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Cyclin B3 (CCNB3) (NM_033031) Human Tagged ORF Clone
CAT＃: RG217591
- TrueORF®
CCNB3 (tGFP-tagged) - Human cyclin B3 (CCNB3), transcript variant 3
ORF Plasmid: DDK
"NM_033031" in other vectors (4)
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CNY 15,300.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CYCB3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG217591 representing NM_033031
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTACTGCCACTACCACCCCAGAGCTCCAAACCTGTGCCTAAGAAATCTCAGTCCAGCAAAATTGTGC CCAGTCATCATGACCCATCTGAAAAGACGGGGGAGAATTGCCAAACGAAGATATCTCCATCTTCACTTCA GGAGTCTCCATCTTCACTTCAGGGAGCACTCAAAAAGAGATCAGCTTTTGAAGATCTCACTAATGCTTCT CAATGTCAACCTGTCCAGCCCAAGAAAGAAGCCAATAAAGAGTTTGTAAAAGTTGTTTCCAAGAAGATAA ACAGGAACACACATGCTCTTGGACTGGCCAAAAAGAATAAGCGGAATCTAAAATGGCATAAGCTGGAAGT CACACCAGTAGTAGCCTCTACTACCGTGGTACCAAACATTATGGAGAAACCACTCATTCTAGACATATCC ACCACCTCCAAAACACCCAACACTGAGGAGGCATCTCTCTTCAGAAAGCCATTAGTTTTAAAGGAGGAAC CCACTATTGAGGATGAAACCCTTATCAATAAGTCATTATCTTTAAAAAAGTGCTCAAATCATGAGGAGGT GTCCTTACTGGAAAAGCTACAGCCCCTGCAGGAGGAGAGTGACAGTGATGATGCGTTTGTTATAGAGCCA ATGACTTTTAAGAAGACACATAAAACTGAGGAGGCAGCCATCACCAAGAAGACATTATCCTTAAAGAAGA AGATGTGTGCAAGTCAGCGGAAGCAGTCCTGCCAGGAAGAGTCGTTGGCTGTGCAGGATGTCAATATGGA AGAGGATTCCTTCTTTATGGAGTCAATGAGTTTTAAGAAGAAGCCTAAAACTGAGGAGTCAATCCCCACC CATAAGTTATCATCTTTAAAGAAGAAATGTACCATTTATGGGAGGATATGCCACTTTAGGAAGCCACCAG TATTGCAGACAACCATCTGTGGAGCAATGTCCTCCATTAAGAAGCCTACCACTGAGAAGGAGACACTTTT CCAAGAGCTATCTGTATTGCAAGAGAAACACACCACTGAGCATGAGATGTCCATCTTGAAGAAATCATTG GCCTTGCAGAAGACCAACTTTAAAGAGGATTCCCTTGTTAAGGAGTCGTTAGCCTTTAAGAAGAAGCCTA GCACTGAGGAGGCAATCATGATGCCAGTAATATTGAAGGAGCAGTGCATGACTGAGGGGAAGAGGCCCCG TCTGAAGCCATTAGTATTGCAGGAGATCACCTCTGGAGAGAAGTCGCTCATTATGAAGCCATTGTCCATT AAAGAAAAGCCATCTACTGAGAAGGAGTCCTTTTCCCAGGAACCATCTGCATTGCAAAAGAAGCACACCA CTCAGGAGGAGGTTTCCATCTTAAAGGAGCCCTCGTCCTTGCTAAAGTCTCCAACTGAGGAGTCACCTTT TGATGAGGCTTTGGCTTTTACAAAGAAGTGTACCATTGAGGAGGCACCCCCCACCAAGAAGCCTTTAATT TTAAAGAAGAAGCATGCCACTCAGGGGACAATGTCCCACTTGAAGAAACCACTAATATTACAGACCACCT CTGGAGAAAAGTCACTTATTAAGGAGCCACTGCCCTTTAAAGAAGAAAAAGTGTCTTTAAAGAAAAAGTG TACCACACAAGAGATGATGTCCATCTGTCCAGAACTGTTGGACTTTCAGGATATGATTGGTGAAGATAAG AATTCCTTCTTTATGGAGCCAATGTCATTTAGGAAGAGCCCTACAACTGAGGAGACAGTACTTACCAAGA CATCGTTGTCTTTACAGGAAAAGAAAATTACTCAGGGGGAGATGTCCCACTTAAAGAAGCCACTGGTCTT GCAGAAGATCACTTCTGAGGAGGAGTCATTCTATAAGAAGCTGTTGCCCTTTAAGATGAAATCTACAACG GAAGAAAAGTTCCTCTCCCAGGAACCATCTGCATTGAAAGAGAAGCATACCACCTTGCAGGAAGTGTCCC TCTCAAAAGAGTCATTGGCCATCCAAGAGAAGGCTACCACTGAGGAGGAATTCTCTCAGGAACTATTTTC