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HIPK3 (NM_001048200) Human Tagged ORF Clone
CAT＃: RG218848
- TrueORF®
HIPK3 (tGFP-tagged) - Human homeodomain interacting protein kinase 3 (HIPK3), transcript variant 3
ORF Plasmid: DDK
"NM_001048200" in other vectors (4)
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CNY 13,110.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DYRK6; FIST3; PKY; YAK1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG218848 representing NM_001048200
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCACAAGTCTTGGTCTACCCACCATATGTTTATCAAACTCAGTCAAGTGCCTTTTGTAGTGTGA AGAAACTCAAAGTAGAGCCAAGCAGTTGTGTATTCCAGGAAAGAAACTATCCACGGACCTATGTGAATGG TAGAAACTTTGGAAATTCTCATCCTCCCACTAAGGGTAGTGCTTTTCAGACAAAGATACCATTTAATAGA CCTCGAGGACACAACTTTTCATTGCAGACAAGTGCTGTTGTTTTGAAAAACACTGCAGGTGCTACAAAGG TCATAGCAGCTCAGGCACAGCAAGCTCACGTGCAGGCACCTCAGATTGGGGCGTGGCGAAACAGATTGCA TTTCCTAGAAGGCCCCCAGCGATGTGGATTGAAGCGCAAGAGTGAGGAGTTGGATAATCATAGCAGCGCA ATGCAGATTGTCGATGAATTGTCCATACTTCCTGCAATGTTGCAAACCAACATGGGAAATCCAGTGACAG TTGTGACAGCTACCACAGGATCAAAACAGAATTGTACCACTGGAGAAGGTGACTATCAGTTAGTACAGCA TGAAGTCTTATGCTCCATGAAAAATACTTACGAAGTCCTTGATTTTCTTGGTCGAGGCACGTTTGGCCAG GTAGTTAAATGCTGGAAAAGAGGGACAAATGAAATTGTAGCAATCAAAATTTTGAAGAATCATCCTTCTT ATGCCCGTCAAGGTCAAATAGAAGTGAGCATATTAGCAAGGCTCAGTACTGAAAATGCTGATGAATATAA CTTTGTACGAGCTTATGAATGCTTTCAGCACCGTAACCATACTTGTTTAGTCTTTGAGATGCTGGAACAA AACTTGTATGACTTTCTGAAACAAAATAAATTTAGTCCCCTGCCACTAAAAGTGATTCGGCCCATTCTTC AACAAGTGGCCACTGCACTGAAAAAATTGAAAAGTCTTGGTTTAATTCATGCTGATCTCAAGCCAGAGAA TATTATGTTGGTGGATCCTGTTCGGCAGCCTTACAGGGTTAAAGTAATAGACTTTGGGTCGGCCAGTCAT GTATCAAAGACTGTTTGTTCAACATATCTACAATCTCGGTACTACAGAGCTCCAGAGATTATATTGGGGT TGCCATTTTGTGAAGCCATAGACATGTGGTCATTGGGATGTGTGATTGCAGAATTATTTCTTGGATGGCC GCTCTACCCAGGAGCCTTGGAGTATGATCAGATTCGATACATTTCTCAGACTCAAGGTTTGCCAGGAGAA CAGTTGTTAAATGTGGGTACTAAATCCACAAGATTTTTTTGCAAAGAAACAGATATGTCTCATTCTGGTT GGAGATTAAAGACATTGGAAGAGCATGAGGCAGAGACAGGAATGAAGTCTAAAGAAGCCAGAAAATACAT TTTCAACAGTCTGGATGATGTAGCGCATGTGAACACAGTGATGGATTTGGAAGGAAGTGATCTTTTGGCT GAGAAAGCTGATAGAAGAGAATTTGTTAGTCTGTTGAAGAAAATGTTGCTGATTGATGCAGATTTAAGAA TTACTCCAGCTGAGACCCTGAACCATCCTTTTGTTAATATGAAACATCTTCTAGATTTCCCTCATAGCAA