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COL11A1 (NM_080630) Human Tagged ORF Clone
CAT＃: RG218205
- TrueORF®
COL11A1 (tGFP-tagged) - Human collagen, type XI, alpha 1 (COL11A1), transcript variant C
ORF Plasmid: DDK
"NM_080630" in other vectors (2)
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CNY 18,530.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CO11A1; COLL6; DFNA37; STL2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG218205 representing NM_080630
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGTGGTCCTCTAGGTGGAAAACGAAACGGTGGCTCTGGGATTTCACCGTAACAACCCTCGCAT TGACCTTCCTCTTCCAAGCTAGAGAGGTCAGAGGAGCTGCTCCAGTTGATGTACTAAAAGCACTAGATTT TCACAATTCTCCAGAGGGAATATCAAAAACAACGGGATTTTGCACAAACAGAAAGAATTCTAAAGGCTCA GATACTGCTTACAGAGTTTCAAAGCAAGCACAACTCAGTGCCCCAACAAAACAGTTATTTCCAGGTGGAA CTTTCCCAGAAGACTTTTCAATACTATTTACAGTAAAACCAAAAAAAGGAATTCAGTCTTTCCTTTTATC TATATATAATGAGCATGGTATTCAGCAAATTGGTGTTGAGGTTGGGAGATCACCTGTTTTTCTGTTTGAA GACCACACTGGAAAACCTGCCCCAGAAGACTATCCCCTCTTCAGAACTGTTAACATCGCTGACGGGAAGT GGCATCGGGTAGCAATCAGCGTGGAGAAGAAAACTGTGACAATGATTGTTGATTGTAAGAAGAAAACCAC GAAACCACTTGATAGAAGTGAGAGAGCAATTGTTGATACCAATGGAATCACGGTTTTTGGAACAAGGATT TTGGATGAAGAAGTTTTTGAGGGGGACATTCAGCAGTTTTTGATCACAGGTGATCCCAAGGCAGCATATG ACTACTGTGAGCATTATAGTCCAGACTGTGACTCTTCAGCACCCAAGGCTGCTCAAGCTCAGGAACCTCA GATAGATGAGTATGCACCAGAGGATATAATCGAATATGACTATGAGTATGGGGAAGCAGAGTATAAAGAG GCTGAAAGTGTAACAGAGGGACCCACTGTAACTGAGGAGACAATAGCACAGACGGAGATAAATGGCCATG GTGCATATGGAGAGAAAGGACAGAAAGGAGAACCAGCAGTGGTTGAGCCTGGTATGCTTGTCGAAGGACC ACCAGGACCAGCAGGACCTGCAGGTATTATGGGTCCTCCAGGTCTACAAGGCCCCACTGGACCCCCTGGT GACCCTGGCGATAGGGGCCCCCCAGGACGTCCTGGCTTACCAGGGGCTGATGGTCTACCTGGTCCTCCTG GTACTATGTTGATGTTACCGTTCCGTTATGGTGGTGATGGTTCCAAAGGACCAACCATCTCTGCTCAGGA AGCTCAGGCTCAAGCTATTCTTCAGCAGGCTCGGATTGCTCTGAGAGGCCCACCTGGCCCAATGGGTCTA ACTGGAAGACCAGGTCCTGTGGGGGGGCCTGGTTCATCTGGGGCCAAAGGTGAGAGTGGTGATCCAGGTC CTCAGGGCCCTCGAGGCGTCCAGGGTCCCCCTGGTCCAACGGGAAAACCTGGAAAAAGGGGTCGTCCAGG TGCAGATGGAGGAAGAGGAATGCCAGGAGAACCTGGGGCAAAGGGAGATCGAGGGTTTGATGGACTTCCG GGTCTGCCAGGTGACAAAGGTCACAGGGGTGAACGAGGTCCTCAAGGTCCTCCAGGTCCTCCTGGTGATG