ATTGCATGTTAAGCATACCAACAAAAGTGGGTCCCTCTTCCAGGAGGCTTTGGTCTTGCAAGAGAAGACT GATGCCGAAGAGGATTCCTTGAAGAACTTGTTGGCTTTGCAGGAGAAAAGCACCATGGAAGAAGAGTCCC TTATCAATAAGCTATTGGCTCTGAAGGAGGAGCTTTCTGCTGAGGCAGCCACAAACATACAGACACAATT ATCTTTAAAGAAGAAGTCCACTTCTCATGGAAAAGTGTTCTTCCTGAAGAAGCAGTTGGCTTTGAATGAG ACCATCAATGAAGAGGAGTTCCTTAATAAGCAGCCACTGGCCTTGGAGGGGTATCCCAGCATTGCGGAGG GGGAGACCCTCTTCAAGAAGCTTTTGGCCATGCAGGAGGAGCCCAGCATTGAGAAGGAAGCTGTCCTCAA GGAGCCCACTATTGACACAGAAGCTCACTTTAAGGAACCTTTGGCCTTGCAGGAGGAGCCCAGCACTGAG AAGGAGGCTGTCCTCAAGGAGCCCAGTGTTGACACAGAAGCTCACTTTAAGGAAACTTTGGCCTTGCAGG AGAAGCCCAGCATTGAGCAGGAGGCCCTCTTTAAGCGACACTCAGCTTTGTGGGAGAAGCCCAGCACTGA GAAGGAGACCATCTTCAAGGAGTCTTTGGACTTGCAAGAGAAGCCCAGCATTAAGAAAGAGACCCTCCTC AAAAAGCCATTAGCCTTGAAGATGTCTACCATCAATGAGGCAGTCCTCTTCGAAGATATGATAGCTCTGA ATGAGAAACCCACCACTGGGAAGGAGTTGTCCTTCAAGGAGCCATTAGCCTTACAAGAGAGTCCCACCTA CAAGGAAGACACCTTTCTCAAAACATTGTTGGTCCCCCAAGTTGGAACCAGCCCAAATGTGTCTAGCACT GCCCCTGAATCCATAACCAGCAAGTCCAGCATTGCTACCATGACCAGTGTGGGCAAATCTGGTACCATCA ATGAGGCATTCCTCTTCGAAGATATGATAACTCTGAATGAGAAACCCACCACTGGGAAGGAGTTGTCCTT CAAGGAGCCATTGGCCTTACAAGAGAGTCCCACCTGCAAGGAAGACACCTTTCTGGAAACATTCTTGATC CCCCAAATTGGAACCAGCCCATATGTGTTTAGCACCACCCCTGAATCCATAACAGAGAAGTCCAGCATTG CAACCATGACCAGCGTGGGCAAGTCCAGGACCACCACCGAGTCCAGTGCATGTGAATCTGCTTCTGATAA ACCTGTCTCACCACAGGCCAAGGGAACACCAAAGGAGATAACCCCACGGGAAGATATTGATGAGGACAGC AGTGATCCAAGTTTCAACCCAATGTATGCCAAGGAAATCTTCAGTTACATGAAAGAGAGAGAGGAACAGT TTATACTTACAGATTACATGAACAGGCAGATTGAAATCACCAGTGACATGAGGGCCATTCTTGTGGACTG GTTGGTGGAGGTGCAGGTGTCCTTTGAGATGACCCATGAGACCCTGTACTTGGCAGTGAAGCTGGTGGAT CTCTACCTAATGAAGGCAGTATGCAAGAAGGATAAGTTACAACTCCTTGGTGCCACTGCCTTTATGATTG CAGCAAAATTTGAGGAGCACAACTCACCTCGTGTGGATGACTTTGTGTACATCTGTGATGATAATTATCA GCGATCTGAGGTACTCAGCATGGAAATCAACATCCTGAACGTCCTCAAATGTGACATTAACATTCCCATC GCCTACCATTTTCTGCGCAGATATGCTAGGTGTATCCACACCAACATGAAGACACTGACCTTGTCCCGCT ACATCTGCGAGATGACCCTGCAGGAATACCACTATGTCCAGGAGAAGGCTTCCAAGCTAGCTGCTGCCTC CTTACTCCTGGCCCTCTACATGAAGAAGCTCGGATACTGGGTTCCCTTCCTGGAGCATTACAGTGGCTAC AGTATCTCTGAGCTTCACCCCTTGGTCAGACAGCTGAACAAACTGCTGACTTTCAGTTCTTACGATAGTC TCAAGGCTGTGTATTACAAGTATTCTCACCCGGTCTTCTTTGAAGTCGCCAAAATCCCTGCCTTGGATAT GTTGAAGCTGGAGGAGATTTTGAACTGTGATTGTGAGGCTCAGGGCCTGGTACTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217591 representing NM_033031
