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KCNU1 (NM_001031836) Human Tagged ORF Clone
CAT＃: RG221094
- TrueORF®
KCNU1 (tGFP-tagged) - Human potassium channel, subfamily U, member 1 (KCNU1)
ORF Plasmid: DDK
"NM_001031836" in other vectors (4)
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CNY 12,640.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG221094 representing NM_001031836
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCAGACTAAGCTACGAAATGAAACTTGGGAAGACTTGCCAAAAATGTCCTGCACAACTGAGATCC AAGCAGCATTCATTCTCTCTTCCTTTGTGACCTTCTTCAGTGGACTCATCATCCTGTTGATCTTCAGGCT GATCTGGAGATCTGTTAAAAAATGGCAAATCATCAAGGGAACAGGAATTATCTTGGAACTGTTCACATCA GGTACCATCGCTAGGAGCCATGTAAGAAGCCTCCACTTCCAGGGACAATTTCGTGATCATATAGAAATGT TGCTTTCAGCCCAGACCTTTGTGGGGCAAGTGTTGGTGATCCTTGTCTTTGTACTAAGCATTGGGTCTCT TATAATCTATTTCATCAATTCTGCTGACCCTGTTGGAAGCTGTTCATCATATGAAGACAAAACCATTCCT ATTGATTTGGTTTTCAATGCTTTCTTTAGTTTCTATTTTGGATTGAGGTTTATGGCAGCTGATGACAAGA TCAAGTTCTGGCTGGAGATGAATTCAATCGTAGACATCTTTACCATCCCACCAACCTTTATTTCTTATTA TTTGAAGAGCAATTGGCTAGGTTTAAGGTTCCTAAGAGCCTTGCGCCTGCTAGAACTCCCTCAAATCTTG CAAATTCTACGAGCCATCAAGACCAGTAACTCAGTGAAGTTTTCCAAACTGCTGTCAATAATTCTCAGTA CCTGGTTCACAGCTGCGGGATTCATTCACCTGGTGGAAAATTCTGGTGATCCCTGGCTCAAAGGTAGAAA TTCACAGAATATATCATATTTTGAGTCAATTTACCTGGTCATGGCAACAACGTCAACCGTTGGATTTGGA GATGTGGTAGCCAAGACATCCTTAGGACGGACCTTCATCATGTTCTTCACACTGGGGAGTTTGATATTAT TTGCGAACTATATACCTGAAATGGTGGAACTGTTTGCTAACAAGAGGAAATACACCAGTTCCTATGAAGC ACTCAAAGGAAAGAAGTTTATTGTGGTCTGTGGAAACATCACTGTGGACAGTGTGACCGCTTTCCTGAGG AATTTCCTCCGCGACAAGTCAGGAGAGATCAACACTGAAATTGTTTTCCTGGGAGAAACCCCTCCTTCTT TGGAACTTGAAACCATATTTAAATGCTACTTGGCCTACACAACGTTCATTTCTGGATCTGCAATGAAGTG GGAGGATCTGAGGCGAGTTGCGGTGGAATCTGCAGAGGCATGCCTGATTATAGCCAATCCTTTGTGCAGT GATTCCCATGCTGAAGATATTTCCAACATTATGAGGGTGCTCTCTATCAAGAACTATGATTCTACCACCA GAATCATCATACAGATACTGCAATCCCATAACAAGGTTTATCTGCCAAAGATTCCCAGCTGGAACTGGGA CACCGGAGACAACATCATCTGCTTTGCTGAATTAAAACTTGGATTTATCGCCCAAGGCTGTTTGGTGCCA GGCTTGTGTACCTTCCTAACATCTCTATTTGTGGAGCAAAACAAAAAGGTTATGCCTAAACAGACCTGGA AGAAACACTTCTTGAATAGCATGAAAAACAAAATTCTGACCCAACGTCTCTCTGATGACTTTGCTGGAAT GAGCTTTCCTGAAGTTGCCCGGCTCTGCTTTCTGAAGATGCACCTCCTGTTGATAGCCATCGAATACAAG