United States
Germany
Japan
United Kingdom
China
SART2 (DSE) (NM_001080976) Human Tagged ORF Clone
CAT＃: RG218893
- TrueORF®
DSE (tGFP-tagged) - Human dermatan sulfate epimerase (DSE), transcript variant 2
ORF Plasmid: DDK
"NM_001080976" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DS-epi1; DSEP; DSEPI; EDSMC2; SART-2; SART2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG218893 representing NM_001080976
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGACTCACACACGGGGGGCTCCCAGTGTGTTTTTCATATATTTGCTTTGCTTTGTGTCAGCCTACA TCACCGACGAGAACCCAGAAGTTATGATTCCCTTCACCAATGCCAACTACGACAGCCATCCCATGCTGTA CTTCTCCAGGGCAGAAGTGGCGGAGCTGCAGCTCAGGGCTGCCAGCTCGCACGAGCACATTGCAGCCCGC CTCACGGAGGCTGTGCACACGATGCTGTCCAGCCCCTTGGAATACCTCCCTCCCTGGGATCCCAAGGACT ACAGTGCCCGCTGGAATGAAATTTTTGGAAACAACTTGGGTGCCTTGGCAATGTTCTGTGTGCTGTATCC TGAGAACATTGAAGCCCGAGACATGGCCAAAGACTACATGGAGAGGATGGCAGCGCAGCCTAGTTGGTTG GTGAAAGATGCTCCTTGGGATGAGGTCCCGCTTGCTCACTCCCTGGTTGGTTTTGCCACTGCTTATGACT TCTTGTACAACTACCTGAGCAAGACACAACAGGAGAAGTTTCTTGAAGTGATTGCCAATGCCTCAGGGTA TATGTATGAAACTTCATACAGGAGAGGATGGGGATTTCAATACCTGCACAATCATCAGCCCACCAACTGT ATGGCTTTGCTCACGGGAAGCCTAGTCCTGATGAATCAAGGATATCTTCAAGAAGCCTACTTATGGACCA AACAAGTTCTGACCATCATGGAGAAATCTCTGGTCTTGCTCAGGGAGGTGACGGATGGCTCCCTCTATGA AGGAGTTGCGTATGGCAGCTACACCACTAGATCACTCTTCCAATACATGTTTCTCGTCCAGAGGCACTTC AACATCAACCACTTTGGCCATCCGTGGCTTAAACAACACTTTGCATTTATGTATAGAACCATCCTGCCAG GGTTTCAAAGGACTGTGGCTATTGCGGACTCAAATTACAACTGGTTTTATGGTCCAGAAAGCCAATTAGT GTTCCTTGATAAATTTGTCATGCGTAATGGCAGTGGTAACTGGCTAGCTGACCAAATCAGAAGGAACCGT GTGGTGGAAGGTCCAGGAACACCATCCAAAGGGCAGCGCTGGTGCACTCTGCACACAGAATTTCTCTGGT ATGATGGCAGCTTGAAATCGGTTCCTCCTCCAGACTTTGGCACCCCTACACTGCATTATTTTGAAGACTG GGGTGTCGTGACTTATGGAAGTGCACTACCTGCAGAAATCAATAGATCTTTCCTTTCCTTCAAGTCTGGA AAACTGGGGGGACGTGCAATATATGACATTGTCCACAGAAACAAATACAAAGATTGGATCAAAGGATGGA GAAATTTTAATGCAGGGCATGAACATCCTGATCAAAACTCATTTACTTTTGCTCCCAATGGTGTGCCTTT CATTACTGAGGCTCTGTACGGGCCAAAGTACACCTTCTTCAACAATGTTTTGATGTTTTCCCCAGCTGTG TCAAAGAGCTGCTTTTCTCCCTGGGTGGGTCAGGTCACAGAAGACTGCTCATCAAAATGGTCTAAATACA AGCATGACCTGGCAGCTAGTTGTCAGGGGAGGGTGGTTGCAGCAGAGGAGAAAAATGGGGTGGTTTTCAT CCGAGGAGAAGGTGTGGGAGCTTATAACCCCCAGCTCAACCTGAAGAATGTTCAGAGGAATCTCATCCTC CTACATCCACAGCTGCTTCTCCTTGTAGACCAAATACACCTGGGAGAGGAGAGTCCCTTGGAGACAGCAG CGAGCTTCTTCCATAATGTGGATGTTCCTTTTGAGGAGACTGTGGTAGATGGTGTCCATGGGGCTTTCAT CAGGCAGAGAGATGGTCTCTATAAAATGTACTGGATGGACGATACTGGCTACAGCGAGAAAGCAACCTTT GCCTCAGTGACATATCCTCGGGGCTATCCCTACAACGGGACAAACTATGTGAATGTCACCATGCACCTCC GAAGTCCCATCACCAGGGCAGCTTACCTCTTCATAGGGCCATCTATAGATGTTCAGAGCTTCACTGTCCA CGGAGACTCTCAGCAACTGGATGTGTTCATAGCCACCAGCAAACATGCCTACGCCACATACCTGTGGACA GGTGAGGCCACAGGACAGTCTGCCTTTGCACAGGTCATTGCTGATCGTCACAAAATTCTGTTTGACCGGA ATTCAGCCATCAAGAGCAGCATTGTCCCTGAGGTGAAGGACTATGCTGCTATTGTGGAACAGAACTTGCA GCATTTTAAACCAGTGTTTCAGCTGCTGGAGAAGCAGATACTGTCCCGAGTCCGGAACACAGCTAGCTTT AGGAAGACTGCTGAACGCCTGCTGAGATTTTCAGATAAGAGACAGACTGAGGAGGCCATTGACAGGATTT