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MAN2A2 (NM_006122) Human Tagged ORF Clone
CAT＃: RG220394
- TrueORF®
MAN2A2 (tGFP-tagged) - Human mannosidase, alpha, class 2A, member 2 (MAN2A2)
ORF Plasmid: DDK
"NM_006122" in other vectors (4)
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CNY 12,640.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MANA2X |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG220394 representing NM_006122
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCTGAAAAAGCAGGTGACAGTGTGTGGGGCTGCCATCTTCTGTGTGGCAGTCTTCTCGCTCTACC TCATGCTGGACCGAGTGCAACACGATCCCACCCGACACCAGAATGGTGGGAACTTCCCCCGGAGCCAAAT TTCTGTGCTGCAGAACCGCATTGAGCAGCTGGAGCAGCTTTTGGAGGAGAACCATGAGATTATCAGCCAT ATCAAGGACTCCGTGCTGGAGCTGACAGCCAACGCAGAGGGCCCGCCCGCCATGCTGCCCTACTACACGG TCAATGGCTCCTGGGTGGTGCCACCGGAGCCCCGGCCCAGCTTCTTCTCCATCTCCCCGCAGGACTGCCA GTTTGCTTTGGGGGGCCGGGGTCAGAAGCCAGAGCTGCAGATGCTCACTGTGTCGGAGGAGCTGCCGTTT GACAACGTGGATGGTGGTGTGTGGAGGCAAGGCTTCGACATCTCCTACGACCCGCACGACTGGGATGCTG AAGACCTGCAGGTGTTTGTGGTGCCCCACTCTCACAATGACCCAGGCTGGATCAAGACCTTTGACAAGTA CTACACAGAGCAGACCCAACACATCCTCAATAGCATGGTGTCTAAGCTGCAGGAGGACCCCCGGCGGCGC TTCCTCTGGGCAGAGGTCTCCTTCTTCGCCAAGTGGTGGGACAACATCAATGTCCAAAAGAGAGCGGCAG TCCGAAGGCTGGTGGGAAACGGGCAGCTGGAGATTGCGACAGGAGGCTGGGTGATGCCAGATGAGGCCAA TTCCCACTACTTTGCATTGATTGACCAGCTCATCGAAGGACACCAGTGGCTGGAGAGAAATCTTGGTGCA ACCCCCCGCTCTGGCTGGGCAGTGGACCCCTTTGGATACAGCTCCACCATGCCTTACCTGCTGCGCCGTG CCAACCTCACCAGCATGCTGATTCAGAGAGTGCACTATGCCATCAAGAAGCACTTTGCTGCCACCCACAG CCTAGAGTTCATGTGGAGGCAGACATGGGACTCGGACTCCAGCACAGACATCTTCTGTCACATGATGCCC TTCTACAGCTATGACGTCCCCCATACCTGTGGCCCAGATCCCAAGATCTGCTGCCAATTTGATTTCAAAC GCCTGCCTGGTGGGCGCATCAACTGCCCTTGGAAGGTGCCACCCCGGGCCATCACAGAGGCCAACGTGGC AGAGAGGGCAGCCCTGCTTCTGGACCAATACCGGAAGAAGTCCCAGCTGTTCCGAAGCAACGTCCTCCTG GTGCCTCTTGGAGATGACTTCCGATATGACAAGCCCCAGGAGTGGGATGCCCAGTTCTTCAACTACCAAC GGCTCTTTGACTTCTTCAACAGCAGGCCTAACCTCCATGTGCAGGCCCAGTTTGGCACTCTTTCTGACTA TTTTGATGCCCTGTACAAGAGGACAGGGGTGGAGCCAGGGGCCCGGCCTCCAGGGTTTCCTGTGCTGAGC GGGGATTTCTTCTCCTATGCGGACCGGGAGGATCATTACTGGACAGGCTATTACACTTCCCGGCCCTTCT ACAAGAGCTTAGACCGAGTCCTGGAAGCCCACCTGCGGGGGGCAGAGGTTCTGTACAGCCTGGCTGCAGC TCACGCTCGCCGCTCTGGTCTGGCTGGCCGGTACCCACTGTCTGATTTCACCCTCCTGACGGAAGCTCGG