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CDC2L1 (CDK11B) (NM_033492) Human Tagged ORF Clone
CAT＃: RG215881
- TrueORF®
CDK11B (tGFP-tagged) - Human cyclin-dependent kinase 11B (CDK11B), transcript variant 8. Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_033492" in other vectors (4)
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CNY 8,550.00
Product images
CNY 2,700.00
CNY 3,790.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CDC2L1; CDK11; CDK11-p46; CDK11-p58; CDK11-p110; CLK-1; p58; p58CDC2L1; p58CLK-1; PK58 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG215881 ORF sequence, codon optimized.
Due to the complexity of NM_033492, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGATGAAAAAGATTCATGGAAGGTGAAGACCCTCGATGAGATCCTTCAGGAAAAGAAACGCCGCA AAGAGCAAGAAGAGAAAGCGGAGATCAAGCGACTGAAAAACTCTGATGATCGGGATTCCAAGAGGGACTC CCTGGAGGAGGGAGAGTTGCGGGACCACTGTATGGAAATAACAATCAGGAACAGCCCATATCGACGGGAA GACAGCATGGAAGACAGGGGCGAAGAAGACGATTCCCTCGCCATCAAGCCTCCCCAGCAAATGTCCAGAA AGGAGAAAGTGCACCATAGGAAGGATGAGAAGCGGAAGGAAAAAAAACACGCCCGAGTCAAAGAAAAAGA GAGAGAGCACGAGAGAAGAAAACGGCACCGCGAGGAGCAGGATAAAGCGCGGCGAGAATGGGAAAGACAG AAAAGAAGAGAGATGGCGCGGGAACATTCAAGAAGGGAAAGGGGTAATGACGGGGTTTGCTTGTTCCGCG ATCGGCTCGAACAGCTCGAAAGAAAGAGGGAAAGAGAAAGAAAGATGAGAGAACAGCAGAAGGAACAGAG AGAACAAAAAGAGAGAGAGAGGCGCGCCGAGGAGAGGAGGAAGGAGAGGGAAGCCCGCAGAGAAGTCAGC GCCCACCATAGAACTATGAGAGAGGACTACAGCGACAAAGTCAAGGCATCACATTGGAGCCGCAGCCCTC CACGACCACCTAGAGAAAGGTTCGAGTTGGGCGATGGAAGAAAACCAGTTAAAGAGGAAAAAATGGAGGA GAGAGATCTGCTTAGCGATCTCCAGGACATCAGTGATAGCGAACGAAAAACTAGCTCCGCTGAGTCATCA TCTGCAGAGAGCGGTTCAGGATCCGAGGAGGAGGAGGAAGAAGAAGAGGAGGAAGAGGAGGAGGGGAGCA CGAGCGAGGAGTCCGAGGAGGAAGAGGAAGAGGAGGAGGAGGAAGAAGAAGAGACAGGGAGTAACTCCGA GGAGGCCTCTGAGCAGTCAGCCGAGGAAGTCTCAGAAGAGGAGATGTCCGAAGACGAGGAGCGGGAGAAT GAGAACCATTTGCTGGTGGTCCCAGAATCACGCTTTGATAGGGATTCTGGAGAGTCCGAGGAAGCTGAAG AAGAAGTGGGGGAAGGTACACCACAGTCATCCGCACTGACCGAGGGCGACTATGTGCCGGATTCCCCAGC CCTGAGCCCAATTGAGCTGAAGCAGGAACTGCCCAAATATCTGCCCGCCCTGCAGGGGTGCCGGTCAGTT GAGGAATTCCAGTGCCTCAACAGAATTGAGGAAGGGACGTACGGTGTGGTCTACCGAGCTAAAGACAAAA AGACTGACGAAATCGTCGCTCTGAAGCGGCTGAAGATGGAAAAAGAGAAAGAGGGGTTCCCTATCACGTC CCTGAGGGAGATCAATACAATTTTGAAGGCCCAGCACCCGAACATTGTAACAGTGCGGGAGATCGTGGTG GGAAGTAATATGGATAAGATTTATATCGTCATGAACTACGTTGAACACGATCTGAAGAGCTTGATGGAAA CTATGAAGCAACCATTTCTGCCAGGAGAGGTGAAGACCCTGATGATACAACTGTTGAGAGGGGTGAAGCA CCTGCACGATAACTGGATCCTGCACAGAGATCTGAAAACCTCCAACCTCCTGCTTTCACATGCTGGTATC CTGAAAGTTGGGGATTTCGGACTGGCAAGGGAGTACGGATCACCTCTCAAGGCTTATACCCCGGTGGTGG TGACCCTCTGGTACCGGGCACCAGAACTGCTGTTGGGAGCTAAAGAGTATTCAACGGCCGTGGATATGTG GTCCGTCGGATGCATCTTCGGCGAACTCCTCACACAAAAACCCCTGTTCCCCGGGAAAAGTGAGATTGAT CAGATCAACAAAGTCTTCAAAGACCTGGGGACCCCCAGCGAAAAGATCTGGCCGGGGTATAGCGAACTGC