TTGCCATATCACAGCAACAGCAGCAGCAAAGCAAGTCAAAGAAAAACCGAAGGGCAGGCAAACGCTATAA ATTTGTGGATGCTGTCCCTGATATTTTTGCACAGATTGAAGTCAATGAGAAAAAGATTAGACAGAAAGCT CAGATTTTGGCACAGAAAGAACTACCCATAGATGAAGATGAAGAAATGAAAGACCTTTTAGATTTTGCAG ATGTAACATACGAGAAACATAAAAATGGGGGCTTGATTAAAGGCCGGTTTGGACAGGCACGGATGGTGAC AACTACACACAGCAGGGCCCCATCACTGTCTGCTTCCTATACCAGGTTGTTCCTGATTCTGAACATTGCT ATTTTCTTTGTCATGTTGGCAATGCAACTGACTTATTTCCAGAGGGCCCAGAGCCTACATGGCCAAAGAT GTCTTTATGCAGTTCTTCTCATAGATAGCTGTATTTTATTATGGTTGTACTCTTCTTGTTCCCAATCACA GTGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG218893 representing NM_001080976
Red=Cloning site Green=Tags(s) MRTHTRGAPSVFFIYLLCFVSAYITDENPEVMIPFTNANYDSHPMLYFSRAEVAELQLRAASSHEHIAAR LTEAVHTMLSSPLEYLPPWDPKDYSARWNEIFGNNLGALAMFCVLYPENIEARDMAKDYMERMAAQPSWL VKDAPWDEVPLAHSLVGFATAYDFLYNYLSKTQQEKFLEVIANASGYMYETSYRRGWGFQYLHNHQPTNC MALLTGSLVLMNQGYLQEAYLWTKQVLTIMEKSLVLLREVTDGSLYEGVAYGSYTTRSLFQYMFLVQRHF NINHFGHPWLKQHFAFMYRTILPGFQRTVAIADSNYNWFYGPESQLVFLDKFVMRNGSGNWLADQIRRNR VVEGPGTPSKGQRWCTLHTEFLWYDGSLKSVPPPDFGTPTLHYFEDWGVVTYGSALPAEINRSFLSFKSG KLGGRAIYDIVHRNKYKDWIKGWRNFNAGHEHPDQNSFTFAPNGVPFITEALYGPKYTFFNNVLMFSPAV SKSCFSPWVGQVTEDCSSKWSKYKHDLAASCQGRVVAAEEKNGVVFIRGEGVGAYNPQLNLKNVQRNLIL LHPQLLLLVDQIHLGEESPLETAASFFHNVDVPFEETVVDGVHGAFIRQRDGLYKMYWMDDTGYSEKATF ASVTYPRGYPYNGTNYVNVTMHLRSPITRAAYLFIGPSIDVQSFTVHGDSQQLDVFIATSKHAYATYLWT GEATGQSAFAQVIADRHKILFDRNSAIKSSIVPEVKDYAAIVEQNLQHFKPVFQLLEKQILSRVRNTASF RKTAERLLRFSDKRQTEEAIDRIFAISQQQQQQSKSKKNRRAGKRYKFVDAVPDIFAQIEVNEKKIRQKA QILAQKELPIDEDEEMKDLLDFADVTYEKHKNGGLIKGRFGQARMVTTTHSRAPSLSASYTRLFLILNIA IFFVMLAMQLTYFQRAQSLHGQRCLYAVLLIDSCILLWLYSSCSQSQC TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001080976 |
| ORF Size | 2874 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001080976.2, NP_001074445.1 |
| RefSeq Size | 4043 bp |
| RefSeq ORF | 2877 bp |
| Locus ID | 29940 |
| UniProt ID | Q9UL01 |
| Protein Families | Transmembrane |
| Protein Pathways | Chondroitin sulfate biosynthesis |
| Gene Summary | The protein encoded by this gene is a tumor-rejection antigen. It is localized to the endoplasmic reticulum and functions to convert D-glucuronic acid to L-iduronic acid during the biosynthesis of dermatan sulfate. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. Mutations in this gene cause inmusculocontractural Ehlers-Danlos syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 9, and a paralogous gene exists on chromosome 18. [provided by RefSeq, Apr 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218893 | DSE (Myc-DDK-tagged)-Human dermatan sulfate epimerase (DSE), transcript variant 2 |
CNY 3,990.00 |
|
| RC218893L3 | Lenti ORF clone of Human dermatan sulfate epimerase (DSE), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC218893L4 | Lenti ORF clone of Human dermatan sulfate epimerase (DSE), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| SC315973 | DSE (untagged)-Human dermatan sulfate epimerase (DSE), transcript variant 2 |
CNY 7,220.00 |