Red=Cloning site Green=Tags(s) MLLPLPPQSSKPVPKKSQSSKIVPSHHDPSEKTGENCQTKISPSSLQESPSSLQGALKKRSAFEDLTNAS QCQPVQPKKEANKEFVKVVSKKINRNTHALGLAKKNKRNLKWHKLEVTPVVASTTVVPNIMEKPLILDIS TTSKTPNTEEASLFRKPLVLKEEPTIEDETLINKSLSLKKCSNHEEVSLLEKLQPLQEESDSDDAFVIEP MTFKKTHKTEEAAITKKTLSLKKKMCASQRKQSCQEESLAVQDVNMEEDSFFMESMSFKKKPKTEESIPT HKLSSLKKKCTIYGRICHFRKPPVLQTTICGAMSSIKKPTTEKETLFQELSVLQEKHTTEHEMSILKKSL ALQKTNFKEDSLVKESLAFKKKPSTEEAIMMPVILKEQCMTEGKRPRLKPLVLQEITSGEKSLIMKPLSI KEKPSTEKESFSQEPSALQKKHTTQEEVSILKEPSSLLKSPTEESPFDEALAFTKKCTIEEAPPTKKPLI LKKKHATQGTMSHLKKPLILQTTSGEKSLIKEPLPFKEEKVSLKKKCTTQEMMSICPELLDFQDMIGEDK NSFFMEPMSFRKSPTTEETVLTKTSLSLQEKKITQGEMSHLKKPLVLQKITSEEESFYKKLLPFKMKSTT EEKFLSQEPSALKEKHTTLQEVSLSKESLAIQEKATTEEEFSQELFSLHVKHTNKSGSLFQEALVLQEKT DAEEDSLKNLLALQEKSTMEEESLINKLLALKEELSAEAATNIQTQLSLKKKSTSHGKVFFLKKQLALNE TINEEEFLNKQPLALEGYPSIAEGETLFKKLLAMQEEPSIEKEAVLKEPTIDTEAHFKEPLALQEEPSTE KEAVLKEPSVDTEAHFKETLALQEKPSIEQEALFKRHSALWEKPSTEKETIFKESLDLQEKPSIKKETLL KKPLALKMSTINEAVLFEDMIALNEKPTTGKELSFKEPLALQESPTYKEDTFLKTLLVPQVGTSPNVSST APESITSKSSIATMTSVGKSGTINEAFLFEDMITLNEKPTTGKELSFKEPLALQESPTCKEDTFLETFLI PQIGTSPYVFSTTPESITEKSSIATMTSVGKSRTTTESSACESASDKPVSPQAKGTPKEITPREDIDEDS SDPSFNPMYAKEIFSYMKEREEQFILTDYMNRQIEITSDMRAILVDWLVEVQVSFEMTHETLYLAVKLVD LYLMKAVCKKDKLQLLGATAFMIAAKFEEHNSPRVDDFVYICDDNYQRSEVLSMEINILNVLKCDINIPI AYHFLRRYARCIHTNMKTLTLSRYICEMTLQEYHYVQEKASKLAAASLLLALYMKKLGYWVPFLEHYSGY SISELHPLVRQLNKLLTFSSYDSLKAVYYKYSHPVFFEVAKIPALDMLKLEEILNCDCEAQGLVL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033031 |
| ORF Size | 4185 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_033031.1, NP_149020.1 |
| RefSeq Size | 4513 bp |
| RefSeq ORF | 4188 bp |
| Locus ID | 85417 |
| UniProt ID | Q8WWL7 |
| Protein Families | Druggable Genome |
| Protein Pathways | Cell cycle, p53 signaling pathway, Progesterone-mediated oocyte maturation |
| Gene Summary | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as positive regulators of cyclin-dependent kinases (CDKs), and thereby play an essential role in the control of the cell cycle. Different cyclins exhibit distinct expression and degradation patterns, which contribute to the temporal coordination of each mitotic event. Studies of similar genes in chicken and drosophila suggest that this cyclin may associate with CDC2 and CDK2 kinases, and may be required for proper spindle reorganization and restoration of the interphase nucleus. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217591 | CCNB3 (Myc-DDK-tagged)-Human cyclin B3 (CCNB3), transcript variant 3 |
CNY 9,544.00 |
|
| RC217591L3 | Lenti-ORF clone of CCNB3 (Myc-DDK-tagged)-Human cyclin B3 (CCNB3), transcript variant 3 |
CNY 15,770.00 |
|
| RC217591L4 | Lenti-ORF clone of CCNB3 (mGFP-tagged)-Human cyclin B3 (CCNB3), transcript variant 3 |
CNY 15,770.00 |
|
| SC305536 | CCNB3 (untagged)-Human cyclin B3 (CCNB3), transcript variant 3 |
CNY 26,890.00 |
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