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PIP5K3 (PIKFYVE) (NM_015040) Human Tagged ORF Clone
CAT＃: RG222618
- TrueORF®
PIKFYVE (tGFP-tagged) - Human phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant 2
ORF Plasmid: DDK
"NM_015040" in other vectors (2)
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CNY 22,990.00
货期*
12周
规格
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经常一起买 (5)
PIKFYVE mouse monoclonal antibody,clone OTI1D2
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG222618 representing NM_015040
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACAGATGATAAGACGTCCCCAACACTGGACTCTGCTAATGATTTGCCTCGATCTCCTACTAGTC CTTCTCATCTCACACACTTTAAACCTTTGACTCCTGATCAAGATGAGCCCCCTTTTAAATCAGCTTATAG TTCTTTTGTAAATCTCTTTCGTTTTAACAAAGAGAGAGCAGAAGGAGGCCAGGGAGAACAGCAGCCTTTG AGTGGAAGTTGGACCAGCCCTCAGCTCCCTTCGAGGACACAGTCTGTTAGGTCACCCACACCTTATAAAA AGCAGCTTAATGAGGAACTCCAGCGGCGCTCTTCAGCATTAGACACAAGAAGGAAAGCAGAACCTACCTT TGGAGGTCATGACCCTCGTACAGCTGTTCAGCTTCGAAGCCTCAGCACAGTATTAAAACGCCTCAAGGAA ATCATGGAGGGGAAAAGCCAGGATAGTGACCTGAAACAATACTGGATGCCAGATAGCCAATGTAAAGAGT GCTATGACTGTAGTGAGAAATTTACAACCTTTAGGCGCAGACACCATTGCCGACTATGTGGGCAGATTTT CTGCAGTCGTTGCTGTAATCAAGAAATCCCTGGAAAATTTATGGGCTATACAGGAGACCTCCGAGCTTGC ACATATTGTAGAAAAATAGCCTTAAGTTATGCTCATTCCACAGACAGTAATTCTATTGGGGAAGACTTGA ATGCTCTTTCAGATTCTGCTTGCTCTGTGTCTGTGCTTGATCCAAGTGAACCCCGAACACCTGTTGGGAG TAGGAAAGCCAGCCGTAACATATTTTTAGAGGATGATTTGGCCTGGCAAAGTTTGATTCATCCAGATTCC TCAAATACTCCTCTTTCAACAAGACTTGTATCTGTGCAAGAGGATGCTGGGAAATCTCCTGCTCGAAATA GATCAGCCAGCATTACTAACCTGTCACTGGATAGATCTGGTTCTCCTATGGTACCTTCATATGAGACATC TGTCAGTCCCCAGGCTAACCGAACATATGTTAGGACAGAGACCACTGAGGATGAACGCAAAATTCTTCTG GACAGTGTGCAGTTAAAAGACCTGTGGAAAAAAATCTGCCATCACAGCAGTGGAATGGAGTTTCAGGATC ACCGCTACTGGTTGAGAACGCATCCCAACTGCATTGTAGGAAAGGAATTAGTCAACTGGCTAATCCGAAA TGGGCATATTGCCACAAGGGCACAAGCTATAGCAATTGGACAAGCAATGGTTGATGGACGTTGGCTGGAT TGTGTTAGTCATCACGACCAGCTTTTCAGAGATGAGTATGCGCTGTATAGACCACTGCAGAGTACAGAAT TTTCTGAGACGCCTTCTCCCGACAGTGACTCAGTGAACTCCGTGGAAGGACACTCTGAGCCATCCTGGTT TAAAGACATAAAGTTTGATGACAGTGACACAGAACAGATAGCTGAAGAAGGTGACGATAATTTGGCTAAT TCTGCCAGTCCTAGCAAGCGCACATCAGTCAGCAGTTTCCAGTCCACAGTGGACAGTGACTCAGCCGCTT CTATCAGCCTGAACGTGGAGCTGGACAACGTGAACTTCCATATCAAGAAGCCCTCCAAGTACCCACATGT GCCCCCTCACCCTGCTGACCAAAAAGAGTATTTGATTTCTGACACTGGAGGACAACAGCTCTCAATAAGT GACGCTTTCATCAAAGAATCCTTATTTAATCGCCGAGTAGAGGAAAAATCCAAAGAGCTGCCTTTCACAC CTTTGGGCTGGCATCATAACAACCTGGAGCTCCTGAGGGAGGAGAATGGGGAGAAACAAGCCATGGAGAG GTTGCTTTCAGCTAATCATAACCACATGATGGCACTACTCCAGCAGTTGCTCCATAGTGACTCACTGTCA TCATCTTGGAGGGACATCATCGTGTCATTGGTCTGCCAGGTTGTTCAGACAGTCCGACCTGATGTCAAGA ACCAGGATGATGACATGGATATCCGTCAGTTTGTCCACATCAAAAAAATCCCAGGTGGAAAGAAGTTTGA