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TAF9B (NM_015975) Human Tagged ORF Clone
CAT＃: RG217413
- TrueORF®
TAF9B (tGFP-tagged) - Human TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa (TAF9B)
ORF Plasmid: DDK
"NM_015975" in other vectors (4)
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CNY 4,000.00
CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DN-7; DN7; TAF9L; TAFII31L; TFIID-31 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG217413 representing NM_015975
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCGGGCAAGATGGCGCCTCCCAAGAACGCTCCGAGAGATGCCTTGGTGATGGCACAGATCCTGA AGGATATGGGAATCACAGAGTATGAACCAAGGGTTATAAATCAAATGTTGGAATTTGCTTTCCGTTATGT GACTACAATTCTGGATGATGCAAAAATTTATTCGAGCCATGCTAAGAAACCTAATGTTGATGCAGATGAT GTGAGACTGGCAATCCAGTGTCGTGCTGACCAATCTTTTACCTCTCCTCCCCCAAGAGATTTTTTACTGG ATATCGCAAGGCAGAAAAATCAAACCCCTTTGCCACTGATTAAGCCATATGCAGGACCTAGACTGCCACC TGATAGATACTGCTTAACAGCTCCAAACTATAGGCTGAAGTCCTTAATTAAAAAGGGACCTAACCAAGGG AGACTAGTTCCACGATTAAGTGTTGGTGCTGTTAGTAGCAAACCTACTACTCCTACTATAGCAACCCCAC AAACGGTGTCTGTCCCAAATAAAGTTGCAACTCCAATGTCAGTGACAAGCCAAAGATTTACGGTGCAGAT TCCACCTTCTCAGTCCACACCTGTCAAACCAGTTCCTGCAACAACTGCAGTTCAAAATGTTCTGATTAAT CCTTCAATGATTGGGCCCAAAAATATTCTTATTACCACCAACATGGTTTCGTCACAGAACACAGCCAATG AAGCAAACCCACTGAAGAGAAAACATGAAGATGATGATGACAATGATATTATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217413 representing NM_015975
Red=Cloning site Green=Tags(s) MESGKMAPPKNAPRDALVMAQILKDMGITEYEPRVINQMLEFAFRYVTTILDDAKIYSSHAKKPNVDADD VRLAIQCRADQSFTSPPPRDFLLDIARQKNQTPLPLIKPYAGPRLPPDRYCLTAPNYRLKSLIKKGPNQG RLVPRLSVGAVSSKPTTPTIATPQTVSVPNKVATPMSVTSQRFTVQIPPSQSTPVKPVPATTAVQNVLIN PSMIGPKNILITTNMVSSQNTANEANPLKRKHEDDDDNDIM TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015975 |
| ORF Size | 753 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015975.5 |
| RefSeq Size | 2734 bp |
| RefSeq ORF | 756 bp |
| Locus ID | 51616 |
| UniProt ID | Q9HBM6 |
| Domains | TFIID-31 |
| Protein Families | Transcription Factors |
| Protein Pathways | Basal transcription factors |
| Gene Summary | Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a protein that is similar to one of the small subunits of TFIID, TBP-associated factor 9, and is also a subunit of TFIID. TAF9 and TAF9b share some functions but also have distinct roles in the transcriptional regulatory process. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217413 | TAF9B (Myc-DDK-tagged)-Human TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa (TAF9B) |
CNY 2,400.00 |
|
| RC217413L3 | Lenti ORF clone of Human TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa (TAF9B), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC217413L4 | Lenti ORF clone of Human TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa (TAF9B), mGFP tagged |
CNY 5,890.00 |
|
| SC114555 | TAF9B (untagged)-Human TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa (TAF9B) |
CNY 2,400.00 |
