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MEK5 (MAP2K5) (NM_002757) Human Tagged ORF Clone
CAT＃: RG217115
- TrueORF®
MAP2K5 (tGFP-tagged) - Human mitogen-activated protein kinase kinase 5 (MAP2K5), transcript variant 2
ORF Plasmid: DDK
"NM_002757" in other vectors (6)
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CNY 4,370.00
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热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | HsT17454; MAPKK5; MEK5; PRKMK5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG217115 representing NM_002757
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTGGCTAGCCCTTGGCCCCTTTCCTGCCATGGAGAACCAGGTGCTGGTAATTCGCATCAAGATCC CAAATAGTGGCGCGGTGGACTGGACAGTGCACTCCGGGCCGCAGTTACTCTTCAGGGATGTGCTGGATGT GATAGGCCAGGTTCTGCCTGAAGCAACAACTACAGCATTTGAATATGAAGATGAAGATGGTGATCGAATT ACAGTGAGAAGTGATGAGGAAATGAAGGCAATGCTGTCATATTATTATTCCACAGTAATGGAACAGCAAG TAAATGGACAGTTAATAGAGCCTCTGCAGATATTTCCAAGAGCCTGCAAGCCTCCTGGGGAACGGAACAT ACATGGCCTGAAGGTGAATACTCGGGCCGGACCCTCTCAACACAGCAGCCCAGCAGTCTCAGATTCACTT CCAAGCAATAGCTTAAAGAAGTCTTCTGCTGAACTGAAAAAAATACTAGCCAATGGCCAGATGAATGAAC AAGACATACGATATCGGGACACTCTTGGTCATGGCAACGGAGGCACAGTCTACAAAGCATATCATGTCCC GAGTGGGAAAATATTAGCTGTAAAGGTCATACTACTAGATATTACACTGGAACTTCAGAAGCAAATTATG TCTGAATTGGAAATTCTTTATAAGTGCGATTCATCATATATCATTGGATTTTATGGAGCATTTTTTGTAG AAAACAGGATTTCAATATGTACAGAATTCATGGATGGGGGATCTTTGGATGTATATAGGAAAATGCCAGA ACATGTCCTTGGAAGAATTGCAGTAGCAGTTGTTAAAGGCCTTACTTATTTGTGGAGTTTAAAGATTTTA CATAGAGACGTGAAGCCCTCCAATATGCTAGTAAACACAAGAGGACAGGTTAAGCTGTGTGATTTTGGAG TTAGCACTCAGCTGGTGAATTCTATAGCCAAGACGTATGTTGGAACAAATGCTTATATGGCGCCTGAAAG GATTTCAGGGGAGCAGTATGGAATTCATTCTGATGTCTGGAGCTTAGGAATCTCTTTTATGGAGATTCAG AAAAACCAGGGATCTTTAATGCCTCTCCAGCTTCTGCAGTGCATTGTTGATGAGGATTCGCCCGTCCTTC CAGTTGGAGAGTTCTCGGAGCCATTTGTACATTTCATCACTCAGTGTATGCGAAAACAGCCAAAAGAAAG GCCAGCACCTGAAGAATTGATGGGCCACCCGTTCATCGTGCAGTTCAATGATGGAAATGCCGCCGTGGTG TCCATGTGGGTGTGCCGGGCGCTGGAGGAGAGGCGGAGCCAGCAGGGGCCCCCG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217115 representing NM_002757
Red=Cloning site Green=Tags(s) MLWLALGPFPAMENQVLVIRIKIPNSGAVDWTVHSGPQLLFRDVLDVIGQVLPEATTTAFEYEDEDGDRI TVRSDEEMKAMLSYYYSTVMEQQVNGQLIEPLQIFPRACKPPGERNIHGLKVNTRAGPSQHSSPAVSDSL PSNSLKKSSAELKKILANGQMNEQDIRYRDTLGHGNGGTVYKAYHVPSGKILAVKVILLDITLELQKQIM SELEILYKCDSSYIIGFYGAFFVENRISICTEFMDGGSLDVYRKMPEHVLGRIAVAVVKGLTYLWSLKIL HRDVKPSNMLVNTRGQVKLCDFGVSTQLVNSIAKTYVGTNAYMAPERISGEQYGIHSDVWSLGISFMEIQ KNQGSLMPLQLLQCIVDEDSPVLPVGEFSEPFVHFITQCMRKQPKERPAPEELMGHPFIVQFNDGNAAVV SMWVCRALEERRSQQGPP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002757 |
| ORF Size | 1314 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002757.4 |
| RefSeq Size | 2334 bp |
| RefSeq ORF | 1317 bp |
| Locus ID | 5607 |
| UniProt ID | Q13163 |
| Domains | PB1, pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Gap junction, MAPK signaling pathway, Neurotrophin signaling pathway |
| Gene Summary | The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. [provided by RefSeq, May 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217115 | MAP2K5 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase 5 (MAP2K5), transcript variant 2 |
CNY 3,656.00 |
|
| RC217115L3 | Lenti ORF clone of Human mitogen-activated protein kinase kinase 5 (MAP2K5), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC217115L4 | Lenti ORF clone of Human mitogen-activated protein kinase kinase 5 (MAP2K5), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| SC310389 | MAP2K5 (untagged)-Human mitogen-activated protein kinase kinase 5 (MAP2K5), transcript variant 2 |
CNY 7,030.00 |
|
| SC323569 | MAP2K5 (untagged)-Kinase deficient mutant (K195M) of Human mitogen-activated protein kinase kinase 5 (MAP2K5), transcript variant B |
CNY 3,656.00 |
|
| SC323639 | MAP2K5 (untagged)-Kinase deficient mutant (K195M) of Human mitogen-activated protein kinase kinase 5 (MAP2K5), transcript variant B |
CNY 3,656.00 |
