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GRF2 (RAPGEF1) (NM_005312) Human Tagged ORF Clone
CAT＃: RG221671
- TrueORF®
RAPGEF1 (tGFP-tagged) - Human Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 1
ORF Plasmid: DDK
"NM_005312" in other vectors (4)
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CNY 11,780.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C3G; GRF2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG221671 representing NM_005312
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACACAGACTCTCAGCGTTCTCATCTCTCTTCCTTCACCATGAAGCTGATGGACAAATTCCACTCAC CCAAAATCAAGAGAACGCCATCAAAGAAGGGAAAACCAGCTGAGGTGTCCGTAAAGATTCCAGAGAAGCC TGTGAACAAAGAGGCAACAGACAGATTTCTACCAGAGGGCTACCCTCTCCCCTTGGATCTGGAGCAGCAG GCAGTAGAATTTATGTCCACCAGTGCTGTGGCTTCCAGGTCTCAAAGGCAGAAGAACCTGAGCTGGCTGG AGGAGAAAGAGAAGGAAGTTGTCAGTGCCCTGCGCTACTTTAAGACCATTGTGGACAAAATGGCAATTGA TAAGAAGGTACTGGAGATGCTTCCAGGGTCAGCCAGCAAGGTGCTGGAGGCCATCTTACCCCTGGTGCAG AACGATCCTCGAATTCAGCACAGCTCAGCCCTCTCTTCCTGCTATAGCCGAGTGTACCAAAGCCTCGCCA ACCTCATTCGCTGGTCTGACCAAGTGATGCTGGAAGGCGTGAACTCAGAAGACAAGGAGATGGTGACGAC TGTGAAGGGGGTCATCAAGGCTGTGCTGGATGGAGTGAAGGAGCTGGTCAGGCTCACCATCGAGAAGCAG GGACGTCCGTCTCCGACGAGCCCCGTGAAGCCCAGTTCCCCTGCCAGCAAGCCTGATGGCCCAGCAGAGC TCCCCCTGACAGACCGCGAGGTAGAGATCCTAAACAAGACGACTGGGATGTCACAGTCAACTGAGCTCCT CCCAGATGCCACGGATGAAGAGGTCGCGCCCCCCAAGCCTCCTCTGCCTGGCATTCGGGTGGTTGATAAT AGTCCTCCACCAGCATTGCCACCCAAGAAAAGACAGTCGGCGCCGTCCCCTACCCGAGTGGCTGTGGTGG CCCCCATGAGCCGAGCCACCAGTGGCTCCAGTTTGCCTGTTGGAATCAATAGGCAGGATTTTGATGTTGA CTGTTACGCACAGAGGCGACTGTCAGGAGGCAGCCACTCATATGGTGGAGAGTCGCCCCGCCTCTCCCCC TGCAGCAGCATAGGCAAGCTCAGCAAGTCAGACGAGCAGCTGTCCTCTCTGGACAGGGACAGTGGGCAGT GCTCCCGGAACACAAGCTGTGAAACACTAGACCACTATGATCCCGACTATGAATTCCTCCAGCAAGACCT CTCTAACGCAGACCAGATACCTCAGCAGACGGCCTGGAACCTTAGCCCGTTGCCAGAGTCTTTGGGGGAG TCTGGGTCTCCATTTCTTGGCCCTCCTTTCCAGCTGCCTCTTGGCGGCCATCCCCAGCCAGACGGACCTC TGGCCCCAGGGCAGCAGACAGATACGCCACCTGCTCTCCCCGAGAAGAAGCGCAGGAGCGCAGCCTCCCA GACGGCGGACGGCTCTGGCTGCAGGGTGTCCTACGAGCGGCATCCCTCGCAGTATGACAACATCTCTGGG GAGGACCTGCAGAGCACAGCCCCGATCCCATCCGTCCCCTACGCGCCCTTTGCTGCTATTCTGCCCTTTC AGCATGGAGGTTCCTCAGCCCCTGTCGAATTTGTGGGTGATTTTACTGCTCCTGAGTCAACCGGTGACCC AGAAAAACCACCTCCTCTACCAGAGAAGAAAAACAAACACATGCTGGCCTACATGCAGTTGCTGGAGGAC TACTCGGAGCCGCAGCCCTCTATGTTCTACCAGACGCCACAGAACGAGCACATCTACCAGCAGAAGAACA AGCTCCTCATGGAGGTATACGGCTTCAGCGACTCCTTCAGTGGGGTGGACTCCGTGCAGGAGCTGGCCCC GCCGCCCGCCCTACCCCCCAAGCAGCGGCAGCTGGAGCCACCGGCTGGGAAAGACGGACATCCCAGAGAT CCCTCAGCGGTCAGCGGCGTCCCTGGGAAGGACAGCAGAGACGGCAGTGAGAGGGCCCCAAAGTCACCAG ATGCTCTGGAGTCGGCTCAGTCGGAGGAGGAAGTGGACGAGCTGTCCCTCATTGACCACAACGAAATTAT GTCCAGGCTGACGCTCAAGCAGGAGGGTGATGACGGGCCGGACGTCCGCGGAGGATCTGGGGACATCTTA CTGGTCCATGCTACTGAGACTGACAGGAAAGATTTGGTGTTGTACTGCGAGGCATTCCTGACCACCTACA GGACCTTCATCTCCCCAGAGGAGCTCATCAAGAAGCTGCAGTACAGATATGAGAAATTCTCTCCCTTTGC CGACACATTCAAGAAGCGCGTCAGCAAGAACACGTTCTTCGTGCTGGTACGGGTGGTGGATGAGCTCTGC