ATGGAATGAGGGGAGAAGATGGAGAAATTGGACCAAGAGGTCTTCCAGGTGAAGCTGGCCCACGAGGTTT GCTGGGTCCAAGGGGAACTCCAGGAGCTCCAGGGCAGCCTGGTATGGCAGGTGTAGATGGCCCCCCAGGA CCAAAAGGGAACATGGGTCCCCAAGGGGAGCCTGGGCCTCCAGGTCAACAAGGGAATCCAGGACCTCAGG GTCTTCCTGGTCCACAAGGTCCAATTGGTCCTCCTGGTGAAAAAGGACCACAAGGAAAACCAGGACTTGC TGGACTTCCTGGTGCTGATGGGCCTCCTGGTCATCCTGGGAAAGAAGGCCAGTCTGGAGAAAAGGGGGCT CTGGGTCCCCCTGGTCCACAAGGTCCTATTGGATACCCGGGCCCCCGGGGAGTAAAGGGAGCAGATGGTG TCAGAGGTCTCAAGGGATCTAAAGGTGAAAAGGGTGAAGATGGTTTTCCAGGATTCAAAGGTGACATGGG TCTAAAAGGTGACAGAGGAGAAGTTGGTCAAATTGGCCCAAGAGGGGAAGATGGCCCTGAAGGACCCAAA GGTCGAGCAGGCCCAACTGGAGACCCAGGTCCTTCAGGTCAAGCAGGAGAAAAGGGAAAACTTGGAGTTC CAGGATTACCAGGATATCCAGGAAGACAAGGTCCAAAGGGTTCCACTGGATTCCCTGGGTTTCCAGGTGC CAATGGAGAGAAAGGTGCACGGGGAGTAGCTGGCAAACCAGGCCCTCGGGGTCAGCGTGGTCCAACGGGT CCTCGAGGTTCAAGAGGTGCAAGAGGTCCCACTGGGAAACCTGGGCCAAAGGGCACTTCAGGTGGCGATG GCCCTCCTGGCCCTCCAGGTGAAAGAGGTCCTCAAGGACCTCAGGGTCCAGTTGGATTCCCTGGACCAAA AGGCCCTCCTGGACCACCTGGGAAGGATGGGCTGCCAGGACACCCTGGGCAACGTGGGGAGACTGGATTT CAAGGCAAGACCGGCCCTCCTGGGCCAGGGGGAGTGGTTGGACCACAGGGACCAACCGGTGAGACTGGTC CAATAGGGGAACGTGGGCATCCTGGCCCTCCTGGCCCTCCTGGTGAGCAAGGTCTTCCTGGTGCTGCAGG AAAAGAAGGTGCAAAGGGTGATCCAGGTCCTCAAGGTATCTCAGGGAAAGATGGACCAGCAGGATTACGT GGTTTCCCAGGGGAAAGAGGTCTTCCTGGAGCTCAGGGTGCACCTGGACTGAAAGGAGGGGAAGGTCCCC AGGGCCCACCAGGTCCAGTTGGCTCACCAGGAGAACGTGGGTCAGCAGGTACAGCTGGCCCAATTGGTTT ACCAGGGCGCCCGGGACCTCAGGGTCCTCCTGGTCCAGCTGGAGAGAAAGGTGCTCCTGGAGAAAAAGGT CCCCAAGGGCCTGCAGGGAGAGATGGAGTTCAAGGTCCTGTTGGTCTCCCAGGGCCAGCTGGTCCTGCCG GCTCCCCTGGGGAAGACGGAGACAAGGGTGAAATTGGTGAGCCGGGACAAAAAGGCAGCAAGGGTGACAA GGGAGAAAATGGCCCTCCCGGTCCCCCAGGTCTTCAAGGACCAGTTGGTGCCCCTGGAATTGCTGGAGGT GATGGTGAACCAGGTCCTAGAGGACAGCAGGGGATGTTTGGGCAAAAAGGTGATGAGGGTGCCAGAGGCT TCCCTGGACCTCCTGGTCCAATAGGTCTTCAGGGTCTGCCAGGCCCACCTGGTGAAAAAGGTGAAAATGG GGATGTTGGTCCCATGGGGCCACCTGGTCCTCCAGGCCCAAGAGGCCCTCAAGGTCCCAATGGAGCTGAT GGACCACAAGGACCCCCAGGGTCTGTTGGTTCAGTTGGTGGTGTTGGAGAAAAGGGTGAACCTGGAGAAG CAGGGAACCCAGGGCCTCCTGGGGAAGCAGGTGTAGGCGGTCCCAAAGGAGAAAGAGGAGAGAAAGGGGA AGCTGGTCCACCTGGAGCTGCTGGACCTCCAGGTGCCAAGGGGCCACCAGGTGATGATGGCCCTAAGGGT AACCCGGGTCCTGTTGGTTTTCCTGGAGATCCTGGTCCTCCTGGGGAACCTGGCCCTGCAGGTCAAGATG