCTGTTCCCCGGCCTCGTGGTCCCCCACCGCCCCCTGGAGATGAGAA CAGAGAGATGGATGACCCCTCTGTGGGCCCCAAGATCCCCCAGGCTTTGGAGAAGATCCTGCAGCTGAAG GAGAGCCGCCAGGAAGAGATGAATTCTCAGCAGGAGGAAGAGGAAATGGAAACAGATGCTCGCTCGTCCC TGGGCCAGTCAGCGTCAGAGACTGAGGAGGACACAGTGTCCGTATCTAAAAAGGAGAAAAACCGGAAGCG TAGGAACCGAAAGAAGAAGAAAAAGCCCCAGCGGGTGCGAGGGGTGTCCTCTGAGAGCTCTGGGGACCGG GAGAAAGACTCAACCCGGTCCCGTGGCTCTGATTCCCCAGCAGCTGATGTTGAGATTGAGTATGTGACTG AAGAACCTGAAATTTACGAGCCCAACTTTATCTTCTTTAAGAGGATCTTTGAGGCTTTTAAGCTCACTGA TGATGTGAAGAAGGAGAAAGAGAAGGAGCCAGAGAAACTTGACAAACTGGAGAACTCTGCAGCCCCCAAG AAGAAGGGATTTGAAGAGGAGCACAAGGACAGTGATGATGACAGCAGTGATGACGAGCAGGAAAAGAAGC CAGAAGCCCCCAAGCTGTCCAAGAAGAAGTTGCGCCGAATGAACCGCTTCACTGTGGCTGAACTCAAGCA GCTGGTGGCTCGGCCCGATGTCGTGGAGATGCACGATGTGACAGCGCAGGACCCTAAGCTCTTGGTTCAC CTCAAGGCCACTCGGAACTCTGTGCCTGTGCCACGCCACTGGTGTTTTAAGCGCAAATACCTGCAGGGCA AACGGGGCATTGAGAAGCCCCCCTTCGAGCTGCCAGACTTCATCAAACGCACAGGCATCCAGGAGATGCG AGAGGCCCTGCAGGAGAAGGAAGAACAGAAGACCATGAAGTCAAAAATGCGAGAGAAAGTTCGGCCTAAG ATGGGCAAAATTGACATCGACTACCAGAAACTGCATGATGCCTTCTTCAAGTGGCAGACCAAGCCAAAGC TGACCATCCATGGGGACCTGTACTATGAGGGGAAGGAGTTCGAGACACGACTGAAGGAGAAGAAGCCAGG AGATCTGTCTGATGAGCTAAGGATTTCCTTGGGGATGCCAGTAGGACCAAATGCCCACAAGGTCCCTCCC CCATGGCTGATTGCCATGCAGCGATATGGACCACCCCCATCGTATCCCAACCTGAAAATCCCTGGGCTGA ACTCGCCCATCCCTGAGAGCTGTTCCTTTGGGTACCATGCTGGTGGCTGGGGCAAACCTCCAGTGGATGA GACTGGGAAACCGCTCTATGGGGACGTGTTTGGAACCAATGCTGCTGAATTTCAGACCAAGACTGAGGAA GAAGAGATTGATCGGACCCCTTGGGGGGAACTGGAACCATCTGATGAAGAATCCTCAGAAGAAGAGGAAG AGGAAGAAAGTGATGAAGACAAACCAGATGAGACAGGCTTTATTACCCCTGCAGACAGTGGCCTTATCAC TCCTGGAGGCTTTTCATCAGTGCCTGCTGGAATGGAGACCCCTGAACTCATTGAGCTGAGGAAGAAGAAG ATTGAGGAGGCGATGGACGGAAGTGAGACACCTCAGCTCTTCACTGTGTTGCCAGAGAAGAGAACAGCCA CTGTTGGAGGGGCCATGATGGGATCAACCCACATTTATGACATGTCCACGGTTATGAGCCGGAAGGGCCC GGCTCCTGAGCTGCAAGGTGTGGAAGTGGCGCTGGCGCCTGAAGAGTTGGAGCTGGATCCTATGGCCATG ACCCAGAAGTATGAGGAGCATGTGCGGGAGCAGCAGGCTCAAGTAGAGAAGGAGGACTTCAGTGACATGG TGGCTGAGCACGCTGCCAAACAGAAGCAAAAAAAACGGAAAGCTCAGCCCCAGGACAGCCGTGGGGGCAG CAAGAAATATAAGGAGTTCAAGTTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222762 representing NM_006842