CTGGTGGAGTTGACAGAAGAGATCCTGAAGCTGCTGATGGAACTGGTCTTCCGCCTGGTGTGCAATGGGG AGCTGAGCCTGGCCCGTGTGCTCCGGAAGAACATCCTGGACAAGGTGGACCAGAAGAAGCTACTCAGGTG TGCCACCTCCAGCCAGCCCCTGGCAGCCCGGGGGGTAGCAGCCAGGCCGGGGACCTTGCACGACTTTCAC AGCCATGAGATAGCGGAGCAGCTAACGCTGCTGGATGCTGAGCTCTTCTATAAAATAGAGATTCCTGAGG TTTTGCTTTGGGCAAAAGAGCAGAATGAGGAGAAGAGCCCCAACTTGACCCAGTTCACGGAGCACTTCAA CAACATGTCCTACTGGGTCCGGTCCATAATCATGTTACAGGAAAAGGCCCAGGACAGGGAACGGCTGCTC TTGAAGTTCATCAAGATCATGAAGCACTTGCGGAAGCTGAATAACTTCAACTCCTACTTGGCCATCCTCT CTGCCCTGGACTCGGCGCCCATCCGCAGGCTGGAGTGGCAGAAGCAGACTTCAGAGGGCCTGGCCGAGTA CTGCACACTGATCGACAGCTCGTCCTCCTTCCGAGCCTACCGGGCCGCCCTCTCGGAGGTGGAACCGCCG TGCATCCCGTACCTGGGGCTGATCCTGCAGGACCTGACCTTCGTTCACCTGGGAAACCCAGACTACATCG ACGGGAAAGTGAACTTCTCCAAGCGGTGGCAGCAGTTCAACATCCTCGACAGCATGCGCTGCTTCCAGCA GGCGCACTATGACATGCGGAGGAACGACGACATTATAAACTTCTTCAATGACTTCAGTGACCACCTGGCT GAGGAGGCCCTATGGGAACTGTCTCTGAAAATTAAACCCAGGAACATAACAAGGAGAAAAACAGACCGGG AAGAGAAGACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221671 representing NM_005312
Red=Cloning site Green=Tags(s) MDTDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQ AVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQ NDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQ GRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDN SPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSP CSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGE SGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISG EDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLED YSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLEPPAGKDGHPRD PSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDIL LVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELC LVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFH SHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLL LKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPP CIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLA EEALWELSLKIKPRNITRRKTDREEKT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005312 |
| ORF Size | 3231 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005312.4 |
| RefSeq Size | 6121 bp |
| RefSeq ORF | 3234 bp |
| Locus ID | 2889 |
| UniProt ID | Q13905 |
| Domains | RasGEFN, RasGEF |
| Protein Families | Druggable Genome |
| Protein Pathways | Focal adhesion, Insulin signaling pathway, Neurotrophin signaling pathway, Renal cell carcinoma |
| Gene Summary | This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221671 | RAPGEF1 (Myc-DDK-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 1 |
CNY 10,740.00 |
|
| RC221671L3 | Lenti-ORF clone of RAPGEF1 (Myc-DDK-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 1 |
CNY 12,640.00 |
|
| RC221671L4 | Lenti-ORF clone of RAPGEF1 (mGFP-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 1 |
CNY 12,640.00 |
|
| SC315151 | RAPGEF1 (untagged)-Human Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 1 |
CNY 20,710.00 |
