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GABA A Receptor alpha 5 (GABRA5) (NM_000810) Human Tagged ORF Clone
CAT＃: RG216541
- TrueORF®
GABRA5 (tGFP-tagged) - Human gamma-aminobutyric acid (GABA) A receptor, alpha 5 (GABRA5), transcript variant 1
ORF Plasmid: DDK
"NM_000810" in other vectors (6)
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CNY 4,370.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DEE79; EIEE79 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG216541 representing NM_000810
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAATGGAATGTTCTCTGGTTTTATCATGATCAAAAACCTCCTTCTCTTTTGTATTTCCATGAACT TATCCAGTCACTTTGGCTTTTCACAGATGCCAACCAGTTCAGTGAAAGATGAGACCAATGACAACATCAC GATATTTACCAGGATCTTGGATGGGCTCTTGGATGGCTACGACAACAGACTTCGGCCCGGGCTGGGAGAG CGCATCACTCAGGTGAGGACCGACATCTACGTCACCAGCTTCGGCCCGGTGTCCGACACGGAAATGGAGT ACACCATAGACGTGTTTTTCCGACAAAGCTGGAAAGATGAAAGGCTTCGGTTTAAGGGGCCCATGCAGCG CCTCCCTCTCAACAACCTCCTTGCCAGCAAGATCTGGACCCCAGACACGTTCTTCCACAACGGGAAGAAG TCCATCGCTCACAACATGACCACGCCCAACAAGCTGCTGCGGCTGGAGGACGACGGCACCCTGCTCTACA CCATGCGCTTGACCATCTCTGCAGAGTGCCCCATGCAGCTTGAGGACTTCCCGATGGATGCGCACGCTTG CCCTCTGAAATTTGGCAGCTATGCGTACCCTAATTCTGAAGTCGTCTACGTCTGGACCAACGGCTCCACC AAGTCGGTGGTGGTGGCGGAAGATGGCTCCAGACTGAACCAGTACCACCTGATGGGGCAGACGGTGGGCA CTGAGAACATCAGCACCAGCACAGGCGAATACACAATCATGACAGCTCACTTCCACCTGAAAAGGAAGAT TGGCTACTTTGTCATCCAGACCTACCTTCCCTGCATAATGACCGTGATCTTATCACAGGTGTCCTTTTGG CTGAACCGGGAATCAGTCCCAGCCAGGACAGTTTTTGGGGTCACCACGGTGCTGACCATGACGACCCTCA GCATCAGCGCCAGGAACTCTCTGCCCAAAGTGGCCTACGCCACCGCCATGGACTGGTTCATAGCCGTGTG CTATGCCTTCGTCTTCTCGGCGCTGATAGAGTTTGCCACGGTCAATTACTTTACCAAGAGAGGCTGGGCC TGGGATGGCAAAAAAGCCTTGGAAGCAGCCAAGATCAAGAAAAAGCGTGAAGTCATACTAAATAAGTCAA CAAACGCTTTTACAACTGGGAAGATGTCTCACCCCCCAAACATTCCGAAGGAACAGACCCCAGCAGGGAC GTCGAATACAACCTCAGTCTCAGTAAAACCCTCTGAAGAGAAGACTTCTGAAAGCAAAAAGACTTACAAC AGTATCAGCAAAATTGACAAAATGTCCCGAATCGTATTCCCAGTCTTGTTCGGCACTTTCAACTTAGTTT ACTGGGCAACGTATTTGAATAGGGAGCCGGTGATAAAAGGAGCCGCCTCTCCAAAA AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG216541 representing NM_000810
Red=Cloning site Green=Tags(s) MDNGMFSGFIMIKNLLLFCISMNLSSHFGFSQMPTSSVKDETNDNITIFTRILDGLLDGYDNRLRPGLGE RITQVRTDIYVTSFGPVSDTEMEYTIDVFFRQSWKDERLRFKGPMQRLPLNNLLASKIWTPDTFFHNGKK SIAHNMTTPNKLLRLEDDGTLLYTMRLTISAECPMQLEDFPMDAHACPLKFGSYAYPNSEVVYVWTNGST KSVVVAEDGSRLNQYHLMGQTVGTENISTSTGEYTIMTAHFHLKRKIGYFVIQTYLPCIMTVILSQVSFW LNRESVPARTVFGVTTVLTMTTLSISARNSLPKVAYATAMDWFIAVCYAFVFSALIEFATVNYFTKRGWA WDGKKALEAAKIKKKREVILNKSTNAFTTGKMSHPPNIPKEQTPAGTSNTTSVSVKPSEEKTSESKKTYN SISKIDKMSRIVFPVLFGTFNLVYWATYLNREPVIKGAASPK SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000810 |
| ORF Size | 1386 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000810.2, NP_000801.1 |
| RefSeq Size | 2352 bp |
| RefSeq ORF | 1389 bp |
| Locus ID | 2558 |
| UniProt ID | P31644 |
| Domains | Neur_chan_memb, Neur_chan_LBD |
| Protein Families | Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane |
| Protein Pathways | Neuroactive ligand-receptor interaction |
| Gene Summary | GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Transcript variants utilizing three different alternative non-coding first exons have been described. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216541 | GABRA5 (Myc-DDK-tagged)-Human gamma-aminobutyric acid (GABA) A receptor, alpha 5 (GABRA5), transcript variant 1 |
CNY 3,656.00 |
|
| RC216541L1 | Lenti ORF clone of Human gamma-aminobutyric acid (GABA) A receptor, alpha 5 (GABRA5), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC216541L2 | Lenti ORF clone of Human gamma-aminobutyric acid (GABA) A receptor, alpha 5 (GABRA5), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC216541L3 | Lenti ORF clone of Human gamma-aminobutyric acid (GABA) A receptor, alpha 5 (GABRA5), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC216541L4 | Lenti ORF clone of Human gamma-aminobutyric acid (GABA) A receptor, alpha 5 (GABRA5), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| SC119626 | GABRA5 (untagged)-Human gamma-aminobutyric acid (GABA) A receptor, alpha 5 (GABRA5), transcript variant 1 |
CNY 3,656.00 |
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