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IRAG1 (NM_001100163) Human Tagged ORF Clone
CAT＃: RG215686
- TrueORF®
MRVI1 (tGFP-tagged) - Human murine retrovirus integration site 1 homolog (MRVI1), transcript variant 3
ORF Plasmid: DDK
"NM_001100163" in other vectors (4)
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CNY 9,030.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | IRAG; JAW1L; MRVI1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG215686 representing NM_001100163
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC CTGACTGGGGATGCCACTTCACCAGAAGGAGAAACCGACAAAAACCTGGCCAACAGAGTTCACAGTCCCC ACAAGAGGCTTTCTCACCGACACTTGAAGGTGTCCACTGCCTCCCTGACATCTGTGGACCCCGCGGGGCA CATCATTGACCTGGTGAATGACCAGCTGCCAGACATCAGCATCTCAGAGGAGGACAAGAAGAAAAACCTG GCGCTGCTGGAAGAAGCCAAGTTGGTGAGTGAGCGATTCCTGACCCGCCGTGGGAGGAAGTCCAGGAGCA GCCCCGGAGACTCCCCATCAGCTGTTTCCCCGAACCTCAGCCCCAGCGCTTCTCCTACATCCTCTCGGAG CAACTCACTTACAGTCCCCACCCCGCCAGGTTTGGATGTGTGCAGTGGCCCGCCATCCCCTCTGCCTGGA GCACCACCACAGAAGGGGGATGAGGCCGACGTCTCTTCACCTCACCCTGGCGAGCCTAACGTCCCCAAAG GGCTAGCTGACAGGAAGCAGAATGACCAGAGGAAAGTGTCTCAGGGCAGGCTGGCTCCTCGTCCTCCTCC AGTTGAGAAGTCCAAAGAGATTGCAATAGAACAAAAGGAAAACTTCGATCCCCTCCAGTACCCCGAGACC ACACCCAAAGGCCTAGCTCCTGTTACAAACAGCAGTGGGAAAATGGCCCTGAACAGCCCTCAGCCTGGCC CCGTGGAGAGCGAGCTGGGGAAGCAGCTCTTGAAAACGGGCTGGGAGGGCAGCCCTCTGCCGAGAAGTCC AACCCAGGATGCGGCAGGAGTGGGTCCCCCAGCCTCCCAGGGGAGAGGCCCAGCTGGAGAGCCGATGGGG CCCGAGGCTGGCTCCAAAGCTGAGCTTCCACCCACTGTGTCCCGGCCCCCGCTGCTGCGAGGGCTCTCCT GGGACAGTGGCCCTGAAGAACCTGGCCCCCGGCTGCAGAAAGTGCTTGCCAAGCTGCCACTGGCAGAGGA AGAAAAGCGTTTTGCAGGCAAGGCCGGCGGCAAGCTGGCCAAGGCCCCTGGTCTCAAAGACTTTCAGATA CAAGTGCAGCCCGTGCGGATGCAGAAACTGACCAAGCTCCGAGAGGAGCACATCCTGATGAGAAATCAGA ACTTAGTGGGGCTCAAGCTTCCAGACCTTAGTGAAGCAGCTGAGCAGGAAAAAGGGCTTCCTTCTGAACT CTCCCCAGCTATTGAGGAAGAAGAGTCAAAGAGTGGCTTAGATGTCATGCCTAATATTTCTGATGTGCTG CTGCGCAAACTGCGGGTCCACAGGAGTCTCCCTGGAAGTGCCCCTCCACTCACTGAAAAGGAAGTTGAGA ACGTGTTTGTGCAACTGTCCTTGGCCTTTAGAAATGACAGCTACACTCTGGAATCTAGAATTAACCAGGC TGAAAGGGAACGCAACCTGACAGAGGAGAACACTGAGAAAGAACTGGAAAACTTCAAAGCTTCCATTACG TCCTCAGCTTCACTCTGGCACCACTGTGAGCACCGGGAAACCTACCAGAAGTTGCTGGAGGACATCGCTG TCCTGCACCGCCTGGCTGCCCGCCTCTCCAGCCGAGCTGAGGTGGTAGGCGCCGTCCGCCAGGAAAAGCG CATGTCGAAAGCAACGGAAGTGATGATGCAGTATGTGGAGAATCTAAAGAGGACGTATGAGAAGGACCAT GCGGAGCTCATGGAGTTTAAAAAGCTTGCAAATCAGAATTCAAGCCGCAGCTGTGGCCCCTCTGAAGATG GGGTCCCTCGCACGGCACGGTCCATGTCCCTCACGCTGGGAAAGAATATGCCTCGCCGGAGGGTCAGCGT TGCTGTGGTTCCTAAGTTTAATGCCCTGAATCTGCCTGGCCAAACTCCCAGCTCATCATCCATTCCCTCC TTACCAGCCTTGTCGGAATCACCCAATGGGAAAGGCAGCCTACCTGTCACTTCAGCACTGCCTGCACTTT TGGAAAATGGAAAGACAAATGGGGACCCAGATTGTGAAGCCTCTGCTCCTGCGCTGACCCTGAGCTGCCT GGAGGAGCTTAGTCAGGAGACCAAGGCCAGGATGGAGGAAGAAGCCTACAGCAAGGGATTCCAAGAAGGT CTAAAGAAGACCAAAGAACTTCAAGACCTGAAGGAGGAGGAGGAAGAACAGAAGAGTGAGAGTCCTGAGG AACCTGAAGAGGTAGAAGAAACTGAGGAAGAGGAAAAGGGCCCAAGAAGCAGCAAACTTGAAGAATTGGT CCATTTCTTACAAGTCATGTATCCCAAACTGTGTCAGCACTGGCAAGTGATCTGGATGATGGCTGCAGTG ATGCTGGTCTTGACTGTTGTGCTGGGGCTCTACAATTCCTATAACTCTTGTGCAGAGCAGGCTGATGGGC CCCTTGGAAGATCCACTTGCTCGGCAGCCCAGAGGGACTCCTGGTGGAGCTCAGGACTCCAGCATGAGCA GCCTACAGAGCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG215686 representing NM_001100163