CGCACATTGGGGCTCTTCCAGCATCACGATGCCATCACTGGCACGGCCAAGGAGGCTGTGGTGGTGGACT ATGGGGTCAGGCTTCTGCGCTCCCTTGTCAACCTGAAGCAGGTCATCATTCATGCAGCCCACTATCTGGT GCTGGGGGACAAGGAGACCTACCACTTTGACCCTGAGGCGCCCTTCCTCCAAGTGGATGACACTCGCTTA AGTCACGACGCCCTCCCAGAGCGCACGGTGATCCAGCTGGATTCCTCGCCCAGGTTTGTGGTCCTATTCA ACCCACTGGAACAGGAGCGATTCAGCATGGTGTCCCTGCTGGTCAACTCTCCCCGCGTGCGTGTCCTTTC GGAGGAGGGTCAGCCCCTGGCCGTGCAGATCAGCGCACACTGGAGCTCTGCCACCGAGGCGGTCCCTGAC GTCTACCAGGTGTCTGTGCCTGTCCGCCTGCCAGCCCTGGGCCTGGGCGTGCTGCAGCTACAGCTGGGCC TGGATGGGCACCGCACGCTGCCCTCCTCTGTGCGCATCTACCTGCACGGCCGGCAGCTGTCCGTCAGCAG GCACGAAGCGTTTCCTCTCCGTGTCATTGACTCTGGCACCAGCGACTTCGCCCTCAGCAACCGCTACATG CAGGTCTGGTTCTCAGGCCTTACTGGGCTCCTCAAGAGCATCCGAAGGGTGGATGAGGAGCACGAGCAGC AGGTGGACATGCAGGTCCTTGTCTATGGCACCCGTACGTCCAAAGACAAGAGTGGAGCCTACCTCTTCCT GCCCGATGGCGAGGCCAAGCCCTACGTCCCCAAGGAGCCCCCCGTGCTGCGTGTCACTGAAGGCCCTTTC TTCTCAGAGGTGGTTGCGTACTATGAGCACATTCACCAGGCGGTCCGGCTTTACAATCTGCCAGGGGTGG AGGGGCTGTCTCTGGACATATCATCCCTGGTGGACATCCGGGACTACGTCAACAAGGAGCTGGCCCTGCA CATCCATACAGACATCGACAGCCAGGGTATCTTCTTCACAGACCTCAATGGCTTTCAGGTGCAGCCCCGA CGGTATCTGAAGAAGCTCCCCCTCCAGGCCAACTTCTACCCCATGCCAGTCATGGCCTATATCCAGGACG CACAGAAGCGCCTCACGCTGCACACTGCCCAGGCCCTGGGTGTCTCTAGCCTCAAAGATGGCCAGCTGGA GGTGATCTTGGACCGGCGGCTGATGCAGGATGACAACCGGGGCCTAGGCCAAGGGCTCAAGGACAACAAG AGAACCTGCAACCGTTTCCGCCTCCTGCTAGAGCGGCGAACCGTGGGCAGTGAGGTCCAAGATAGCCACT CTACCAGCTACCCATCCCTCCTCAGCCACCTGACCTCCATGTACCTGAACGCCCCGGCGCTCGCTCTGCC TGTAGCCAGGATGCAGCTCCCAGGCCCTGGTCTGCGCTCATTTCATCCTCTGGCTTCCTCACTGCCCTGT GACTTCCACCTGCTCAACCTACGTACGCTCCAGGCTGAGGAGGACACCCTACCCTCGGCGGAGACCGCAC TCATCTTACACCGCAAGGGTTTTGACTGCGGCCTGGAGGCCAAGAACTTGGGCTTCAACTGCACCACAAG CCAAGGCAAGGTAGCCCTGGGCAGCCTTTTCCATGGCCTGGATGTGGTATTCCTTCAGCCAACCTCCTTG ACGTTACTGTACCCTCTGGCCTCCCCGTCCAACAGCACTGACGTCTATTTGGAGCCCATGGAGATTGCTA CCTTTCGCCTCCGCTTGGGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG220394 representing NM_006122
