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AUH (NM_001698) Human Tagged ORF Clone
CAT＃: RG218117
- TrueORF®
AUH (tGFP-tagged) - Human AU RNA binding protein/enoyl-CoA hydratase (AUH), nuclear gene encoding mitochondrial protein
ORF Plasmid: DDK
"NM_001698" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG218117 representing NM_001698
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCGCGGTGGCGGCGGCACCTGGGGCCTTGGGATCCCTGCATGCTGGCGGCGCCCGCCTGGTGG CCGCTTGCAGTGCGTGGCTCTGCCCGGGGTTGAGGCTGCCCGGCTCGTTGGCAGGCCGGCGAGCGGGCCC GGCGATCTGGGCCCAGGGCTGGGTACCTGCGGCCGGGGGTCCCGCCCCGAAAAGGGGCTACAGCTCTGAG ATGAAGACGGAGGACGAGCTGCGGGTGCGGCACCTGGAGGAGGAGAACCGAGGAATTGTGGTGCTTGGAA TAAACAGAGCTTATGGCAAAAATTCACTCAGTAAAAATCTTATAAAAATGCTATCAAAAGCTGTGGATGC TTTGAAATCTGATAAGAAAGTACGGACCATAATAATCAGGAGTGAAGTCCCAGGGATATTCTGTGCTGGT GCTGACCTTAAGGAAAGAGCCAAAATGAGTTCCAGTGAAGTTGGTCCTTTTGTCTCCAAAATAAGAGCAG TGATTAACGATATTGCTAATCTTCCAGTGCCAACAATTGCAGCAATAGATGGACTCGCTTTAGGTGGTGG TCTTGAACTGGCTTTAGCCTGTGATATACGAGTAGCAGCTTCCTCTGCAAAAATGGGCCTGGTTGAAACA AAATTGGCGATTATTCCTGGTGGAGGGGGGACACAGCGATTGCCACGCGCCATTGGAATGTCCCTGGCCA AGGAGCTCATATTCTCTGCGCGAGTCCTCGATGGCAAAGAAGCCAAAGCAGTGGGCTTAATCAGCCACGT TCTGGAACAGAACCAGGAGGGAGACGCGGCCTACAGGAAGGCCTTGGACCTGGCGAGAGAGTTTTTACCT CAGGGACCTGTTGCAATGAGAGTGGCAAAATTAGCAATTAATCAAGGGATGGAGGTCGATTTAGTAACAG GGTTAGCCATAGAAGAAGCTTGTTATGCTCAGACCATTCCAACAAAAGACAGACTTGAAGGTCTTCTTGC TTTTAAAGAGAAAAGGCCCCCTCGCTATAAAGGAGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG218117 representing NM_001698
Red=Cloning site Green=Tags(s) MAAAVAAAPGALGSLHAGGARLVAACSAWLCPGLRLPGSLAGRRAGPAIWAQGWVPAAGGPAPKRGYSSE MKTEDELRVRHLEEENRGIVVLGINRAYGKNSLSKNLIKMLSKAVDALKSDKKVRTIIIRSEVPGIFCAG ADLKERAKMSSSEVGPFVSKIRAVINDIANLPVPTIAAIDGLALGGGLELALACDIRVAASSAKMGLVET KLAIIPGGGGTQRLPRAIGMSLAKELIFSARVLDGKEAKAVGLISHVLEQNQEGDAAYRKALDLAREFLP QGPVAMRVAKLAINQGMEVDLVTGLAIEEACYAQTIPTKDRLEGLLAFKEKRPPRYKGE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001698 |
| ORF Size | 1017 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001698.1, NP_001689.1 |
| RefSeq Size | 1548 bp |
| RefSeq ORF | 1020 bp |
| Locus ID | 549 |
| UniProt ID | Q13825 |
| Domains | ECH |
| Protein Pathways | Metabolic pathways, Valine, leucine and isoleucine degradation |
| Gene Summary | This gene encodes bifunctional mitochondrial protein that has both RNA-binding and hydratase activities. The encoded protein is a methylglutaconyl-CoA hydratase that catalyzes the hydration of 3-methylglutaconyl-CoA to 3-hydroxy-3-methyl-glutaryl-CoA, a critical step in the leucine degradation pathway. This protein also binds AU-rich elements (AREs) found in the 3' UTRs of rapidly decaying mRNAs including c-fos, c-myc and granulocyte/ macrophage colony stimulating factor. ARE elements are involved in directing RNA to rapid degradation and deadenylation. This protein is localizes to the mitochondrial matrix and the inner mitochondrial membrane and may be involved in mitochondrial protein synthesis. Mutations in this gene are the cause of 3-methylglutaconic aciduria, type I. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218117 | AUH (Myc-DDK-tagged)-Human AU RNA binding protein/enoyl-CoA hydratase (AUH), nuclear gene encoding mitochondrial protein |
CNY 5,488.00 |
|
| RC218117L3 | Lenti ORF clone of Human AU RNA binding protein/enoyl-CoA hydratase (AUH), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC218117L4 | Lenti ORF clone of Human AU RNA binding protein/enoyl-CoA hydratase (AUH), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
| SC313016 | AUH (untagged)-Human AU RNA binding protein/enoyl-CoA hydratase (AUH), nuclear gene encoding mitochondrial protein |
CNY 5,488.00 |
