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RhoGAP (ARHGAP5) (NM_001173) Human Tagged ORF Clone
CAT＃: RG217131
- TrueORF®
ARHGAP5 (tGFP-tagged) - Human Rho GTPase activating protein 5 (ARHGAP5), transcript variant 2
ORF Plasmid: DDK
"NM_001173" in other vectors (4)
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CNY 11,872.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GFI2; p190-B; p190BRhoGAP; RhoGAP5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG217131 representing NM_001173
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGCAAAAAACAAAGAGCCTCGTCCCCCATCCTATACCATCAGTATAGTTGGACTCTCTGGGACTG AAAAAGACAAAGGTAACTGTGGAGTTGGAAAGTCTTGTTTGTGCAATAGATTTGTACGCTCAAAAGCAGA TGAATATTATCCAGAGCATACTTCTGTGCTTAGCACCATTGACTTTGGAGGACGAGTAGTAAACAATGAT CACTTTTTGTACTGGGGTGACATAATACAAAATAGTGAAGATGGAGTAGAATGCAAAATTCATGTCATTG AACAAACAGAGTTCATTGATGACCAGACTTTCTTGCCTCATCGGAGTACGAATTTGCAACCATATATAAA ACGTGCAGCTGCATCTAAATTGCAGTCAGCAGAAAAACTAATGTACATTTGCACTGATCAGCTAGGCTTA GAACAAGACTTTGAACAGAAGCAAATGCCTGAAGGGAAGCTCAACGTAGATGGATTTTTATTATGCATTG ATGTAAGTCAAGGATGCAATAGGAAGTTTGATGATCAACTTAAATTTGTGAATAACCTTTTTGTCCAGTT ATCAAAATCAAAAAAACCTGTAATAATAGCAGCAACTAAATGTGATGAATGCGTGGATCATTATCTTAGA GAAGTTCAGGCATTTGCTTCAAATAAAAAGAACCTTCTTGTAGTGGAAACATCAGCACGATTTAATGTCA ACATTGAAACATGTTTTACTGCACTGGTACAAATGTTGGATAAAACTCGTAGCAAGCCTAAAATTATTCC CTATTTGGATGCTTATAAAACACAGAGACAACTTGTTGTCACAGCAACAGATAAGTTTGAAAAACTTGTG CAGACTGTGAGAGATTATCATGCAACTTGGAAAACTGTTAGTAATAAATTAAAAAATCATCCTGATTATG AAGAATACATCAACTTAGAGGGAACAAGAAAGGCCAGAAATACATTCTCAAAACATATAGAACAACTTAA ACAGGAACATATAAGAAAAAGGAGAGAAGAGTATATAAATACTTTACCAAGAGCTTTTAACACTCTTTTG CCAAATCTAGAAGAGATTGAACATTTGAATTGGTCAGAAGCTTTGAAGTTAATGGAAAAGAGAGCAGATT TCCAGTTATGTTTTGTGGTGCTAGAAAAAACTCCTTGGGATGAAACTGACCATATAGACAAAATTAATGA TAGGCGGATTCCATTTGACCTCCTGAGCACTTTAGAAGCTGAAAAAGTCTATCAGAACCATGTACAGCAT CTGATATCCGAGAAGAGGAGGGTGGAAATGAAGGAAAAATTCAAAAAGACTTTGGAAAAAATTCAATTCA TTTCACCAGGGCAGCCATGGGAGGAAGTTATGTGCTTTGTTATGGAGGATGAAGCCTACAAATATATCAC TGAGGCTGATAGCAAAGAGGTATATGGTAGGCATCAGCGAGAAATAGTTGAAAAAGCCAAAGAAGAGTTT CAAGAAATGCTTTTTGAGCATTCTGAACTTTTTTATGATTTAGATCTTAATGCAACACCTAGTTCAGATA AAATGAGTGAAATTCATACAGTTCTGAGTGAAGAACCTAGATATAAAGCTTTACAGAAACTTGCACCTGA TAGGGAATCCCTTCTACTTAAGCATATAGGATTTGTTTATCATCCCACTAAAGAAACATGTCTTAGTGGC CAAAATTGTACAGACATTAAAGTGGAGCAGTTACTTGCTAGTAGTCTTTTACAGTTGGATCATGGCCGCT TAAGATTATATCACGATAGTACCAATATAGATAAAGTTAACCTTTTTATTTTAGGGAAGGATGGCCTTGC CCAAGAACTAGCAAATGAGATAAGGACACAATCCACTGATGATGAGTATGCCTTAGATGGAAAAATTTAT GAACTTGATCTTCGGCCGGTTGATGCCAAATCGCCTTACTTTTTGAGTCAGTTATGGACTGCCGCCTTTA