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PTPN22 (NM_012411) Human Tagged ORF Clone
CAT＃: RG215662
- TrueORF®
PTPN22 (tGFP-tagged) - Human protein tyrosine phosphatase, non-receptor type 22 (lymphoid) (PTPN22), transcript variant 2
ORF Plasmid: DDK
"NM_012411" in other vectors (4)
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CNY 6,370.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | LYP; LYP1; LYP2; PEP; PTPN8; PTPN22.5; PTPN22.6 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG215662 representing NM_012411
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCAAAGAGAAATTCTGCAGAAGTTCCTGGATGAGGCCCAAAGCAAGAAAATTACTAAAGAGGAGT TTGCCAATGAATTTCTGAAGCTGAAAAGGCAATCTACCAAGTACAAGGCAGACAAAACCTATCCTACAAC TGTGGCTGAGAATGCCAAGAATATCAAGAAAAACAGATATAAGGATATTTTGCCCTATGATTATAGCCGG GTAGAACTATCCCTGATAACCTCTGATGAGGATTCCAGCTACATCAATGCCAACTTCATTAAGGGAGTTT ATGGACCCAAGGCTTATATTGCCACCCAGGGTCCTTTATCTACAACCCTCCTGGACTTCTGGAGGATGAT TTGGGAATATAGTGTCCTTATCATTGTTATGGCATGCATGGAGTATGAAATGGGAAAGAAAAAGTGTGAG CGCTACTGGGCTGAGCCAGGAGAGATGCAGCTGGAATTTGGCCCTTTCTCTGTATCCTGTGAAGCTGAAA AAAGGAAATCTGATTATATAATCAGGACTCTAAAAGTTAAGTTCAATAGTGAAACTCGAACTATCTACCA GTTTCATTACAAGAATTGGCCAGACCATGATGTACCTTCATCTATAGACCCTATTCTTGAGCTCATCTGG GATGTACGTTGTTACCAAGAGGATGACAGTGTTCCCATATGCATTCACTGCAGTGCTGGCTGTGGAAGGA CTGGTGTTATTTGTGCTATTGATTATACATGGATGTTGCTAAAAGATGGGATAATTCCTGAGAACTTCAG TGTTTTCAGTTTGATCCGGGAAATGCGGACACAGAGGCCTTCATTAGTTCAAACGCAGGAACAATATGAA CTGGTCTACAATGCTGTATTAGAACTATTTAAGAGACAGATGGATGTTATCAGAGATAAACATTCTGGAA CAGAGAGTCAAGCAAAGCATTGTATTCCTGAGAAAAATCACACTCTCCAAGCAGACTCTTATTCTCCTAA TTTACCAAAAAGTACCACAAAAGCAGCAAAAATGATGAACCAACAAAGGACAAAAATGGAAATCAAAGAA TCTTCTTCCTTTGACTTTAGGACTTCTGAAATAAGTGCAAAAGAAGAGCTAGTTTTGCACCCTGCTAAAT CAAGCACTTCTTTTGACTTTCTGGAGCTAAATTACAGTTTTGACAAAAATGCTGACACAACCATGAAATG GCAGACAAAGGCATTTCCAATAGTTGGGGAGCCTCTTCAGAAGCATCAAAGTTTGGATTTGGGCTCTCTT TTGTTTGAGGGATGTTCTAATTCTAAACCTGTAAATGCAGCAGGAAGATATTTTAATTCAAAGGTGCCAA TAACACGGACCAAATCAACTCCTTTTGAATTGATACAGCAGAGAGAAACCAAGGAGGTGGACAGCAAGGA AAACTTTTCTTATTTGGAATCTCAACCACATGATTCTTGTTTTGTAGAGATGCAGGCTCAAAAAGTAATG CATGTTTCTTCAGCAGAACTGAATTATTCACTGCCATATGACTCTAAACACCAAATACGTAATGCCTCTA ATGTAAAGCACCATGACTCTAGTGCTCTTGGTGTATATTCTTACATACCTTTAGTGGAAAATCCTTATTT TTCATCATGGCCTCCAAGTGGTACCAGTTCTAAGATGTCTCTTGATTTACCTGAGAAGCAAGATGGAACT GTTTTTCCTTCTTCTCTGTTGCCAACATCCTCTACATCCCTCTTCTCTTATTACAATTCACATGATTCTT TATCACTGAATTCTCCAACCAATATTTCCTCACTATTGAACCAGGAGTCAGCTGTACTAGCAACTGCTCC AAGGATAGATGATGAAATCCCCCCTCCACTTCCTGTACGGACACCTGAATCATTTATTGTGGTTGAGGAA GCTGGAGAATTCTCACCAAATGTTCCCAAATCCTTATCCTCAGCTGTGAAGGTAAAAATTGGAACATCAC TGGAATGGGGTGGAACATCTGAACCAAAGAAATTTGATGACTCTGTGATACTTAGACCAAGCAAGAGTGT AAAACTCCGAAGTCCTAAATCAGGTAAAAATTTCTCTTGGCTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG215662 representing NM_012411
