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TRPM7 (NM_017672) Human Tagged ORF Clone
CAT＃: RG218043
- TrueORF®
TRPM7 (tGFP-tagged) - Human transient receptor potential cation channel, subfamily M, member 7 (TRPM7)
ORF Plasmid: DDK
"NM_017672" in other vectors (4)
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CNY 20,520.00
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7; TRP-PLIK |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG218043 representing NM_017672
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCAGAAATCCTGGATAGAAAGCACTTTGACCAAGAGGGAATGTGTATATATTATACCAAGTTCCA AGGACCCTCACAGATGCCTTCCAGGATGTCAAATTTGTCAGCAACTCGTCAGGTGTTTTTGTGGTCGCTT GGTCAAGCAACATGCTTGTTTTACTGCAAGTCTTGCCATGAAATACTCAGATGTGAAATTGGGTGACCAT TTTAATCAGGCAATAGAAGAATGGTCTGTGGAAAAGCATACAGAACAGAGCCCAACGGATGCTTATGGAG TCATAAATTTTCAAGGGGGTTCTCATTCCTACAGAGCTAAGTATGTGAGGCTATCATATGACACCAAACC TGAAGTCATTCTGCAACTTCTGCTTAAAGAATGGCAAATGGAGTTACCCAAACTTGTTATCTCTGTACAT GGGGGCATGCAGAAATTTGAGCTTCACCCACGAATCAAGCAGTTGCTTGGAAAAGGTCTTATTAAAGCTG CAGTTACAACTGGAGCCTGGATTTTAACTGGAGGAGTAAACACAGGTGTGGCAAAACATGTTGGAGATGC CCTCAAAGAACATGCTTCCAGATCATCTCGAAAGATTTGCACTATCGGAATAGCTCCATGGGGAGTGATT GAAAACAGAAATGATCTTGTTGGGAGAGATGTGGTTGCTCCTTATCAAACCTTATTGAACCCCCTGAGCA AATTGAATGTTTTGAATAATCTGCATTCCCATTTCATATTGGTGGATGATGGCACTGTTGGAAAGTATGG GGCGGAAGTCAGACTGAGAAGAGAACTTGAAAAAACTATTAATCAGCAAAGAATTCATGCTAGGATTGGC CAGGGTGTCCCTGTGGTGGCACTTATATTTGAGGGTGGGCCAAATGTTATCCTCACAGTTCTTGAATACC TTCAGGAAAGCCCCCCTGTTCCAGTAGTTGTGTGTGAAGGAACAGGCAGAGCTGCAGATCTGCTAGCGTA TATTCATAAACAAACAGAAGAAGGAGGGAATCTTCCTGATGCAGCAGAGCCCGATATTATTTCCACTATC AAAAAAACATTTAACTTTGGCCAGAATGAAGCACTTCATTTATTTCAAACACTGATGGAGTGCATGAAAA GAAAGGAGCTTATCACTGTTTTCCATATTGGGTCAGATGAACATCAAGATATAGATGTAGCAATACTTAC TGCACTGCTAAAAGGTACTAATGCATCTGCATTTGACCAGCTTATCCTTACATTGGCATGGGATAGAGTT GACATTGCCAAAAATCATGTATTTGTTTATGGACAGCAGTGGCTGGTTGGATCCTTGGAACAAGCTATGC TTGATGCTCTTGTAATGGATAGAGTTGCATTTGTAAAACTTCTTATTGAAAATGGAGTAAGCATGCATAA ATTCCTTACCATTCCGAGACTGGAAGAACTTTACAACACTAAACAAGGTCCAACTAATCCAATGCTGTTT CATCTTGTTCGAGACGTCAAACAGGGAAATCTTCCTCCAGGATATAAGATCACTCTGATTGATATAGGAC TTGTTATTGAATATCTCATGGGAGGAACCTACAGATGCACCTATACTAGGAAACGTTTTCGATTAATATA TAATAGTCTTGGTGGAAATAATCGGAGGTCTGGCCGAAATACCTCCAGCAGCACTCCTCAGTTGCGAAAG AGTCATGAATCTTTTGGCAATAGGGCAGATAAAAAGGAAAAAATGAGGCATAACCATTTCATTAAGACAG CACAGCCCTACCGACCAAAGATTGATACAGTTATGGAAGAAGGAAAGAAGAAAAGAACCAAAGATGAAAT TGTAGACATTGATGATCCAGAAACCAAGCGCTTTCCTTATCCACTTAATGAACTTTTAATTTGGGCTTGC