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PPP2R2D (NM_001003656) Human Tagged ORF Clone
CAT＃: RG221048
- TrueORF®
PPP2R2D (tGFP-tagged) - Human protein phosphatase 2, regulatory subunit B, delta isoform (PPP2R2D), transcript variant 2
ORF Plasmid: DDK
"NM_001003656" in other vectors (6)
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CNY 4,000.00
CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MDS026 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG221048 representing NM_001003656
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGCTGACCGAAGTCATCACTGCAGCCGAGTTCCACCCGCACCAGTGCAACGTGTTCGTCTACA GCAGTAGCAAAGGGACCATCCGCCTGTGTGACATGCGCTCCTCGGCCCTGTGCGACAGACACTCCAAGTT TTTTGAAGAGCCTGAAGATCCCAGCAGTAGGTCCTTCTTCTCAGAAATAATTTCATCCATATCCGATGTA AAATTCAGTCATAGTGGGCGGTACATGATGACCAGAGACTACCTGTCGGTGAAGGTGTGGGACCTCAACA TGGAGAGCAGGCCGGTGGAGACCCACCAGGTCCACGAGTACCTGCGCAGCAAGCTCTGCTCTCTCTATGA GAACGACTGCATCTTTGACAAGTTTGAGTGTTGCTGGAACGGTTCGGATAGCGCCATCATGACCGGGTCC TATAACAACTTCTTCAGGATGTTTGATAGAGACACGCGGAGGGATGTGACCCTGGAGGCCTCGAGAGAGA GCAGCAAACCGCGCGCCAGCCTCAAACCCCGGAAGGTGTGTACGGGGGGTAAGCGGAGGAAAGACGAGAT CAGTGTGGACAGTCTGGACTTCAACAAGAAGATCCTGCACACAGCCTGGCACCCCGTGGACAATGTCATT GCCGTGGCTGCCACCAATAACTTGTACATATTCCAGGACAAAATCAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221048 representing NM_001003656
Red=Cloning site Green=Tags(s) MEELTEVITAAEFHPHQCNVFVYSSSKGTIRLCDMRSSALCDRHSKFFEEPEDPSSRSFFSEIISSISDV KFSHSGRYMMTRDYLSVKVWDLNMESRPVETHQVHEYLRSKLCSLYENDCIFDKFECCWNGSDSAIMTGS YNNFFRMFDRDTRRDVTLEASRESSKPRASLKPRKVCTGGKRRKDEISVDSLDFNKKILHTAWHPVDNVI AVAATNNLYIFQDKIN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001003656 |
| ORF Size | 678 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001003656.1, NP_001003656.1 |
| RefSeq Size | 2091 bp |
| RefSeq ORF | 680 bp |
| Locus ID | 55844 |
| Protein Families | Druggable Genome, Phosphatase |
| Protein Pathways | Tight junction |
| Gene Summary | B regulatory subunit of protein phosphatase 2A (PP2A) that plays a key role in cell cycle by controlling mitosis entry and exit. The activity of PP2A complexes containing PPP2R2D (PR55-delta) fluctuate during the cell cycle: the activity is high in interphase and low in mitosis. During mitosis, activity of PP2A is inhibited via interaction with phosphorylated ENSA and ARPP19 inhibitors. Within the PP2A complexes, the B regulatory subunits modulate substrate selectivity and catalytic activity, and also may direct the localization of the catalytic enzyme to a particular subcellular compartment (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221048 | PPP2R2D (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B, delta isoform (PPP2R2D), transcript variant 2 |
CNY 2,400.00 |
|
| RC221048L1 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B, delta isoform (PPP2R2D), transcript variant 2, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC221048L2 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B, delta isoform (PPP2R2D), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC221048L3 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B, delta isoform (PPP2R2D), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC221048L4 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B, delta isoform (PPP2R2D), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| SC113475 | PPP2R2D (untagged)-Human protein phosphatase 2, regulatory subunit B, delta isoform (PPP2R2D), transcript variant 2 |
CNY 2,400.00 |
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