Red=Cloning site Green=Tags(s) LTGDATSPEGETDKNLANRVHSPHKRLSHRHLKVSTASLTSVDPAGHIIDLVNDQLPDISISEEDKKKNL ALLEEAKLVSERFLTRRGRKSRSSPGDSPSAVSPNLSPSASPTSSRSNSLTVPTPPGLDVCSGPPSPLPG APPQKGDEADVSSPHPGEPNVPKGLADRKQNDQRKVSQGRLAPRPPPVEKSKEIAIEQKENFDPLQYPET TPKGLAPVTNSSGKMALNSPQPGPVESELGKQLLKTGWEGSPLPRSPTQDAAGVGPPASQGRGPAGEPMG PEAGSKAELPPTVSRPPLLRGLSWDSGPEEPGPRLQKVLAKLPLAEEEKRFAGKAGGKLAKAPGLKDFQI QVQPVRMQKLTKLREEHILMRNQNLVGLKLPDLSEAAEQEKGLPSELSPAIEEEESKSGLDVMPNISDVL LRKLRVHRSLPGSAPPLTEKEVENVFVQLSLAFRNDSYTLESRINQAERERNLTEENTEKELENFKASIT SSASLWHHCEHRETYQKLLEDIAVLHRLAARLSSRAEVVGAVRQEKRMSKATEVMMQYVENLKRTYEKDH AELMEFKKLANQNSSRSCGPSEDGVPRTARSMSLTLGKNMPRRRVSVAVVPKFNALNLPGQTPSSSSIPS LPALSESPNGKGSLPVTSALPALLENGKTNGDPDCEASAPALTLSCLEELSQETKARMEEEAYSKGFQEG LKKTKELQDLKEEEEEQKSESPEEPEEVEETEEEEKGPRSSKLEELVHFLQVMYPKLCQHWQVIWMMAAV MLVLTVVLGLYNSYNSCAEQADGPLGRSTCSAAQRDSWWSSGLQHEQPTEQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001100163 |
| ORF Size | 2463 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001100163.3 |
| RefSeq Size | 6323 bp |
| RefSeq ORF | 2466 bp |
| Locus ID | 10335 |
| UniProt ID | Q9Y6F6 |
| Protein Families | Transmembrane |
| Protein Pathways | Vascular smooth muscle contraction |
| Gene Summary | This gene is similar to a putative mouse tumor suppressor gene (Mrvi1) that is frequently disrupted by mouse AIDS-related virus (MRV). The encoded protein, which is found in the membrane of the endoplasmic reticulum, is similar to Jaw1, a lymphoid-restricted protein whose expression is down-regulated during lymphoid differentiation. This protein is a substrate of cGMP-dependent kinase-1 (PKG1) that can function as a regulator of IP3-induced calcium release. Studies in mouse suggest that MRV integration at Mrvi1 induces myeloid leukemia by altering the expression of a gene important for myeloid cell growth and/or differentiation, and thus this gene may function as a myeloid leukemia tumor suppressor gene. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene, and alternative translation start sites, including a non-AUG (CUG) start site, are used. [provided by RefSeq, May 2011] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215686 | MRVI1 (Myc-DDK-tagged)-Human murine retrovirus integration site 1 homolog (MRVI1), transcript variant 3 |
CNY 8,170.00 |
|
| RC215686L3 | Lenti-ORF clone of MRVI1 (Myc-DDK-tagged)-Human murine retrovirus integration site 1 homolog (MRVI1), transcript variant 3 |
CNY 10,070.00 |
|
| RC215686L4 | Lenti-ORF clone of MRVI1 (mGFP-tagged)-Human murine retrovirus integration site 1 homolog (MRVI1), transcript variant 3 |
CNY 10,070.00 |
|
| SC316838 | MRVI1 (untagged)-Human murine retrovirus integration site 1 homolog (MRVI1), transcript variant 3 |
CNY 7,220.00 |
