United States
Germany
Japan
United Kingdom
China
ADAM22 (NM_004194) Human Tagged ORF Clone
CAT＃: RG216571
- TrueORF®
ADAM22 (tGFP-tagged) - Human ADAM metallopeptidase domain 22 (ADAM22), transcript variant 4
ORF Plasmid: DDK
"NM_004194" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 10,070.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ADAM 22; DEE61; EIEE61; MDC2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG216571 representing NM_004194
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGCGGCAGTGGCTGTGTCCGTGCCCTTCTTGCTGCTCTGTGTCCTGGGGACCTGCCCTCCGGCGC GCTGCGGCCAGGCAGGAGACGCCTCATTGATGGAGCTAGAGAAGAGGAAGGAAAACCGCTTCGTGGAGCG CCAGAGCATCGTGCCACTGCGCCTCATCTACCGCTCGGGCGGCGAAGACGAAAGTCGGCACGACGCGCTC GACACGCGGGTGCGGGGCGACCTCGGTGGCCCGCAGTTGACTCATGTTGACCAAGCAAGCTTCCAGGTTG ATGCCTTTGGAACGTCATTCATTCTCGATGTCGTGCTAAATCATGATTTGCTGTCCTCTGAATACATAGA GAGACACATTGAACATGGAGGCAAGACTGTGGAAGTTAAAGGAGGAGAGCACTGTTACTACCAGGGCCAT ATCCGAGGAAACCCTGACTCATTTGTTGCATTGTCAACATGCCACGGACTTCATGGGATGTTCTATGACG GGAACCACACATATCTCATTGAGCCAGAAGAAAATGACACTACTCAAGAGGATTTCCATTTTCATTCAGT TTACAAATCCAGACTGTTTGAATTTTCCTTGGATGATCTTCCATCTGAATTTCAGCAAGTAAACATTACT CCATCAAAATTTATTTTGAAGCCAAGACCAAAAAGGAGTAAACGGCAGCTTCGTCGATATCCTCGTAATG TAGAAGAAGAAACCAAATACATTGAACTGATGATTGTGAATGATCACCTTATGTTTAAAAAACATCGGCT TTCCGTTGTACATACCAATACCTATGCGAAATCTGTGGTGAACATGGCAGATTTAATATATAAAGACCAA CTTAAGACCAGGATAGTATTGGTTGCTATGGAAACCTGGGCGACTGACAACAAGTTTGCCATATCTGAAA ATCCATTGATCACCCTACGTGAGTTTATGAAATACAGGAGGGATTTTATCAAAGAGAAAAGTGATGCAGT TCACCTTTTTTCGGGAAGTCAATTTGAGAGTAGCCGGAGCGGGGCAGCTTATATTGGTGGGATTTGCTCG TTGCTGAAAGGAGGAGGCGTGAATGAATTTGGGAAAACTGATTTAATGGCTGTTACACTTGCCCAGTCAT TAGCCCATAATATTGGTATTATCTCAGACAAAAGAAAGTTAGCAAGTGGTGAATGTAAATGCGAGGACAC GTGGTCCGGGTGCATAATGGGAGACACTGGCTATTATCTTCCTAAAAAGTTCACCCAGTGTAATATTGAA GAGTATCATGACTTCCTGAATAGTGGAGGTGGTGCCTGCCTTTTCAACAAACCTTCTAAGCTTCTTGATC CTCCTGAGTGTGGCAATGGCTTCATTGAAACTGGAGAGGAGTGTGATTGTGGAACCCCGGCCGAATGTGT CCTTGAAGGAGCAGAGTGTTGTAAGAAATGCACCTTGACTCAAGACTCTCAATGCAGTGACGGTCTTTGC TGTAAAAAGTGCAAGTTTCAGCCTATGGGCACTGTGTGCCGAGAAGCAGTAAATGATTGTGATATTCGTG AAACGTGCTCAGGAAATTCAAGCCAGTGTGCCCCTAATATTCATAAAATGGATGGATATTCATGTGATGG TGTTCAGGGAATTTGCTTTGGAGGAAGATGCAAAACCAGAGATAGACAATGCAAATACATTTGGGGGCAA AAGGTGACAGCATCAGACAAATATTGCTATGAGAAACTGAATATTGAAGGGACGGAGAAGGGTAACTGTG GGAAAGACAAAGACACATGGATACAGTGCAACAAACGGGATGTGCTTTGTGGTTACCTTTTGTGTACCAA TATTGGCAATATCCCAAGGCTTGGAGAACTCGATGGTGAAATCACATCTACTTTAGTTGTGCAGCAAGGA AGAACATTAAACTGCAGTGGTGGGCATGTTAAGCTTGAAGAAGATGTAGATCTTGGCTATGTGGAAGATG GGACACCTTGTGGTCCCCAAATGATGTGCTTAGAACACAGGTGTCTTCCTGTGGCTTCTTTCAACTTTAG TACTTGCTTGAGCAGTAAAGAAGGCACTATTTGCTCAGGAAATGGAGTTTGCAGTAATGAGCTGAAGTGT GTGTGTAACAGACACTGGATAGGTTCTGATTGCAACACTTACTTCCCTCACAATGATGATGCAAAGACTG GTATCACTCTGTCTGGCAATGGTGTTGCTGGCACCAATATCATAATAGGCATAATTGCTGGCACCATTTT