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PCDHGA11 (NM_018914) Human Tagged ORF Clone
CAT＃: RG217347
- TrueORF®
PCDHGA11 (tGFP-tagged) - Human protocadherin gamma subfamily A, 11 (PCDHGA11), transcript variant 1
ORF Plasmid: DDK
"NM_018914" in other vectors (4)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PCDH-GAMMA-A11 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG217347 representing NM_018914
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAATCGGCTACAGCGCGGGGACCGCAGTCGGCTGCTGCTGCTGCTGTGCATTTTCCTGGGGACGC TGCGGGGGTTCCGGGCCAGGCAGATCCGATATTCGGTGCCAGAAGAGACCGAAAAGGGCTCCTTCGTGGG CAATATCTCCAAGGACCTGGGGCTGGAGCCCCGGGAGCTGGCGAAGCGCGGAGTCCGCATCGTCTCCAGA GGGAAGACACAGCTTTTCGCTGTGAATCCGCGAAGCGGCAGCTTGATCACGGCAGGCAGGATAGACCGGG AGGAGCTCTGTGAGACGGTGTCCTCCTGTTTTTTAAATATGGAACTTCTCGTGGAAGACACCTTGAAGAT TTACGGAGTGGAGGTGGAAATAATAGATATTAATGATAACGCCCCCAGCTTCCAGGAGGACGAAGTGGAG ATAAAAGTCAGTGAGCACGCAATTCCTGGGGCGCGATTTGCTCTTCCTAATGCTAGGGATCCAGATGTGG GCGTGAACTCCCTCCAGAGCTACCAGCTCAGCCCTAATAATTACTTTTCCTTGCAACTGCGGGGCAGAAC GGATGGGGCCAAGAATCCAGAGCTAGTACTGGAGGGAAGCCTGGACCGAGAGAAAGAGGCTGCTCACCTG CTCCTCCTCACAGCTTTAGATGGAGGCGATCCCATCCGAAAGGGCGCAGTTCCCATTCGTGTGGTGGTCC TCGATGTAAATGATCACATCCCAATGTTTACACAGTCCGTATATCGCGTGAGTGTTCCAGAAAACATCAG CTCCGGAACTCGGGTGCTGATGGTTAATGCAACGGATCCAGACGAGGGAATCAACGGGGAAGTAATGTAT TCATTTCGGAACATGGAAAGCAAGGCTTCTGAAATATTCCAATTGGATTCACAAACTGGAGAAGTTCAAG TACGGGGGTCTCTGGATTTTGAAAAATATAGATTCTATGAGATGGAAATTCAAGGCCAAGATGGTGGAGG TCTCTTTACCACCACGACGATGTTGATCACTGTTGTGGATGTGAATGATAACGCTCCAGAAATAACTATC ACCTCTTCTATTAATTCAATTCTGGAAAACTCTCCTCCAGGTACAGTGATTGCTCTTCTAAATGTGCAAG ATCAAGATTCTGGAGAAAATGGTCAAGTCTCCTGTTTTATTCCTAACCACCTGCCTTTTAAATTAGAAAA GACTTATGGAAATTATTACAAATTGATAACAAGCAGAGTGCTGGACAGGGAGTTGGTCCAGAGCTACAAT ATAACGTTGACAGCCACAGACCAGGGAAGCCCGCCTTTGTCTGCAGAAACTCATGTCTGGCTGAATGTGG CAGATGACAACGATAACCCTCCCGTTTTTCCTCACTCCTCTTACTCTGCCTACATTCCCGAAAACAACCC CAGGGGTGCCTCCATCTTCTCAGTGACCGCCCTCGACCCGGACAGCAAACAGAATGCCCTGGTCACTTAC TCTCTGACGGATGACACTGTCCAGGGGGTGCCTCTGTCCTCCTATGTCTCTATTAACTCCAACACTGGTG TTCTCTATGCCCTACAATCCTTCGACTATGAGCAGTTTCGAGACTTAGAACTGAGAGTGATAGCACGTGA CAGCGGGGACCCGCCCCTCAGCAGCAACGTGTCGCTGAGCCTGTTCGTGCTGGACCAGAACGACAATGCG CCCGAGATCCTGTACCCTGCCCTCCCCACAGACGGCTCCACTGGCGTGGAGCTGGCGCCCCGCTCTGCGG AACCTGGCTACCTGGTGACCAAGGTGGTTGCGGTGGACAAAGATTCAGGCCAGAACGCCTGGCTGTCCTA TCGCCTGCTTAAGGCCAGCGAGCCGGGACTCTTCGCGGTGGGGGAGCACACGGGCGAGGTGCGTACAGCG CGGGCACTGCTGGACAGAGACGCGCTCAAGCAGAGCCTCGTGGTGGCCGTCCAGGACCACGGCCAGCCCC CTCTCTCGGCCACCGTCACGCTCACCGTGGCTGTGGCCGACAGCATCCCCGAAGTCCTGGCGGACCTCGG CAGCCTCGAGTCTCTGGCTAACTCTGAAACCTCAGACCTCTCGCTGTACTTGGTGGTGGCGGTGGCCGCA GTCTCCTGCATCTTCCTGGTCTTTGTCATCGTGCTGCTGGCACTCAGGCTGTGGCGCTGGCATAAGTCAC