CTTATGAAGAGGCAGGTCATGGCCCGTTTTTTATGGCAACATGGTGAAGAATCAATGGCTAAAGCATTAG TTGCCTGTAAGATCTATCGTTCAATGGCATATGAAGCAAAGCAGAGTGACCTGGTAGATGATACTTCAGA AGAACTAAAACAGTATTCCAATGATTTTGGTCAGTTGGCCGTTGAATTATTAGAACAGTCCTTCAGACAA GATGAAACCATGGCTATGAAATTGCTCACTTATGAACTGAAGAACTGGAGTAATTCAACCTGCCTTAAGT TAGCAGTTTCTTCAAGACTTAGACCTTTTGTAGCTCACACCTGTACACAAATGTTGTTATCTGATATGTG GATGGGAAGGCTGAATATGAGGAAAAATTCCTGGTACAAGGTCATACTAAGCATTTTAGTTCCACCTGCC ATATTGCTGTTAGAGTATAAAACTAAGGCTGAAATGTCCCATATCCCACAATCTCAAGATGCTCATCAGA TGACAATGGATGACAGCGAAAACAACTTTCAGAACATAACAGAAGAGATCCCCATGGAAGTGTTTAAAGA AGTACGGATTTTGGATAGTAATGAAGGAAAGAATGAGATGGAGATACAAATGAAATCAAAAAAGCTTCCA ATTACGCGAAAGTTTTATGCCTTTTATCATGCACCAATTGTAAAATTCTGGTTTAACACGTTGGCATATT TAGGATTTCTGATGCTTTATACATTTGTGGTTCTTGTACAAATGGAACAGTTACCTTCAGTTCAAGAATG GATTGTTATTGCTTATATTTTTACTTATGCCATTGAGAAAGTCCGTGAGATCTTTATGTCTGAAGCTGGG AAAGTAAACCAGAAGATTAAAGTATGGTTTAGTGATTACTTCAACATCAGTGATACAATTGCCATAATTT CTTTCTTCATTGGATTTGGACTAAGATTTGGAGCAAAATGGAACTTTGCAAATGCATATGATAATCATGT TTTTGTGGCTGGAAGATTAATTTACTGTCTTAACATAATATTTTGGTATGTGCGTTTGCTAGATTTTCTA GCTGTAAATCAACAGGCAGGACCTTATGTAATGATGATTGGAAAAATGGTGGCCAATATGTTCTACATTG TAGTGATTATGGCTCTTGTATTACTTAGTTTTGGTGTTCCCAGAAAGGCAATACTTTATCCTCATGAAGC ACCATCTTGGACTCTTGCTAAAGATATAGTTTTTCACCCATACTGGATGATTTTTGGTGAAGTTTATGCA TACGAAATTGATGTGTGTGCAAATGATTCTGTTATCCCTCAAATCTGTGGTCCTGGGACGTGGTTGACTC CATTTCTTCAAGCAGTCTACCTCTTTGTACAGTATATCATTATGGTTAATCTTCTTATTGCATTTTTCAA CAATGTGTATTTACAAGTGAAGGCAATTTCCAATATTGTATGGAAGTACCAGCGTTATCATTTTATTATG GCTTATCATGAGAAACCAGTTCTGCCTCCTCCACTTATCATTCTTAGCCATATAGTTTCTCTGTTTTGCT GCATATGTAAGAGAAGAAAGAAAGATAAGACTTCCGATGGACCAAAACTTTTCTTAACAGAAGAAGATCA AAAGAAACTTCATGATTTTGAAGAGCAGTGTGTTGAAATGTATTTCAATGAAAAAGATGACAAATTTCAT TCTGGGAGTGAAGAGAGAATTCGTGTCACTTTTGAAAGAGTGGAACAGATGTGCATTCAGATTAAAGAAG TTGGAGATCGTGTCAACTACATAAAAAGATCATTACAATCATTAGATTCTCAAATTGGCCATTTGCAAGA TCTTTCAGCCCTGACGGTAGATACATTAAAAACACTCACTGCCCAGAAAGCGTCGGAAGCTAGCAAAGTT CATAATGAAATCACACGAGAACTGAGCATTTCCAAACACTTGGCTCAAAACCTTATTGATGATGGTCCTG TAAGACCTTCTGTATGGAAAAAGCATGGTGTTGTAAATACACTTAGCTCCTCTCTTCCTCAAGGTGATCT TGAAAGTAATAATCCTTTTCATTGTAATATTTTAATGAAAGATGACAAAGATCCCCAGTGTAATATATTT GGTCAAGACTTACCTGCAGTACCCCAGAGAAAAGAATTTAATTTTCCAGAGGCTGGTTCCTCTTCTGGTG CCTTATTCCCAAGTGCTGTTTCCCCTCCAGAACTGCGACAGAGACTACATGGGGTAGAACTCTTAAAAAT ATTTAATAAAAATCAAAAATTAGGCAGTTCATCTACTAGCATACCACATCTGTCATCCCCACCAACCAAA TTTTTTGTTAGTACACCATCTCAGCCAAGTTGCAAAAGCCACTTGGAAACTGGAACCAAAGATCAAGAAA CTGTTTGCTCTAAAGCTACAGAAGGAGATAATACAGAATTTGGAGCATTTGTAGGACACAGAGATAGCAT