CCATGTAAAGTCCTGTTTTCATATTATGGATATTTGTAAGTCCCACCTAAATTCATGTGACACAAATAAT CACAACAAAACTTCACTTTTAAGACCAGTTGCTTCAAGCAGTACTGCTACACTGACTGCAAATTTTACTA AAATCGGAACATTAAGAAGTCAGGCATTGACCACATCTGCTCATTCAGTTGTGCACCATGGAATACCTCT GCAGGCAGGAACTGCTCAGTTTGGTTGTGGTGATGCTTTTCAGCAGACATTGATTATCTGTCCCCCAGCT ATTCAAGGTATTCCTGCAACACATGGTAAACCCACCAGTTATTCAATAAGGGTAGATAATACAGTTCCAC TTGTAACTCAGGCCCCAGCTGTGCAGCCACTACAGATCCGACCAGGAGTTCTTTCTCAGACGTGGTCTGG TAGAACACAGCAGATGCTGGTGCCTGCCTGGCAACAGGTGACACCCCTGGCTCCTGCTACTACTACACTA ACTTCTGAGAGTGTGGCTGGTTCACACAGGCTTGGAGACTGGGGGAAGATGATTTCATGCAGCAATCATT ATAACTCAGTGATGCCGCAGCCTCTTCTGACCAATCAGATAACTTTATCTGCCCCTCAGCCAGTTAGTGT GGGGATTGCACATGTTGTCTGGCCTCAGCCTGCCACTACCAAGAAAAATAAACAGTGCCAGAACAGGAGT AATTCATTACAGAATACCAATATCCCACATTCAGCATTTATTTCTCCAAAGATAATTAATGGGAAAGATG TCGAGGAAGTAAGTTGTATAGAAACACAGGACAATCAGAACTCAGAAGGAGAGGCAAGAAATTGCTGTGA AACATCTATCAGACAGGACTCTGATTCATCAGTTTCAGACAAACAGCGGCAAACCATCATTATTGCCGAC TCCCCGAGTCCTGCAGTGAGTGTCATCACTATCAGCAGTGACACTGATGAGGAAGAGACTTCCCAGAGAC ATTCACTCAGAGAATGTAAAGGTAGTCTAGATTGTGAAGCTTGCCAGAGCACTTTGAATATTGATCGGAT GTGTTCATTAAGTAGTCCTGATAGTACTCTGAGTACCAGCTCCTCAGGGCAGTCCAGCCCATCCCCCTGC AAGAGACCGAATAGTATGTCAGATGAAGAGCAAGAAAGTAGTTGTGATACGGTGGATGGCTCTCCGACAT CTGACTCTTCCGGGCATGACAGTCCATTTGCAGAGAGCACTTTTGTGGAGGACACTCATGAAAACACAGA ATTGGTATCCTCTGCTGACACAGAAACCAAGCCAGCTGTCTGTTCTGTTGTGGTGCCACCAGTGGAACTA GAAAATGGCTTAAATGCCGATGAGCATATGGCAAACACAGATTCTATATGCCAGCCATTAATAAAAGGAC GATCTGCCCCTGGAAGATTAAACCAGCCTTCTGCAGTGGGTACTCGTCAGCAAAAATTGACATCAGCATT CCAGCAGCAGCATTTGAACTTCAGTCAGGTTCAGCACTTTGGATCTGGGCATCAAGAGTGGAATGGAAAC TTTGGGCACAGAAGACAGCAAGCTTATATTCCTACTAGTGTTACCAGTAATCCATTCACTCTTTCTCATG GAAGTCCCAATCACACAGCAGTGCATGCCCACCTGGCTGGAAATACACACCTCGGAGGACAGCCTACTCT ACTTCCATACCCATCATCAGCCACCCTCAGTAGTGCTGCACCAGTGGCCCACCTGTTAGCCTCTCCGTGT ACCTCAAGACCTATGTTACAGCATCCAACTTATAATATCTCCCATCCCAGTGGCATAGTTCACCAAGTCC CAGTGGGCTTAAATCCCCGTCTGTTACCATCCCCAACCATTCATCAGACTCAGTACAAACCAATCTTCCC ACCACATTCTTACATTGCAGCATCACCTGCATATACTGGATTTCCACTGAGTCCAACAAAACTCAGCCAG TATCCATATATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG218848 representing NM_001048200
