IFNE1 (IFNE) (NM_176891) Human Tagged ORF Clone

CAT#: RG222153

  • TrueORF®

IFNE (tGFP-tagged) - Human interferon, epsilon (IFNE)

ORF Plasmid: DDK tGFP



  "NM_176891" in other vectors (6)

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CNY 4,000.00

CNY 4,370.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal Anti-IFNE Antibody
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms IFN-E; IFNE1; IFNT1; INFE1; PRO655
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG222153 representing NM_176891
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATTATCAAGCACTTCTTTGGAACTGTGTTGGTGCTGCTGGCCTCTACCACTATCTTCTCTCTAGATT
TGAAACTGATTATCTTCCAGCAAAGACAAGTGAATCAAGAAAGTTTAAAACTCTTGAATAAGTTGCAAAC
CTTGTCAATTCAGCAGTGTCTACCACACAGGAAAAACTTTCTGCTTCCTCAGAAGTCTTTGAGTCCTCAG
CAGTACCAAAAAGGACACACTCTGGCCATTCTCCATGAGATGCTTCAGCAGATCTTCAGCCTCTTCAGGG
CAAATATTTCTCTGGATGGTTGGGAGGAAAACCACACGGAGAAATTCCTCATTCAACTTCATCAACAGCT
AGAATACCTAGAAGCACTCATGGGACTGGAAGCAGAGAAGCTAAGTGGTACTTTGGGTAGTGATAACCTT
AGATTACAAGTTAAAATGTACTTCCGAAGGATCCATGATTACCTGGAAAACCAGGACTACAGCACCTGTG
CCTGGGCCATTGTCCAAGTAGAAATCAGCCGATGTCTGTTCTTTGTGTTCAGTCTCACAGAAAAACTGAG
CAAACAAGGAAGACCCTTGAACGACATGAAGCAAGAGCTTACTACAGAGTTTAGAAGCCCGAGG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG222153 representing NM_176891
Red=Cloning site Green=Tags(s)

MIIKHFFGTVLVLLASTTIFSLDLKLIIFQQRQVNQESLKLLNKLQTLSIQQCLPHRKNFLLPQKSLSPQ
QYQKGHTLAILHEMLQQIFSLFRANISLDGWEENHTEKFLIQLHQQLEYLEALMGLEAEKLSGTLGSDNL
RLQVKMYFRRIHDYLENQDYSTCAWAIVQVEISRCLFFVFSLTEKLSKQGRPLNDMKQELTTEFRSPR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_176891
ORF Size 624 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_176891.4
RefSeq Size 1502 bp
RefSeq ORF 627 bp
Locus ID 338376
UniProt ID Q86WN2
Protein Families Druggable Genome, Transmembrane
Protein Pathways Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, RIG-I-like receptor signaling pathway
Gene Summary Type I interferon required for maintaining basal levels of IFN-regulated genes, including 2'-5'-oligoadenylate synthetase, IRF7 and ISG15, in the female reproductive tract. Directly mediates protection against viral and bacterial genital infections (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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