Red=Cloning site Green=Tags(s) MDQREILQKFLDEAQSKKITKEEFANEFLKLKRQSTKYKADKTYPTTVAENAKNIKKNRYKDILPYDYSR VELSLITSDEDSSYINANFIKGVYGPKAYIATQGPLSTTLLDFWRMIWEYSVLIIVMACMEYEMGKKKCE RYWAEPGEMQLEFGPFSVSCEAEKRKSDYIIRTLKVKFNSETRTIYQFHYKNWPDHDVPSSIDPILELIW DVRCYQEDDSVPICIHCSAGCGRTGVICAIDYTWMLLKDGIIPENFSVFSLIREMRTQRPSLVQTQEQYE LVYNAVLELFKRQMDVIRDKHSGTESQAKHCIPEKNHTLQADSYSPNLPKSTTKAAKMMNQQRTKMEIKE SSSFDFRTSEISAKEELVLHPAKSSTSFDFLELNYSFDKNADTTMKWQTKAFPIVGEPLQKHQSLDLGSL LFEGCSNSKPVNAAGRYFNSKVPITRTKSTPFELIQQRETKEVDSKENFSYLESQPHDSCFVEMQAQKVM HVSSAELNYSLPYDSKHQIRNASNVKHHDSSALGVYSYIPLVENPYFSSWPPSGTSSKMSLDLPEKQDGT VFPSSLLPTSSTSLFSYYNSHDSLSLNSPTNISSLLNQESAVLATAPRIDDEIPPPLPVRTPESFIVVEE AGEFSPNVPKSLSSAVKVKIGTSLEWGGTSEPKKFDDSVILRPSKSVKLRSPKSGKNFSWL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012411 |
| ORF Size | 2073 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012411.2, NP_036543.2 |
| RefSeq Size | 2289 bp |
| RefSeq ORF | 2259 bp |
| Locus ID | 26191 |
| UniProt ID | Q9Y2R2 |
| Domains | Y_phosphatase, PTPc_motif |
| Protein Families | Druggable Genome, Phosphatase |
| Gene Summary | This gene encodes of member of the non-receptor class 4 subfamily of the protein-tyrosine phosphatase family. The encoded protein is a lymphoid-specific intracellular phosphatase that associates with the molecular adapter protein CBL and may be involved in regulating CBL function in the T-cell receptor signaling pathway. Mutations in this gene may be associated with a range of autoimmune disorders including Type 1 Diabetes, rheumatoid arthritis, systemic lupus erythematosus and Graves' disease. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Mar 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215662 | PTPN22 (Myc-DDK-tagged)-Human protein tyrosine phosphatase, non-receptor type 22 (lymphoid) (PTPN22), transcript variant 2 |
CNY 5,512.00 |
|
| RC215662L3 | Lenti ORF clone of Human protein tyrosine phosphatase, non-receptor type 22 (lymphoid) (PTPN22), transcript variant 2, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC215662L4 | Lenti ORF clone of Human protein tyrosine phosphatase, non-receptor type 22 (lymphoid) (PTPN22), transcript variant 2, mGFP tagged |
CNY 7,700.00 |
|
| SC303978 | PTPN22 (untagged)-Human protein tyrosine phosphatase, non-receptor type 22 (lymphoid) (PTPN22), transcript variant 2 |
CNY 13,400.00 |