Red=Cloning site Green=Tags(s) MASQVLVYPPYVYQTQSSAFCSVKKLKVEPSSCVFQERNYPRTYVNGRNFGNSHPPTKGSAFQTKIPFNR PRGHNFSLQTSAVVLKNTAGATKVIAAQAQQAHVQAPQIGAWRNRLHFLEGPQRCGLKRKSEELDNHSSA MQIVDELSILPAMLQTNMGNPVTVVTATTGSKQNCTTGEGDYQLVQHEVLCSMKNTYEVLDFLGRGTFGQ VVKCWKRGTNEIVAIKILKNHPSYARQGQIEVSILARLSTENADEYNFVRAYECFQHRNHTCLVFEMLEQ NLYDFLKQNKFSPLPLKVIRPILQQVATALKKLKSLGLIHADLKPENIMLVDPVRQPYRVKVIDFGSASH VSKTVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGALEYDQIRYISQTQGLPGE QLLNVGTKSTRFFCKETDMSHSGWRLKTLEEHEAETGMKSKEARKYIFNSLDDVAHVNTVMDLEGSDLLA EKADRREFVSLLKKMLLIDADLRITPAETLNHPFVNMKHLLDFPHSNHVKSCFHIMDICKSHLNSCDTNN HNKTSLLRPVASSSTATLTANFTKIGTLRSQALTTSAHSVVHHGIPLQAGTAQFGCGDAFQQTLIICPPA IQGIPATHGKPTSYSIRVDNTVPLVTQAPAVQPLQIRPGVLSQTWSGRTQQMLVPAWQQVTPLAPATTTL TSESVAGSHRLGDWGKMISCSNHYNSVMPQPLLTNQITLSAPQPVSVGIAHVVWPQPATTKKNKQCQNRS NSLQNTNIPHSAFISPKIINGKDVEEVSCIETQDNQNSEGEARNCCETSIRQDSDSSVSDKQRQTIIIAD SPSPAVSVITISSDTDEEETSQRHSLRECKGSLDCEACQSTLNIDRMCSLSSPDSTLSTSSSGQSSPSPC KRPNSMSDEEQESSCDTVDGSPTSDSSGHDSPFAESTFVEDTHENTELVSSADTETKPAVCSVVVPPVEL ENGLNADEHMANTDSICQPLIKGRSAPGRLNQPSAVGTRQQKLTSAFQQQHLNFSQVQHFGSGHQEWNGN FGHRRQQAYIPTSVTSNPFTLSHGSPNHTAVHAHLAGNTHLGGQPTLLPYPSSATLSSAAPVAHLLASPC TSRPMLQHPTYNISHPSGIVHQVPVGLNPRLLPSPTIHQTQYKPIFPPHSYIAASPAYTGFPLSPTKLSQ YPYM TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001048200 |
| ORF Size | 3582 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001048200.3 |
| RefSeq Size | 4680 bp |
| RefSeq ORF | 3585 bp |
| Locus ID | 10114 |
| UniProt ID | Q9H422 |
| Protein Families | Druggable Genome, Protein Kinase, Transcription Factors |
| Gene Summary | Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation: phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218848 | HIPK3 (Myc-DDK-tagged)-Human homeodomain interacting protein kinase 3 (HIPK3), transcript variant 3 |
CNY 11,880.00 |
|
| RC218848L3 | Lenti-ORF clone of HIPK3 (Myc-DDK-tagged)-Human homeodomain interacting protein kinase 3 (HIPK3), transcript variant 3 |
CNY 13,780.00 |
|
| RC218848L4 | Lenti-ORF clone of HIPK3 (mGFP-tagged)-Human homeodomain interacting protein kinase 3 (HIPK3), transcript variant 3 |
CNY 13,780.00 |
|
| SC315459 | HIPK3 (untagged)-Human homeodomain interacting protein kinase 3 (HIPK3), transcript variant 3 |
CNY 22,990.00 |