Red=Cloning site Green=Tags(s) MATEHPEPPKAELQLPPPPPPGHYGAWAAQELQAKLAEIGAPIQGNREELVERLQSYTRQTGIVLNRPVL RGEDGDKAAPPPMSAQLPGIPMPPPPLGLPPLQPPPPPPPPPPGLGLGFPMAHPPNLGPPPPLRVGEPVA LSEEERLKLAQQQAALLMQQEERAKQQGDHSLKEHELLEQQKRAAVLLEQERQQEIAKMGTPVPRPPQDM GQIGVRTPLGPRVAAPVGPVGPTPTVLPMGAPVPRPRGPPPPPGDENREMDDPSVGPKIPQALEKILQLK ESRQEEMNSQQEEEEMETDARSSLGQSASETEEDTVSVSKKEKNRKRRNRKKKKKPQRVRGVSSESSGDR EKDSTRSRGSDSPAADVEIEYVTEEPEIYEPNFIFFKRIFEAFKLTDDVKKEKEKEPEKLDKLENSAAPK KKGFEEEHKDSDDDSSDDEQEKKPEAPKLSKKKLRRMNRFTVAELKQLVARPDVVEMHDVTAQDPKLLVH LKATRNSVPVPRHWCFKRKYLQGKRGIEKPPFELPDFIKRTGIQEMREALQEKEEQKTMKSKMREKVRPK MGKIDIDYQKLHDAFFKWQTKPKLTIHGDLYYEGKEFETRLKEKKPGDLSDELRISLGMPVGPNAHKVPP PWLIAMQRYGPPPSYPNLKIPGLNSPIPESCSFGYHAGGWGKPPVDETGKPLYGDVFGTNAAEFQTKTEE EEIDRTPWGELEPSDEESSEEEEEEESDEDKPDETGFITPADSGLITPGGFSSVPAGMETPELIELRKKK IEEAMDGSETPQLFTVLPEKRTATVGGAMMGSTHIYDMSTVMSRKGPAPELQGVEVALAPEELELDPMAM TQKYEEHVREQQAQVEKEDFSDMVAEHAAKQKQKKRKAQPQDSRGGSKKYKEFKF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006842 |
| ORF Size | 2685 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006842.3 |
| RefSeq Size | 2902 bp |
| RefSeq ORF | 2688 bp |
| Locus ID | 10992 |
| UniProt ID | Q13435 |
| Domains | SAP, PSP, DUF382 |
| Protein Pathways | Spliceosome |
| Gene Summary | This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222762 | SF3B2 (Myc-DDK-tagged)-Human splicing factor 3b, subunit 2, 145kDa (SF3B2) |
CNY 6,552.00 |
|
| RC222762L3 | Lenti-ORF clone of SF3B2 (Myc-DDK-tagged)-Human splicing factor 3b, subunit 2, 145kDa (SF3B2) |
CNY 9,030.00 |
|
| RC222762L4 | Lenti-ORF clone of SF3B2 (mGFP-tagged)-Human splicing factor 3b, subunit 2, 145kDa (SF3B2) |
CNY 9,030.00 |
|
| SC115836 | SF3B2 (untagged)-Human splicing factor 3b, subunit 2, 145kDa (SF3B2) |
CNY 6,392.00 |
|
| SC318368 | SF3B2 (untagged)-Human splicing factor 3b, subunit 2, 145kDa (SF3B2) |
CNY 17,200.00 |