TCCCTCTTTACGGATGGTTTCTGTGGTCTGATACTAAATCCACCTCCACAAGTGAGGATACGTAAGAACA CATTAGGGTTCTTTATTGCTGAAACTCCAAAGGACGTCAGAAGAGCCTTGTTTTACTGTTCAGTCTGTCA TGATGATGTGTTCATTCCTGAGCTAATTACAAACTGTGGCTGCAAAAGCAGAAGCCGGCAGCACATCACA GTGCCATCGGTAAAGAGAATGAAAAAATGTCTGAAGGGAATCTCCTCTCGTATATCAGGGCAGGATTCTC CGCCAAGGGTATCTGCAAGCACTTCGAGCATATCAAACTTCACCACCAGGACTCTTCAACATGATGTAGA ACAAGATTCTGACCAGCTTGATAGCAGTGGGATGTTTCACTGGTGCAAACCAACCTCTTTGGACAAGGTG ACTCTGAAACGAACTGGCAAGTCAAAGTATAAGTTTCGGAACCATATTGTAGCATGTGTATTTGGAGATG CCCACTCAGCCCCGATGGGGCTTCGGAACTTTGTAATGCCCTTGAGAGCCAGCAACTATACCAGGAAGGA GCTGAAGGACATAGTGTTCATTGGGTCTCTGGACTATCTACAGAGAGAATGGCGATTTCTCTGGAATTTT CCCCAGATATACATTCTGCCTGGATGTGCACTTTATTCTGGAGACCTCCATGCGGCCAACATAGAGCAAT GCTCCATGTGTGCTGTCTTGTCCCCCCCACCCCAGCCATCAAGCAACCAGACTTTGGTAGACACAGAAGC CATCATGGCAACCCTCACCATCGGATCCTTGCAAATTGACTCCTCCTCTGACCCGTCACCCTCAGTGTCA GAGGAGACTCCAGGTTACACAAATGGACATAATGAGAAATCAAACTGCCGAAAAGTCCCTATCCTTACTG AACTGAAAAATCCTTCCAACATTCACTTTATTGAACAGCTTGGTGGACTGGAAGGGTCCCTCCAAGAAAC AAATCTGCATCTCAGCACTGCCTTTTCTACGGGCACTGTTTTTTCCGGCAGCTTCTTGGATTCTCTGCTG GCCACGGCCTTCTACAATTATCATGTCCTGGAATTGCTTCAGATGCTGGTGACAGGAGGAGTAAGTTCTC AGCTGGAACAACATTTAGATAAGGATAAAGTCTATGGTGTGGCAGATAGCTGCACGTCGCTCTTGTCTGG AAGAAACCGGTGTAAGCTGGGGCTTCTGTCCTTACACGAAACCATTTTATCAGACGTTAATCCAAGAAAC ACCTTTGGACAACTGTTCTGTGGCTCATTAGATCTTTTTGGAATCCTGTGTGTTGGCTTATACCGAATAA TTGATGAAGAGGAGCTCAACCCAGAAAACAAAAGGTTTGTGATCACCCGGCCAGCCAATGAGTTCAAGCT GCTGCCTTCAGATCTTGTGTTTTGTGCCATACCCTTCAGCACTGCTTGTTATAAAAGGAATGAAGAGTTC TCATTGCAAAAGTCATATGAAATTGTAAATAAAGCATCACAGACAACAGAGACACATTCAGACACAAATT GTCCTCCCACCATTGATTCAGTTACTGAGACATTGTATTCACCAGTCTATTCTTACCAGCCGAGAACTAA CTCCCTCTCTTTTCCTAAGCAAATAGCATGGAATCAGAGTAGAACAAACAGTATTATATCATCTCAGATA CCTTTAGGTGACAATGCAAAAGAAAATGAAAGGAAAACTTCAGATGAGGTTTATGATGAGGATCCCTTTG CATATTCAGAGCCACTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221094 representing NM_001031836
