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STT3B (NM_178862) Human Tagged ORF Clone
CAT＃: RG218829
- TrueORF®
STT3B (tGFP-tagged) - Human STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) (STT3B). Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_178862" in other vectors (4)
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CNY 9,500.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CDG1X; SIMP; STT3-B |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG218829 ORF sequence, codon optimized.
Due to the complexity of NM_178862, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCCCTCGGCCCCGGAGAGCAAGCACAAGTCGTCCCTCAACTCGTCCCCGTGGAGTGGCCTCA TGGCCCTGGGAAACAGCCGGCACGGCCACCACGGGCCCGGGGCCCAGTGCGCGCACAAGGCGGCGGGCGG CGCGGCGCCGCCGAAGCCGGCCCCGGCGGGGCTGTCCGGGGGGCTGTCGCAGCCGGCTGGGTGGCAGTCG CTTCTCTCCTTCACCATCCTCTTCCTGGCCTGGCTTGCCGGCTTCAGCTCGCGCCTCTTCGCCGTCATCC GCTTCGAAAGCATCATCCACGAGTTCGACCCGTGGTTTAACTATAGATCAACACATCATCTTGCATCTCA TGGGTTCTATGAATTTTTAAATTGGTTTGATGAAAGAGCATGGTATCCACTAGGAAGAATAGTAGGTGGT ACTGTTTACCCAGGGTTGATGATAACCGCTGGCCTTATTCATTGGATTTTAAATACATTGAACATAACTG TTCACATAAGAGACGTATGTGTGTTCCTTGCACCAACTTTTAGCGGCCTTACATCTATATCTACTTTCCT GCTTACAAGAGAACTTTGGAACCAAGGAGCAGGACTTTTAGCTGCTTGTTTTATTGCTATTGTACCAGGC TACATATCTCGGTCAGTAGCTGGATCCTTTGATAATGAAGGCATTGCTATTTTTGCACTTCAGTTCACAT ACTATTTATGGGTAAAATCTGTAAAAACTGGGTCAGTTTTTTGGACAATGTGCTGCTGCTTATCCTATTT CTATATGGTCTCTGCTTGGGGTGGTTATGTATTTATCATCAATCTTATTCCACTGCATGTATTTGTGTTG TTACTGATGCAGAGATACAGCAAAAGAGTCTACATAGCATATAGCACTTTCTACATTGTGGGTTTAATAT TATCAATGCAGATACCTTTTGTGGGATTCCAGCCAATCAGAACAAGTGAACACATGGCAGCTGCAGGTGT CTTTGCATTGCTGCAAGCTTATGCTTTCTTGCAGTATCTGAGAGACCGATTAACAAAACAAGAGTTCCAG ACCCTTTTCTTTTTGGGTGTATCACTAGCTGCAGGTGCTGTGTTCCTTAGTGTCATCTATTTGACTTATA CAGGTTACATTGCACCATGGAGTGGCAGGTTTTATTCATTGTGGGATACTGGGTATGCAAAAATACACAT TCCAATTATTGCATCAGTGTCTGAGCATCAACCTACGACTTGGGTGTCTTTCTTCTTTGATCTACATATT CTCGTTTGTACTTTTCCCGCAGGATTGTGGTTCTGTATTAAAAACATTAACGATGAGCGCGTATTTGTGG CACTGTACGCCATTAGCGCAGTCTATTTTGCTGGTGTGATGGTCCGCCTCATGCTGACATTGACGCCGGT CGTTTGCATGCTCTCAGCTATTGCCTTCTCTAATGTGTTTGAACATTATCTCGGAGATGACATGAAAAGA GAAAACCCCCCAGTTGAGGACTCTTCCGACGAGGATGACAAAAGAAACCAGGGTAACCTTTACGACAAGG CCGGCAAAGTTCGCAAGCATGCTACAGAGCAAGAAAAAACAGAGGAGGGGCTGGGGCCAAACATTAAATC AATAGTAACAATGCTGATGCTCATGCTGCTTATGATGTTCGCGGTGCACTGTACATGGGTGACATCTAAT GCTTATTCTTCTCCTTCAGTAGTCTTGGCATCCTATAACCATGATGGAACACGGAACATTCTTGATGACT TCCGCGAGGCTTACTTCTGGTTGCGGCAAAATACGGACGAACACGCTCGGGTGATGAGCTGGTGGGATTA TGGATATCAAATCGCGGGCATGGCTAATAGGACTACTCTTGTTGATAATAATACTTGGAACAACTCACAC ATTGCACTTGTCGGTAAGGCCATGAGTTCCAACGAGACGGCTGCATACAAAATCATGCGAACACTGGATG TTGATTACGTGCTGGTAATTTTTGGCGGAGTGATAGGATATTCTGGCGATGACATAAACAAGTTTCTGTG