GTGTTGGTGGTGACAAGGGTGAAGATGGAGATCCTGGTCAACCGGGTCCTCCTGGCCCATCTGGTGAGGC TGGCCCACCAGGTCCTCCTGGAAAACGAGGTCCTCCTGGAGCTGCAGGTGCAGAGGGAAGACAAGGTGAA AAAGGTGCTAAGGGGGAAGCAGGTGCAGAAGGTCCTCCTGGAAAAACCGGCCCAGTCGGTCCTCAGGGAC CTGCAGGAAAGCCTGGTCCAGAAGGTCTTCGGGGCATCCCTGGTCCTGTGGGAGAACAAGGTCTCCCTGG AGCTGCAGGCCAAGATGGACCACCTGGTCCTATGGGACCTCCTGGCTTACCTGGTCTCAAAGGTGACCCT GGCTCCAAGGGTGAAAAGGGACATCCTGGTTTAATTGGCCTGATTGGTCCTCCAGGAGAACAAGGGGAAA AAGGTGACCGAGGGCTCCCTGGAACTCAAGGATCTCCAGGAGCAAAAGGGGATGGGGGAATTCCTGGTCC TGCTGGTCCCTTAGGTCCACCTGGTCCTCCAGGTTTACCAGGTCCTCAAGGCCCAAAGGGTAACAAAGGC TCTACTGGACCCGCTGGCCAGAAAGGTGACAGTGGTCTTCCAGGGCCTCCTGGGTCTCCAGGTCCACCTG GTGAAGTCATTCAGCCTTTACCAATCTTGTCCTCCAAAAAAACGAGAAGACATACTGAAGGCATGCAAGC AGATGCAGATGATAATATTCTTGATTACTCGGATGGAATGGAAGAAATATTTGGTTCCCTCAATTCCCTG AAACAAGACATTGAGCATATGAAATTTCCAATGGGTACTCAGACCAATCCAGCCCGAACTTGTAAAGACC TGCAACTCAGCCATCCTGACTTCCCAGATGGTGAATATTGGATTGATCCTAACCAAGGTTGCTCAGGAGA TTCCTTCAAAGTTTACTGTAATTTCACATCTGGTGGTGAGACTTGCATTTATCCAGACAAAAAATCTGAG GGAGTAAGAATTTCATCATGGCCAAAGGAGAAACCAGGAAGTTGGTTTAGTGAATTTAAGAGGGGAAAAC TGCTTTCATACTTAGATGTTGAAGGAAATTCCATCAATATGGTGCAAATGACATTCCTGAAACTTCTGAC TGCCTCTGCTCGGCAAAATTTCACCTACCACTGTCATCAGTCAGCAGCCTGGTATGATGTGTCATCAGGA AGTTATGACAAAGCACTTCGCTTCCTGGGATCAAATGATGAGGAGATGTCCTATGACAATAATCCTTTTA TCAAAACACTGTATGATGGTTGTGCGTCCAGAAAAGGCTATGAAAAGACTGTCATTGAAATCAATACACC AAAAATTGATCAAGTACCTATTGTTGATGTCATGATCAATGACTTTGGTGATCAGAATCAGAAGTTCGGA TTTGAAGTTGGTCCTGTTTGTTTTCTTGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG218205 representing NM_080630
Red=Cloning site Green=Tags(s) MEPWSSRWKTKRWLWDFTVTTLALTFLFQAREVRGAAPVDVLKALDFHNSPEGISKTTGFCTNRKNSKGS DTAYRVSKQAQLSAPTKQLFPGGTFPEDFSILFTVKPKKGIQSFLLSIYNEHGIQQIGVEVGRSPVFLFE DHTGKPAPEDYPLFRTVNIADGKWHRVAISVEKKTVTMIVDCKKKTTKPLDRSERAIVDTNGITVFGTRI LDEEVFEGDIQQFLITGDPKAAYDYCEHYSPDCDSSAPKAAQAQEPQIDEYAPEDIIEYDYEYGEAEYKE AESVTEGPTVTEETIAQTEINGHGAYGEKGQKGEPAVVEPGMLVEGPPGPAGPAGIMGPPGLQGPTGPPG DPGDRGPPGRPGLPGADGLPGPPGTMLMLPFRYGGDGSKGPTISAQEAQAQAILQQARIALRGPPGPMGL TGRPGPVGGPGSSGAKGESGDPGPQGPRGVQGPPGPTGKPGKRGRPGADGGRGMPGEPGAKGDRGFDGLP