P2Y10 (P2RY10) (NM_198333) Human Tagged ORF Clone

CAT#: RG220149

  • TrueORF®

P2RY10 (tGFP-tagged) - Human purinergic receptor P2Y, G-protein coupled, 10 (P2RY10), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_198333" in other vectors (4)

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CNY 4,370.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


P2Y10 / P2RY10 Rabbit Polyclonal (Extracellular Domain) Antibody
    • 50 ug

CNY 5,654.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms LYPSR2; P2Y10
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG220149 representing NM_198333
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTAACCTTGACAAATACACTGAAACATTCAAGATGGGTAGCAACAGTACCAGCACTGCTGAGATTT
ACTGTAATGTCACTAATGTGAAATTTCAATACTCCCTCTATGCAACCACCTATATCCTCATATTCATTCC
TGGTCTTCTGGCTAACAGTGCAGCCTTGTGGGTTCTGTGCCGCTTCATCAGCAAGAAAAATAAAGCCATC
ATTTTCATGATCAACCTCTCTGTGGCTGACCTTGCTCATGTATTATCTTTACCCCTCCGGATTTACTATT
ACATCAGCCACCACTGGCCTTTCCAGAGAGCCCTTTGCCTGCTCTGCTTCTACCTGAAGTATCTCAACAT
GTATGCCAGCATTTGTTTCCTGACGTGCATCAGTCTTCAAAGGTGCTTTTTTCTCCTCAAGCCCTTCAGG
GCCAGAGACTGGAAGCGTAGGTACGATGTGGGCATCAGTGCTGCCATCTGGATCGTTGTGGGGACTGCAT
GTTTGCCATTTCCCATCCTGAGAAGCACAGACTTAAACAACAACAAGTCCTGCTTTGCTGATCTTGGATA
CAAGCAAATGAATGCAGTTGCGTTGGTCGGGATGATTACAGTTGCTGAGCTTGCAGGATTTGTGATCCCA
GTGATCATCATCGCATGGTGTACCTGGAAAACTACTATATCCTTGAGACAGCCACCAATGGCTTTCCAAG
GGATCAGTGAGAGGCAGAAAGCACTGCGGATGGTGTTCATGTGTGCTGCAGTCTTCTTCATCTGCTTCAC
TCCCTATCATATTAACTTTATTTTTTACACCATGGTAAAGGAAACCATCATTAGCAGTTGTCCCGTTGTC
CGAATCGCACTGTATTTCCACCCTTTTTGCCTGTGCCTTGCAAGTCTCTGCTGCCTTTTGGATCCAATTC
TTTATTACTTTATGGCTTCAGAGTTTCGTGACCAACTATCCCGCCATGGCAGTTCTGTGACCCGCTCCCG
CCTCATGAGCAAGGAGAGTGGTTCATCAATGATTGGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG220149 representing NM_198333
Red=Cloning site Green=Tags(s)

MANLDKYTETFKMGSNSTSTAEIYCNVTNVKFQYSLYATTYILIFIPGLLANSAALWVLCRFISKKNKAI
IFMINLSVADLAHVLSLPLRIYYYISHHWPFQRALCLLCFYLKYLNMYASICFLTCISLQRCFFLLKPFR
ARDWKRRYDVGISAAIWIVVGTACLPFPILRSTDLNNNKSCFADLGYKQMNAVALVGMITVAELAGFVIP
VIIIAWCTWKTTISLRQPPMAFQGISERQKALRMVFMCAAVFFICFTPYHINFIFYTMVKETIISSCPVV
RIALYFHPFCLCLASLCCLLDPILYYFMASEFRDQLSRHGSSVTRSRLMSKESGSSMIG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_198333
ORF Size 1017 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_198333.1, NP_938147.1
RefSeq Size 1679 bp
RefSeq ORF 1020 bp
Locus ID 27334
UniProt ID O00398
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
Gene Summary The protein encoded by this gene belongs to the family of G-protein coupled receptors that are preferentially activated by adenosine and uridine nucleotides. There is a pseudogene for this gene nearby on chromosome X. Multiple alternatively spliced transcripts have been observed. [provided by RefSeq, Apr 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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