GATGGTAAGGATTGCCGAGGGGGAGCATCCCAAAGATATACGAGAAAGCGATTACTTTACCCCACAAGGA GAATTCCGCGTGGACAAAGCCGGCTCCCCTACTCTCTTGAATTGCCTGATGTACAAAATGAGCTATTATC GATTTGGCGAAATGCAGCTCGATTTCAGAACACCCCCAGGCTTTGATAGAACCCGGAATGCAGAGATAGG TAACAAAGATATTAAATTCAAGCACCTGGAAGAAGCGTTTACCTCTGAACACTGGTTGGTCCGAATTTAT AAAGTCAAAGCTCCCGATAATCGCGAAACTCTTGACCATAAACCACGGGTGACTAATATATTCCCAAAGC AGAAATACCTTTCCAAGAAAACAACAAAGAGGAAGAGAGGTTACATAAAAAATAAGCTCGTTTTCAAAAA GGGTAAGAAGATCAGTAAGAAAACAGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG218829 representing NM_178862
Red=Cloning site Green=Tags(s) MAEPSAPESKHKSSLNSSPWSGLMALGNSRHGHHGPGAQCAHKAAGGAAPPKPAPAGLSGGLSQPAGWQS LLSFTILFLAWLAGFSSRLFAVIRFESIIHEFDPWFNYRSTHHLASHGFYEFLNWFDERAWYPLGRIVGG TVYPGLMITAGLIHWILNTLNITVHIRDVCVFLAPTFSGLTSISTFLLTRELWNQGAGLLAACFIAIVPG YISRSVAGSFDNEGIAIFALQFTYYLWVKSVKTGSVFWTMCCCLSYFYMVSAWGGYVFIINLIPLHVFVL LLMQRYSKRVYIAYSTFYIVGLILSMQIPFVGFQPIRTSEHMAAAGVFALLQAYAFLQYLRDRLTKQEFQ TLFFLGVSLAAGAVFLSVIYLTYTGYIAPWSGRFYSLWDTGYAKIHIPIIASVSEHQPTTWVSFFFDLHI LVCTFPAGLWFCIKNINDERVFVALYAISAVYFAGVMVRLMLTLTPVVCMLSAIAFSNVFEHYLGDDMKR ENPPVEDSSDEDDKRNQGNLYDKAGKVRKHATEQEKTEEGLGPNIKSIVTMLMLMLLMMFAVHCTWVTSN AYSSPSVVLASYNHDGTRNILDDFREAYFWLRQNTDEHARVMSWWDYGYQIAGMANRTTLVDNNTWNNSH IALVGKAMSSNETAAYKIMRTLDVDYVLVIFGGVIGYSGDDINKFLWMVRIAEGEHPKDIRESDYFTPQG EFRVDKAGSPTLLNCLMYKMSYYRFGEMQLDFRTPPGFDRTRNAEIGNKDIKFKHLEEAFTSEHWLVRIY KVKAPDNRETLDHKPRVTNIFPKQKYLSKKTTKRKRGYIKNKLVFKKGKKISKKTV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_178862 |
| ORF Size | 2478 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_178862.1, NP_849193.1 |
| RefSeq Size | 4553 bp |
| RefSeq ORF | 2481 bp |
| Locus ID | 201595 |
| UniProt ID | Q8TCJ2 |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, N-Glycan biosynthesis |
| Gene Summary | The protein encoded by this gene is a catalytic subunit of a protein complex that transfers oligosaccharides onto asparagine residues. Defects in this gene are a cause of congenital disorder of glycosylation Ix (CDG1X). [provided by RefSeq, Jun 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218829 | STT3B (Myc-DDK-tagged)-Human STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) (STT3B). Note: ORF is codon optimized |
CNY 8,650.00 |
|
| RC218829L3 | Lenti-ORF clone of STT3B (Myc-DDK-tagged)-Human STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) (STT3B). Note: ORF is codon optimized |
CNY 10,550.00 |
|
| RC218829L4 | Lenti-ORF clone of STT3B (mGFP-tagged)-Human STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) (STT3B). Note: ORF is codon optimized |
CNY 10,550.00 |
|
| SC107172 | STT3B (untagged)-Human STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) (STT3B) |
CNY 9,088.00 |