TTCTGTGGTTGTCAATGGCTTTGTTTGTACCAAGAACATTGCACATAAAAAGATGAGTTCTTGTATTAAA AACCCCAAAATTCTTCTGTTGAAGTGTTCCATTGAGTATCTCTACAGAGAAGAAACTAAGTTTACTTGCA TTGATCCTATTGTGCTTCAGGAAAGGGAATTCTTGAAGAATTATGTCCAGCGAATAGTTGATGTTCGACC CACCTTGGTTCTTGTTGAGAAAACAGTGTCTCGGATTGCCCAGGACATGTTATTGGAACATGGCATTACT TTGGTCATTAATGTAAAGTCACAAGTTTTGGAACGAATCAGTCGAATGACCCAAGGTGATTTAGTGATGT CAATGGACCAGCTGCTTACGAAACCACACCTGGGCACTTGTCACAAATTTTATATGCAGATATTTCAGTT GCCTAATGAACAAACCAAGACACTGATGTTTTTTGAAGGTTGTCCACAGCACCTAGGCTGTACAATCAAG CTAAGAGGAGGCTCTGATTATGAGCTGGCTCGAGTTAAGGAGATCCTAATATTTATGATCTGTGTTGCTT ATCATTCTCAACTAGAAATATCCTTTCTCATGGATGAATTTGCTATGCCTCCCACATTAATGCAAAACCC TTCATTCCATTCCCTGATTGAGGGACGAGGGCATGAGGGGGCTGTCCAAGAGCAGTACGGTGGAGGTTCC ATCCCCTGGGATCCTGACATCCCTCCTGAGTCTCTGCCCTGTGATGATAGCAGTTTGCTGGAATTGAGGA TTGTGTTTGAGAAGGGTGAGCAGGAAAATAAAAATCTTCCGCAGGCTGTTGCCTCTGTGAAGCATCAAGA ACATAGCACAACAGCTTGCCCGGCGGGTCTCCCTTGTGCTTTCTTTGCACCTGTACCGGAATCATTGTTG CCACTCCCTGTGGATGACCAACAAGATGCTTTAGGCAGCGAGCAGCCAGAGACTTTGCAGCAAACAGTTG TGCTGCAGGATCCCAAAAGCCAGATAAGAGCCTTTAGAGACCCTCTACAGGATGACACTGGATTATATGT TACTGAGGAAGTCACCTCCTCTGAAGATAAACGAAAGACTTATTCTTTGGCCTTTAAGCAGGAATTAAAA GATGTGATCCTCTGTATCTCCCCAGTAATCACATTCCGAGAACCCTTTCTTTTAACTGAAAAGGGGATGA GATGCTCTACCCGAGATTATTTTGCAGAGCAGGTTTACTGGTCTCCTCTCCTCAATAAAGAATTCAAAGA AATGGAGAACAGGAGGAAGAAACAGCTGCTCAGGGATCTCTCTGGACTTCAGGGCATGAATGGAAGTATT CAGGCCAAGTCTATTCAAGTCTTACCCTCACATGAGCTAGTGAGCACTAGAATTGCTGAGCATCTGGGCG ATAGCCAGAGCTTGGGTAGAATGCTGGCCGATTATCGAGCCAGAGGAGGAAGAATTCAGCCCAAAAATTC AGACCCTTTTGCTCATTCAAAGGATGCATCAAGTACTTCAAGTGGCCAATCAGGAAGCAAAAATGAGGGT GATGAAGAGAGAGGGCTTATTCTGAGTGATGCTGTGTGGTCAACAAAGGTGGACTGTCTGAATCCCATTA ATCACCAGAGACTTTGTGTGCTCTTCAGCAGCTCTTCTGCCCAGTCCAGCAATGCTCCTAGTGCCTGTGT CAGTCCTTGGATTGTAACAATGGAATTTTATGGAAAGAATGATCTTACATTAGGAATATTTTTAGAGAGA TACTGTTTCAGGCCTTCTTATCAGTGTCCAAGCATGTTCTGTGATACCCCCATGGTACATCATATTCGGC GCTTTGTTCATGGCCAAGGCTGTGTGCAGATAATCCTGAAGGAGTTGGATTCTCCAGTACCTGGATATCA GCATACAATTCTTACATATTCCTGGTGTAGAATCTGCAAACAGGTAACACCAGTTGTTGCTCTTTCCAAT GAGTCCTGGTCTATGTCATTTGCAAAATACCTTGAACTTAGGTTTTATGGGCACCAGTATACTCGCAGAG CCAACGCTGAGCCCTGTGGTCACTCCATCCATCATGATTATCACCAGTATTTCTCCTATAACCAGATGGT GGCGTCTTTCAGTTATTCTCCCATTCGGCTTCTTGAAGTATGTGTTCCACTCCCCAAAATATTCATTAAG CGTCAGGCCCCATTAAAAGTGTCCCTTCTTCAGGATCTGAAGGACTTCTTTCAAAAAGTTTCACAGGTAT ATGTTGCCATTGATGAAAGACTTGCATCTTTGAAAACTGATACATTTAGTAAAACAAGAGAGGAAAAAAT GGAAGATATTTTTGCACAGAAAGAGATGGAAGAAGGTGAGTTCAAGAACTGGATTGAGAAGATGCAAGCA AGGCTCATGTCTTCCTCTGTAGATACCCCTCAGCAACTGCAGTCGGTCTTTGAGTCACTCATTGCCAAGA AACAAAGTCTCTGTGAAGTGCTGCAAGCTTGGAATAACAGGTTGCAGGACCTTTTCCAACAGGAAAAGGG TAGAAAGAGACCTTCAGTTCCTCCAAGTCCTGGAAGACTGAGACAAGGGGAAGAAAGCAAGATAAGTGCG