CTGCTGTTAAAAAAATGACCTTCTCCGAGCATCCATACAATAACCTCCGGAAGAGGTTTGGGGCCCTGCT GAGTGATCAGGGCTTCGACCTGATGAATAAATTTCTTACTTACTTTCCCGGACGCCGCATTAGTGCCGAA GACGGCCTCAAGCACGAATACTTTCGCGAAACTCCACTGCCCATCGACCCTTCCATGTTTCCGACCTGGC CAGCAAAAAGCGAACAGCAAAGGGTGAAGAGAGGCACTTCTCCGCGCCCGCCGGAGGGGGGTCTTGGCTA CAGCCAACTGGGCGACGATGATCTGAAGGAGACAGGCTTCCATTTGACTACTACAAACCAGGGCGCTAGC GCTGCTGGACCAGGGTTCAGCCTCAAGTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG215881 representing NM_033492
Red=Cloning site Green=Tags(s) MGDEKDSWKVKTLDEILQEKKRRKEQEEKAEIKRLKNSDDRDSKRDSLEEGELRDHCMEITIRNSPYRRE DSMEDRGEEDDSLAIKPPQQMSRKEKVHHRKDEKRKEKKHARVKEKEREHERRKRHREEQDKARREWERQ KRREMAREHSRRERGNDGVCLFRDRLEQLERKRERERKMREQQKEQREQKERERRAEERRKEREARREVS AHHRTMREDYSDKVKASHWSRSPPRPPRERFELGDGRKPVKEEKMEERDLLSDLQDISDSERKTSSAESS SAESGSGSEEEEEEEEEEEEEGSTSEESEEEEEEEEEEEEETGSNSEEASEQSAEEVSEEEMSEDEEREN ENHLLVVPESRFDRDSGESEEAEEEVGEGTPQSSALTEGDYVPDSPALSPIELKQELPKYLPALQGCRSV EEFQCLNRIEEGTYGVVYRAKDKKTDEIVALKRLKMEKEKEGFPITSLREINTILKAQHPNIVTVREIVV GSNMDKIYIVMNYVEHDLKSLMETMKQPFLPGEVKTLMIQLLRGVKHLHDNWILHRDLKTSNLLLSHAGI LKVGDFGLAREYGSPLKAYTPVVVTLWYRAPELLLGAKEYSTAVDMWSVGCIFGELLTQKPLFPGKSEID QINKVFKDLGTPSEKIWPGYSELPAVKKMTFSEHPYNNLRKRFGALLSDQGFDLMNKFLTYFPGRRISAE DGLKHEYFRETPLPIDPSMFPTWPAKSEQQRVKRGTSPRPPEGGLGYSQLGDDDLKETGFHLTTTNQGAS AAGPGFSLKF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033492 |
| ORF Size | 2340 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_033492.1, NP_277027.1 |
| RefSeq Size | 2480 bp |
| RefSeq ORF | 2342 bp |
| Locus ID | 984 |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | This gene encodes a member of the serine/threonine protein kinase family. Members of this kinase family are known to be essential for eukaryotic cell cycle control. Due to a segmental duplication, this gene shares very high sequence identity with a neighboring gene. These two genes are frequently deleted or altered in neuroblastoma. The protein kinase encoded by this gene can be cleaved by caspases and may play a role in cell apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215881 | CDK11B (Myc-DDK-tagged)-Human cyclin-dependent kinase 11B (CDK11B), transcript variant 8. Note: ORF is codon optimized |
CNY 5,712.00 |
|
| RC215881L3 | Lenti-ORF clone of CDK11B (Myc-DDK-tagged)-Human cyclin-dependent kinase 11B (CDK11B), transcript variant 8. Note: ORF is codon optimized |
CNY 9,690.00 |
|
| RC215881L4 | Lenti-ORF clone of CDK11B (mGFP-tagged)-Human cyclin-dependent kinase 11B (CDK11B), transcript variant 8. Note: ORF is codon optimized |
CNY 9,690.00 |
|
| SC309470 | CDK11B (untagged)-Human cyclin-dependent kinase 11B (CDK11B), transcript variant 8 |
CNY 15,110.00 |