GCCTGCTGCAGGCTTCTGAAGGCGGGTTGGCAGGTATGCCCACGTCACATTTTGTAGGCGTGGACGGGGT ACAGGCTTTCCTGCAAACCTATTCCCACGAGGTCTCTCTCATTGCGGACTCGCAGAAGAGTCACCTGATT TTCCCCCAGCCCAACTATGGGGACACGCTCATCAGCCAGGAGAGCTGTGAGAAAAGCGAGCCACTCTTGA TAGCTGAAGACTCAGCTATCATTTTAGGCAAATGTGACCCGACAAGTAATCAGCAAGCCCCGCCCAACAC GGACTGGCGTTTCTCTCAGGCCCAGAGACCCGGCACCAGCGGCTCCCAAAATGGCGATGACACCGGCACC TGGCCCAACAACCAGTTTGACACAGAGATGCTGCAAGCCATGATCTTGGCGTCCGCCAGTGAAGCTGCTG ATGGGAGCTCCACCCTGGGAGGGGGTGCCGGCACCATGGGATTGAGCGCCCGCTACGGACCCCAGTTCAC CCTGCAGCACGTGCCCGACTACCGCCAGAATGTCTACATCCCAGGCAGCAATGCCACACTGACCAACGCA GCTGGCAAGCGGGATGGCAAGGCCCCAGCAGGTGGCAATGGCAACAAGAAGAAGTCGGGCAAGAAGGAGA AGAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217347 representing NM_018914
Red=Cloning site Green=Tags(s) MANRLQRGDRSRLLLLLCIFLGTLRGFRARQIRYSVPEETEKGSFVGNISKDLGLEPRELAKRGVRIVSR GKTQLFAVNPRSGSLITAGRIDREELCETVSSCFLNMELLVEDTLKIYGVEVEIIDINDNAPSFQEDEVE IKVSEHAIPGARFALPNARDPDVGVNSLQSYQLSPNNYFSLQLRGRTDGAKNPELVLEGSLDREKEAAHL LLLTALDGGDPIRKGAVPIRVVVLDVNDHIPMFTQSVYRVSVPENISSGTRVLMVNATDPDEGINGEVMY SFRNMESKASEIFQLDSQTGEVQVRGSLDFEKYRFYEMEIQGQDGGGLFTTTTMLITVVDVNDNAPEITI TSSINSILENSPPGTVIALLNVQDQDSGENGQVSCFIPNHLPFKLEKTYGNYYKLITSRVLDRELVQSYN ITLTATDQGSPPLSAETHVWLNVADDNDNPPVFPHSSYSAYIPENNPRGASIFSVTALDPDSKQNALVTY SLTDDTVQGVPLSSYVSINSNTGVLYALQSFDYEQFRDLELRVIARDSGDPPLSSNVSLSLFVLDQNDNA PEILYPALPTDGSTGVELAPRSAEPGYLVTKVVAVDKDSGQNAWLSYRLLKASEPGLFAVGEHTGEVRTA RALLDRDALKQSLVVAVQDHGQPPLSATVTLTVAVADSIPEVLADLGSLESLANSETSDLSLYLVVAVAA VSCIFLVFVIVLLALRLWRWHKSRLLQASEGGLAGMPTSHFVGVDGVQAFLQTYSHEVSLIADSQKSHLI FPQPNYGDTLISQESCEKSEPLLIAEDSAIILGKCDPTSNQQAPPNTDWRFSQAQRPGTSGSQNGDDTGT WPNNQFDTEMLQAMILASASEAADGSSTLGGGAGTMGLSARYGPQFTLQHVPDYRQNVYIPGSNATLTNA AGKRDGKAPAGGNGNKKKSGKKEKK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018914 |
| ORF Size | 2805 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018914.3 |
| RefSeq Size | 4872 bp |
| RefSeq ORF | 2808 bp |
| Locus ID | 56105 |
| UniProt ID | Q9Y5H2 |
| Domains | CA |
| Protein Families | Transmembrane |
| Gene Summary | This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217347 | PCDHGA11 (Myc-DDK-tagged)-Human protocadherin gamma subfamily A, 11 (PCDHGA11), transcript variant 1 |
CNY 6,848.00 |
|
| RC217347L3 | Lenti-ORF clone of PCDHGA11 (Myc-DDK-tagged)-Human protocadherin gamma subfamily A, 11 (PCDHGA11), transcript variant 1 |
CNY 9,030.00 |
|
| RC217347L4 | Lenti-ORF clone of PCDHGA11 (mGFP-tagged)-Human protocadherin gamma subfamily A, 11 (PCDHGA11), transcript variant 1 |
CNY 9,030.00 |
|
| SC309669 | PCDHGA11 (untagged)-Human protocadherin gamma subfamily A, 11 (PCDHGA11), transcript variant 1 |
CNY 7,220.00 |
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