ATGGATGCATCTCCACGGAATATTTCTCCAGGACTTCAGAATGGAGAAAAAGAGGATCGCTTCTTAACAA CTTTGTCCAGCCAGAGCTCCACCAGTTCTACTCATCTCCAATTGCCTACGCCACCTGAAGTCATGTCTGA ACAGTCAGTGGGAGGGCCCCCTGAGCTAGATACAGCCAGCAGTTCCGAAGATGTGTTTGATGGGCATTTG CTGGGATCCACAGACAGCCAAGTGAAGGAAAAGTCAACCATGAAAGCCATCTTTGCAAATTTGCTTCCAG GAAATAGCTATAATCCTATTCCATTTCCTTTTGATCCAGATAAACACTACTTAATGTATGAACATGAACG AGTGCCCATTGCAGTCTGCGAGAAGGAACCCAGCTCCATCATTGCTTTTGCTCTCAGTTGTAAAGAATAC CGAAATGCCTTAGAGGAATTGTCTAAAGCGACTCAGTGGAACAGTGCCGAAGAAGGGCTTCCAACAAATA GTACTTCAGATAGCAGACCAAAGAGTAGCAGCCCTATCAGATTACCTGAAATGAGTGGAGGACAGACAAA TCGTACAACAGAAACAGAACCACAACCAACCAAAAAGGCTTCTGGAATGTTGTCCTTCTTCAGAGGGACA GCAGGGAAAAGCCCCGATCTCTCTTCCCAGAAGAGAGAGACCTTACGTGGAGCAGATAGTGCTTACTACC AGGTTGGGCAGACGGGCAAGGAGGGGACCGAGAATCAAGGCGTTGAGCCTCAAGATGAAGTAGATGGAGG AGATACACAAAAGAAGCAACTCATAAATCCTCATGTGGAACTTCAATTTTCAGATGCTAATGCCAAGTTT TACTGTCGGCTCTACTATGCGGGAGAGTTTCATAAGATGCGTGAAGTGATTCTGGACAGCAGTGAAGAAG ATTTCATTCGTTCCCTCTCCCACTCATCACCCTGGCAGGCCCGGGGAGGCAAATCAGGAGCTGCCTTCTA TGCAACTGAGGATGATAGATTTATTTTGAAGCAAATGCCTCGTCTGGAAGTCCAGTCCTTCCTCGACTTT GCACCACATTACTTCAATTATATTACAAATGCTGTTCAACAAAAGAGGCCCACGGCGTTGGCCAAAATTC TTGGAGTTTACAGAATTGGTTATAAGAACTCTCAGAACAACACTGAGAAGAAGTTAGATCTCCTTGTCAT GGAAAATCTTTTCTACGGGAGAAAGATGGCACAGGTTTTTGATTTGAAGGGCTCTCTTAGGAATCGGAAT GTAAAAACTGACACTGGAAAAGAGAGTTGTGATGTGGTCCTGCTAGATGAAAATCTCCTAAAGATGGTTC GAGACAACCCTCTATATATTCGTTCTCATTCCAAAGCTGTGCTGAGAACCTCGATCCATAGTGACTCCCA TTTCCTTTCTAGCCACCTCATTATAGATTATTCTTTGCTGGTTGGGCGAGATGATACTAGCAATGAGCTA GTAGTTGGAATTATAGATTATATTCGAACATTTACATGGGACAAAAAGCTTGAGATGGTTGTGAAATCAA CAGGAATTTTAGGTGGACAAGGTAAAATGCCAACAGTGGTGTCTCCGGAGTTGTACAGGACTAGGTTTTG TGAGGCAATGGACAAGTATTTCCTAATGGTACCAGACCACTGGACAGGCTTGGGTCTGAATTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222618 representing NM_015040
Red=Cloning site Green=Tags(s) MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPL SGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKE IMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRAC TYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDS SNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILL DSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLD CVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLAN SASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDTGGQQLSIS DAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLS SSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIK NPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGIT LVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIK LRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGS IPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLL PLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELK DVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSI QAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEG DEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLER YCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSN ESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIK RQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQA RLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISA MDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHL LGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEY RNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGMLSFFRGT AGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKF YCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDF APHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRN VKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNEL VVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015040 |
| ORF Size | 6294 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015040.4 |
| RefSeq Size | 9901 bp |
| RefSeq ORF | 6297 bp |
| Locus ID | 200576 |
| UniProt ID | Q9Y2I7 |
| Protein Families | Druggable Genome |
| Protein Pathways | Endocytosis, Fc gamma R-mediated phagocytosis, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system, Regulation of actin cytoskeleton |
| Gene Summary | Phosphorylated derivatives of phosphatidylinositol (PtdIns) regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. Humans have multiple PtdIns proteins that differ by the degree and position of phosphorylation of the inositol ring. This gene encodes an enzyme (PIKfyve; also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) that phosphorylates the D-5 position in PtdIns and phosphatidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns(3,5)biphosphate. The D-5 position also can be phosphorylated by type I PtdIns4P-5-kinases (PIP5Ks) that are encoded by distinct genes and preferentially phosphorylate D-4 phosphorylated PtdIns. In contrast, PIKfyve preferentially phosphorylates D-3 phosphorylated PtdIns. In addition to being a lipid kinase, PIKfyve also has protein kinase activity. PIKfyve regulates endomembrane homeostasis and plays a role in the biogenesis of endosome carrier vesicles from early endosomes. Mutations in this gene cause corneal fleck dystrophy (CFD); an autosomal dominant disorder characterized by numerous small white flecks present in all layers of the corneal stroma. Histologically, these flecks appear to be keratocytes distended with lipid and mucopolysaccharide filled intracytoplasmic vacuoles. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, May 2010] |
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