GLPGDKGHRGERGPQGPPGPPGDDGMRGEDGEIGPRGLPGEAGPRGLLGPRGTPGAPGQPGMAGVDGPPG PKGNMGPQGEPGPPGQQGNPGPQGLPGPQGPIGPPGEKGPQGKPGLAGLPGADGPPGHPGKEGQSGEKGA LGPPGPQGPIGYPGPRGVKGADGVRGLKGSKGEKGEDGFPGFKGDMGLKGDRGEVGQIGPRGEDGPEGPK GRAGPTGDPGPSGQAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGARGVAGKPGPRGQRGPTG PRGSRGARGPTGKPGPKGTSGGDGPPGPPGERGPQGPQGPVGFPGPKGPPGPPGKDGLPGHPGQRGETGF QGKTGPPGPGGVVGPQGPTGETGPIGERGHPGPPGPPGEQGLPGAAGKEGAKGDPGPQGISGKDGPAGLR GFPGERGLPGAQGAPGLKGGEGPQGPPGPVGSPGERGSAGTAGPIGLPGRPGPQGPPGPAGEKGAPGEKG PQGPAGRDGVQGPVGLPGPAGPAGSPGEDGDKGEIGEPGQKGSKGDKGENGPPGPPGLQGPVGAPGIAGG DGEPGPRGQQGMFGQKGDEGARGFPGPPGPIGLQGLPGPPGEKGENGDVGPMGPPGPPGPRGPQGPNGAD GPQGPPGSVGSVGGVGEKGEPGEAGNPGPPGEAGVGGPKGERGEKGEAGPPGAAGPPGAKGPPGDDGPKG NPGPVGFPGDPGPPGEPGPAGQDGVGGDKGEDGDPGQPGPPGPSGEAGPPGPPGKRGPPGAAGAEGRQGE KGAKGEAGAEGPPGKTGPVGPQGPAGKPGPEGLRGIPGPVGEQGLPGAAGQDGPPGPMGPPGLPGLKGDP GSKGEKGHPGLIGLIGPPGEQGEKGDRGLPGTQGSPGAKGDGGIPGPAGPLGPPGPPGLPGPQGPKGNKG STGPAGQKGDSGLPGPPGSPGPPGEVIQPLPILSSKKTRRHTEGMQADADDNILDYSDGMEEIFGSLNSL KQDIEHMKFPMGTQTNPARTCKDLQLSHPDFPDGEYWIDPNQGCSGDSFKVYCNFTSGGETCIYPDKKSE GVRISSWPKEKPGSWFSEFKRGKLLSYLDVEGNSINMVQMTFLKLLTASARQNFTYHCHQSAAWYDVSSG SYDKALRFLGSNDEEMSYDNNPFIKTLYDGCASRKGYEKTVIEINTPKIDQVPIVDVMINDFGDQNQKFG FEVGPVCFLG TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_080630 |
| ORF Size | 5070 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_080630.4 |
| RefSeq Size | 6960 bp |
| RefSeq ORF | 5073 bp |
| Locus ID | 1301 |
| UniProt ID | P12107 |
| Protein Pathways | ECM-receptor interaction, Focal adhesion |
| Gene Summary | This gene encodes one of the two alpha chains of type XI collagen, a minor fibrillar collagen. Type XI collagen is a heterotrimer but the third alpha chain is a post-translationally modified alpha 1 type II chain. Mutations in this gene are associated with type II Stickler syndrome and with Marshall syndrome. A single-nucleotide polymorphism in this gene is also associated with susceptibility to lumbar disc herniation. Multiple transcript variants have been identified for this gene. [provided by RefSeq, Nov 2009] |
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