Red=Cloning site Green=Tags(s) MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPTRHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISH IKDSVLELTANAEGPPAMLPYYTVNGSWVVPPEPRPSFFSISPQDCQFALGGRGQKPELQMLTVSEELPF DNVDGGVWRQGFDISYDPHDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRR FLWAEVSFFAKWWDNINVQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLERNLGA TPRSGWAVDPFGYSSTMPYLLRRANLTSMLIQRVHYAIKKHFAATHSLEFMWRQTWDSDSSTDIFCHMMP FYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVAERAALLLDQYRKKSQLFRSNVLL VPLGDDFRYDKPQEWDAQFFNYQRLFDFFNSRPNLHVQAQFGTLSDYFDALYKRTGVEPGARPPGFPVLS GDFFSYADREDHYWTGYYTSRPFYKSLDRVLEAHLRGAEVLYSLAAAHARRSGLAGRYPLSDFTLLTEAR RTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVNLKQVIIHAAHYLVLGDKETYHFDPEAPFLQVDDTRL SHDALPERTVIQLDSSPRFVVLFNPLEQERFSMVSLLVNSPRVRVLSEEGQPLAVQISAHWSSATEAVPD VYQVSVPVRLPALGLGVLQLQLGLDGHRTLPSSVRIYLHGRQLSVSRHEAFPLRVIDSGTSDFALSNRYM QVWFSGLTGLLKSIRRVDEEHEQQVDMQVLVYGTRTSKDKSGAYLFLPDGEAKPYVPKEPPVLRVTEGPF FSEVVAYYEHIHQAVRLYNLPGVEGLSLDISSLVDIRDYVNKELALHIHTDIDSQGIFFTDLNGFQVQPR RYLKKLPLQANFYPMPVMAYIQDAQKRLTLHTAQALGVSSLKDGQLEVILDRRLMQDDNRGLGQGLKDNK RTCNRFRLLLERRTVGSEVQDSHSTSYPSLLSHLTSMYLNAPALALPVARMQLPGPGLRSFHPLASSLPC DFHLLNLRTLQAEEDTLPSAETALILHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSL TLLYPLASPSNSTDVYLEPMEIATFRLRLG TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006122 |
| ORF Size | 3450 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006122.4 |
| RefSeq Size | 6279 bp |
| RefSeq ORF | 3453 bp |
| Locus ID | 4122 |
| UniProt ID | P49641 |
| Domains | Glyco_hydro_38 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Metabolic pathways, N-Glycan biosynthesis |
| Gene Summary | Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Homozygous truncating variant in MAN2A2 causes a novel congenital disorder of glycosylation with neurological involvement.
,null,
Journal of medical genetics
,PubMed ID 36357165
[MAN2A2]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220394 | MAN2A2 (Myc-DDK-tagged)-Human mannosidase, alpha, class 2A, member 2 (MAN2A2) |
CNY 8,008.00 |
|
| RC220394L3 | Lenti-ORF clone of MAN2A2 (Myc-DDK-tagged)-Human mannosidase, alpha, class 2A, member 2 (MAN2A2) |
CNY 13,400.00 |
|
| RC220394L4 | Lenti-ORF clone of MAN2A2 (mGFP-tagged)-Human mannosidase, alpha, class 2A, member 2 (MAN2A2) |
CNY 13,400.00 |
|
| SC315227 | MAN2A2 (untagged)-Human mannosidase, alpha, class 2A, member 2 (MAN2A2) |
CNY 10,450.00 |