AGTGCTGGCCCTCATATTAGGAATAACTGCGTGGGGTTATAAAAACTATCGAGAACAGAGACAGTTACCC CAGGGAGATTATGTAAAAAAGCCTGGAGATGGTGACTCTTTTTATAGCGACATTCCTCCCGGAGTCAGCA CAAACTCAGCATCTAGTTCTAAGAAGAGGTCAAATGGGCTCTCTCATTCTTGGAGTGAAAGGATTCCAGA CACAAAACATATTTCAGACATCTGTGAAAATGGGCGACCTCGAAGTAACTCTTGGCAAGGTAACCTGGGA GGCAACAAAAAGAAAATCAGAGGCAAAAGATTTAGACCTCGGTCTAATTCAACTGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG216571 representing NM_004194
Red=Cloning site Green=Tags(s) MQAAVAVSVPFLLLCVLGTCPPARCGQAGDASLMELEKRKENRFVERQSIVPLRLIYRSGGEDESRHDAL DTRVRGDLGGPQLTHVDQASFQVDAFGTSFILDVVLNHDLLSSEYIERHIEHGGKTVEVKGGEHCYYQGH IRGNPDSFVALSTCHGLHGMFYDGNHTYLIEPEENDTTQEDFHFHSVYKSRLFEFSLDDLPSEFQQVNIT PSKFILKPRPKRSKRQLRRYPRNVEEETKYIELMIVNDHLMFKKHRLSVVHTNTYAKSVVNMADLIYKDQ LKTRIVLVAMETWATDNKFAISENPLITLREFMKYRRDFIKEKSDAVHLFSGSQFESSRSGAAYIGGICS LLKGGGVNEFGKTDLMAVTLAQSLAHNIGIISDKRKLASGECKCEDTWSGCIMGDTGYYLPKKFTQCNIE EYHDFLNSGGGACLFNKPSKLLDPPECGNGFIETGEECDCGTPAECVLEGAECCKKCTLTQDSQCSDGLC CKKCKFQPMGTVCREAVNDCDIRETCSGNSSQCAPNIHKMDGYSCDGVQGICFGGRCKTRDRQCKYIWGQ KVTASDKYCYEKLNIEGTEKGNCGKDKDTWIQCNKRDVLCGYLLCTNIGNIPRLGELDGEITSTLVVQQG RTLNCSGGHVKLEEDVDLGYVEDGTPCGPQMMCLEHRCLPVASFNFSTCLSSKEGTICSGNGVCSNELKC VCNRHWIGSDCNTYFPHNDDAKTGITLSGNGVAGTNIIIGIIAGTILVLALILGITAWGYKNYREQRQLP QGDYVKKPGDGDSFYSDIPPGVSTNSASSSKKRSNGLSHSWSERIPDTKHISDICENGRPRSNSWQGNLG GNKKKIRGKRFRPRSNSTE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004194 |
| ORF Size | 2577 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004194.4 |
| RefSeq Size | 2880 bp |
| RefSeq ORF | 2580 bp |
| Locus ID | 53616 |
| UniProt ID | Q9P0K1 |
| Domains | Reprolysin, DISIN, Pep_M12B_propep, ACR |
| Protein Families | Druggable Genome, Protease, Transmembrane |
| Gene Summary | This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Unlike other members of the ADAM protein family, the protein encoded by this gene lacks metalloprotease activity since it has no zinc-binding motif. This gene is highly expressed in the brain and may function as an integrin ligand in the brain. In mice, it has been shown to be essential for correct myelination in the peripheral nervous system. Alternative splicing results in several transcript variants.[provided by RefSeq, Dec 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216571 | ADAM22 (Myc-DDK-tagged)-Human ADAM metallopeptidase domain 22 (ADAM22), transcript variant 4 |
CNY 9,120.00 |
|
| RC216571L3 | Lenti-ORF clone of ADAM22 (Myc-DDK-tagged)-Human ADAM metallopeptidase domain 22 (ADAM22), transcript variant 4 |
CNY 11,020.00 |
|
| RC216571L4 | Lenti-ORF clone of ADAM22 (mGFP-tagged)-Human ADAM metallopeptidase domain 22 (ADAM22), transcript variant 4 |
CNY 11,020.00 |
|
| SC314828 | ADAM22 (untagged)-Human ADAM metallopeptidase domain 22 (ADAM22), transcript variant 4 |
CNY 16,630.00 |
