United States
Germany
Japan
United Kingdom
China
Amphiphysin (AMPH) (NM_139316) Human Tagged ORF Clone
CAT＃: RG220048
- TrueORF®
AMPH (tGFP-tagged) - Human amphiphysin (AMPH), transcript variant 2
ORF Plasmid: DDK
"NM_139316" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AMPH1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG220048 representing NM_139316
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGACATCAAGACGGGCATCTTCGCCAAGAACGTCCAGAAGCGACTCAACCGCGCGCAGGAAAAGG TCCTCCAAAAGCTGGGGAAAGCTGATGAGACAAAAGACGAACAGTTCGAAGAATATGTCCAGAACTTCAA ACGGCAAGAAGCAGAGGGTACCAGACTTCAGCGAGAACTCCGAGGATATTTAGCAGCAATCAAAGGCATG CAGGAGGCCTCCATGAAGCTCACAGAGTCGCTGCATGAAGTCTATGAGCCTGACTGGTATGGGCGGGAAG ATGTGAAAATGGTTGGTGAGAAATGTGATGTGCTGTGGGAAGACTTCCATCAAAAACTCGTGGATGGGTC CTTGCTAACACTGGATACCTACCTGGGGCAATTTCCTGACATAAAGAATCGCATCGCCAAGCGCAGCAGG AAGCTAGTGGACTATGACAGTGCCCGCCACCATCTGGAAGCTCTGCAGAGCTCCAAGAGGAAGGATGAGA GTCGAATCTCTAAGGCAGAAGAAGAATTTCAGAAAGCACAGAAAGTGTTTGAAGAGTTTAACGTTGACTT ACAAGAAGAGTTACCATCATTATGGTCAAGACGAGTTGGATTTTATGTTAATACTTTCAAAAACGTCTCC AGCCTTGAAGCCAAGTTTCATAAGGAAATTGCGGTGCTTTGCCACAAACTGTATGAAGTGATGACAAAAC TGGGTGACCAGCACGCCGACAAGGCCTTCACCATCCAAGGAGCGCCCAGTGATTCGGGTCCTCTCCGCAT TGCAAAGACACCATCACCGCCTGAGGAGCCTTCACCCCTCCCGAGCCCGACAGCAAGTCCAAATCATACA TTAGCACCTGCGTCTCCCGCACCAGCACGGCCTCGGTCACCTTCACAGACAAGGAAAGGGCCTCCTGTCC CACCTCTACCTAAAGTCACCCCGACAAAGGAACTGCAGCAGGAGAACATCATCAGTTTCTTTGAGGACAA CTTTGTTCCAGAAATCAGTGTGACAACACCTTCCCAGAATGAAGTCCCTGAGGTGAAGAAAGAGGAGACT TTGCTGGATCTGGACTTTGATCCTTTCAAGCCCGAGGTGACACCTGCAGGTTCTGCTGGAGTGACCCACT CACCCATGTCTCAGACATTGCCCTGGGACCTATGGACGACAAGCACTGATTTGGTACAGCCGGCTTCTGG TGGTTCATTTAATGGATTCACACAGCCCCAGGATACTTCATTATTCACAATGCAGACAGACCAGAGTATG ATCTGCAACTTGATCATACCTGGAGCTGATGCTGATGCAGCTGTTGGAACCTTGGTGTCAGCAGCTGAGG GGGCCCCAGGAGAGGAAGCAGAGGCGGAGAAGGCCACTGTCCCTGCCGGGGAAGGAGTAAGTTTAGAGGA GGCCAAAATTGGAACTGAAACCACTGAGGGTGCAGAGAGTGCCCAACCTGAAGCAGAGGAGCTCGAAGCA ACAGTGCCTCAGGAGAAGGTCATTCCTTCGGTGGTCATAGAGCCTGCCTCCAACCATGAAGAGGAAGGAG AAAACGAAATAACTATAGGTGCAGAGCCCAAGGAGACCACCGAGGACGCGGCTCCTCCGGGCCCCACCAG CGAGACACCGGAGCTGGCTACGGAGCAGAAGCCTATCCAGGACCCTCAGCCCACGCCTTCTGCACCAGCC ATGGGGGCTGCTGACCAGCTAGCATCTGCAAGGGAGGCCTCTCAGGAATTGCCTCCTGGCTTTCTCTACA AGGTGGAAACACTGCATGATTTTGAGGCAGCAAATTCTGATGAACTTACCTTACAAAGGGGTGATGTGGT GCTGGTGGTCCCCTCAGATTCAGAAGCTGATCAGGATGCAGGCTGGCTGGTGGGAGTGAAGGAATCAGAC TGGCTTCAGTACAGAGACCTTGCCACCTACAAAGGCCTCTTTCCAGAGAACTTCACCCGACGCTTAGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG220048 representing NM_139316
Red=Cloning site Green=Tags(s) MADIKTGIFAKNVQKRLNRAQEKVLQKLGKADETKDEQFEEYVQNFKRQEAEGTRLQRELRGYLAAIKGM QEASMKLTESLHEVYEPDWYGREDVKMVGEKCDVLWEDFHQKLVDGSLLTLDTYLGQFPDIKNRIAKRSR KLVDYDSARHHLEALQSSKRKDESRISKAEEEFQKAQKVFEEFNVDLQEELPSLWSRRVGFYVNTFKNVS SLEAKFHKEIAVLCHKLYEVMTKLGDQHADKAFTIQGAPSDSGPLRIAKTPSPPEEPSPLPSPTASPNHT LAPASPAPARPRSPSQTRKGPPVPPLPKVTPTKELQQENIISFFEDNFVPEISVTTPSQNEVPEVKKEET LLDLDFDPFKPEVTPAGSAGVTHSPMSQTLPWDLWTTSTDLVQPASGGSFNGFTQPQDTSLFTMQTDQSM ICNLIIPGADADAAVGTLVSAAEGAPGEEAEAEKATVPAGEGVSLEEAKIGTETTEGAESAQPEAEELEA TVPQEKVIPSVVIEPASNHEEEGENEITIGAEPKETTEDAAPPGPTSETPELATEQKPIQDPQPTPSAPA MGAADQLASAREASQELPPGFLYKVETLHDFEAANSDELTLQRGDVVLVVPSDSEADQDAGWLVGVKESD WLQYRDLATYKGLFPENFTRRLD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_139316 |
| ORF Size | 1959 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_139316.2, NP_647477.1 |
| RefSeq Size | 3161 bp |
| RefSeq ORF | 1962 bp |
| Locus ID | 273 |
| UniProt ID | P49418 |
| Domains | SH3, BAR |
| Protein Families | Secreted Protein |
| Protein Pathways | Fc gamma R-mediated phagocytosis |
| Gene Summary | This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Nov 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220048 | AMPH (Myc-DDK-tagged)-Human amphiphysin (AMPH), transcript variant 2 |
CNY 4,560.00 |
|
| RC220048L3 | Lenti-ORF clone of AMPH (Myc-DDK-tagged)-Human amphiphysin (AMPH), transcript variant 2 |
CNY 6,460.00 |
|
| RC220048L4 | Lenti-ORF clone of AMPH (mGFP-tagged)-Human amphiphysin (AMPH), transcript variant 2 |
CNY 6,460.00 |
|
| SC310194 | AMPH (untagged)-Human amphiphysin (AMPH), transcript variant 2 |
CNY 10,450.00 |
其它Amphiphysin产品
