United States
Germany
Japan
United Kingdom
China
C8 (C8G) (NM_000606) Human Tagged ORF Clone
CAT＃: RG222236
- TrueORF®
C8G (tGFP-tagged) - Human complement component 8, gamma polypeptide (C8G)
ORF Plasmid: DDK
"NM_000606" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C8C |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG222236 representing NM_000606
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCCCCCTGGGACTGCGACCCTCTTGACTCTGCTCCTGGCAGCTGGCTCGCTGGGCCAGAAGCCTC AGAGGCCACGCCGGCCCGCATCCCCCATCAGCACCATCCAGCCCAAGGCCAATTTTGATGCTCAGCAGTT TGCAGGGACCTGGCTCCTTGTGGCTGTGGGCTCCGCTTGCCGTTTCCTGCAGGAGCAGGGCCACCGGGCC GAGGCCACCACACTGCATGTGGCTCCCCAGGGCACAGCCATGGCTGTCAGTACCTTCCGAAAGCTGGATG GGATCTGCTGGCAGGTGCGCCAGCTCTATGGAGACACAGGGGTCCTCGGCCGCTTCCTGCTTCAAGCCCG AGGCGCCCGAGGGGCTGTGCACGTGGTTGTCGCTGAGACCGACTACCAGAGTTTCGCTGTCCTGTACCTG GAGCGGGCGGGGCAGCTGTCAGTGAAGCTCTACGCCCGCTCGCTCCCTGTGAGCGACTCGGTCCTGAGTG GGTTTGAGCAGCGGGTCCAGGAGGCCCACCTGACTGAGGACCAGATCTTCTACTTCCCCAAGTACGGCTT CTGCGAGGCTGCAGACCAGTTCCACGTCCTGGACGAAGTGAGGAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222236 representing NM_000606
Red=Cloning site Green=Tags(s) MLPPGTATLLTLLLAAGSLGQKPQRPRRPASPISTIQPKANFDAQQFAGTWLLVAVGSACRFLQEQGHRA EATTLHVAPQGTAMAVSTFRKLDGICWQVRQLYGDTGVLGRFLLQARGARGAVHVVVAETDYQSFAVLYL ERAGQLSVKLYARSLPVSDSVLSGFEQRVQEAHLTEDQIFYFPKYGFCEAADQFHVLDEVRR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000606 |
| ORF Size | 606 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000606.1, NP_000597.1 |
| RefSeq Size | 857 bp |
| RefSeq ORF | 609 bp |
| Locus ID | 733 |
| UniProt ID | P07360 |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus |
| Gene Summary | The protein encoded by this gene belongs to the lipocalin family. It is one of the three subunits that constitutes complement component 8 (C8), which is composed of a disulfide-linked C8 alpha-gamma heterodimer and a non-covalently associated C8 beta chain. C8 participates in the formation of the membrane attack complex (MAC) on bacterial cell membranes. While subunits alpha and beta play a role in complement-mediated bacterial killing, the gamma subunit is not required for the bactericidal activity. [provided by RefSeq, Jul 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222236 | C8G (Myc-DDK-tagged)-Human complement component 8, gamma polypeptide (C8G) |
CNY 2,400.00 |
|
| RC222236L1 | Lenti ORF clone of Human complement component 8, gamma polypeptide (C8G), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222236L2 | Lenti ORF clone of Human complement component 8, gamma polypeptide (C8G), mGFP tagged |
CNY 4,800.00 |
|
| RC222236L3 | Lenti ORF clone of Human complement component 8, gamma polypeptide (C8G), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222236L4 | Lenti ORF clone of Human complement component 8, gamma polypeptide (C8G), mGFP tagged |
CNY 5,890.00 |
|
| SC300107 | C8G (untagged)-Human complement component 8, gamma polypeptide (C8G) |
CNY 3,990.00 |
