EIF4E1B (NM_001099408) Human Tagged ORF Clone
CAT#: RG219823
- TrueORF®
EIF4E1B (tGFP-tagged) - Human eukaryotic translation initiation factor 4E family member 1B (EIF4E1B)
ORF Plasmid: DDK
"NM_001099408" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG219823 representing NM_001099408
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTGCTGTTGAGGTGAGTGAAGCTGAGGGTGGAATCCGAGAGTGGGAGGAGGAGGAGAAGGAGGAGG AGGCAGCAGAGAGGACGCCCACAGGAGAAAAGTCTCCAAACTCTCCCAGGACTTTGCTGTCTCTGAGAGG GAAGGCCCGGACGGGGGGCCCCATGGAAGTCAAGCTGGAGCTGCACCCCTTGCAGAACAGGTGGGCTCTG TGGTTCTTCAAGAATGACCGCAGCCGGGCCTGGCAGGACAACCTGCACCTGGTCACCAAGGTGGACACTG TGGAGGACTTCTGGGCGCTATACAGTCACATCCAGCTGGCCAGCAAGCTCTCCTCTGGCTGTGACTACGC CCTCTTCAAGGATGGCATCCAGCCCATGTGGGAGGACAGCAGGAATAAACGGGGTGGCCGCTGGCTGGTC AGCCTGGCCAAGCAGCAGCGCCACATTGAGCTGGACCGGCTGTGGCTGGAGACGCTGCTGTGTCTGATCG GGGAGAGCTTTGAGGAACACAGCAGAGAGGTATGTGGGGCCGTCGTCAACATCCGCACCAAGGGGGACAA GATCGCTGTGTGGACGAGGGAGGCGGAAAACCAGGCGGGCGTGCTGCACGTTGGGCGTGTATACAAAGAG CGCCTGGGCCTCTCCCCAAAGACCATCATTGGGTACCAGGCCCATGCAGACACAGCCACCAAGAGCAACT CCCTAGCCAAGAACAAGTTTGTGGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG219823 representing NM_001099408
Red=Cloning site Green=Tags(s) MLAVEVSEAEGGIREWEEEEKEEEAAERTPTGEKSPNSPRTLLSLRGKARTGGPMEVKLELHPLQNRWAL WFFKNDRSRAWQDNLHLVTKVDTVEDFWALYSHIQLASKLSSGCDYALFKDGIQPMWEDSRNKRGGRWLV SLAKQQRHIELDRLWLETLLCLIGESFEEHSREVCGAVVNIRTKGDKIAVWTREAENQAGVLHVGRVYKE RLGLSPKTIIGYQAHADTATKSNSLAKNKFVV TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001099408 |
ORF Size | 726 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001099408.2 |
RefSeq Size | 1974 bp |
RefSeq ORF | 729 bp |
Locus ID | 253314 |
UniProt ID | A6NMX2 |
Protein Pathways | Insulin signaling pathway, mTOR signaling pathway |
Gene Summary | Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structure.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC219823 | EIF4E1B (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4E family member 1B (EIF4E1B) |
CNY 2,400.00 |
|
RC219823L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 4E family member 1B (EIF4E1B), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC219823L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 4E family member 1B (EIF4E1B), mGFP tagged |
CNY 5,890.00 |
|
SC316492 | EIF4E1B (untagged)-Human eukaryotic translation initiation factor 4E family member 1B (EIF4E1B) |
CNY 3,990.00 |
其它EIF4E1B产品