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SEC61A (SEC61A1) (NM_013336) Human Tagged ORF Clone
CAT＃: RG222756
- TrueORF®
SEC61A1 (tGFP-tagged) - Human Sec61 alpha 1 subunit (S. cerevisiae) (SEC61A1)
ORF Plasmid: DDK
"NM_013336" in other vectors (6)
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CNY 7,088.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ADTKD5; HNFJ4; HSEC61; SEC61; SEC61A |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG222756 representing NM_013336
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAATCAAATTTCTGGAAGTCATCAAGCCCTTCTGTGTCATCCTGCCGGAAATTCAGAAGCCAGAGA GGAAGATTCAGTTTAAGGAGAAAGTGCTGTGGACCGCTATCACCCTCTTTATCTTCTTAGTGTGCTGCCA GATTCCCCTGTTTGGGATCATGTCTTCAGATTCAGCTGACCCTTTCTATTGGATGAGAGTGATTCTAGCC TCTAACAGAGGCACATTGATGGAGCTAGGGATCTCTCCTATTGTCACGTCTGGCCTTATAATGCAACTCT TGGCTGGCGCCAAGATAATTGAAGTTGGTGACACCCCAAAAGACCGAGCTCTCTTCAACGGAGCCCAAAA GTTATTTGGCATGATCATTACTATCGGCCAGTCTATCGTGTATGTGATGACCGGGATGTATGGGGACCCT TCTGAAATGGGTGCTGGAATTTGCCTGCTAATCACCATTCAGCTCTTTGTTGCTGGCTTAATTGTCCTAC TTTTGGATGAACTCCTGCAAAAAGGATATGGCCTTGGCTCTGGTATTTCTCTCTTCATTGCAACTAACAT CTGTGAAACCATCGTATGGAAGGCATTCAGCCCCACTACTGTCAACACTGGCCGAGGAATGGAATTTGAA GGTGCTATCATCGCACTTTTCCATCTGCTGGCCACACGCACAGACAAGGTCCGAGCCCTTCGGGAGGCGT TCTACCGCCAGAATCTTCCCAACCTCATGAATCTCATCGCCACCATCTTTGTCTTTGCAGTGGTCATCTA TTTCCAGGGCTTCCGAGTGGACCTGCCAATCAAGTCGGCCCGCTACCGTGGCCAGTACAACACCTATCCC ATCAAGCTCTTCTATACGTCCAACATCCCCATCATCCTGCAGTCTGCCCTGGTGTCCAACCTTTATGTCA TCTCCCAAATGCTCTCAGCTCGCTTCAGTGGCAACTTGCTGGTCAGCCTGCTGGGCACCTGGTCGGACAC GTCTTCTGGGGGCCCAGCACGTGCTTATCCAGTTGGTGGCCTTTGCTATTACCTGTCCCCTCCAGAATCT TTTGGCTCCGTGTTAGAAGACCCGGTCCATGCAGTTGTATACATAGTGTTCATGCTGGGCTCCTGTGCAT TCTTCTCCAAAACGTGGATTGAGGTCTCAGGTTCCTCTGCCAAAGATGTTGCAAAGCAGCTGAAGGAGCA GCAGATGGTGATGAGAGGCCACCGAGAGACCTCCATGGTCCATGAACTCAACCGGTACATCCCCACAGCC GCGGCCTTTGGTGGGCTGTGCATCGGGGCCCTCTCGGTCCTGGCTGACTTCCTAGGCGCCATTGGGTCTG GAACCGGGATCCTGCTCGCAGTCACAATCATCTACCAGTACTTTGAGATCTTCGTTAAGGAGCAAAGCGA GGTTGGCAGCATGGGGGCCCTGCTCTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222756 representing NM_013336
Red=Cloning site Green=Tags(s) MAIKFLEVIKPFCVILPEIQKPERKIQFKEKVLWTAITLFIFLVCCQIPLFGIMSSDSADPFYWMRVILA SNRGTLMELGISPIVTSGLIMQLLAGAKIIEVGDTPKDRALFNGAQKLFGMIITIGQSIVYVMTGMYGDP SEMGAGICLLITIQLFVAGLIVLLLDELLQKGYGLGSGISLFIATNICETIVWKAFSPTTVNTGRGMEFE GAIIALFHLLATRTDKVRALREAFYRQNLPNLMNLIATIFVFAVVIYFQGFRVDLPIKSARYRGQYNTYP IKLFYTSNIPIILQSALVSNLYVISQMLSARFSGNLLVSLLGTWSDTSSGGPARAYPVGGLCYYLSPPES FGSVLEDPVHAVVYIVFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRETSMVHELNRYIPTA AAFGGLCIGALSVLADFLGAIGSGTGILLAVTIIYQYFEIFVKEQSEVGSMGALLF TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013336 |
| ORF Size | 1428 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013336.4 |
| RefSeq Size | 3656 bp |
| RefSeq ORF | 1431 bp |
| Locus ID | 29927 |
| UniProt ID | P61619 |
| Domains | secY |
| Protein Families | Transmembrane |
| Protein Pathways | Vibrio cholerae infection |
| Gene Summary | The protein encoded by this gene belongs to the SECY/SEC61- alpha family. It appears to play a crucial role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum. This protein found to be tightly associated with membrane-bound ribosomes, either directly or through adaptor proteins. This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also contains beta and gamma subunits. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222756 | SEC61A1 (Myc-DDK-tagged)-Human Sec61 alpha 1 subunit (S. cerevisiae) (SEC61A1) |
CNY 5,488.00 |
|
| RC222756L1 | Lenti ORF clone of Human Sec61 alpha 1 subunit (S. cerevisiae) (SEC61A1), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC222756L2 | Lenti ORF clone of Human Sec61 alpha 1 subunit (S. cerevisiae) (SEC61A1), mGFP tagged |
CNY 5,890.00 |
|
| RC222756L3 | Lenti ORF clone of Human Sec61 alpha 1 subunit (S. cerevisiae) (SEC61A1), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC222756L4 | Lenti ORF clone of Human Sec61 alpha 1 subunit (S. cerevisiae) (SEC61A1), mGFP tagged |
CNY 7,888.00 |
|
| SC115289 | SEC61A1 (untagged)-Human Sec61 alpha 1 subunit (S. cerevisiae) (SEC61A1) |
CNY 5,488.00 |