AACCACATGGGTGCTTCTGTGTATTTAATTCCATTGAGTCATTGAGTTTTATTGGGGAATTTATTGGGAA AATAAGAACTGAAGCTTCTCAGATCAGAAAAGATAAATACATGGCTAATCTTCCATTTACATTAATTCTG GCTAATCAGAGAGATTCCATTAGTAAGAATCTACCAATTCTCAGGCACCAAGGGCAGCAGTTGGCAAACA AGTTGCAATGTCCTTTTGTAGATGTACCTGCTGGTACATATCCTCGTAAATTTAATGAAACCCAAATAAA GCAAGCTCTCAGAGGAGTATTGGAATCAGTTAAACACAATTTGGATGTGGTGAGCCCAATTCCTGCCAAT AAGGACTTATCAGAAGCTGACTTGAGAATTGTCATGTGCGCCATGTGTGGAGATCCATTTAGTGTGGATC TTATTCTTTCACCCTTCCTTGATTCTCATTCTTGCAGTGCTGCTCAAGCTGGACAGAATAATTCCCTAAT GCTTGATAAAATCATTGGTGAAAAAAGGAGGCGAATACAGATCACAATATTATCATACCACTCTTCAATT GGAGTAAGAAAAGATGAACTAGTTCATGGGTATATATTAGTTTACTCTGCAAAACGGAAAGCTTCGATGG GAATGCTTCGAGCATTTCTATCAGAAGTTCAAGACACCATTCCTGTACAGCTGGTGGCAGTTACTGACAG CCAAGCAGATTTTTTTGAAAATGAGGCTATCAAAGAGTTAATGACTGAAGGAGAACACATTGCAACTGAG ATCACTGCTAAATTTACAGCACTGTATTCTTTATCTCAGTATCATCGGCAAACTGAGGTCTTTACTCTGT TTTTTAGTGATGTTCTAGAGAAAAAAAATATGATAGAAAATTCTTATTTGTCTGATAATACAAGGGAATC AACCCATCAAAGTGAAGATGTTTTTCTACCATCTCCCAGAGACTGTTTTCCCTATAATAACTACCCTGAT TCAGATGATGACACAGAAGCACCACCTCCTTATAGTCCAATTGGGGATGATGTACAGTTGCTTCCAACAC CTAGTGACCGTTCCAGATATAGATTAGATTTGGAAGGAAATGAGTATCCTATTCATAGTACCCCAAACTG TCATGACCATGAACGCAACCATAAAGTGCCTCCACCTATTAAACCTAAACCAGTTGTACCTAAGACAAAT GTGAAAAAACTCGATCCAAACCTTTTAAAAACAATTGAAGCTGGTATTGGTAAAAATCCAAGAAAGCAGA CTTCCCGGGTGCCTTTGGCACATCCTGAAGATATGGATCCTTCAGATAACTATGCGGAACCCATTGATAC AATTTTCAAACAGAAGGGCTATTCTGATGAGATTTATGTTGTCCCAGATGATAGTCAAAATCGTATTAAA ATTCGAAACTCATTTGTAAATAACACCCAAGGAGATGAAGAAAATGGGTTTTCTGATAGAACCTCAAAAA GTCATGGGGAACGGAGGCCTTCAAAATACAAATATAAATCTAAAACCTTGTTTAGTAAAGCCAAGTCATA CTATAGAAGAACACATTCAGATGCCAGTGATGATGAGGCTTTCACCACTTCTAAAACAAAAAGAAAAGGA AGACATCGTGGAAGTGAAGAAGATCCACTTCTTTCTCCTGTTGAAACTTGGAAAGGTGGTATTGATAATC CTGCAATCACTTCTGACCAGGAGTTAGATGATAAGAAGATGAAGAAGAAAACCCACAAAGTGAAAGAAGA TAAAAAGAAAAAGAAAACTAAGAACTTCAATCCACCAACACGTAGAAATTGGGAAAGTAATTACTTTGGG ATGCCCCTCCAGGATCTGGTTACAGCTGAGAAGCCCATACCACTATTTGTTGAGAAATGTGTGGAATTTA TTGAAGATACAGGGTTATGTACCGAAGGACTCTACCGTGTCAGCGGGAATAAAACTGACCAAGACAATAT TCAAAAGCAGTTTGATCAAGATCATAATATCAATCTAGTGTCAATGGAAGTAACAGTAAATGCTGTAGCT GGAGCCCTTAAAGCTTTCTTTGCAGATCTGCCAGATCCTTTAATTCCATATTCTCTTCATCCAGAACTAT TGGAAGCAGCAAAAATCCCGGATAAAACAGAACGTCTTCATGCCTTGAAAGAAATTGTTAAGAAATTTCA TCCTGTAAACTATGATGTATTCAGATACGTGATAACACATCTAAACAGGGTTAGTCAGCAACATAAAATC AACCTAATGACAGCAGACAACTTATCCATCTGTTTTTGGCCAACCTTGATGAGACCTGATTTTGAAAATC GAGAGTTTCTGTCTACTACTAAGATTCATCAATCTGTTGTTGAAACATTCATTCAGCAGTGTCAGTTTTT CTTTTACAATGGAGAAATTGTAGAAACGACAAACATTGTGGCTCCTCCACCACCTTCAAACCCAGGACAG TTGGTGGAACCAATGGTGCCACTTCAGTTGCCGCCACCATTGCAACCTCAGCTGATACAACCACAATTAC AAACGGATCCTCTTGGTATTATA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217131 representing NM_001173