GGATTTACAGAGGTTTAAAGAAACATCAAACAAGATAAAAATACTATCCAATAACAATACTTCTGAAAAC ACTTTGAAACGAGTGAGTTCTCTTGCTGGATTTACTGACTGTCACAGAACTTCCATTCCTGTTCATTCAA AACAAGCAGAAAAAATCAGTAGAAGGCCATCTACCGAAGACACTCATGAAGTAGATTCCAAAGCAGCTTT AATACCGGATTGGTTACAAGATAGACCATCAAACAGAGAAATGCCATCTGAAGAAGGAACATTAAATGGT CTCACTTCTCCATTTAAGCCAGCTATGGATACAAATTACTATTATTCAGCTGTGGAAAGAAATAACTTGA TGAGGTTATCACAGAGCATTCCATTTACACCTGTGCCTCCAAGAGGGGAGCCTGTCACAGTGTATCGTTT GGAAGAGAGTTCACCCAACATACTAAATAACAGCATGTCTTCTTGGTCACAACTAGGCCTCTGTGCCAAA ATAGAGTTTTTAAGCAAAGAGGAGATGGGAGGAGGTTTACGAAGAGCTGTCAAAGTACAGTGTACCTGGT CAGAACATGATATCCTCAAATCAGGGCATCTTTATATTATCAAATCTTTTCTTCCAGAGGTGGTTAATAC ATGGTCAAGTATTTACAAAGAAGATACAGTTCTGCATCTCTGTCTGAGAGAAATTCAACAACAGAGAGCA GCACAAAAGCTTACGTTTGCCTTTAATCAAATGAAACCCAAATCCATACCATATTCTCCAAGGTTCCTTG AAGTTTTCCTGCTGTATTGCCATTCAGCAGGACAGTGGTTTGCTGTGGAAGAATGTATGACTGGAGAATT TAGAAAATACAACAATAATAATGGAGATGAGATTATTCCAACTAATACTCTGGAAGAGATCATGCTAGCC TTTAGCCACTGGACTTACGAATATACAAGAGGGGAGTTACTGGTACTTGATTTGCAAGGTGTTGGTGAAA ATTTGACTGACCCATCTGTGATAAAAGCAGAAGAAAAGAGATCCTGTGATATGGTTTTTGGCCCAGCAAA TCTAGGAGAAGATGCAATTAAAAACTTCAGAGCAAAACATCACTGTAATTCTTGCTGTAGAAAGCTTAAA CTTCCAGATCTGAAGAGGAATGATTATACGCCTGATAAAATTATATTTCCTCAGGATGAGCCTTCAGATT TGAATCTTCAGCCTGGAAATTCCACCAAAGAATCAGAATCAACTAATTCTGTTCGTCTGATGTTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG218043 representing NM_017672
Red=Cloning site Green=Tags(s) MSQKSWIESTLTKRECVYIIPSSKDPHRCLPGCQICQQLVRCFCGRLVKQHACFTASLAMKYSDVKLGDH FNQAIEEWSVEKHTEQSPTDAYGVINFQGGSHSYRAKYVRLSYDTKPEVILQLLLKEWQMELPKLVISVH GGMQKFELHPRIKQLLGKGLIKAAVTTGAWILTGGVNTGVAKHVGDALKEHASRSSRKICTIGIAPWGVI ENRNDLVGRDVVAPYQTLLNPLSKLNVLNNLHSHFILVDDGTVGKYGAEVRLRRELEKTINQQRIHARIG QGVPVVALIFEGGPNVILTVLEYLQESPPVPVVVCEGTGRAADLLAYIHKQTEEGGNLPDAAEPDIISTI KKTFNFGQNEALHLFQTLMECMKRKELITVFHIGSDEHQDIDVAILTALLKGTNASAFDQLILTLAWDRV DIAKNHVFVYGQQWLVGSLEQAMLDALVMDRVAFVKLLIENGVSMHKFLTIPRLEELYNTKQGPTNPMLF HLVRDVKQGNLPPGYKITLIDIGLVIEYLMGGTYRCTYTRKRFRLIYNSLGGNNRRSGRNTSSSTPQLRK SHESFGNRADKKEKMRHNHFIKTAQPYRPKIDTVMEEGKKKRTKDEIVDIDDPETKRFPYPLNELLIWAC LMKRQVMARFLWQHGEESMAKALVACKIYRSMAYEAKQSDLVDDTSEELKQYSNDFGQLAVELLEQSFRQ DETMAMKLLTYELKNWSNSTCLKLAVSSRLRPFVAHTCTQMLLSDMWMGRLNMRKNSWYKVILSILVPPA ILLLEYKTKAEMSHIPQSQDAHQMTMDDSENNFQNITEEIPMEVFKEVRILDSNEGKNEMEIQMKSKKLP ITRKFYAFYHAPIVKFWFNTLAYLGFLMLYTFVVLVQMEQLPSVQEWIVIAYIFTYAIEKVREIFMSEAG KVNQKIKVWFSDYFNISDTIAIISFFIGFGLRFGAKWNFANAYDNHVFVAGRLIYCLNIIFWYVRLLDFL AVNQQAGPYVMMIGKMVANMFYIVVIMALVLLSFGVPRKAILYPHEAPSWTLAKDIVFHPYWMIFGEVYA YEIDVCANDSVIPQICGPGTWLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLQVKAISNIVWKYQRYHFIM AYHEKPVLPPPLIILSHIVSLFCCICKRRKKDKTSDGPKLFLTEEDQKKLHDFEEQCVEMYFNEKDDKFH SGSEERIRVTFERVEQMCIQIKEVGDRVNYIKRSLQSLDSQIGHLQDLSALTVDTLKTLTAQKASEASKV HNEITRELSISKHLAQNLIDDGPVRPSVWKKHGVVNTLSSSLPQGDLESNNPFHCNILMKDDKDPQCNIF GQDLPAVPQRKEFNFPEAGSSSGALFPSAVSPPELRQRLHGVELLKIFNKNQKLGSSSTSIPHLSSPPTK FFVSTPSQPSCKSHLETGTKDQETVCSKATEGDNTEFGAFVGHRDSMDLQRFKETSNKIKILSNNNTSEN TLKRVSSLAGFTDCHRTSIPVHSKQAEKISRRPSTEDTHEVDSKAALIPDWLQDRPSNREMPSEEGTLNG LTSPFKPAMDTNYYYSAVERNNLMRLSQSIPFTPVPPRGEPVTVYRLEESSPNILNNSMSSWSQLGLCAK IEFLSKEEMGGGLRRAVKVQCTWSEHDILKSGHLYIIKSFLPEVVNTWSSIYKEDTVLHLCLREIQQQRA AQKLTFAFNQMKPKSIPYSPRFLEVFLLYCHSAGQWFAVEECMTGEFRKYNNNNGDEIIPTNTLEEIMLA FSHWTYEYTRGELLVLDLQGVGENLTDPSVIKAEEKRSCDMVFGPANLGEDAIKNFRAKHHCNSCCRKLK LPDLKRNDYTPDKIIFPQDEPSDLNLQPGNSTKESESTNSVRLML TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017672 |
| ORF Size | 5595 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017672.6 |
| RefSeq Size | 7255 bp |
| RefSeq ORF | 5598 bp |
| Locus ID | 54822 |
| UniProt ID | Q96QT4 |
| Protein Families | Druggable Genome, Ion Channels: Transient receptor potential, Protein Kinase, Transmembrane |
| Gene Summary | This gene belongs to the melastatin subfamily of transient receptor potential family of ion channels. The protein encoded by this gene is both an ion channel and a serine/threonine protein kinase. The kinase activity is essential for the ion channel function, which serves to increase intracellular calcium levels and to help regulate magnesium ion homeostasis. The encoded protein is involved in cytoskeletal organization, cell adhesion, cell migration and organogenesis. Defects in this gene are a cause of amyotrophic lateral sclerosis-parkinsonism/dementia complex of Guam. The gene may also be associated with defects of cardiac function. [provided by RefSeq, Aug 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218043 | TRPM7 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily M, member 7 (TRPM7) |
CNY 18,800.00 |
|
| RC218043L1 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily M, member 7 (TRPM7), Myc-DDK-tagged |
CNY 20,520.00 |
|
| SC304505 | TRPM7 (untagged)-Human transient receptor potential cation channel, subfamily M, member 7 (TRPM7) |
CNY 35,910.00 |
|
| SC318683 | TRPM7 (untagged)-Human transient receptor potential cation channel, subfamily M, member 7 (TRPM7) |
CNY 35,910.00 |