Red=Cloning site Green=Tags(s) MFQTKLRNETWEDLPKMSCTTEIQAAFILSSFVTFFSGLIILLIFRLIWRSVKKWQIIKGTGIILELFTS GTIARSHVRSLHFQGQFRDHIEMLLSAQTFVGQVLVILVFVLSIGSLIIYFINSADPVGSCSSYEDKTIP IDLVFNAFFSFYFGLRFMAADDKIKFWLEMNSIVDIFTIPPTFISYYLKSNWLGLRFLRALRLLELPQIL QILRAIKTSNSVKFSKLLSIILSTWFTAAGFIHLVENSGDPWLKGRNSQNISYFESIYLVMATTSTVGFG DVVAKTSLGRTFIMFFTLGSLILFANYIPEMVELFANKRKYTSSYEALKGKKFIVVCGNITVDSVTAFLR NFLRDKSGEINTEIVFLGETPPSLELETIFKCYLAYTTFISGSAMKWEDLRRVAVESAEACLIIANPLCS DSHAEDISNIMRVLSIKNYDSTTRIIIQILQSHNKVYLPKIPSWNWDTGDNIICFAELKLGFIAQGCLVP GLCTFLTSLFVEQNKKVMPKQTWKKHFLNSMKNKILTQRLSDDFAGMSFPEVARLCFLKMHLLLIAIEYK SLFTDGFCGLILNPPPQVRIRKNTLGFFIAETPKDVRRALFYCSVCHDDVFIPELITNCGCKSRSRQHIT VPSVKRMKKCLKGISSRISGQDSPPRVSASTSSISNFTTRTLQHDVEQDSDQLDSSGMFHWCKPTSLDKV TLKRTGKSKYKFRNHIVACVFGDAHSAPMGLRNFVMPLRASNYTRKELKDIVFIGSLDYLQREWRFLWNF PQIYILPGCALYSGDLHAANIEQCSMCAVLSPPPQPSSNQTLVDTEAIMATLTIGSLQIDSSSDPSPSVS EETPGYTNGHNEKSNCRKVPILTELKNPSNIHFIEQLGGLEGSLQETNLHLSTAFSTGTVFSGSFLDSLL ATAFYNYHVLELLQMLVTGGVSSQLEQHLDKDKVYGVADSCTSLLSGRNRCKLGLLSLHETILSDVNPRN TFGQLFCGSLDLFGILCVGLYRIIDEEELNPENKRFVITRPANEFKLLPSDLVFCAIPFSTACYKRNEEF SLQKSYEIVNKASQTTETHSDTNCPPTIDSVTETLYSPVYSYQPRTNSLSFPKQIAWNQSRTNSIISSQI PLGDNAKENERKTSDEVYDEDPFAYSEPL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001031836 |
| ORF Size | 3447 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001031836.3 |
| RefSeq Size | 3756 bp |
| RefSeq ORF | 3450 bp |
| Locus ID | 157855 |
| UniProt ID | A8MYU2 |
| Protein Families | Ion Channels: Potassium |
| Gene Summary | This gene encodes a member of the potassium channel family of proteins. The encoded voltage-gated ion channel allows the outward flow of potassium ions during plasma membrane hyperpolarization in sperm. Opening of this channel may be regulated by calcium ion levels. Homozygous knockout mice that lack the related mouse gene exhibit male sterility. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221094 | KCNU1 (Myc-DDK-tagged)-Human potassium channel, subfamily U, member 1 (KCNU1) |
CNY 8,000.00 |
|
| RC221094L3 | Lenti ORF clone of Human potassium channel, subfamily U, member 1 (KCNU1), Myc-DDK-tagged |
CNY 13,400.00 |
|
| RC221094L4 | Lenti ORF clone of Human potassium channel, subfamily U, member 1 (KCNU1), mGFP tagged |
CNY 13,400.00 |
|
| SC318525 | KCNU1 (untagged)-Human potassium channel, subfamily U, member 1 (KCNU1) |
CNY 22,140.00 |