Red=Cloning site Green=Tags(s) MMAKNKEPRPPSYTISIVGLSGTEKDKGNCGVGKSCLCNRFVRSKADEYYPEHTSVLSTIDFGGRVVNND HFLYWGDIIQNSEDGVECKIHVIEQTEFIDDQTFLPHRSTNLQPYIKRAAASKLQSAEKLMYICTDQLGL EQDFEQKQMPEGKLNVDGFLLCIDVSQGCNRKFDDQLKFVNNLFVQLSKSKKPVIIAATKCDECVDHYLR EVQAFASNKKNLLVVETSARFNVNIETCFTALVQMLDKTRSKPKIIPYLDAYKTQRQLVVTATDKFEKLV QTVRDYHATWKTVSNKLKNHPDYEEYINLEGTRKARNTFSKHIEQLKQEHIRKRREEYINTLPRAFNTLL PNLEEIEHLNWSEALKLMEKRADFQLCFVVLEKTPWDETDHIDKINDRRIPFDLLSTLEAEKVYQNHVQH LISEKRRVEMKEKFKKTLEKIQFISPGQPWEEVMCFVMEDEAYKYITEADSKEVYGRHQREIVEKAKEEF QEMLFEHSELFYDLDLNATPSSDKMSEIHTVLSEEPRYKALQKLAPDRESLLLKHIGFVYHPTKETCLSG QNCTDIKVEQLLASSLLQLDHGRLRLYHDSTNIDKVNLFILGKDGLAQELANEIRTQSTDDEYALDGKIY ELDLRPVDAKSPYFLSQLWTAAFKPHGCFCVFNSIESLSFIGEFIGKIRTEASQIRKDKYMANLPFTLIL ANQRDSISKNLPILRHQGQQLANKLQCPFVDVPAGTYPRKFNETQIKQALRGVLESVKHNLDVVSPIPAN KDLSEADLRIVMCAMCGDPFSVDLILSPFLDSHSCSAAQAGQNNSLMLDKIIGEKRRRIQITILSYHSSI GVRKDELVHGYILVYSAKRKASMGMLRAFLSEVQDTIPVQLVAVTDSQADFFENEAIKELMTEGEHIATE ITAKFTALYSLSQYHRQTEVFTLFFSDVLEKKNMIENSYLSDNTRESTHQSEDVFLPSPRDCFPYNNYPD SDDDTEAPPPYSPIGDDVQLLPTPSDRSRYRLDLEGNEYPIHSTPNCHDHERNHKVPPPIKPKPVVPKTN VKKLDPNLLKTIEAGIGKNPRKQTSRVPLAHPEDMDPSDNYAEPIDTIFKQKGYSDEIYVVPDDSQNRIK IRNSFVNNTQGDEENGFSDRTSKSHGERRPSKYKYKSKTLFSKAKSYYRRTHSDASDDEAFTTSKTKRKG RHRGSEEDPLLSPVETWKGGIDNPAITSDQELDDKKMKKKTHKVKEDKKKKKTKNFNPPTRRNWESNYFG MPLQDLVTAEKPIPLFVEKCVEFIEDTGLCTEGLYRVSGNKTDQDNIQKQFDQDHNINLVSMEVTVNAVA GALKAFFADLPDPLIPYSLHPELLEAAKIPDKTERLHALKEIVKKFHPVNYDVFRYVITHLNRVSQQHKI NLMTADNLSICFWPTLMRPDFENREFLSTTKIHQSVVETFIQQCQFFFYNGEIVETTNIVAPPPPSNPGQ LVEPMVPLQLPPPLQPQLIQPQLQTDPLGII TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001173 |
| ORF Size | 4503 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001173.3 |
| RefSeq Size | 9601 bp |
| RefSeq ORF | 4506 bp |
| Locus ID | 394 |
| UniProt ID | Q13017 |
| Domains | RhoGAP, FF |
| Protein Pathways | Focal adhesion, Leukocyte transendothelial migration |
| Gene Summary | Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217131 | ARHGAP5 (Myc-DDK-tagged)-Human Rho GTPase activating protein 5 (ARHGAP5), transcript variant 2 |
CNY 10,272.00 |
|
| RC217131L3 | Lenti-ORF clone of ARHGAP5 (Myc-DDK-tagged)-Human Rho GTPase activating protein 5 (ARHGAP5), transcript variant 2 |
CNY 16,910.00 |
|
| RC217131L4 | Lenti-ORF clone of ARHGAP5 (mGFP-tagged)-Human Rho GTPase activating protein 5 (ARHGAP5), transcript variant 2 |
CNY 16,910.00 |
|
| SC119374 | ARHGAP5 (untagged)-Human Rho GTPase activating protein 5 (ARHGAP5), transcript variant 2 |
CNY 10,280.00 |
